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The map label for this gene is aat

Identifier: 146282643

GI number: 146282643

Start: 2505959

End: 2506639

Strand: Direct

Name: aat

Synonym: PST_2295

Alternate gene names: 146282643

Gene position: 2505959-2506639 (Clockwise)

Preceding gene: 146282642

Following gene: 146282644

Centisome position: 54.87

GC content: 65.49

Gene sequence:

>681_bases
ATGCTGACCTGGCTAAAGCGCGACGACCTCAGCTTTCCTCCCCTCGAAACGGCTCTGCGCGAACCCAACGGCCTGCTTGC
CGCTGGCGGCGACTTGCGGCCCGAGCGACTCCTGGCTGCCTACCGCCACGGCTGTTTCCCCTGGTACCAGGAAGGCCAAC
CCCTGTTGTGGTGGTCGCCCGATCCGCGCACGGTGCTCTTTCCTGACGAGCTGCATGTATCGCGCAGTCTGCGCAAGCGC
ATGCGCCACGGCGACTACCGGGTGACCTTCGACAAGGCCTTTGCCGAGGTCATCCAGGGATGCGCCGGTCCGCGGAGCTA
CGCCGACGGCACCTGGATCACGACACCGATGCAAGACGCTTATGTCAGGCTGCACGAGATGGGCGTGGCTCACTCAGTGG
AAGTCTGGCAGCAAGGGCAACTCGTCGGCGGACTCTACGGCCTCGCGATGGGTGAGCTATTTTTCGGCGAGTCGATGTTC
AGCCGCGCCACCGATGCGTCCAAGGTCGGCTTCGTCACGCTGGTGGAACGGCTGCGCGAATGGGGCTTCGCATTGATCGA
CTGCCAGATGCCGACGCGCCACCTGGAGAGCTTCGGCGCTCGCAGCATCCCACGCGCGGCGTTCGCCGAGGCACTCGCAA
TGCATCTGGATCGCCCCAGCGCAGCCGACTGGCGCGCCTAG

Upstream 100 bases:

>100_bases
GGCAGGCGCCGGCTGCATGGCGGCACTGGATGCCGAGAAGTACCTGGACAATTGATCCCAGGCGGGCTTCGGCCCGCCCT
CCCCTACCCTGCGTGCCGAC

Downstream 100 bases:

>100_bases
TCGACCGCGGTAAGCTGGCCTACACTGGGTTCATGATTTCTGAGAGTTGACCATGACTTCACTGGCTCGCCTCAAGTTCT
ACGCCACCCAGCCCCACGCT

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MLTWLKRDDLSFPPLETALREPNGLLAAGGDLRPERLLAAYRHGCFPWYQEGQPLLWWSPDPRTVLFPDELHVSRSLRKR
MRHGDYRVTFDKAFAEVIQGCAGPRSYADGTWITTPMQDAYVRLHEMGVAHSVEVWQQGQLVGGLYGLAMGELFFGESMF
SRATDASKVGFVTLVERLREWGFALIDCQMPTRHLESFGARSIPRAAFAEALAMHLDRPSAADWRA

Sequences:

>Translated_226_residues
MLTWLKRDDLSFPPLETALREPNGLLAAGGDLRPERLLAAYRHGCFPWYQEGQPLLWWSPDPRTVLFPDELHVSRSLRKR
MRHGDYRVTFDKAFAEVIQGCAGPRSYADGTWITTPMQDAYVRLHEMGVAHSVEVWQQGQLVGGLYGLAMGELFFGESMF
SRATDASKVGFVTLVERLREWGFALIDCQMPTRHLESFGARSIPRAAFAEALAMHLDRPSAADWRA
>Mature_226_residues
MLTWLKRDDLSFPPLETALREPNGLLAAGGDLRPERLLAAYRHGCFPWYQEGQPLLWWSPDPRTVLFPDELHVSRSLRKR
MRHGDYRVTFDKAFAEVIQGCAGPRSYADGTWITTPMQDAYVRLHEMGVAHSVEVWQQGQLVGGLYGLAMGELFFGESMF
SRATDASKVGFVTLVERLREWGFALIDCQMPTRHLESFGARSIPRAAFAEALAMHLDRPSAADWRA

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=224, Percent_Identity=52.2321428571429, Blast_Score=229, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_PSEU5 (A4VLV3)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001172796.1
- ProteinModelPortal:   A4VLV3
- SMR:   A4VLV3
- STRING:   A4VLV3
- GeneID:   5098375
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_2295
- eggNOG:   COG2360
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- PhylomeDB:   A4VLV3
- ProtClustDB:   PRK00301
- BioCyc:   PSTU379731:PST_2295-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 25598; Mature: 25598

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTWLKRDDLSFPPLETALREPNGLLAAGGDLRPERLLAAYRHGCFPWYQEGQPLLWWSP
CCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHCCCEEEEECC
DPRTVLFPDELHVSRSLRKRMRHGDYRVTFDKAFAEVIQGCAGPRSYADGTWITTPMQDA
CCCEEECCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCEEECCHHHH
YVRLHEMGVAHSVEVWQQGQLVGGLYGLAMGELFFGESMFSRATDASKVGFVTLVERLRE
HHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
WGFALIDCQMPTRHLESFGARSIPRAAFAEALAMHLDRPSAADWRA
CCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLTWLKRDDLSFPPLETALREPNGLLAAGGDLRPERLLAAYRHGCFPWYQEGQPLLWWSP
CCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHCCCEEEEECC
DPRTVLFPDELHVSRSLRKRMRHGDYRVTFDKAFAEVIQGCAGPRSYADGTWITTPMQDA
CCCEEECCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCEEECCHHHH
YVRLHEMGVAHSVEVWQQGQLVGGLYGLAMGELFFGESMFSRATDASKVGFVTLVERLRE
HHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
WGFALIDCQMPTRHLESFGARSIPRAAFAEALAMHLDRPSAADWRA
CCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA