BacMap

Definition	Aeromonas salmonicida subsp. salmonicida A449, complete genome.
Accession	NC_009348
Length	4,702,402

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The map label for this gene is dkgB [H]

Identifier: 145299643

GI number: 145299643

Start: 2914617

End: 2915420

Strand: Direct

Name: dkgB [H]

Synonym: ASA_2717

Alternate gene names: 145299643

Gene position: 2914617-2915420 (Clockwise)

Preceding gene: 145299642

Following gene: 145299644

Centisome position: 61.98

GC content: 59.83

Gene sequence:

>804_bases
ATGATGAAAATGCCTGCCATGGGTCTGGGAACCTTTCGCCTGAAAGGTGAGCCACTGCTTACGACCCTGAACACCGGTCT
GACGCTCGGTTATCGCCATATCGATACCGCCCAGATTTACGACAACGAAAGCGAAGTGGGCGAGGTGCTGGCGACGACCT
CGGTGCCCCGCTCGGATATCTACCTCACCACCAAGGTGTGGACCAGCGAGTTTGCCGAAGGCAAGCTGATCCCTAGCCTG
AAAACCAGCCTTGAGAAACTGCGCACCGACTATCTGGATCTGGCGCTGATCCACTGGCCCTCCCCCAACGACGAGGTGCC
GATGGCGGTCTATCTGGAGCAGCTGGTCGAAGCCAAATCCCTGGGCCTGACCCGGGAAATTGGGGTCTCCAACTTCACGG
TTGCCCAGCTTAAAGAAGCCATCGACATTCTGGGTCCCGGCGCCATCGCTCACCAGCAGGTAGAGATCCACCCCTTGCTG
CAAAACCGCAAGGTGGTGGAATTCTGCCAGGAGCAGGGCATCGCCATCACCGCCTATATGCCGCTGGCTTACGGCAAGGT
GCTGAGTGAGCCGCTCTTGATGGAGATAGGCAAGTACCACAAGGTGTCGGCAGCCCAGGTGTCGCTGGCCTGGCTACTGG
CCCAGGGCATGACGGTGATCCCGAGCTCCACCAAGCGGGAGCATCAAGCCGCCAATCTGGCTGCCCTCGAGGTATCCCTG
AGCAGTGAAGAGATGGCCCAGATCGCCACCTTGGAGCGGGGTGAACGCTGCGCCGACCCGGACTTTGCACCGGCTTGGGA
TTAA

Upstream 100 bases:

>100_bases
TTGACGATGGCGCTGCCAACTTGCACCGCCGCCACGGCCTTTGCACCAAGCCAACGCCTTTGCTCTGGGCAAGGGCCAAA
CACCCCATTTGAGGAAATTG

Downstream 100 bases:

>100_bases
ACTCGGCCCTCACGATCTTTCCCTGCTCAAGGGAGAGTAAAGGCCCCCTTGTGAGGCGCGAGGGTCATAGGGACTCACCC
ATGACGACAGCGCCAGAAAG

Product: 2,5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MMKMPAMGLGTFRLKGEPLLTTLNTGLTLGYRHIDTAQIYDNESEVGEVLATTSVPRSDIYLTTKVWTSEFAEGKLIPSL
KTSLEKLRTDYLDLALIHWPSPNDEVPMAVYLEQLVEAKSLGLTREIGVSNFTVAQLKEAIDILGPGAIAHQQVEIHPLL
QNRKVVEFCQEQGIAITAYMPLAYGKVLSEPLLMEIGKYHKVSAAQVSLAWLLAQGMTVIPSSTKREHQAANLAALEVSL
SSEEMAQIATLERGERCADPDFAPAWD

Sequences:

