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The map label for this gene is folE [H]

Identifier: 146282677

GI number: 146282677

Start: 2547041

End: 2547586

Strand: Direct

Name: folE [H]

Synonym: PST_2333

Alternate gene names: 146282677

Gene position: 2547041-2547586 (Clockwise)

Preceding gene: 146282676

Following gene: 146282680

Centisome position: 55.77

GC content: 59.71

Gene sequence:

>546_bases
ATGTCCCTGGAACAACACTACACCGCGATCCTTGGCCAACTTGGCGAGGACGTTAACCGCGAGGGTCTGCTCGACACACC
CAAACGCGCCGCCAAAGCCATGCAGTACCTGTGCAAGGGCTACCAGCAGACCCTCGAAGAGGTGACGAACGGCGCGCTAT
TCAGCTCCGACAACAGCGAGATGGTGCTGGTCAAGAACATCGAGCTGTACTCGCTCTGCGAACACCACATGCTGCCTTTC
ATCGGCAAGGCGCACGTCGCCTACCTGCCCAACGGCAAGGTGCTTGGCCTGTCGAAGGTCGCCCGGATCGTCGACATGTA
TGCGCGCCGACTGCAGATTCAGGAAAACCTGACCCGCGAGATCGCCGAAGCGATGCAGCAAGTCACCGGAGCCTCCGGTG
TAGCCGTGGTCATCGAAGCCCAGCACATGTGCATGATGATGCGCGGTGTCGAGAAGCAGAATTCTTCCATGGTCACATCG
GTGATGCTGGGCCAGTTCCGCGACAACGCCGCGACTCGTAGCGAGTTTCTCGGGCTGGTGAAGTAG

Upstream 100 bases:

>100_bases
GACTGCTCGGCTCACGCCAGTCGCTCGACAGGCTTGCCAGCAGAGCGGCAGCCCCCTACCCTTCGCGTTCGCGACCCTAT
GAAGCCTGACAGGTAGCTCA

Downstream 100 bases:

>100_bases
CACAGAGAAGACAGGGTCTTCACGGCCCTGCCTTTTTCACTTCTCAATCGTACATGCCTACATGCCGCGGATGGCTCGCA
ACGCGCTGCAGCCACTGACG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I 2; GTP-CH-I 2 [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSLEQHYTAILGQLGEDVNREGLLDTPKRAAKAMQYLCKGYQQTLEEVTNGALFSSDNSEMVLVKNIELYSLCEHHMLPF
IGKAHVAYLPNGKVLGLSKVARIVDMYARRLQIQENLTREIAEAMQQVTGASGVAVVIEAQHMCMMMRGVEKQNSSMVTS
VMLGQFRDNAATRSEFLGLVK

Sequences:

>Translated_181_residues
MSLEQHYTAILGQLGEDVNREGLLDTPKRAAKAMQYLCKGYQQTLEEVTNGALFSSDNSEMVLVKNIELYSLCEHHMLPF
IGKAHVAYLPNGKVLGLSKVARIVDMYARRLQIQENLTREIAEAMQQVTGASGVAVVIEAQHMCMMMRGVEKQNSSMVTS
VMLGQFRDNAATRSEFLGLVK
>Mature_180_residues
SLEQHYTAILGQLGEDVNREGLLDTPKRAAKAMQYLCKGYQQTLEEVTNGALFSSDNSEMVLVKNIELYSLCEHHMLPFI
GKAHVAYLPNGKVLGLSKVARIVDMYARRLQIQENLTREIAEAMQQVTGASGVAVVIEAQHMCMMMRGVEKQNSSMVTSV
MLGQFRDNAATRSEFLGLVK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=175, Percent_Identity=57.7142857142857, Blast_Score=233, Evalue=6e-62,
Organism=Homo sapiens, GI4503949, Length=175, Percent_Identity=57.7142857142857, Blast_Score=233, Evalue=6e-62,
Organism=Homo sapiens, GI66932972, Length=134, Percent_Identity=56.7164179104478, Blast_Score=178, Evalue=2e-45,
Organism=Homo sapiens, GI66932970, Length=134, Percent_Identity=56.7164179104478, Blast_Score=178, Evalue=3e-45,
Organism=Escherichia coli, GI1788476, Length=187, Percent_Identity=37.4331550802139, Blast_Score=115, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=174, Percent_Identity=61.4942528735632, Blast_Score=238, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6321706, Length=173, Percent_Identity=59.5375722543353, Blast_Score=219, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24656791, Length=174, Percent_Identity=59.7701149425287, Blast_Score=216, Evalue=8e-57,
Organism=Drosophila melanogaster, GI24656796, Length=174, Percent_Identity=59.7701149425287, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24656782, Length=174, Percent_Identity=59.7701149425287, Blast_Score=214, Evalue=2e-56,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 20127; Mature: 19996

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEQHYTAILGQLGEDVNREGLLDTPKRAAKAMQYLCKGYQQTLEEVTNGALFSSDNSE
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
MVLVKNIELYSLCEHHMLPFIGKAHVAYLPNGKVLGLSKVARIVDMYARRLQIQENLTRE
EEEEECCHHHHHHHHHHCCHHCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
IAEAMQQVTGASGVAVVIEAQHMCMMMRGVEKQNSSMVTSVMLGQFRDNAATRSEFLGLV
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCC
K
C
>Mature Secondary Structure 
SLEQHYTAILGQLGEDVNREGLLDTPKRAAKAMQYLCKGYQQTLEEVTNGALFSSDNSE
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
MVLVKNIELYSLCEHHMLPFIGKAHVAYLPNGKVLGLSKVARIVDMYARRLQIQENLTRE
EEEEECCHHHHHHHHHHCCHHCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
IAEAMQQVTGASGVAVVIEAQHMCMMMRGVEKQNSSMVTSVMLGQFRDNAATRSEFLGLV
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]