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The map label for this gene is fabI [H]

Identifier: 146282610

GI number: 146282610

Start: 2468553

End: 2469347

Strand: Direct

Name: fabI [H]

Synonym: PST_2262

Alternate gene names: 146282610

Gene position: 2468553-2469347 (Clockwise)

Preceding gene: 146282609

Following gene: 146282612

Centisome position: 54.05

GC content: 65.28

Gene sequence:

>795_bases
ATGGGTTTTCTCACCGGTAAGCGCGTACTGATCGTCGGCGTTGCCAGCAAACTGTCGATTGCCTCGGGCATCGCCGCCGC
CATGCATCGCGAAGGTGCTGAACTGGCTTTCACCTACCAGAACGACAAGCTCAAGGGCCGCGTCGAGGAATTCGCCGCCG
GCTGGGGCTCGAGCCCGGAGCTGTGCTTCCCCTGCGACGTCGCCAATGACGAAGACATCGCCCGCGTGTTCGAGGAGCTG
GGCAAGAAGTGGGACGGCCTGGACTGCATCGTTCACTCCGTCGGCTTCGCCCCGGGCGACCAGCTGAACGGCGACTTCAC
CGAAGTCACCACCCGCGAAGGCTTCAAGATCGCCCACGACATCAGCGCCTACAGCTTCGTCGCCCTGGCCAAGGCCGGTC
GCGAGATGATGAAGGGCCGCAACGGCAGCCTGCTCACGCTCTCCTACCTCGGCGCCGAGCGCACCATGCCGAACTACAAT
GTGATGGGCATGGCCAAGGCCAGCCTGGAAGCCGGTGTACGCTACCTGGCTGGCAGCCTCGGCCCGGAAGGCACCCGCGT
CAACTGCATCTCCGCCGGCCCGATCCGCACCTTGGCAGCCTCCGGCATCGCCAGCTTCCGCAAGATGCTCGCCGCCAACG
AGAAGCAGACCCCGCTGCGCCGCAACGTCACCATCGAGGAAGTCGGCAACGCCGGTGCCTTCCTCTGCTCGGACCTGGCC
TCGGGCATCAGCGGCGAGATCCTCTACGTGGATGGCGGCTTCAACACCACCGCCATGGGCTCGCTGGAAGACTGA

Upstream 100 bases:

>100_bases
GAGATATTCGCGGCGCCGCAACATCCGTATACACAGCAGCTGCTGGAATCCGCCTTCATGGCGCCCGGCGCTGCCGAACA
ACCAGAAGAGGGACAAGCAC

Downstream 100 bases:

>100_bases
TCCTGCGCCCGCCTACGTAAAGCACCGAACCCCGCCTACTGGCGGGGTTCGTGTTTCTAGGGCGGCATCAGCCGGCCGTG
ACCGTCATGCCGCCATCGGC

Product: enoyl-(acyl-carrier-protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MGFLTGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAAGWGSSPELCFPCDVANDEDIARVFEEL
GKKWDGLDCIVHSVGFAPGDQLNGDFTEVTTREGFKIAHDISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYN
VMGMAKASLEAGVRYLAGSLGPEGTRVNCISAGPIRTLAASGIASFRKMLAANEKQTPLRRNVTIEEVGNAGAFLCSDLA
SGISGEILYVDGGFNTTAMGSLED

Sequences:

>Translated_264_residues
MGFLTGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAAGWGSSPELCFPCDVANDEDIARVFEEL
GKKWDGLDCIVHSVGFAPGDQLNGDFTEVTTREGFKIAHDISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYN
VMGMAKASLEAGVRYLAGSLGPEGTRVNCISAGPIRTLAASGIASFRKMLAANEKQTPLRRNVTIEEVGNAGAFLCSDLA
SGISGEILYVDGGFNTTAMGSLED
>Mature_263_residues
GFLTGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAAGWGSSPELCFPCDVANDEDIARVFEELG
KKWDGLDCIVHSVGFAPGDQLNGDFTEVTTREGFKIAHDISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYNV
MGMAKASLEAGVRYLAGSLGPEGTRVNCISAGPIRTLAASGIASFRKMLAANEKQTPLRRNVTIEEVGNAGAFLCSDLAS
GISGEILYVDGGFNTTAMGSLED

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503301, Length=233, Percent_Identity=28.755364806867, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI32483357, Length=197, Percent_Identity=30.9644670050761, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI7705925, Length=257, Percent_Identity=27.2373540856031, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI59889578, Length=197, Percent_Identity=28.9340101522843, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787545, Length=263, Percent_Identity=70.722433460076, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1787905, Length=257, Percent_Identity=28.7937743190661, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI2367365, Length=266, Percent_Identity=30.8270676691729, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082100, Length=262, Percent_Identity=28.2442748091603, Blast_Score=62, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24644339, Length=253, Percent_Identity=28.0632411067194, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24644337, Length=200, Percent_Identity=31, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357041, Length=251, Percent_Identity=27.8884462151394, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27964; Mature: 27833

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLTGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAAGWGSSPE
CCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCC
LCFPCDVANDEDIARVFEELGKKWDGLDCIVHSVGFAPGDQLNGDFTEVTTREGFKIAHD
EEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHEEECCCCCEEEHH
ISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYNVMGMAKASLEAGVRYLAGSL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHCC
GPEGTRVNCISAGPIRTLAASGIASFRKMLAANEKQTPLRRNVTIEEVGNAGAFLCSDLA
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCHHHHHHHHH
SGISGEILYVDGGFNTTAMGSLED
CCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
GFLTGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAAGWGSSPE
CCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCC
LCFPCDVANDEDIARVFEELGKKWDGLDCIVHSVGFAPGDQLNGDFTEVTTREGFKIAHD
EEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHEEECCCCCEEEHH
ISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYNVMGMAKASLEAGVRYLAGSL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHCC
GPEGTRVNCISAGPIRTLAASGIASFRKMLAANEKQTPLRRNVTIEEVGNAGAFLCSDLA
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCCHHHHHHHHH
SGISGEILYVDGGFNTTAMGSLED
CCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10464225; 10984043 [H]