>Translated_267_residues
MMKMPAMGLGTFRLKGEPLLTTLNTGLTLGYRHIDTAQIYDNESEVGEVLATTSVPRSDIYLTTKVWTSEFAEGKLIPSL
KTSLEKLRTDYLDLALIHWPSPNDEVPMAVYLEQLVEAKSLGLTREIGVSNFTVAQLKEAIDILGPGAIAHQQVEIHPLL
QNRKVVEFCQEQGIAITAYMPLAYGKVLSEPLLMEIGKYHKVSAAQVSLAWLLAQGMTVIPSSTKREHQAANLAALEVSL
SSEEMAQIATLERGERCADPDFAPAWD
>Mature_267_residues
MMKMPAMGLGTFRLKGEPLLTTLNTGLTLGYRHIDTAQIYDNESEVGEVLATTSVPRSDIYLTTKVWTSEFAEGKLIPSL
KTSLEKLRTDYLDLALIHWPSPNDEVPMAVYLEQLVEAKSLGLTREIGVSNFTVAQLKEAIDILGPGAIAHQQVEIHPLL
QNRKVVEFCQEQGIAITAYMPLAYGKVLSEPLLMEIGKYHKVSAAQVSLAWLLAQGMTVIPSSTKREHQAANLAALEVSL
SSEEMAQIATLERGERCADPDFAPAWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=294, Percent_Identity=31.6326530612245, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI5453543, Length=291, Percent_Identity=31.9587628865979, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=31.3131313131313, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI310109922, Length=274, Percent_Identity=32.1167883211679, Blast_Score=144, Evalue=6e-35,
Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=33.1058020477816, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI310109920, Length=291, Percent_Identity=31.6151202749141, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI24497577, Length=291, Percent_Identity=31.2714776632302, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI5174391, Length=291, Percent_Identity=31.2714776632302, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI45446745, Length=291, Percent_Identity=31.2714776632302, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI4503285, Length=291, Percent_Identity=31.2714776632302, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI24497583, Length=290, Percent_Identity=32.0689655172414, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI291291012, Length=266, Percent_Identity=32.3308270676692, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI93277124, Length=305, Percent_Identity=28.1967213114754, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI300116273, Length=270, Percent_Identity=32.962962962963, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI4502049, Length=294, Percent_Identity=32.312925170068, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI300116271, Length=272, Percent_Identity=31.9852941176471, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=31.1688311688312, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=37.5, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=36.5384615384615, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=52.059925093633, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI87082198, Length=261, Percent_Identity=33.7164750957854, Blast_Score=161, Evalue=3e-41,
Organism=Escherichia coli, GI1788081, Length=266, Percent_Identity=34.9624060150376, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787674, Length=253, Percent_Identity=25.6916996047431, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87081735, Length=292, Percent_Identity=27.3972602739726, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788070, Length=317, Percent_Identity=24.9211356466877, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17537075, Length=291, Percent_Identity=33.3333333333333, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17537077, Length=290, Percent_Identity=32.7586206896552, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17561298, Length=272, Percent_Identity=31.9852941176471, Blast_Score=144, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17552492, Length=274, Percent_Identity=33.5766423357664, Blast_Score=143, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI71998625, Length=265, Percent_Identity=32.8301886792453, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17561300, Length=261, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17550248, Length=291, Percent_Identity=30.2405498281787, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17566692, Length=282, Percent_Identity=30.8510638297872, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538386, Length=271, Percent_Identity=32.1033210332103, Blast_Score=123, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17564128, Length=301, Percent_Identity=30.8970099667774, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17537079, Length=297, Percent_Identity=27.9461279461279, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17562292, Length=283, Percent_Identity=27.9151943462898, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324694, Length=294, Percent_Identity=34.0136054421769, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320576, Length=278, Percent_Identity=32.0143884892086, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322556, Length=255, Percent_Identity=28.6274509803922, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321896, Length=308, Percent_Identity=29.8701298701299, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320079, Length=278, Percent_Identity=32.3741007194245, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319625, Length=260, Percent_Identity=31.9230769230769, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644950, Length=305, Percent_Identity=33.7704918032787, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI221378297, Length=325, Percent_Identity=32, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=33.1034482758621, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI21356425, Length=289, Percent_Identity=32.8719723183391, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI45553081, Length=290, Percent_Identity=33.1034482758621, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24662789, Length=293, Percent_Identity=29.3515358361775, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24663317, Length=287, Percent_Identity=29.9651567944251, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI20129731, Length=279, Percent_Identity=30.4659498207885, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI281366140, Length=264, Percent_Identity=32.9545454545455, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=31.1418685121107, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24662781, Length=289, Percent_Identity=30.7958477508651, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 29365; Mature: 29365

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKMPAMGLGTFRLKGEPLLTTLNTGLTLGYRHIDTAQIYDNESEVGEVLATTSVPRSDI
CCCCCCCCCCEEEECCCCEEEEECCCEEECEEECCHHEEECCHHHHHHHHHHCCCCCCCE
YLTTKVWTSEFAEGKLIPSLKTSLEKLRTDYLDLALIHWPSPNDEVPMAVYLEQLVEAKS
EEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHH
LGLTREIGVSNFTVAQLKEAIDILGPGAIAHQQVEIHPLLQNRKVVEFCQEQGIAITAYM
CCCCHHCCCCCCHHHHHHHHHHHCCCCCHHCCEEEEEHHHHCCHHHHHHHHCCCEEEEEH
PLAYGKVLSEPLLMEIGKYHKVSAAQVSLAWLLAQGMTVIPSSTKREHQAANLAALEVSL
HHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCCCCHHHHHCCEEEEEEEC
SSEEMAQIATLERGERCADPDFAPAWD
CHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMKMPAMGLGTFRLKGEPLLTTLNTGLTLGYRHIDTAQIYDNESEVGEVLATTSVPRSDI
CCCCCCCCCCEEEECCCCEEEEECCCEEECEEECCHHEEECCHHHHHHHHHHCCCCCCCE
YLTTKVWTSEFAEGKLIPSLKTSLEKLRTDYLDLALIHWPSPNDEVPMAVYLEQLVEAKS
EEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHH
LGLTREIGVSNFTVAQLKEAIDILGPGAIAHQQVEIHPLLQNRKVVEFCQEQGIAITAYM
CCCCHHCCCCCCHHHHHHHHHHHCCCCCHHCCEEEEEHHHHCCHHHHHHHHCCCEEEEEH
PLAYGKVLSEPLLMEIGKYHKVSAAQVSLAWLLAQGMTVIPSSTKREHQAANLAALEVSL
HHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCCCCHHHHHCCEEEEEEEC
SSEEMAQIATLERGERCADPDFAPAWD
CHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]