Click here to switch to the map view.

The map label for this gene is dhaR [H]

Identifier: 145300901

GI number: 145300901

Start: 4347709

End: 4349610

Strand: Direct

Name: dhaR [H]

Synonym: ASA_4047

Alternate gene names: 145300901

Gene position: 4347709-4349610 (Clockwise)

Preceding gene: 145300900

Following gene: 145300906

Centisome position: 92.46

GC content: 67.61

Gene sequence:

>1902_bases
ATGCCATCTGACCTTCAGCCACTGCAGCCCTGGCCTGACTATCTGCAGGCCTCCTGGCTGCGCTGCGCCCACCAGACCAG
CGCCACCCTCTGGCATCCGCCCCACAGCGCCAAGGGGCAAACCTTGCAGAGCCTGCGTCAGCGCAAGCGGGACTTGCTCA
CCACCGGCGAGATGGCCCTCGAAGATCTCTACGAATTTATGGAGCGGCGCCCCTGCGCCCTGCTGCTTACCGACGAGAGC
GGTTGTGTGCTGGCCCGCACCGGCCACCCCGGCACGCTGGCGGAGCTGGCAGCGCTCGGGTTTGGCCCCGGCGCCTTTTT
CAGCGAGGGGCGCATCGGCACCAATGCCATCAATCTGGCGGCGCTGGAAGGAGTGCCGCTCTGCGTCAGCGGCCCCCAGC
ACTTCAACCAGACCCTGCACCCCTGGCACAGTTGCGCCAGTCCGGTCTACAACAGCAATGGCCGTCAGGTCGCCATCATC
GCCCTGATCTGCAAGGTGGAGGAGGCGAGCGCTGGCGATCTGGCGCTCACCGTCAGCGCCGGGCGCGAGCTGGCCCATCT
GCTGCAGATGGAGACTCTGATGCAGGAGTCCCAGCACCACCTGAGCGAGCTCTACGCCCTGCTCGATGGCATGGATGACG
GCGTGCTGGCCTGGGATCCCCAGGGCCGACTGCGCTACCTCAACGGGCTGGCGGCCAGTCGGCTGCGGCTCGATCCCGCG
CTCTGCCTCGGCCAGCCGCTGGAGCAGCACCTGCTGTTGCCACAGAGGTTGCAGCTCGCCATCAAGGGGCAGACACCGCT
CAGTCATGTGGAGGTAACGCTGGAGCGGCTCGGCGCTCAGGAGGGTGAGTTCATCAATGCGCTGATAAGCCTCAAGCCGC
TGCCGGGGCCGGAGGGGGTCAGCTTTATCGCCCTGCTGCATCCGGTTGACCGGCTGCGGGCCATTCACCATCTGCGCCAG
ACGGTGCCCGAACTCTCCGCCCTGGTGGGCGAATCGAGTGCCATGCGCAAGCTGCTGCGCTATGCCCGGCAGGCGGCCCA
GAGCACTGGCCCCATCCTGCTGCGCGGCGAGGATGAGGTGGGCAAGGCCCAGCTGGCGGAGGCGATCCACTTTGGCAGCG
AACAGGCCGCTGGGCCCCTCATTACCCTCAACTGTCAGGCGCTGCCGAGCGAGCGGATGGCGTTGGAGCTGATGGGCTCT
GACGAAGCGGGGCAGGAGCGGGCAGGCAAGTTCGAGCTGGCCCACGGCGGCACGTTAGTGCTGGAGCAGGTGGAGTGCCT
CTCTGCCCCCATGCAGGCAGCGCTGCTGCAACTGCTCAAGACCGGCCGTATCCAGCGTTTCGATTCGGCCCGCATCCTGC
CGCTCAAGGTGCGGATCATCGCCACCAGCAGCGCCCCGCTGGAGCAACTGGTGCAGGAGGGGCTGTTTGGCCGCCAGCTG
CTCTATGCGCTGCAGGCGTGCGAGCTGTGGCTGCCGGGGCTGCGGGAACGCGCGGCGGATCTACCGGGTCTGATCAGCCA
GCAACTAGGGGCGCAGGGGCGCGAGCCGGTGCGCCAGTTCAGTCCGGCGGCCCTCGATTGTCTGCTCGCCTATCCTTGGC
CCGGCAATCTGGGGGAGCTGCGCAGCGCGGTCGAGCATGCCCTGCTCCATGCCGGTCAGGGGCGGATCCCGCCGGAGGCG
CTGCCCGCCGCCATTCGTCACGGCTATCAGCTGGTGGCGAATGAGGTGGTGGGGCAACCGCTGCTCACCCTGGCCGAGCT
GGAGCGGCAGGCCATTTTGCGCTGCGCCCACGCCTGCAAGGGACAGGTGAGCCAGATGGCCCAGCATCTTGGCATCAGTC
GCACCACCCTCTGGCGGCGGCTGAAACTGCTGGGTATCGATCCCGCCGACTATCACGGCTGA

Upstream 100 bases:

>100_bases
ACCTGAATGGCATTACCCAGCCTCTGAGGCAGGTCACTGAATCGGGCTCGCTGGCGTGGTTAAATGGTCTGCTCACATCT
TGCCGGATATTGCTGTGACC

Downstream 100 bases:

>100_bases
CGGCAACCCGAGCCGTCACCAACCGGCCCGCGAAAATGAAAAGGCCCCGCAGTTGCGGGGCCGTTTTATATGGCAAGGTC
AGCAGTGGCGCGGTTACAGC

Product: DNA-binding transcriptional regulator DhaR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MPSDLQPLQPWPDYLQASWLRCAHQTSATLWHPPHSAKGQTLQSLRQRKRDLLTTGEMALEDLYEFMERRPCALLLTDES
GCVLARTGHPGTLAELAALGFGPGAFFSEGRIGTNAINLAALEGVPLCVSGPQHFNQTLHPWHSCASPVYNSNGRQVAII
ALICKVEEASAGDLALTVSAGRELAHLLQMETLMQESQHHLSELYALLDGMDDGVLAWDPQGRLRYLNGLAASRLRLDPA
LCLGQPLEQHLLLPQRLQLAIKGQTPLSHVEVTLERLGAQEGEFINALISLKPLPGPEGVSFIALLHPVDRLRAIHHLRQ
TVPELSALVGESSAMRKLLRYARQAAQSTGPILLRGEDEVGKAQLAEAIHFGSEQAAGPLITLNCQALPSERMALELMGS
DEAGQERAGKFELAHGGTLVLEQVECLSAPMQAALLQLLKTGRIQRFDSARILPLKVRIIATSSAPLEQLVQEGLFGRQL
LYALQACELWLPGLRERAADLPGLISQQLGAQGREPVRQFSPAALDCLLAYPWPGNLGELRSAVEHALLHAGQGRIPPEA
LPAAIRHGYQLVANEVVGQPLLTLAELERQAILRCAHACKGQVSQMAQHLGISRTTLWRRLKLLGIDPADYHG

Sequences:

>Translated_633_residues
MPSDLQPLQPWPDYLQASWLRCAHQTSATLWHPPHSAKGQTLQSLRQRKRDLLTTGEMALEDLYEFMERRPCALLLTDES
GCVLARTGHPGTLAELAALGFGPGAFFSEGRIGTNAINLAALEGVPLCVSGPQHFNQTLHPWHSCASPVYNSNGRQVAII
ALICKVEEASAGDLALTVSAGRELAHLLQMETLMQESQHHLSELYALLDGMDDGVLAWDPQGRLRYLNGLAASRLRLDPA
LCLGQPLEQHLLLPQRLQLAIKGQTPLSHVEVTLERLGAQEGEFINALISLKPLPGPEGVSFIALLHPVDRLRAIHHLRQ
TVPELSALVGESSAMRKLLRYARQAAQSTGPILLRGEDEVGKAQLAEAIHFGSEQAAGPLITLNCQALPSERMALELMGS
DEAGQERAGKFELAHGGTLVLEQVECLSAPMQAALLQLLKTGRIQRFDSARILPLKVRIIATSSAPLEQLVQEGLFGRQL
LYALQACELWLPGLRERAADLPGLISQQLGAQGREPVRQFSPAALDCLLAYPWPGNLGELRSAVEHALLHAGQGRIPPEA
LPAAIRHGYQLVANEVVGQPLLTLAELERQAILRCAHACKGQVSQMAQHLGISRTTLWRRLKLLGIDPADYHG
>Mature_632_residues
PSDLQPLQPWPDYLQASWLRCAHQTSATLWHPPHSAKGQTLQSLRQRKRDLLTTGEMALEDLYEFMERRPCALLLTDESG
CVLARTGHPGTLAELAALGFGPGAFFSEGRIGTNAINLAALEGVPLCVSGPQHFNQTLHPWHSCASPVYNSNGRQVAIIA
LICKVEEASAGDLALTVSAGRELAHLLQMETLMQESQHHLSELYALLDGMDDGVLAWDPQGRLRYLNGLAASRLRLDPAL
CLGQPLEQHLLLPQRLQLAIKGQTPLSHVEVTLERLGAQEGEFINALISLKPLPGPEGVSFIALLHPVDRLRAIHHLRQT
VPELSALVGESSAMRKLLRYARQAAQSTGPILLRGEDEVGKAQLAEAIHFGSEQAAGPLITLNCQALPSERMALELMGSD
EAGQERAGKFELAHGGTLVLEQVECLSAPMQAALLQLLKTGRIQRFDSARILPLKVRIIATSSAPLEQLVQEGLFGRQLL
YALQACELWLPGLRERAADLPGLISQQLGAQGREPVRQFSPAALDCLLAYPWPGNLGELRSAVEHALLHAGQGRIPPEAL
PAAIRHGYQLVANEVVGQPLLTLAELERQAILRCAHACKGQVSQMAQHLGISRTTLWRRLKLLGIDPADYHG

Specific function: Positively regulates the dhaKLM operon from a sigma-70 promoter. Represses its own synthesis [H]

COG id: COG3284

COG function: function code QK; Transcriptional activator of acetoin/glycerol metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081858, Length=627, Percent_Identity=42.1052631578947, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1790437, Length=318, Percent_Identity=34.2767295597484, Blast_Score=165, Evalue=9e-42,
Organism=Escherichia coli, GI1788550, Length=335, Percent_Identity=32.8358208955224, Blast_Score=162, Evalue=8e-41,
Organism=Escherichia coli, GI87082117, Length=322, Percent_Identity=31.6770186335404, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1788905, Length=349, Percent_Identity=30.6590257879656, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1789087, Length=333, Percent_Identity=31.2312312312312, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1786524, Length=322, Percent_Identity=32.9192546583851, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1790299, Length=339, Percent_Identity=30.0884955752212, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789233, Length=456, Percent_Identity=26.9736842105263, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787583, Length=311, Percent_Identity=27.9742765273312, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI87082152, Length=245, Percent_Identity=35.9183673469388, Blast_Score=119, Evalue=9e-28,
Organism=Escherichia coli, GI87081872, Length=241, Percent_Identity=33.195020746888, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789828, Length=250, Percent_Identity=32, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 68916; Mature: 68784

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSDLQPLQPWPDYLQASWLRCAHQTSATLWHPPHSAKGQTLQSLRQRKRDLLTTGEMAL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EDLYEFMERRPCALLLTDESGCVLARTGHPGTLAELAALGFGPGAFFSEGRIGTNAINLA
HHHHHHHHCCCCEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
ALEGVPLCVSGPQHFNQTLHPWHSCASPVYNSNGRQVAIIALICKVEEASAGDLALTVSA
EECCCCEEECCHHHHHHHHCHHHHHCCCCCCCCCCEEEEEEEEEHHCCCCCCCEEEEECC
GRELAHLLQMETLMQESQHHLSELYALLDGMDDGVLAWDPQGRLRYLNGLAASRLRLDPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHH
LCLGQPLEQHLLLPQRLQLAIKGQTPLSHVEVTLERLGAQEGEFINALISLKPLPGPEGV
HHHCCCHHHHCCCCHHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
SFIALLHPVDRLRAIHHLRQTVPELSALVGESSAMRKLLRYARQAAQSTGPILLRGEDEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
GKAQLAEAIHFGSEQAAGPLITLNCQALPSERMALELMGSDEAGQERAGKFELAHGGTLV
CHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHEEEEECCCCCCHHHCCCEEECCCCHHH
LEQVECLSAPMQAALLQLLKTGRIQRFDSARILPLKVRIIATSSAPLEQLVQEGLFGRQL
HHHHHHHHCCHHHHHHHHHHHCCCEECCCCCEEEEEEEEEEECCCHHHHHHHCCCHHHHH
LYALQACELWLPGLRERAADLPGLISQQLGAQGREPVRQFSPAALDCLLAYPWPGNLGEL
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCCCCCHHHHCCCHHHHHEEECCCCCCHHHH
RSAVEHALLHAGQGRIPPEALPAAIRHGYQLVANEVVGQPLLTLAELERQAILRCAHACK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
GQVSQMAQHLGISRTTLWRRLKLLGIDPADYHG
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PSDLQPLQPWPDYLQASWLRCAHQTSATLWHPPHSAKGQTLQSLRQRKRDLLTTGEMAL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EDLYEFMERRPCALLLTDESGCVLARTGHPGTLAELAALGFGPGAFFSEGRIGTNAINLA
HHHHHHHHCCCCEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
ALEGVPLCVSGPQHFNQTLHPWHSCASPVYNSNGRQVAIIALICKVEEASAGDLALTVSA
EECCCCEEECCHHHHHHHHCHHHHHCCCCCCCCCCEEEEEEEEEHHCCCCCCCEEEEECC
GRELAHLLQMETLMQESQHHLSELYALLDGMDDGVLAWDPQGRLRYLNGLAASRLRLDPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHH
LCLGQPLEQHLLLPQRLQLAIKGQTPLSHVEVTLERLGAQEGEFINALISLKPLPGPEGV
HHHCCCHHHHCCCCHHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
SFIALLHPVDRLRAIHHLRQTVPELSALVGESSAMRKLLRYARQAAQSTGPILLRGEDEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
GKAQLAEAIHFGSEQAAGPLITLNCQALPSERMALELMGSDEAGQERAGKFELAHGGTLV
CHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHEEEEECCCCCCHHHCCCEEECCCCHHH
LEQVECLSAPMQAALLQLLKTGRIQRFDSARILPLKVRIIATSSAPLEQLVQEGLFGRQL
HHHHHHHHCCHHHHHHHHHHHCCCEECCCCCEEEEEEEEEEECCCHHHHHHHCCCHHHHH
LYALQACELWLPGLRERAADLPGLISQQLGAQGREPVRQFSPAALDCLLAYPWPGNLGEL
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCCCCCHHHHCCCHHHHHEEECCCCCCHHHH
RSAVEHALLHAGQGRIPPEALPAAIRHGYQLVANEVVGQPLLTLAELERQAILRCAHACK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
GQVSQMAQHLGISRTTLWRRLKLLGIDPADYHG
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]