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The map label for this gene is purN [H]

Identifier: 146283166

GI number: 146283166

Start: 3052878

End: 3053525

Strand: Direct

Name: purN [H]

Synonym: PST_2831

Alternate gene names: 146283166

Gene position: 3052878-3053525 (Clockwise)

Preceding gene: 146283165

Following gene: 146283167

Centisome position: 66.84

GC content: 65.74

Gene sequence:

>648_bases
ATGGGCACGACCTGCAACGTAGTGGTGCTGATTTCGGGTTCCGGCAGCAACCTGCAGGCACTGATCGATAGCCAGCACGA
AGGCAACCCGGCGCGCATTCGCGCCGTGATTGCCAACCGCGTCGATGCCTTCGGCCTGACCCGGGCCAAGGGCGCCGGCA
TCCCCACCGCCGTTCTGGATCACAAGGCGTTCGATGGCCGCGAGGCGTTCGATGCCGCGCTGATGGAACTGATCGACGCC
CATGCCCCGGATCTGGTGATCCTGGCGGGCTTCATGCGCATCCTCAGCCCTGGCTTTGTCCGTCACTACCACGGCCGCCT
GCTCAACATCCATCCGTCGCTGCTGCCCAAGTACAAGGGGCTCGACACCCATCGCCGCGCGCTTGAGGCGGGCGACGCGG
AGCATGGCTGCAGCGTGCACTTCGTGACCGAGGAATTGGACGGCGGCCCGGTGGTCCTCCAGGCCGCCCTGCAGGTCAAA
CCAGGCGACGACATCGAAAGCCTGACCCAGCGGGTGCATGTGGCCGAACACCAGATCTACCCGCTGGCCATGCGCTGGTT
CGCCGAGGGGCGCTTGCGCCTCGCCGAACAAGGCGCGATGCTGGACGGCGTCACCCTGCCGGTCTCCGGCTATCAGATCA
AAATCTAG

Upstream 100 bases:

>100_bases
ACCTGCGCGCATCCGGTGAGGCACCGTGGGTCATCGGCCAGATCGCCACTGCAGCGCAGGGCGCGGAACGCGTCGTACTG
AACAACCTGAAACAGCATTG

Downstream 100 bases:

>100_bases
GAGATACCATGCGTCGCGCCTTGCTGCTTGCTCTCGCCGTGCTGGCCCTTCCGGTCCAGGCCTTGGAGCTCAAGCCCTTC
TCCGCCAGCTACACCGCCGA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MGTTCNVVVLISGSGSNLQALIDSQHEGNPARIRAVIANRVDAFGLTRAKGAGIPTAVLDHKAFDGREAFDAALMELIDA
HAPDLVILAGFMRILSPGFVRHYHGRLLNIHPSLLPKYKGLDTHRRALEAGDAEHGCSVHFVTEELDGGPVVLQAALQVK
PGDDIESLTQRVHVAEHQIYPLAMRWFAEGRLRLAEQGAMLDGVTLPVSGYQIKI

Sequences:

>Translated_215_residues
MGTTCNVVVLISGSGSNLQALIDSQHEGNPARIRAVIANRVDAFGLTRAKGAGIPTAVLDHKAFDGREAFDAALMELIDA
HAPDLVILAGFMRILSPGFVRHYHGRLLNIHPSLLPKYKGLDTHRRALEAGDAEHGCSVHFVTEELDGGPVVLQAALQVK
PGDDIESLTQRVHVAEHQIYPLAMRWFAEGRLRLAEQGAMLDGVTLPVSGYQIKI
>Mature_214_residues
GTTCNVVVLISGSGSNLQALIDSQHEGNPARIRAVIANRVDAFGLTRAKGAGIPTAVLDHKAFDGREAFDAALMELIDAH
APDLVILAGFMRILSPGFVRHYHGRLLNIHPSLLPKYKGLDTHRRALEAGDAEHGCSVHFVTEELDGGPVVLQAALQVKP
GDDIESLTQRVHVAEHQIYPLAMRWFAEGRLRLAEQGAMLDGVTLPVSGYQIKI

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=195, Percent_Identity=50.2564102564103, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI209869995, Length=195, Percent_Identity=50.2564102564103, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI209869993, Length=195, Percent_Identity=50.2564102564103, Blast_Score=199, Evalue=2e-51,
Organism=Escherichia coli, GI1788846, Length=207, Percent_Identity=62.3188405797101, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1787483, Length=196, Percent_Identity=30.1020408163265, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=40.2234636871508, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6320616, Length=196, Percent_Identity=37.2448979591837, Blast_Score=107, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=52.9411764705882, Blast_Score=194, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23250; Mature: 23119

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTCNVVVLISGSGSNLQALIDSQHEGNPARIRAVIANRVDAFGLTRAKGAGIPTAVLD
CCCCEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHC
HKAFDGREAFDAALMELIDAHAPDLVILAGFMRILSPGFVRHYHGRLLNIHPSLLPKYKG
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHCCCEEEECHHHCCCCCC
LDTHRRALEAGDAEHGCSVHFVTEELDGGPVVLQAALQVKPGDDIESLTQRVHVAEHQIY
CCHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
PLAMRWFAEGRLRLAEQGAMLDGVTLPVSGYQIKI
HHHHHHHHCCCEEEECCCCEECCEEECCCCEEEEC
>Mature Secondary Structure 
GTTCNVVVLISGSGSNLQALIDSQHEGNPARIRAVIANRVDAFGLTRAKGAGIPTAVLD
CCCEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHC
HKAFDGREAFDAALMELIDAHAPDLVILAGFMRILSPGFVRHYHGRLLNIHPSLLPKYKG
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHCCCEEEECHHHCCCCCC
LDTHRRALEAGDAEHGCSVHFVTEELDGGPVVLQAALQVKPGDDIESLTQRVHVAEHQIY
CCHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
PLAMRWFAEGRLRLAEQGAMLDGVTLPVSGYQIKI
HHHHHHHHCCCEEEECCCCEECCEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]