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The map label for this gene is phaJ

Identifier: 145299239

GI number: 145299239

Start: 2407194

End: 2407604

Strand: Direct

Name: phaJ

Synonym: ASA_2281

Alternate gene names: NA

Gene position: 2407194-2407604 (Clockwise)

Preceding gene: 145299238

Following gene: 145299240

Centisome position: 51.19

GC content: 64.48

Gene sequence:

>411_bases
ATGAACACACTCCCCCTTGAAATTGGGCAAAAAGCCAGCCTGACCAAACGGTTCGGCGCAGCCGAAGTCGAGGCCTTCGC
CGGCTTGTCGGAGGATTTCAATCCCCTGCACCTGGACTCGGCTTTTGCCGCTACCACGCCATTCGAGCGCCCCATAGTGC
ACGGCATGCTGCTGGCCAGCCTCTTCTCCGGGCTGCTGGGCCAGCAGCTGCCGGGCAAGGGCACCGTCTACCTGGGGCAG
AGCCTCTCCTTCAAGCAACCGGTGTTTGTGGGGGATGAGGTGACGGCCGAGGTGGAGATCATCGCCATGCGCAGTGACAA
GCCCATCATCACCCTGGCCACCCGCATCCTGGCCGCTGGCGGCGCCCTCGCCCTCGCCGTGACCGGAGAAGCCGTGGTGA
AGGTGGGCTGA

Upstream 100 bases:

>100_bases
CCGCCCGCATCCCGAGCGAGGGACTGGAGGCGGCCCCCGGCGGTTATGTCAAGGTGCGGCTTAACCCTGTCTTTGCCAAC
ACCCCGGTGGAGGAGCCCGC

Downstream 100 bases:

>100_bases
CTTCAATACTTGCACTCCCTGAGTCAGCCCGGCACCCGCCGGGCTGACGCTTGTCGTTCACACTGTCACGCTGCTTTTAT
GATGCCTTCTCTACCCCGCC

Product: enoyl-CoA hydrotase

Products: crotonoyl-CoA; H2O

Alternate protein names: MaoC Domain Protein Dehydratase; MaoC-Like Dehydratase; Acyl Dehydratase; Phosphate Acetyltransferase; MaoC Domain Protein; MaoC Family Protein; Phosphate Butyryltransferase; Dehydrogenase; 3-Hydroxybutyryl-CoA Dehydratase; MaoC Domain-Containing Protein Dehydratase; MaoC Domain-Containing Protein; (De)Hydratase; Enoyl-CoA Hydratase; MaoC-Like Domain-Containing Protein; Maoc Domain Protein Dehydratase; Phosphate Acetyl/Butaryl Transferase; MaoC Like Domain Protein; MaoC-Like Domain Protein; MaoC Family Protein/Phosphate Acetyl/Butaryl Transferase; 3-Re Enoyl-CoA Hydratase PhaJ; MaoC Like Domain; Oxidoreductase; Enoyl-CoA Hydratase R-Specific; MaoC Family Dehydratase; Enoyl-CoA Hydrotase; MaoC-Like Acyl Dehydratase; MaoC-Related Acyl Dehydratase; (R)-Specific Enoyl-CoA Hydratase/MaoC Family Protein

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MNTLPLEIGQKASLTKRFGAAEVEAFAGLSEDFNPLHLDSAFAATTPFERPIVHGMLLASLFSGLLGQQLPGKGTVYLGQ
SLSFKQPVFVGDEVTAEVEIIAMRSDKPIITLATRILAAGGALALAVTGEAVVKVG

Sequences:

>Translated_136_residues
MNTLPLEIGQKASLTKRFGAAEVEAFAGLSEDFNPLHLDSAFAATTPFERPIVHGMLLASLFSGLLGQQLPGKGTVYLGQ
SLSFKQPVFVGDEVTAEVEIIAMRSDKPIITLATRILAAGGALALAVTGEAVVKVG
>Mature_136_residues
MNTLPLEIGQKASLTKRFGAAEVEAFAGLSEDFNPLHLDSAFAATTPFERPIVHGMLLASLFSGLLGQQLPGKGTVYLGQ
SLSFKQPVFVGDEVTAEVEIIAMRSDKPIITLATRILAAGGALALAVTGEAVVKVG

Specific function: Unknown

COG id: COG2030

COG function: function code I; Acyl dehydratase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.55

Molecular weight: Translated: 14196; Mature: 14196

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLPLEIGQKASLTKRFGAAEVEAFAGLSEDFNPLHLDSAFAATTPFERPIVHGMLLAS
CCCCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCEEECCCCHHCCCCCCCHHHHHHHHHH
LFSGLLGQQLPGKGTVYLGQSLSFKQPVFVGDEVTAEVEIIAMRSDKPIITLATRILAAG
HHHHHHCCCCCCCCEEEECCCCCCCCCEEECCCCEEEEEEEEEECCCCHHHHHHHHHHCC
GALALAVTGEAVVKVG
CEEEEEEECCEEEEEC
>Mature Secondary Structure
MNTLPLEIGQKASLTKRFGAAEVEAFAGLSEDFNPLHLDSAFAATTPFERPIVHGMLLAS
CCCCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCEEECCCCHHCCCCCCCHHHHHHHHHH
LFSGLLGQQLPGKGTVYLGQSLSFKQPVFVGDEVTAEVEIIAMRSDKPIITLATRILAAG
HHHHHHCCCCCCCCEEEECCCCCCCCCEEECCCCEEEEEEEEEECCCCHHHHHHHHHHCC
GALALAVTGEAVVKVG
CEEEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxybutanoyl-CoA

Specific reaction: (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA