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The map label for this gene is intB [H]

Identifier: 145300733

GI number: 145300733

Start: 4156238

End: 4157452

Strand: Direct

Name: intB [H]

Synonym: ASA_3872

Alternate gene names: 145300733

Gene position: 4156238-4157452 (Clockwise)

Preceding gene: 145300732

Following gene: 145300736

Centisome position: 88.39

GC content: 58.19

Gene sequence:

>1215_bases
ATGCCTTTAACTGACATAACAGCCCGGCAGGCCAAGTGGAGCGGCAAGCCGAACGGCGACAAGCTAGCCGATGAGAAGGG
ACTGTTCCTGCTGGTTAACAAGTCCGGCAAGTATTGGAAACTCAAGTATCGCCATGGTGGCAAAGAGAAGAAGCTCGCTC
TGGGGGTTTATCCCGAGGTCTCCCTGAGAGAGGCCAGGGCTAAACGGGATGAAGCTCGCCGGGTGATCGCCGAAGGGATA
GACCCCGGCCTCGTGCGCAAGCAAAGCAAGGTAGCCAGTCGGCTGGTCAGTGAAAATAGCCTCGAGGTGTTAGCGCGGGA
ATGGCATCAGAGCCAGTTCGCGTTGTGGAGCCCAGGCCACGCCACCCGAGTGATCGAGTCGCTGGAGGTGGATGTCTTCC
CCGATCTGGGATTAGTGCCTATCACAGAGCTGACTGCCCCCATGATTCTGGAGGCCCTCCGGAAAGTTGAGAAGCGGGGG
GCAACCGAAACAGCGGGACGGATATTGCAACGGGTCAGCGCGATCATGCGCTACGCCATTCAGACTGGACGAGCCAGCTA
CAACCCGGCTCAAGACCTGAGGGGGGCGCTCAAGGCCACCAAGCAGGAGCACAGACCCGCTTTGCCCCGTTCTGAGCTGC
CAGAATTCTTCCGACGTTTGGAAGCCGAGCCACTTAACCCGGCGACTCGTCTTGCCCTCCATCTGCTGATGTTGACCATG
ACCCGCCCTGGTGAAGTGCGCTTTGCACGTTGGGATGAGTTCGATATGGAACGCGCAGAATGGCGGATCCCTGCTGCACG
GATGAAGATGCGAACCGAGCACCTTGTCCCCCTTTCTCGCCAGGCGTTGGCCGTGCTCGATGAACTGCACCAGCTGACAG
GCCATTGTGAGTTGCTGTTCCCGAGCGAGCGTAAACTGACCAATCCCATGAGTGAGAACACCTTGAGCTATGCAATGGGG
CGGATGGGTTATAAAGGGATCGCTACACCGCACGGATTCAGGGCATTAGCCTCTACCACCTTGAACGAGGAAGGCTTTGA
TCCGGACGTGATAGAGCGCCAGCTTGCCCACGCCGAACGCAACAAGGTGCGGGCAGCCTATCACCGGGCTGAGTATCTGG
ACGACCGGCGCAAGCTGTTGCAGTGGCTGGCCGATTTCTATGACTCGCAACGGGGTAGCAATGTGCTACCAGTGAATTTC
AACCGAGTGGGTTAA

Upstream 100 bases:

>100_bases
CAATCGTGGCCAACCGTTGTCCTGTAAGGCTTTCAGCCAATGCTGATAGGCCCCCACTGCGGCAACTTAGGCCCCCATAA
TTGGATATGGAGGCCCCCAG

Downstream 100 bases:

>100_bases
CGACTTTGCTGAGCCTATCTCGACAGAGAGAAAAGTTGGGAACCTTGCCCACTTGGCTCGGCAATTTATTCAAGGCAAGT
GTATAGAGGCAACACGATGG

Product: phage integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MPLTDITARQAKWSGKPNGDKLADEKGLFLLVNKSGKYWKLKYRHGGKEKKLALGVYPEVSLREARAKRDEARRVIAEGI
DPGLVRKQSKVASRLVSENSLEVLAREWHQSQFALWSPGHATRVIESLEVDVFPDLGLVPITELTAPMILEALRKVEKRG
ATETAGRILQRVSAIMRYAIQTGRASYNPAQDLRGALKATKQEHRPALPRSELPEFFRRLEAEPLNPATRLALHLLMLTM
TRPGEVRFARWDEFDMERAEWRIPAARMKMRTEHLVPLSRQALAVLDELHQLTGHCELLFPSERKLTNPMSENTLSYAMG
RMGYKGIATPHGFRALASTTLNEEGFDPDVIERQLAHAERNKVRAAYHRAEYLDDRRKLLQWLADFYDSQRGSNVLPVNF
NRVG

