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The map label for this gene is glnA [H]

Identifier: 145300967

GI number: 145300967

Start: 4412314

End: 4413723

Strand: Direct

Name: glnA [H]

Synonym: ASA_4120

Alternate gene names: 145300967

Gene position: 4412314-4413723 (Clockwise)

Preceding gene: 145300964

Following gene: 145300968

Centisome position: 93.83

GC content: 56.67

Gene sequence:

>1410_bases
ATGTCAGCCCAGAACGTTTTGGCCCTGATCCAGGAACACGAAGTCAAGTTTGTTGACCTGCGCTTTACCGACACCAAGGG
TAAAGAGCAGCACGTATCTATCCCTTCCCACCAGGTCAACGAAGATTTCTTCGAAGACGGCAAGATGTTCGATGGCTCTT
CCATCGGCGGCTGGAAAGGTATCAACGAGTCTGACATGCTGCTGATGCCGGATGCGGCCTCCGCCAAGCTGGACCCGTTT
ACCGAAGAGACCACCCTGAACATCGTTTGTGATGTGCTGGAACCGGCCACCATGCAAGGTTATGACCGCGACCCGCGCTC
CATCTCCAAGCGTGCCGAAGATTTCCTCAAAGCCAGCGGCATCGCTGACACAGTGCTGTTCGGGCCTGAGCCGGAATTCT
TCATGTTCGACAACATCACCTTCTCGAACACCATGGGCCACAGCTTCGTGAAAATCGAATCTGAAGAAGCCGCCTGGAAC
TCGGGTACCGAGTATGAGCACGGCAACAAGGGCCACCGCCCGTTCGTGAAAGGTGGTTACTTCCCGGTTGCACCGGTTGA
CTCTTCCCAGGACATCCGCGCTGCCATGTGTCTAGTGCTGGAAGAGATGGGCCAGGTCGTTGAAGCGCACCACCATGAAG
TGGCGACCGCCGGTCAGAACGAGATCGCGACCCGTTTCAACACTCTCACCCTGAAAGCGGACGAAGTACAGGTTCTGAAG
TACGTGATCCATAACGTGGCCCACGCTTACAACAAGACTGTCACCTTCATGCCGAAACCCATGTTCGGTGACAACGGCTC
CGGCATGCACTGCCACCAGTCCCTAGCCAAGAACGGTGTCAACCTGTTCGCTGGCGACCTGTACGGTGGTCTGTCCGAGA
TGGCACTGCACTACATCGGCGGTATCATCAAGCACGCCAAGGCAATCAATGCCTTCGCCAACCCGACCACCAACTCCTAC
AAGCGTCTGGTTCCGGGTTATGAAGCACCTGTCATGCTGGCTTACTCCGCCCGCAACCGCTCTGCTTCCATCCGTATCCC
GGTAGTACCGAGCCCGAAAGCTCGTCGTATCGAAGTACGTTTCCCGGATCCGGCAGCCAACCCGTACCTGGCTTTTGCAG
CTCAGCTGATGGCCGGTCTGGACGGTATCATCAACAAGATCCATCCGGGCGATGCCATGGACAAGAACTTGTATGACCTG
CCGCCGGAAGAAGCCGCAGAAGTGCCGACCGTTGCCGGCTCTCTGGACGAAGCACTGGCCGCACTGGACAGCGATCGCGA
GTTCCTGACCCGCGGCGGCGTGTTCAGCGATGACTTCATCAATTCCTATCTGGAACTGAAACAGCATGATGTTGACCGTG
TGCGCATGACCCCGCACCCGCTGGAGTTTGAACTGTACTACTCCGTTTAA

Upstream 100 bases:

>100_bases
CGCCCTGAAATCAAGCTGTCACAAACTTGGCACGATACTGGCTTATGTGAATGTGATGACGCATTCACCCTAAGAGTTTT
AACACCGGAGGTTACTTAGC

Downstream 100 bases:

>100_bases
TCCGGACCACAGCTCGGAACTTGACCAAACCCGCCAACAGGCGGGTTTTTTCTCTGATAGGATTAAGCAAAATCCCGTCA
AGGAGAGATGGATGAAAAAC

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSAQNVLALIQEHEVKFVDLRFTDTKGKEQHVSIPSHQVNEDFFEDGKMFDGSSIGGWKGINESDMLLMPDAASAKLDPF
TEETTLNIVCDVLEPATMQGYDRDPRSISKRAEDFLKASGIADTVLFGPEPEFFMFDNITFSNTMGHSFVKIESEEAAWN
SGTEYEHGNKGHRPFVKGGYFPVAPVDSSQDIRAAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTLKADEVQVLK
YVIHNVAHAYNKTVTFMPKPMFGDNGSGMHCHQSLAKNGVNLFAGDLYGGLSEMALHYIGGIIKHAKAINAFANPTTNSY
KRLVPGYEAPVMLAYSARNRSASIRIPVVPSPKARRIEVRFPDPAANPYLAFAAQLMAGLDGIINKIHPGDAMDKNLYDL
PPEEAAEVPTVAGSLDEALAALDSDREFLTRGGVFSDDFINSYLELKQHDVDRVRMTPHPLEFELYYSV

Sequences:

>Translated_469_residues
MSAQNVLALIQEHEVKFVDLRFTDTKGKEQHVSIPSHQVNEDFFEDGKMFDGSSIGGWKGINESDMLLMPDAASAKLDPF
TEETTLNIVCDVLEPATMQGYDRDPRSISKRAEDFLKASGIADTVLFGPEPEFFMFDNITFSNTMGHSFVKIESEEAAWN
SGTEYEHGNKGHRPFVKGGYFPVAPVDSSQDIRAAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTLKADEVQVLK
YVIHNVAHAYNKTVTFMPKPMFGDNGSGMHCHQSLAKNGVNLFAGDLYGGLSEMALHYIGGIIKHAKAINAFANPTTNSY
KRLVPGYEAPVMLAYSARNRSASIRIPVVPSPKARRIEVRFPDPAANPYLAFAAQLMAGLDGIINKIHPGDAMDKNLYDL
PPEEAAEVPTVAGSLDEALAALDSDREFLTRGGVFSDDFINSYLELKQHDVDRVRMTPHPLEFELYYSV
>Mature_468_residues
SAQNVLALIQEHEVKFVDLRFTDTKGKEQHVSIPSHQVNEDFFEDGKMFDGSSIGGWKGINESDMLLMPDAASAKLDPFT
EETTLNIVCDVLEPATMQGYDRDPRSISKRAEDFLKASGIADTVLFGPEPEFFMFDNITFSNTMGHSFVKIESEEAAWNS
GTEYEHGNKGHRPFVKGGYFPVAPVDSSQDIRAAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTLKADEVQVLKY
VIHNVAHAYNKTVTFMPKPMFGDNGSGMHCHQSLAKNGVNLFAGDLYGGLSEMALHYIGGIIKHAKAINAFANPTTNSYK
RLVPGYEAPVMLAYSARNRSASIRIPVVPSPKARRIEVRFPDPAANPYLAFAAQLMAGLDGIINKIHPGDAMDKNLYDLP
PEEAAEVPTVAGSLDEALAALDSDREFLTRGGVFSDDFINSYLELKQHDVDRVRMTPHPLEFELYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=78.2515991471215, Blast_Score=790, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=174, Percent_Identity=32.183908045977, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51810; Mature: 51679

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQNVLALIQEHEVKFVDLRFTDTKGKEQHVSIPSHQVNEDFFEDGKMFDGSSIGGWKG
CCHHHHHHHHHHCCEEEEEEEEECCCCCCCEECCCCCCCCHHHHHCCCEECCCCCCCCCC
INESDMLLMPDAASAKLDPFTEETTLNIVCDVLEPATMQGYDRDPRSISKRAEDFLKASG
CCCCCEEEECCCCCCCCCCCCCCCHHHHHEEHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLFGPEPEFFMFDNITFSNTMGHSFVKIESEEAAWNSGTEYEHGNKGHRPFVKGGY
CCCEEEECCCCCEEEECCEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDIRAAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTLKADEVQVLK
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEEEHHHHHHHH
YVIHNVAHAYNKTVTFMPKPMFGDNGSGMHCHQSLAKNGVNLFAGDLYGGLSEMALHYIG
HHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEEHHHHCCHHHHHHHHHH
GIIKHAKAINAFANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVPSPKARRIEVR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEEE
FPDPAANPYLAFAAQLMAGLDGIINKIHPGDAMDKNLYDLPPEEAAEVPTVAGSLDEALA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHCHHHHHHH
ALDSDREFLTRGGVFSDDFINSYLELKQHDVDRVRMTPHPLEFELYYSV
HHCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEEC
>Mature Secondary Structure 
SAQNVLALIQEHEVKFVDLRFTDTKGKEQHVSIPSHQVNEDFFEDGKMFDGSSIGGWKG
CHHHHHHHHHHCCEEEEEEEEECCCCCCCEECCCCCCCCHHHHHCCCEECCCCCCCCCC
INESDMLLMPDAASAKLDPFTEETTLNIVCDVLEPATMQGYDRDPRSISKRAEDFLKASG
CCCCCEEEECCCCCCCCCCCCCCCHHHHHEEHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
IADTVLFGPEPEFFMFDNITFSNTMGHSFVKIESEEAAWNSGTEYEHGNKGHRPFVKGGY
CCCEEEECCCCCEEEECCEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
FPVAPVDSSQDIRAAMCLVLEEMGQVVEAHHHEVATAGQNEIATRFNTLTLKADEVQVLK
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEEEHHHHHHHH
YVIHNVAHAYNKTVTFMPKPMFGDNGSGMHCHQSLAKNGVNLFAGDLYGGLSEMALHYIG
HHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEEHHHHCCHHHHHHHHHH
GIIKHAKAINAFANPTTNSYKRLVPGYEAPVMLAYSARNRSASIRIPVVPSPKARRIEVR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEEE
FPDPAANPYLAFAAQLMAGLDGIINKIHPGDAMDKNLYDLPPEEAAEVPTVAGSLDEALA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHCHHHHHHH
ALDSDREFLTRGGVFSDDFINSYLELKQHDVDRVRMTPHPLEFELYYSV
HHCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]