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The map label for this gene is nrfF [H]

Identifier: 145299282

GI number: 145299282

Start: 2467425

End: 2467994

Strand: Direct

Name: nrfF [H]

Synonym: ASA_2326

Alternate gene names: 145299282

Gene position: 2467425-2467994 (Clockwise)

Preceding gene: 145299281

Following gene: 145299283

Centisome position: 52.47

GC content: 65.79

Gene sequence:

>570_bases
ATGAGCCCGCGCCTGCGTCTTTTTCTCCCCTTGCTGCTGACTCTGGGCCTCGGCACCCTGCTTTGGCTGGGGCTTGCCAA
CAATCCCTACCGCCAGGACGAGGCGACCCTGGGGCGTACCCTGCCCCAGTGGCAGAGCGAAAACCTGCTGGATGGGGGGC
TTGTCCACACCGACCAGCTCAAGGGAGAGCCCTTTGTGCTCAACGTCTGGGCCAGCTGGTGCCCCAACTGCCGCGCCGAG
CACCCGCTGCTCGCCGAGCTGGCCAGCACGGTTCCCCTCTATGGGCTCAACTACAGAGACAAGGGCGAAGCGGCGCGTGC
CTGGCTTGGTGAGGCAGGCAACCCCTATCGCGCCGTGCTGGCGGACTCGGACGGCAAGTTGGCGCTGGAGCTCGGCGTCT
ACGGCACGCCCGAAACCTATCTGTTTGCCGCCGATGGCACCCTGCTCACCCGCCACACGGGTCTCTTGACCACGGATATC
TGGCAGCGCCAGTTTGTGCCAAAGCTCAAGGCCGCCCGCGCCACGACCCTGACATCCTCAACGACCACCGGGCAGGAGGG
CAAACCATGA

Upstream 100 bases:

>100_bases
TGATGATGGCAGGAGGTGCCCTGCGTTTGTCCAGGCGCAAGCCGTTGCTGGTATCCCGTCCAGCCAAGATCCCGCAGCCC
CTGCGCGAACAGGAGGCACC

Downstream 100 bases:

>100_bases
AGACCCCTGTCATCCTCTGCCCCCTGCTGCTTGTACTCGGTATGAGCGTGGGCGCCCTGGCCAGCGGGGTCGATACCTAT
CAGTTCCAGCACCCGGAGCG

Product: formate-dependent nitrite reductase complex subunit NrfF

Products: NA

Alternate protein names: Cytochrome c biogenesis protein NrfX [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MSPRLRLFLPLLLTLGLGTLLWLGLANNPYRQDEATLGRTLPQWQSENLLDGGLVHTDQLKGEPFVLNVWASWCPNCRAE
HPLLAELASTVPLYGLNYRDKGEAARAWLGEAGNPYRAVLADSDGKLALELGVYGTPETYLFAADGTLLTRHTGLLTTDI
WQRQFVPKLKAARATTLTSSTTTGQEGKP

Sequences:

>Translated_189_residues
MSPRLRLFLPLLLTLGLGTLLWLGLANNPYRQDEATLGRTLPQWQSENLLDGGLVHTDQLKGEPFVLNVWASWCPNCRAE
HPLLAELASTVPLYGLNYRDKGEAARAWLGEAGNPYRAVLADSDGKLALELGVYGTPETYLFAADGTLLTRHTGLLTTDI
WQRQFVPKLKAARATTLTSSTTTGQEGKP
>Mature_188_residues
SPRLRLFLPLLLTLGLGTLLWLGLANNPYRQDEATLGRTLPQWQSENLLDGGLVHTDQLKGEPFVLNVWASWCPNCRAEH
PLLAELASTVPLYGLNYRDKGEAARAWLGEAGNPYRAVLADSDGKLALELGVYGTPETYLFAADGTLLTRHTGLLTTDIW
QRQFVPKLKAARATTLTSSTTTGQEGKP

Specific function: Could be involved in disulfide bond formation. Could catalyzes a late, reductive step in the assembly of periplasmic NrfA c-type cytochrome, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1788523, Length=172, Percent_Identity=40.6976744186046, Blast_Score=130, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: NA

Molecular weight: Translated: 20730; Mature: 20599

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRLRLFLPLLLTLGLGTLLWLGLANNPYRQDEATLGRTLPQWQSENLLDGGLVHTDQL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCCC
KGEPFVLNVWASWCPNCRAEHPLLAELASTVPLYGLNYRDKGEAARAWLGEAGNPYRAVL
CCCCEEEEEHHHHCCCCCCCCCHHHHHHHHCCEECCCCCCCCHHHHHHHCCCCCCEEEEE
ADSDGKLALELGVYGTPETYLFAADGTLLTRHTGLLTTDIWQRQFVPKLKAARATTLTSS
ECCCCCEEEEECCCCCCCEEEEECCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHEECCC
TTTGQEGKP
CCCCCCCCC
>Mature Secondary Structure 
SPRLRLFLPLLLTLGLGTLLWLGLANNPYRQDEATLGRTLPQWQSENLLDGGLVHTDQL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCCC
KGEPFVLNVWASWCPNCRAEHPLLAELASTVPLYGLNYRDKGEAARAWLGEAGNPYRAVL
CCCCEEEEEHHHHCCCCCCCCCHHHHHHHHCCEECCCCCCCCHHHHHHHCCCCCCEEEEE
ADSDGKLALELGVYGTPETYLFAADGTLLTRHTGLLTTDIWQRQFVPKLKAARATTLTSS
ECCCCCEEEEECCCCCCCEEEEECCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHEECCC
TTTGQEGKP
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]