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The map label for this gene is acsA [H]

Identifier: 145301080

GI number: 145301080

Start: 4543600

End: 4545537

Strand: Direct

Name: acsA [H]

Synonym: ASA_4249

Alternate gene names: 145301080

Gene position: 4543600-4545537 (Clockwise)

Preceding gene: 145301075

Following gene: 145301081

Centisome position: 96.62

GC content: 63.21

Gene sequence:

>1938_bases
ATGCACAATCTTTGCCTGTGGCAACCGGATCAGGACAGGATTCAACAATCCAATCTCCAGCAGTTTATGGGCGAGGTCAA
CCGCTTCCACGGCCTGCAACTGCACAGCTATGCCCAGCTCTATCAGTGGTCGGTGGAAAAGACCACTCGCTTCTGGCCTC
TGGTGTGGCAGCACTGTGGCGTCAAGGGCGAGCTCGGCGACATAGTGGCAGAGAACCGCCAGGATATGCAGCGCACCCGC
TGGTTCCCCGATAGCCGACTCAACTTCGCCGAAAACCTGCTGCGCCGCCAGGATGAGAGTCCGGCCATTATCTCCCGCAT
CGAAGGCAGCCCAAGCCGCACCCTGAGCTGGCGTGAACTGGCGGATCAGGTGGCCAGGCTGGCCCAGTGGCTGCGCAGCC
AGGGCATAGGCAGTGGCGACGTGGTGGCGGCCTACCTGCCCAATATCCCGGAAACCGTGGTGGCCATGCTGGCAACCACC
AGCCTGGGCGCTATCTGGACCTCCACCAGCCCCGACTTTGGCGAGGCCAGCGTGGTGGAGCGCTTCGGCCAGACCCGGCC
GCGGGTGCTGTTTGCGGTAGACGGCTACCGCTACAACGGCAAGGCCATCGACATTCAGGACAAAGTGGCGGGCGTGGTCG
GCCAGATCGACAGCATAGAGCAGACTGTGCTGATCCCCCTGCTCGGCAATCCCCTGCGGCTTGGTCACGACTGGCAGCAC
GTGCTGGCCAGCCAGCCGGATGCCGTCCTTCGCTTCGAGCCCATGGGCTTCAACGATCCGCTCTACATCCTCTATTCGTC
CGGCACCACTGGCAAGCCCAAGTGCATAGTGCACGGCATAGGTGGCACCCTGCTGCAACATCTGAAAGAGCACCAGCTGC
ACTGCGACATCAAGCCGGGGGAGCGGGTGTTCTACTTCACCACCTGCGGCTGGATGATGTGGAACTGGCTGGTGAGCGCG
CTCGCCTCCGGCGCGACTCTGGTGCTCTATGATGGCTCCCCCTTCTATCCGGACGGCAATGTGCTGTGGGATCTCGCTCG
CGATGAGCAGGTTACCCTGTTCGGCACCTCGGCCAAGTACCTGGATGCCCTGCACAAGCAGGGCTATGCCCCCATCAAGA
CCCACGAGTTGCCGCAGCTGAGGCTCATCTGCAGCACGGGCTCCGTGCTGTCGCCGGAGGGGTTTGACTATGTCTATCAG
GGGATCAAACAGGATGTGCAGCTCAGCTCCATCTCCGGCGGCACCGACATCTGCTCCTGCTTCGTCATCGGCAATCCCAT
CAGCCCCGTCTATCGCGGCGAGAGCCAGGGACGCGGGCTTGGCCTCGCGGTGCAGGTGTTCAACGAGGCGGGCCAGCCGG
TGCAGGGCGAGAAGGGGGAACTGGTCTGCACCAAGCCCTTCCCGGCCCAACCCATCGGCTTTTGGGGTGACGAGAGCGGG
GACAAGTACCACGCCGCCTACTTCGAGCGATTCGACAACATCTGGTGCCATGGCGACTGGATCGAGCTGACCGACACCGG
CGGCATCCTCTTCTACGGCCGCTCCGATGCCACTCTCAATCCGGGGGGCGTGCGCATCGGCACCAGCGAGATATACCGCT
ACGTGGAGCAGTTGGACGAGGTGGAAGAGAGCATCGTCATCGGCCAGCAGTGGCAGCAGGACGAGCGGGTGGTGTTGTTC
GTCAAGCTCAAGCCCGGCCTCAAGCTGGACGATCCCCTGCGCGAGCGCATCCGCCAGCAAGTGAGGCAGCACTGCACGGC
CCGCCACGTACCCGCCCGTATCCTGCAGGTGGATGCGATCCCCCGCACCAAATCCGGCAAGATAGTGGAGCTGGCAGTGC
GGGAAGTGGTGCACAACAGACCCGTCAACAACACCCATGCCCTCGCCGATCCCGAGGTGCTGAGCCAGTATCGCGACCGG
CCCGAACTGGCCTGTTGA

