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The map label for this gene is metB [H]

Identifier: 145300663

GI number: 145300663

Start: 4087406

End: 4088578

Strand: Direct

Name: metB [H]

Synonym: ASA_3795

Alternate gene names: 145300663

Gene position: 4087406-4088578 (Clockwise)

Preceding gene: 145300659

Following gene: 145300664

Centisome position: 86.92

GC content: 63.0

Gene sequence:

>1173_bases
ATGAGTCATAAGGCCACCCTGGCGGTACGCACCGGGATTGAAACAGACACGCAGCACGGTGCCGTGGTGCCGCCCATTTA
CCTCTCCAGCAACTACACATTTGCCGACTTTGGCGAGCCTCGTCAATACGATTATGCCCGCTCCGGCAACCCGACCCGTA
CCAATCTGGCGGACGCCCTGGCGGCGCTGGAAGGGGGGGCTGGCGCCGTTGTAACCGGCACTGGCATGGGGGCCATCCAT
CTGGTGACCACGGCTCTGCTCAAGGCGGGGGATCTGCTGATCGCCCCTCACGACTGTTACGGCGGTACCTGGCGCCTGTT
CGAGTATCTGGCTGCCAAGGGCCACTATCGGGTGCAGTTTGTCGATCAGGGTGATGAGGCCGCACTGGCAGAGGCGCTGA
CACAGAAGCCGGCGCTGGTGTGGGTGGAGACCCCTTCCAACCCCTTGCTGCGGGTAGTGGACATCACCGCCATCGCCGCC
GCCTCCCATGAGGTGGGTGCCCAGGTGGTGGTGGACAACACCTTCCTCTCTCCGCTGCTGCAACAGCCGTTGTCGCTGGG
TGCCGATGTTGTGGTGCACTCCACCACCAAGTACATCAATGGCCACTCCGATGTGGTGGGGGGCGTGGCCATTGCCAAGG
ATCCCGCGCTGGCTGAATCACTGGTGTGGTGGGCCAACTGTCTGGGACTCACCTCCGGGGCGTTCGACTCCTACCTCACC
CTGCGCGGCCTGCGCACCCTGGCTCCCAGATTGCGCGCCCATCAGGAGAACACGGACCGCATTCTCGCCTTCCTGCAGCA
ACAGCCGCTGGTCAAAAAAATCTATCATCCCAGCCTGCAGAGCCATCCAGGCCATGATATTGCGCGCCGTCAACAGTCCG
GCTTCGGCGCCATGCTAAGTTTTGAACTCGATTGCAGCGAGGCGGGATTACGCACCTTCCTCGCGGCCCTTACCCTCTTC
TCGGTAGCCGAGTCTCTCGGCGGGGTGGAGAGTCTGGTGGCCCATCCGGCCAGCATGACCCACAGGGCCATGACACCTGC
GGCGCAGCAGGCGGCGGGCATCAGCGCCCAGCTGCTGCGTCTGTCGGTGGGTATTGAACACGGTGAAGATCTGGTGGCCG
ATCTGGCTCAGGCTTTTGAACAGGCGCAGCTGGCAGAACAGGTGGCGAAGTAA

Upstream 100 bases:

>100_bases
TCAAGGAGAGCGGAAATGACCCACAAGGCTACCCTGCCGGTACGTTGCATCGGAAATCGAGCCGGATCAATCGCCCAGTC
ACAAAGCCAAGGAGAGCAGC

Downstream 100 bases:

>100_bases
TGGAACAGCAGCAAGGAGATATAGCGGTGGCAACACAAGCAGGGTTGAAGCGGCGGCAGGTACACAAGTTCGGTGGCAGC
AGTCTGGCGGATCCGGTCTG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSHKATLAVRTGIETDTQHGAVVPPIYLSSNYTFADFGEPRQYDYARSGNPTRTNLADALAALEGGAGAVVTGTGMGAIH
LVTTALLKAGDLLIAPHDCYGGTWRLFEYLAAKGHYRVQFVDQGDEAALAEALTQKPALVWVETPSNPLLRVVDITAIAA
ASHEVGAQVVVDNTFLSPLLQQPLSLGADVVVHSTTKYINGHSDVVGGVAIAKDPALAESLVWWANCLGLTSGAFDSYLT
LRGLRTLAPRLRAHQENTDRILAFLQQQPLVKKIYHPSLQSHPGHDIARRQQSGFGAMLSFELDCSEAGLRTFLAALTLF
SVAESLGGVESLVAHPASMTHRAMTPAAQQAAGISAQLLRLSVGIEHGEDLVADLAQAFEQAQLAEQVAK

Sequences:

>Translated_390_residues
MSHKATLAVRTGIETDTQHGAVVPPIYLSSNYTFADFGEPRQYDYARSGNPTRTNLADALAALEGGAGAVVTGTGMGAIH
LVTTALLKAGDLLIAPHDCYGGTWRLFEYLAAKGHYRVQFVDQGDEAALAEALTQKPALVWVETPSNPLLRVVDITAIAA
ASHEVGAQVVVDNTFLSPLLQQPLSLGADVVVHSTTKYINGHSDVVGGVAIAKDPALAESLVWWANCLGLTSGAFDSYLT
LRGLRTLAPRLRAHQENTDRILAFLQQQPLVKKIYHPSLQSHPGHDIARRQQSGFGAMLSFELDCSEAGLRTFLAALTLF
SVAESLGGVESLVAHPASMTHRAMTPAAQQAAGISAQLLRLSVGIEHGEDLVADLAQAFEQAQLAEQVAK
>Mature_389_residues
SHKATLAVRTGIETDTQHGAVVPPIYLSSNYTFADFGEPRQYDYARSGNPTRTNLADALAALEGGAGAVVTGTGMGAIHL
VTTALLKAGDLLIAPHDCYGGTWRLFEYLAAKGHYRVQFVDQGDEAALAEALTQKPALVWVETPSNPLLRVVDITAIAAA
SHEVGAQVVVDNTFLSPLLQQPLSLGADVVVHSTTKYINGHSDVVGGVAIAKDPALAESLVWWANCLGLTSGAFDSYLTL
RGLRTLAPRLRAHQENTDRILAFLQQQPLVKKIYHPSLQSHPGHDIARRQQSGFGAMLSFELDCSEAGLRTFLAALTLFS
VAESLGGVESLVAHPASMTHRAMTPAAQQAAGISAQLLRLSVGIEHGEDLVADLAQAFEQAQLAEQVAK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=364, Percent_Identity=42.032967032967, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI299473759, Length=366, Percent_Identity=37.9781420765027, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI25453487, Length=363, Percent_Identity=36.0881542699724, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI1790375, Length=380, Percent_Identity=65.7894736842105, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1789383, Length=339, Percent_Identity=32.1533923303835, Blast_Score=159, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17538055, Length=384, Percent_Identity=43.4895833333333, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17560056, Length=384, Percent_Identity=43.2291666666667, Blast_Score=286, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=33.5135135135135, Blast_Score=204, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6319307, Length=388, Percent_Identity=39.1752577319588, Blast_Score=252, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6321254, Length=405, Percent_Identity=35.3086419753086, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=30.8755760368664, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=325, Percent_Identity=30.1538461538462, Blast_Score=149, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6321904, Length=391, Percent_Identity=30.1790281329923, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323557, Length=271, Percent_Identity=26.5682656826568, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322970, Length=270, Percent_Identity=25.5555555555556, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922554, Length=384, Percent_Identity=41.40625, Blast_Score=289, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41471; Mature: 41340

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKATLAVRTGIETDTQHGAVVPPIYLSSNYTFADFGEPRQYDYARSGNPTRTNLADAL
CCCCEEEEEECCCCCCCCCCCCCCCEEECCCCEECCCCCCCCCCCCCCCCCCHHHHHHHH
AALEGGAGAVVTGTGMGAIHLVTTALLKAGDLLIAPHDCYGGTWRLFEYLAAKGHYRVQF
HHHCCCCCEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEE
VDQGDEAALAEALTQKPALVWVETPSNPLLRVVDITAIAAASHEVGAQVVVDNTFLSPLL
ECCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHHHCCCCCCCEEEECHHHHHHHH
QQPLSLGADVVVHSTTKYINGHSDVVGGVAIAKDPALAESLVWWANCLGLTSGAFDSYLT
HHHHHCCCCEEEECCHHHCCCCCHHHCCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHH
LRGLRTLAPRLRAHQENTDRILAFLQQQPLVKKIYHPSLQSHPGHDIARRQQSGFGAMLS
HHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEE
FELDCSEAGLRTFLAALTLFSVAESLGGVESLVAHPASMTHRAMTPAAQQAAGISAQLLR
EEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCHHHHHHCCCHHHHHH
LSVGIEHGEDLVADLAQAFEQAQLAEQVAK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHKATLAVRTGIETDTQHGAVVPPIYLSSNYTFADFGEPRQYDYARSGNPTRTNLADAL
CCCEEEEEECCCCCCCCCCCCCCCEEECCCCEECCCCCCCCCCCCCCCCCCHHHHHHHH
AALEGGAGAVVTGTGMGAIHLVTTALLKAGDLLIAPHDCYGGTWRLFEYLAAKGHYRVQF
HHHCCCCCEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEE
VDQGDEAALAEALTQKPALVWVETPSNPLLRVVDITAIAAASHEVGAQVVVDNTFLSPLL
ECCCCHHHHHHHHHCCCCEEEEECCCCCCEEEEEHHHHHHCCCCCCCEEEECHHHHHHHH
QQPLSLGADVVVHSTTKYINGHSDVVGGVAIAKDPALAESLVWWANCLGLTSGAFDSYLT
HHHHHCCCCEEEECCHHHCCCCCHHHCCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHH
LRGLRTLAPRLRAHQENTDRILAFLQQQPLVKKIYHPSLQSHPGHDIARRQQSGFGAMLS
HHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEE
FELDCSEAGLRTFLAALTLFSVAESLGGVESLVAHPASMTHRAMTPAAQQAAGISAQLLR
EEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCHHHHHHCCCHHHHHH
LSVGIEHGEDLVADLAQAFEQAQLAEQVAK
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]