Sequences:

>Translated_404_residues
MPLTDITARQAKWSGKPNGDKLADEKGLFLLVNKSGKYWKLKYRHGGKEKKLALGVYPEVSLREARAKRDEARRVIAEGI
DPGLVRKQSKVASRLVSENSLEVLAREWHQSQFALWSPGHATRVIESLEVDVFPDLGLVPITELTAPMILEALRKVEKRG
ATETAGRILQRVSAIMRYAIQTGRASYNPAQDLRGALKATKQEHRPALPRSELPEFFRRLEAEPLNPATRLALHLLMLTM
TRPGEVRFARWDEFDMERAEWRIPAARMKMRTEHLVPLSRQALAVLDELHQLTGHCELLFPSERKLTNPMSENTLSYAMG
RMGYKGIATPHGFRALASTTLNEEGFDPDVIERQLAHAERNKVRAAYHRAEYLDDRRKLLQWLADFYDSQRGSNVLPVNF
NRVG
>Mature_403_residues
PLTDITARQAKWSGKPNGDKLADEKGLFLLVNKSGKYWKLKYRHGGKEKKLALGVYPEVSLREARAKRDEARRVIAEGID
PGLVRKQSKVASRLVSENSLEVLAREWHQSQFALWSPGHATRVIESLEVDVFPDLGLVPITELTAPMILEALRKVEKRGA
TETAGRILQRVSAIMRYAIQTGRASYNPAQDLRGALKATKQEHRPALPRSELPEFFRRLEAEPLNPATRLALHLLMLTMT
RPGEVRFARWDEFDMERAEWRIPAARMKMRTEHLVPLSRQALAVLDELHQLTGHCELLFPSERKLTNPMSENTLSYAMGR
MGYKGIATPHGFRALASTTLNEEGFDPDVIERQLAHAERNKVRAAYHRAEYLDDRRKLLQWLADFYDSQRGSNVLPVNFN
RVG

Specific function: Unknown

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=405, Percent_Identity=37.7777777777778, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1788690, Length=372, Percent_Identity=37.3655913978495, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI145693166, Length=401, Percent_Identity=38.4039900249377, Blast_Score=242, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46015; Mature: 45883

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTDITARQAKWSGKPNGDKLADEKGLFLLVNKSGKYWKLKYRHGGKEKKLALGVYPEV
CCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEEEECCCCH
SLREARAKRDEARRVIAEGIDPGLVRKQSKVASRLVSENSLEVLAREWHQSQFALWSPGH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCH
ATRVIESLEVDVFPDLGLVPITELTAPMILEALRKVEKRGATETAGRILQRVSAIMRYAI
HHHHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QTGRASYNPAQDLRGALKATKQEHRPALPRSELPEFFRRLEAEPLNPATRLALHLLMLTM
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TRPGEVRFARWDEFDMERAEWRIPAARMKMRTEHLVPLSRQALAVLDELHQLTGHCELLF
CCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEE
PSERKLTNPMSENTLSYAMGRMGYKGIATPHGFRALASTTLNEEGFDPDVIERQLAHAER
CCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
NKVRAAYHRAEYLDDRRKLLQWLADFYDSQRGSNVLPVNFNRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
>Mature Secondary Structure 
PLTDITARQAKWSGKPNGDKLADEKGLFLLVNKSGKYWKLKYRHGGKEKKLALGVYPEV
CCCCCCHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEEEECCCCH
SLREARAKRDEARRVIAEGIDPGLVRKQSKVASRLVSENSLEVLAREWHQSQFALWSPGH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCH
ATRVIESLEVDVFPDLGLVPITELTAPMILEALRKVEKRGATETAGRILQRVSAIMRYAI
HHHHHHHHCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QTGRASYNPAQDLRGALKATKQEHRPALPRSELPEFFRRLEAEPLNPATRLALHLLMLTM
HHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TRPGEVRFARWDEFDMERAEWRIPAARMKMRTEHLVPLSRQALAVLDELHQLTGHCELLF
CCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEE
PSERKLTNPMSENTLSYAMGRMGYKGIATPHGFRALASTTLNEEGFDPDVIERQLAHAER
CCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
NKVRAAYHRAEYLDDRRKLLQWLADFYDSQRGSNVLPVNFNRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]