Upstream 100 bases:

>100_bases
ATACGTTACAAAGCTTTTGTTATGAAACAAAAACCAACCATTAACAAATAAAAAACAATTCAATACACTGCCGGTAGATA
CATGCAGTCAGGGACTACTC

Downstream 100 bases:

>100_bases
TACAAAACTCATATCCATTGGAGGCGCCGCAAGGCGCCTCTCTTTTGCCCGTTTTGTGATGAGGAGCTTTTACTCGAGAA
CTGTTAAACAAATGTTTTAA

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MHNLCLWQPDQDRIQQSNLQQFMGEVNRFHGLQLHSYAQLYQWSVEKTTRFWPLVWQHCGVKGELGDIVAENRQDMQRTR
WFPDSRLNFAENLLRRQDESPAIISRIEGSPSRTLSWRELADQVARLAQWLRSQGIGSGDVVAAYLPNIPETVVAMLATT
SLGAIWTSTSPDFGEASVVERFGQTRPRVLFAVDGYRYNGKAIDIQDKVAGVVGQIDSIEQTVLIPLLGNPLRLGHDWQH
VLASQPDAVLRFEPMGFNDPLYILYSSGTTGKPKCIVHGIGGTLLQHLKEHQLHCDIKPGERVFYFTTCGWMMWNWLVSA
LASGATLVLYDGSPFYPDGNVLWDLARDEQVTLFGTSAKYLDALHKQGYAPIKTHELPQLRLICSTGSVLSPEGFDYVYQ
GIKQDVQLSSISGGTDICSCFVIGNPISPVYRGESQGRGLGLAVQVFNEAGQPVQGEKGELVCTKPFPAQPIGFWGDESG
DKYHAAYFERFDNIWCHGDWIELTDTGGILFYGRSDATLNPGGVRIGTSEIYRYVEQLDEVEESIVIGQQWQQDERVVLF
VKLKPGLKLDDPLRERIRQQVRQHCTARHVPARILQVDAIPRTKSGKIVELAVREVVHNRPVNNTHALADPEVLSQYRDR
PELAC

Sequences:

>Translated_645_residues
MHNLCLWQPDQDRIQQSNLQQFMGEVNRFHGLQLHSYAQLYQWSVEKTTRFWPLVWQHCGVKGELGDIVAENRQDMQRTR
WFPDSRLNFAENLLRRQDESPAIISRIEGSPSRTLSWRELADQVARLAQWLRSQGIGSGDVVAAYLPNIPETVVAMLATT
SLGAIWTSTSPDFGEASVVERFGQTRPRVLFAVDGYRYNGKAIDIQDKVAGVVGQIDSIEQTVLIPLLGNPLRLGHDWQH
VLASQPDAVLRFEPMGFNDPLYILYSSGTTGKPKCIVHGIGGTLLQHLKEHQLHCDIKPGERVFYFTTCGWMMWNWLVSA
LASGATLVLYDGSPFYPDGNVLWDLARDEQVTLFGTSAKYLDALHKQGYAPIKTHELPQLRLICSTGSVLSPEGFDYVYQ
GIKQDVQLSSISGGTDICSCFVIGNPISPVYRGESQGRGLGLAVQVFNEAGQPVQGEKGELVCTKPFPAQPIGFWGDESG
DKYHAAYFERFDNIWCHGDWIELTDTGGILFYGRSDATLNPGGVRIGTSEIYRYVEQLDEVEESIVIGQQWQQDERVVLF
VKLKPGLKLDDPLRERIRQQVRQHCTARHVPARILQVDAIPRTKSGKIVELAVREVVHNRPVNNTHALADPEVLSQYRDR
PELAC
>Mature_645_residues
MHNLCLWQPDQDRIQQSNLQQFMGEVNRFHGLQLHSYAQLYQWSVEKTTRFWPLVWQHCGVKGELGDIVAENRQDMQRTR
WFPDSRLNFAENLLRRQDESPAIISRIEGSPSRTLSWRELADQVARLAQWLRSQGIGSGDVVAAYLPNIPETVVAMLATT
SLGAIWTSTSPDFGEASVVERFGQTRPRVLFAVDGYRYNGKAIDIQDKVAGVVGQIDSIEQTVLIPLLGNPLRLGHDWQH
VLASQPDAVLRFEPMGFNDPLYILYSSGTTGKPKCIVHGIGGTLLQHLKEHQLHCDIKPGERVFYFTTCGWMMWNWLVSA
LASGATLVLYDGSPFYPDGNVLWDLARDEQVTLFGTSAKYLDALHKQGYAPIKTHELPQLRLICSTGSVLSPEGFDYVYQ
GIKQDVQLSSISGGTDICSCFVIGNPISPVYRGESQGRGLGLAVQVFNEAGQPVQGEKGELVCTKPFPAQPIGFWGDESG
DKYHAAYFERFDNIWCHGDWIELTDTGGILFYGRSDATLNPGGVRIGTSEIYRYVEQLDEVEESIVIGQQWQQDERVVLF
VKLKPGLKLDDPLRERIRQQVRQHCTARHVPARILQVDAIPRTKSGKIVELAVREVVHNRPVNNTHALADPEVLSQYRDR
PELAC

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=654, Percent_Identity=44.8012232415902, Blast_Score=563, Evalue=1e-160,
Organism=Homo sapiens, GI8923896, Length=661, Percent_Identity=27.5340393343419, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI28416953, Length=577, Percent_Identity=26.6897746967071, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI253970489, Length=674, Percent_Identity=27.1513353115727, Blast_Score=192, Evalue=9e-49,
Organism=Homo sapiens, GI13375727, Length=596, Percent_Identity=25.503355704698, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI115511026, Length=513, Percent_Identity=23.3918128654971, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI38505220, Length=506, Percent_Identity=21.9367588932806, Blast_Score=88, Evalue=3e-17,
Organism=Escherichia coli, GI1790505, Length=627, Percent_Identity=27.591706539075, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI1786529, Length=645, Percent_Identity=26.5116279069767, Blast_Score=164, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17569507, Length=630, Percent_Identity=37.4603174603175, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71982997, Length=650, Percent_Identity=25.5384615384615, Blast_Score=179, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI71983001, Length=650, Percent_Identity=25.5384615384615, Blast_Score=178, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6323182, Length=641, Percent_Identity=26.8330733229329, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6319264, Length=677, Percent_Identity=24.6676514032496, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24667955, Length=647, Percent_Identity=26.1205564142195, Blast_Score=219, Evalue=4e-57,
Organism=Drosophila melanogaster, GI62472339, Length=556, Percent_Identity=27.3381294964029, Blast_Score=213, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24667959, Length=522, Percent_Identity=26.2452107279693, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI21355225, Length=649, Percent_Identity=24.8073959938367, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 72496; Mature: 72496

