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The map label for this gene is ksgA [H]

Identifier: 145300235

GI number: 145300235

Start: 3624603

End: 3625430

Strand: Direct

Name: ksgA [H]

Synonym: ASA_3350

Alternate gene names: 145300235

Gene position: 3624603-3625430 (Clockwise)

Preceding gene: 145300234

Following gene: 145300236

Centisome position: 77.08

GC content: 60.99

Gene sequence:

>828_bases
ATGAGCAGTAAGGTGCAGAGCAACAAGGTGCACATGGGTCACACGGCCCGTAAGCGTTTCGGTCAGAACTTCTTGCATGA
CCGCTATGTGATCGATCAGATAGTGGCCGCCATCAATCCGCAGCCGGGTCAGAATCTGGTGGAGATAGGCCCGGGCCTCG
CCGCCCTGACCGAACCGGTCGCCTCCCAGATGGACAAGATGACAGTGGTCGAGCTGGACCGGGACTTGGCCGCGCGCCTG
CGCGAGCACCCGACCCTCAAGGACAAGCTGACCGTGATCGAGGCCGATGCCATGCGCTTCGACTTCGGCACTCTGATGGG
GGAAGGCAAGGGGCCGCTGCGCATCTTCGGCAACCTGCCCTACAACATCTCCACGCCGCTCATCTTCCACCTGTGCGATT
TCGCCGACCGGGTAGAAGACATGCACTTCATGCTGCAAAAAGAAGTGGTATTGCGGATGTCTGCCGGCCCGGGCAGCAAG
GCCTATGGCCGCCTGAGCGTCATGGTGCAGTACTACTGCCAGGTGATCCCCGTGCTGGAAGTGGGCCCTGGCGCCTTCAA
ACCGGCACCGAAAGTGGATTCAGCCGTGGTCCGTCTGGTGCCGCACAAGAACCCGACCATAGTCGCCAAAGACATCCGCT
GCCTGAGCCGCGTCTGTACCGAAGGCTTTGGCCAGCGCCGCAAGACCATCCGCAACAGCTTCGCCAACTTCATCACGGAC
GCTCAGCTGACCGAGCTTGGTATCGATGGCAACCTGCGCCCGGAGAATCTCTCCCTGGAGCAGTTCGTCATGATCGCCAA
CTGGCTGGCGGATCAACAACAGGCCTGA

Upstream 100 bases:

>100_bases
CACGGCACTGCGCTGGATCTGGCAGGCAAGAGCCTGGCGGACCCGGGCAGCCTGATCACCGCGATTAACCACGCCATCAA
GATGGTAGAGAAAAAAAGAT

Downstream 100 bases:

>100_bases
TCCCGTGGCATACCCGCATATCGTTATCCGCCCATACCCGCGTTATGTGGCGGATACCCAGGACCCTTACCAGTTCCACT
ATCTGATCGAGATCGAGAAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSSKVQSNKVHMGHTARKRFGQNFLHDRYVIDQIVAAINPQPGQNLVEIGPGLAALTEPVASQMDKMTVVELDRDLAARL
REHPTLKDKLTVIEADAMRFDFGTLMGEGKGPLRIFGNLPYNISTPLIFHLCDFADRVEDMHFMLQKEVVLRMSAGPGSK
AYGRLSVMVQYYCQVIPVLEVGPGAFKPAPKVDSAVVRLVPHKNPTIVAKDIRCLSRVCTEGFGQRRKTIRNSFANFITD
AQLTELGIDGNLRPENLSLEQFVMIANWLADQQQA

Sequences:

>Translated_275_residues
MSSKVQSNKVHMGHTARKRFGQNFLHDRYVIDQIVAAINPQPGQNLVEIGPGLAALTEPVASQMDKMTVVELDRDLAARL
REHPTLKDKLTVIEADAMRFDFGTLMGEGKGPLRIFGNLPYNISTPLIFHLCDFADRVEDMHFMLQKEVVLRMSAGPGSK
AYGRLSVMVQYYCQVIPVLEVGPGAFKPAPKVDSAVVRLVPHKNPTIVAKDIRCLSRVCTEGFGQRRKTIRNSFANFITD
AQLTELGIDGNLRPENLSLEQFVMIANWLADQQQA
>Mature_274_residues
SSKVQSNKVHMGHTARKRFGQNFLHDRYVIDQIVAAINPQPGQNLVEIGPGLAALTEPVASQMDKMTVVELDRDLAARLR
EHPTLKDKLTVIEADAMRFDFGTLMGEGKGPLRIFGNLPYNISTPLIFHLCDFADRVEDMHFMLQKEVVLRMSAGPGSKA
YGRLSVMVQYYCQVIPVLEVGPGAFKPAPKVDSAVVRLVPHKNPTIVAKDIRCLSRVCTEGFGQRRKTIRNSFANFITDA
QLTELGIDGNLRPENLSLEQFVMIANWLADQQQA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=276, Percent_Identity=31.8840579710145, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI7657198, Length=191, Percent_Identity=33.5078534031414, Blast_Score=98, Evalue=9e-21,
Organism=Escherichia coli, GI1786236, Length=265, Percent_Identity=65.2830188679245, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25141369, Length=298, Percent_Identity=27.1812080536913, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25146882, Length=218, Percent_Identity=31.1926605504587, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324989, Length=247, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21358017, Length=239, Percent_Identity=30.5439330543933, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21357273, Length=279, Percent_Identity=27.9569892473118, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30645; Mature: 30514

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKVQSNKVHMGHTARKRFGQNFLHDRYVIDQIVAAINPQPGQNLVEIGPGLAALTEPV
CCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHH
ASQMDKMTVVELDRDLAARLREHPTLKDKLTVIEADAMRFDFGTLMGEGKGPLRIFGNLP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEHHHHHHHHHHHHCCCCCCEEEECCCC
YNISTPLIFHLCDFADRVEDMHFMLQKEVVLRMSAGPGSKAYGRLSVMVQYYCQVIPVLE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHEE
VGPGAFKPAPKVDSAVVRLVPHKNPTIVAKDIRCLSRVCTEGFGQRRKTIRNSFANFITD
CCCCCCCCCCCCCCHHEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AQLTELGIDGNLRPENLSLEQFVMIANWLADQQQA
CHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSKVQSNKVHMGHTARKRFGQNFLHDRYVIDQIVAAINPQPGQNLVEIGPGLAALTEPV
CCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHH
ASQMDKMTVVELDRDLAARLREHPTLKDKLTVIEADAMRFDFGTLMGEGKGPLRIFGNLP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEHHHHHHHHHHHHCCCCCCEEEECCCC
YNISTPLIFHLCDFADRVEDMHFMLQKEVVLRMSAGPGSKAYGRLSVMVQYYCQVIPVLE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHEE
VGPGAFKPAPKVDSAVVRLVPHKNPTIVAKDIRCLSRVCTEGFGQRRKTIRNSFANFITD
CCCCCCCCCCCCCCHHEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AQLTELGIDGNLRPENLSLEQFVMIANWLADQQQA
CHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA