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The map label for this gene is mnmC

Identifier: 145299322

GI number: 145299322

Start: 2519445

End: 2521463

Strand: Direct

Name: mnmC

Synonym: ASA_2366

Alternate gene names: 145299322

Gene position: 2519445-2521463 (Clockwise)

Preceding gene: 145299320

Following gene: 145299324

Centisome position: 53.58

GC content: 60.82

Gene sequence:

>2019_bases
ATGACGCGAGCAGAGGGAAACGTGAGTCAAACATCCTTACATCATGCCCGATTGGACTGGAATGAAGCGGGAACTCCAGT
CTCCAGTGACTTCGGGGATGTGTACTTTTCCAATGATAACGGTCTAAGTGAAACCCGCTACGTCTTTTTGCAGCAAAATG
GGTTGCCAGCGCGATTTTCGCACCACAATAGTGACAGTTTCGTGATAGGTGAAACAGGTTTCGGCACAGGCCTTAACTTT
CTGGCGACAATGAAAGCCTTCCTGGAACAGGCACCCCAAACCGGCAACGGCGCCCGTCTGCACTTCATCAGTTTCGAAAA
ATTCCCCCTCACCCGTGACGATCTTCACAAGGCGCTGACCGCCTGGCCCGAGCTTGCCCCCTTCAGCCAGGCACTCATAG
CACAGTGGCCACTGCCCGTCCCTGGCTGCCATCGTCTGCACTTTGCGGGCGGCCGCATTCGACTCGATCTCTGGCTGGGG
GACATCAAGGAGATGCTGCCGCAAGTACCTCATGGTGAACAGGGGCTGGTGGATACCTGGTATCTGGATGGATTCGCACC
GGCAAAAAATCCCGAGATGTGGACGCAAGACTTGTTCGACGGCCTGGCCCGCCTTGCTCGTCCCGGTGCAACCCTCTCCA
CCTTTACCAGCGCCGGCTTCGTGCGTCGTGGCCTGATTGCCGCCGGCTTTGCCATGAAGAAAGTCAAGGGCCATGGCAGC
AAGTGGGCAATGCTGGCAGGCGAGCGGGTAGACAAGAAACCACAGCGCACCATTGTCCCCTGGTATGCCAGACCGGCGGG
GCGAGACGGCGAGGTAGTGATCATCGGCGGCGGCATCGCCTCGGCCATGACGGCGCTCTCTCTGGTAGAGCGGGGACGCA
AGGTCACTCTGCTTTGCGAGGACCATGAACTGGCGACCGGTGCCTCCGGCAATCGACAGGGGGCACTCTATCCCCTGCTC
AACGGCGAGCACGATGCCTTGTCACGCTTTTATTCACTGGCCTTCGGCTATGCACGCCAACGGCTGCTGAGCCTGGCCAA
GCACCATCCCATCGCCTTTGATTTATGCGGTGTGGTGCAGCTTGGCTATGACGACAAATCCACCGCCAAGCTGGCCAAGA
TGCAACAAGGCCCCTTCCCGCACGCGTTGATGCGCCCGCTGACGCCCGTTGAAGCCGAACACGAATGTGGTCTGCCCTCG
GGCCATGCCGGGGTCAGCTATCCGTTGGGGGGCTGGCTCTGTCCGGCCGACCTCACCCGCGCAGCAATAAAGGAGGCGCA
GGCAAGCGGTCTGCTGCAAGTGGAGTACGATTGCCCTGTCACGGCTATTCATGAGCAAGTGGATGGCTGGCTGGTTGCAA
GCCGTGAGGGTCGTCAATGGCAGGCCCCCAATCTGGTAGTGGCTGCCGGTCATCAGTTGCCAGCCCTGAGCCCCTTTGCC
GAGCTGCCACTCTATCCGGTGCGCGGTCAAGTGAGCCATGTGCCGACTACCGCAACCCTGAGCCAGCTCAAGACGGTGCT
CTGTTACGATGGCTATCTCACGCCAGCCCATAATGCAGAACACTGCATCGGCGCCAGCTATGGCCGCAATCAGAGCACAC
AGGCATTCAGCGCAGAAGAACAAGCTCAGAACCAGGCTCGGCTGCAAGCCTGCCTGCCGGACCAGGCCTGGCCAGCAGAG
GTGGATGTAAGCAGCAATGAGGCCAGGGTCGGCGTGCGCTGCGCCAGCCGGGATCACCTGCCGGTGGCCGGGCCAATCGC
CAGGTTGGCAGGGTTGGCGGATCACTACGCCCGGCTTCAGCGTGATCAACAAAACCCGGCGCCGCTGCCGCTTCATCCGG
GCCTCTACGTGCTGGGGGCACTCGGTTCGCGGGGCCTGTGCTCGGCGCCTCTGTGCGGCGAGTTGATCGCCAGCGAGATC
TGCGGCGATCCCTTGCCACTGGCCACCGACCTGCTGGAAGCGCTTCATCCTGCCCGCTACTGGATACGCAAACTGCTCAA
GGGCAAGCCCCTCAATTAA