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNLCLWQPDQDRIQQSNLQQFMGEVNRFHGLQLHSYAQLYQWSVEKTTRFWPLVWQHCG
CCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VKGELGDIVAENRQDMQRTRWFPDSRLNFAENLLRRQDESPAIISRIEGSPSRTLSWREL
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHH
ADQVARLAQWLRSQGIGSGDVVAAYLPNIPETVVAMLATTSLGAIWTSTSPDFGEASVVE
HHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHH
RFGQTRPRVLFAVDGYRYNGKAIDIQDKVAGVVGQIDSIEQTVLIPLLGNPLRLGHDWQH
HHCCCCCEEEEEECCEEECCEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHH
VLASQPDAVLRFEPMGFNDPLYILYSSGTTGKPKCIVHGIGGTLLQHLKEHQLHCDIKPG
HHHCCCCCEEEEECCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHCCEEEECCCC
ERVFYFTTCGWMMWNWLVSALASGATLVLYDGSPFYPDGNVLWDLARDEQVTLFGTSAKY
CEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHH
LDALHKQGYAPIKTHELPQLRLICSTGSVLSPEGFDYVYQGIKQDVQLSSISGGTDICSC
HHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEECCCCCCCEEEE
FVIGNPISPVYRGESQGRGLGLAVQVFNEAGQPVQGEKGELVCTKPFPAQPIGFWGDESG
EEECCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCC
DKYHAAYFERFDNIWCHGDWIELTDTGGILFYGRSDATLNPGGVRIGTSEIYRYVEQLDE
CCHHHHHHHHHCCCEECCCEEEEECCCCEEEECCCCCEECCCCEEECHHHHHHHHHHHHH
VEESIVIGQQWQQDERVVLFVKLKPGLKLDDPLRERIRQQVRQHCTARHVPARILQVDAI
HHHHEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEECC
PRTKSGKIVELAVREVVHNRPVNNTHALADPEVLSQYRDRPELAC
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHNLCLWQPDQDRIQQSNLQQFMGEVNRFHGLQLHSYAQLYQWSVEKTTRFWPLVWQHCG
CCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VKGELGDIVAENRQDMQRTRWFPDSRLNFAENLLRRQDESPAIISRIEGSPSRTLSWREL
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCHHHH
ADQVARLAQWLRSQGIGSGDVVAAYLPNIPETVVAMLATTSLGAIWTSTSPDFGEASVVE
HHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHH
RFGQTRPRVLFAVDGYRYNGKAIDIQDKVAGVVGQIDSIEQTVLIPLLGNPLRLGHDWQH
HHCCCCCEEEEEECCEEECCEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHH
VLASQPDAVLRFEPMGFNDPLYILYSSGTTGKPKCIVHGIGGTLLQHLKEHQLHCDIKPG
HHHCCCCCEEEEECCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHCCEEEECCCC
ERVFYFTTCGWMMWNWLVSALASGATLVLYDGSPFYPDGNVLWDLARDEQVTLFGTSAKY
CEEEEEECHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHH
LDALHKQGYAPIKTHELPQLRLICSTGSVLSPEGFDYVYQGIKQDVQLSSISGGTDICSC
HHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEECCCCCCCEEEE
FVIGNPISPVYRGESQGRGLGLAVQVFNEAGQPVQGEKGELVCTKPFPAQPIGFWGDESG
EEECCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCC
DKYHAAYFERFDNIWCHGDWIELTDTGGILFYGRSDATLNPGGVRIGTSEIYRYVEQLDE
CCHHHHHHHHHCCCEECCCEEEEECCCCEEEECCCCCEECCCCEEECHHHHHHHHHHHHH
VEESIVIGQQWQQDERVVLFVKLKPGLKLDDPLRERIRQQVRQHCTARHVPARILQVDAI
HHHHEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEECC
PRTKSGKIVELAVREVVHNRPVNNTHALADPEVLSQYRDRPELAC
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]