Upstream 100 bases:

>100_bases
CTCTTCTCATTAATTCATCTCGTCTGGAAAAACTGGCGCCAAGTCTACCCGCTTTCGGCTGCCAAACTGGTCTGCTTTCA
CGTAGAATGCCCGCGTTCTT

Downstream 100 bases:

>100_bases
CCCATTGGTGCAGCTGACGGCTTGAATACAATAAAAACGGGCTCCCCGATGGGAGCCCGTTTTATCGCTTGTCATGCATG
TCGATGTGCTAGATGCCTTT

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MTRAEGNVSQTSLHHARLDWNEAGTPVSSDFGDVYFSNDNGLSETRYVFLQQNGLPARFSHHNSDSFVIGETGFGTGLNF
LATMKAFLEQAPQTGNGARLHFISFEKFPLTRDDLHKALTAWPELAPFSQALIAQWPLPVPGCHRLHFAGGRIRLDLWLG
DIKEMLPQVPHGEQGLVDTWYLDGFAPAKNPEMWTQDLFDGLARLARPGATLSTFTSAGFVRRGLIAAGFAMKKVKGHGS
KWAMLAGERVDKKPQRTIVPWYARPAGRDGEVVIIGGGIASAMTALSLVERGRKVTLLCEDHELATGASGNRQGALYPLL
NGEHDALSRFYSLAFGYARQRLLSLAKHHPIAFDLCGVVQLGYDDKSTAKLAKMQQGPFPHALMRPLTPVEAEHECGLPS
GHAGVSYPLGGWLCPADLTRAAIKEAQASGLLQVEYDCPVTAIHEQVDGWLVASREGRQWQAPNLVVAAGHQLPALSPFA
ELPLYPVRGQVSHVPTTATLSQLKTVLCYDGYLTPAHNAEHCIGASYGRNQSTQAFSAEEQAQNQARLQACLPDQAWPAE
VDVSSNEARVGVRCASRDHLPVAGPIARLAGLADHYARLQRDQQNPAPLPLHPGLYVLGALGSRGLCSAPLCGELIASEI
CGDPLPLATDLLEALHPARYWIRKLLKGKPLN

Sequences:

>Translated_672_residues
MTRAEGNVSQTSLHHARLDWNEAGTPVSSDFGDVYFSNDNGLSETRYVFLQQNGLPARFSHHNSDSFVIGETGFGTGLNF
LATMKAFLEQAPQTGNGARLHFISFEKFPLTRDDLHKALTAWPELAPFSQALIAQWPLPVPGCHRLHFAGGRIRLDLWLG
DIKEMLPQVPHGEQGLVDTWYLDGFAPAKNPEMWTQDLFDGLARLARPGATLSTFTSAGFVRRGLIAAGFAMKKVKGHGS
KWAMLAGERVDKKPQRTIVPWYARPAGRDGEVVIIGGGIASAMTALSLVERGRKVTLLCEDHELATGASGNRQGALYPLL
NGEHDALSRFYSLAFGYARQRLLSLAKHHPIAFDLCGVVQLGYDDKSTAKLAKMQQGPFPHALMRPLTPVEAEHECGLPS
GHAGVSYPLGGWLCPADLTRAAIKEAQASGLLQVEYDCPVTAIHEQVDGWLVASREGRQWQAPNLVVAAGHQLPALSPFA
ELPLYPVRGQVSHVPTTATLSQLKTVLCYDGYLTPAHNAEHCIGASYGRNQSTQAFSAEEQAQNQARLQACLPDQAWPAE
VDVSSNEARVGVRCASRDHLPVAGPIARLAGLADHYARLQRDQQNPAPLPLHPGLYVLGALGSRGLCSAPLCGELIASEI
CGDPLPLATDLLEALHPARYWIRKLLKGKPLN
>Mature_671_residues
TRAEGNVSQTSLHHARLDWNEAGTPVSSDFGDVYFSNDNGLSETRYVFLQQNGLPARFSHHNSDSFVIGETGFGTGLNFL
ATMKAFLEQAPQTGNGARLHFISFEKFPLTRDDLHKALTAWPELAPFSQALIAQWPLPVPGCHRLHFAGGRIRLDLWLGD
IKEMLPQVPHGEQGLVDTWYLDGFAPAKNPEMWTQDLFDGLARLARPGATLSTFTSAGFVRRGLIAAGFAMKKVKGHGSK
WAMLAGERVDKKPQRTIVPWYARPAGRDGEVVIIGGGIASAMTALSLVERGRKVTLLCEDHELATGASGNRQGALYPLLN
GEHDALSRFYSLAFGYARQRLLSLAKHHPIAFDLCGVVQLGYDDKSTAKLAKMQQGPFPHALMRPLTPVEAEHECGLPSG
HAGVSYPLGGWLCPADLTRAAIKEAQASGLLQVEYDCPVTAIHEQVDGWLVASREGRQWQAPNLVVAAGHQLPALSPFAE
LPLYPVRGQVSHVPTTATLSQLKTVLCYDGYLTPAHNAEHCIGASYGRNQSTQAFSAEEQAQNQARLQACLPDQAWPAEV
DVSSNEARVGVRCASRDHLPVAGPIARLAGLADHYARLQRDQQNPAPLPLHPGLYVLGALGSRGLCSAPLCGELIASEIC
GDPLPLATDLLEALHPARYWIRKLLKGKPLN

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=667, Percent_Identity=50.9745127436282, Blast_Score=642, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_AERS4 (A4SNE3)

Other databases:

- EMBL:   CP000644
- RefSeq:   YP_001142163.1
- ProteinModelPortal:   A4SNE3
- SMR:   A4SNE3
- STRING:   A4SNE3
- GeneID:   4994823
- GenomeReviews:   CP000644_GR
- KEGG:   asa:ASA_2366
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- PhylomeDB:   A4SNE3
- ProtClustDB:   PRK01747
- BioCyc:   ASAL382245:ASA_2366-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 72937; Mature: 72806

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAEGNVSQTSLHHARLDWNEAGTPVSSDFGDVYFSNDNGLSETRYVFLQQNGLPARFS
CCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCEEC
HHNSDSFVIGETGFGTGLNFLATMKAFLEQAPQTGNGARLHFISFEKFPLTRDDLHKALT
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
AWPELAPFSQALIAQWPLPVPGCHRLHFAGGRIRLDLWLGDIKEMLPQVPHGEQGLVDTW
HCCCCCHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEHHHHHHHHCCCCCCCCCCEEEE
YLDGFAPAKNPEMWTQDLFDGLARLARPGATLSTFTSAGFVRRGLIAAGFAMKKVKGHGS
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KWAMLAGERVDKKPQRTIVPWYARPAGRDGEVVIIGGGIASAMTALSLVERGRKVTLLCE
EEEEEECCCCCCCCCCEEECEECCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEC
DHELATGASGNRQGALYPLLNGEHDALSRFYSLAFGYARQRLLSLAKHHPIAFDLCGVVQ
CCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
LGYDDKSTAKLAKMQQGPFPHALMRPLTPVEAEHECGLPSGHAGVSYPLGGWLCPADLTR
ECCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHH
AAIKEAQASGLLQVEYDCPVTAIHEQVDGWLVASREGRQWQAPNLVVAAGHQLPALSPFA
HHHHHHHHCCCEEEEECCCHHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCCCCCCCHH
ELPLYPVRGQVSHVPTTATLSQLKTVLCYDGYLTPAHNAEHCIGASYGRNQSTQAFSAEE
HCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHCCCCCCCCCCCHHHCHHH
QAQNQARLQACLPDQAWPAEVDVSSNEARVGVRCASRDHLPVAGPIARLAGLADHYARLQ
HHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
RDQQNPAPLPLHPGLYVLGALGSRGLCSAPLCGELIASEICGDPLPLATDLLEALHPARY
HCCCCCCCCCCCCCHHHEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
WIRKLLKGKPLN
HHHHHHCCCCCC
>Mature Secondary Structure 
TRAEGNVSQTSLHHARLDWNEAGTPVSSDFGDVYFSNDNGLSETRYVFLQQNGLPARFS
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCEEC
HHNSDSFVIGETGFGTGLNFLATMKAFLEQAPQTGNGARLHFISFEKFPLTRDDLHKALT
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
AWPELAPFSQALIAQWPLPVPGCHRLHFAGGRIRLDLWLGDIKEMLPQVPHGEQGLVDTW
HCCCCCHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEHHHHHHHHCCCCCCCCCCEEEE
YLDGFAPAKNPEMWTQDLFDGLARLARPGATLSTFTSAGFVRRGLIAAGFAMKKVKGHGS
EECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KWAMLAGERVDKKPQRTIVPWYARPAGRDGEVVIIGGGIASAMTALSLVERGRKVTLLCE
EEEEEECCCCCCCCCCEEECEECCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEC
DHELATGASGNRQGALYPLLNGEHDALSRFYSLAFGYARQRLLSLAKHHPIAFDLCGVVQ
CCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
LGYDDKSTAKLAKMQQGPFPHALMRPLTPVEAEHECGLPSGHAGVSYPLGGWLCPADLTR
ECCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHH
AAIKEAQASGLLQVEYDCPVTAIHEQVDGWLVASREGRQWQAPNLVVAAGHQLPALSPFA
HHHHHHHHCCCEEEEECCCHHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCCCCCCCHH
ELPLYPVRGQVSHVPTTATLSQLKTVLCYDGYLTPAHNAEHCIGASYGRNQSTQAFSAEE
HCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHCCCCCCCCCCCHHHCHHH
QAQNQARLQACLPDQAWPAEVDVSSNEARVGVRCASRDHLPVAGPIARLAGLADHYARLQ
HHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
RDQQNPAPLPLHPGLYVLGALGSRGLCSAPLCGELIASEICGDPLPLATDLLEALHPARY
HCCCCCCCCCCCCCHHHEECCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
WIRKLLKGKPLN
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA