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The map label for this gene is phaC [H]

Identifier: 145299238

GI number: 145299238

Start: 2405413

End: 2407197

Strand: Direct

Name: phaC [H]

Synonym: ASA_2280

Alternate gene names: 145299238

Gene position: 2405413-2407197 (Clockwise)

Preceding gene: 145299237

Following gene: 145299239

Centisome position: 51.15

GC content: 62.91

Gene sequence:

>1785_bases
ATGAGCCAATCATCTTACGGCCCGCTGTTCGAGGCCCTGGCCCACTATAACGACAAGTTGCTGGACATGGCCAAGGCACA
GACAGAGCGCACGGCGCAGGCGCTGTTGCAGACCAATCTGGACGATCTGGGCCAGGTACTGGAACAGGGCACTCAGCAAC
CCTGGCAACTGATCAATGCCCAGATGAACTGGTGGCAGGATCAGCTCAAACTGATGCAGCATACCCTGCTCAAAAGCGCA
GGCCAGGAGAGCGAGCCGGTGATCACGCCGGAGCGCAGCGATCGCCGCTTCAAGGACGACGCCTGGCGTGACCAGCCCCT
TTATGACTACCTCAAGCAATCCTATCTGCTGACCGCCAGGCACCTGCTGGCCTCGGTAGATGCGCTGGAAGGGGTGCCCC
AGAAGAGCCGGGAGCGACTGCGCTTTTTTACCCGTCAATACCTCAATGCCATGGCGCCCAGCAACTTTCTGGCCACCAAC
CCCGAGTTGCTCAGGCTCACCCTGGAATCCGACGGCCAGAATCTGGTGCGCGGACTGGCCCTGCTGGCCGAGGATCTGGA
GCGCAGCGCCGATCAGCTCAACATCCGCCTGACCGACGAGTCCGCCTTCGAACTGGGGCGGGATCTGGCCTTGACGCCGG
GCCGGGTGGTGCAACGCACCGAGCTCTACGAGCTTATCCAGTACAGCCCGACCACGGAAACCGTGGCCAGGACGCCGGTG
CTGATAGTGCCGCCCTTCATCAACAAGTACTACATCATGGACATGCGGCCCCAGAACTCTCTGGTCGCCTGGCTGGTCGC
CCAGGGCCAGACGGTATTCATGATCTCCTGGCGCAACCCGGGGGTGGAGCAGGCCAATATCGATCTCGACGACTATGTGG
TCGATGGGGTGATCGCTGCGCTGGACGGCGTGGAAGCGGCCACCGGCGAGCGTGAGGTGCACGGTATCGGTTACTGCATC
GGCGGTACCGCCCTGTCACTCGCCATGGGCTGGCTGGCGGCGCGGCGCCAGAAACAGCGGGGGCGCTCCGCCACCCTGTT
TACCACACTGCTCGATTTCTCCCAGCCGGGGGAGCTTGGCATCTTCATCCACGAGCCCATCATAGCGGCGCTGGAGGCCC
AGAATGAGGCCAAGGGCATCATGGATGGACGCCAGCTGGCGGTCTCCTTCAGCCTGCTGCGGGAAAACAGCCTCTACTGG
AATTACTACATCGACAGCTACCTCAAGGGCCAGAGCCCGGTGGCGTTCGATCTGCTGCACTGGAACAGCGACAGCACCAA
CGTGGCGGGCAAGACCCACAACAGTCTGCTGCGTCGTCTCTATCTGGAGAACCAGCTGGTCAAGGGGGAGCTCAAGATCC
GCAACACCCGCATCGACCTGGCCAAGGTGAAGACGCCTGTGCTGCTGGTCTCGGCCATGGACGATCACATCGCCCTCTGG
CAGGGCACCTGGCAGGGCATGAAGCTGTTTGGCGACGAGCAGCGCTTCATCCTGGCCGAATCGGGCCACATTGCCGGCAT
CATCAACCCGCCTGCGGCCAACAAGTACGGCTTCTGGCACAGCGATGCGAGCAGTAACACCCCGGACGCCTGGCTTGAGC
AGGCAACCCATCAAAGCGGCTCCTGGTGGCCCGAGATGGCAGCCTTCATCAAGGGGCGTGAAGAGGAGGCCACGCCCGTG
CCCGCCCGCATCCCGAGCGAGGGACTGGAGGCGGCCCCCGGCGGTTATGTCAAGGTGCGGCTTAACCCTGTCTTTGCCAA
CACCCCGGTGGAGGAGCCCGCATGA

Upstream 100 bases:

>100_bases
GGCCTGATATCCCCCTGGCTGCCCGATGCGGGCAGCCACATCTCCCCATGACCCGACACGACAGGCTCGCCCTGTCACGG
GTGTGGGTGAAGGAGAGAAC

Downstream 100 bases:

>100_bases
ACACACTCCCCCTTGAAATTGGGCAAAAAGCCAGCCTGACCAAACGGTTCGGCGCAGCCGAAGTCGAGGCCTTCGCCGGC
TTGTCGGAGGATTTCAATCC

Product: poly(3-hydroxyalkanoate) polymerase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MSQSSYGPLFEALAHYNDKLLDMAKAQTERTAQALLQTNLDDLGQVLEQGTQQPWQLINAQMNWWQDQLKLMQHTLLKSA
GQESEPVITPERSDRRFKDDAWRDQPLYDYLKQSYLLTARHLLASVDALEGVPQKSRERLRFFTRQYLNAMAPSNFLATN
PELLRLTLESDGQNLVRGLALLAEDLERSADQLNIRLTDESAFELGRDLALTPGRVVQRTELYELIQYSPTTETVARTPV
LIVPPFINKYYIMDMRPQNSLVAWLVAQGQTVFMISWRNPGVEQANIDLDDYVVDGVIAALDGVEAATGEREVHGIGYCI
GGTALSLAMGWLAARRQKQRGRSATLFTTLLDFSQPGELGIFIHEPIIAALEAQNEAKGIMDGRQLAVSFSLLRENSLYW
NYYIDSYLKGQSPVAFDLLHWNSDSTNVAGKTHNSLLRRLYLENQLVKGELKIRNTRIDLAKVKTPVLLVSAMDDHIALW
QGTWQGMKLFGDEQRFILAESGHIAGIINPPAANKYGFWHSDASSNTPDAWLEQATHQSGSWWPEMAAFIKGREEEATPV
PARIPSEGLEAAPGGYVKVRLNPVFANTPVEEPA

Sequences:

>Translated_594_residues
MSQSSYGPLFEALAHYNDKLLDMAKAQTERTAQALLQTNLDDLGQVLEQGTQQPWQLINAQMNWWQDQLKLMQHTLLKSA
GQESEPVITPERSDRRFKDDAWRDQPLYDYLKQSYLLTARHLLASVDALEGVPQKSRERLRFFTRQYLNAMAPSNFLATN
PELLRLTLESDGQNLVRGLALLAEDLERSADQLNIRLTDESAFELGRDLALTPGRVVQRTELYELIQYSPTTETVARTPV
LIVPPFINKYYIMDMRPQNSLVAWLVAQGQTVFMISWRNPGVEQANIDLDDYVVDGVIAALDGVEAATGEREVHGIGYCI
GGTALSLAMGWLAARRQKQRGRSATLFTTLLDFSQPGELGIFIHEPIIAALEAQNEAKGIMDGRQLAVSFSLLRENSLYW
NYYIDSYLKGQSPVAFDLLHWNSDSTNVAGKTHNSLLRRLYLENQLVKGELKIRNTRIDLAKVKTPVLLVSAMDDHIALW
QGTWQGMKLFGDEQRFILAESGHIAGIINPPAANKYGFWHSDASSNTPDAWLEQATHQSGSWWPEMAAFIKGREEEATPV
PARIPSEGLEAAPGGYVKVRLNPVFANTPVEEPA
>Mature_593_residues
SQSSYGPLFEALAHYNDKLLDMAKAQTERTAQALLQTNLDDLGQVLEQGTQQPWQLINAQMNWWQDQLKLMQHTLLKSAG
QESEPVITPERSDRRFKDDAWRDQPLYDYLKQSYLLTARHLLASVDALEGVPQKSRERLRFFTRQYLNAMAPSNFLATNP
ELLRLTLESDGQNLVRGLALLAEDLERSADQLNIRLTDESAFELGRDLALTPGRVVQRTELYELIQYSPTTETVARTPVL
IVPPFINKYYIMDMRPQNSLVAWLVAQGQTVFMISWRNPGVEQANIDLDDYVVDGVIAALDGVEAATGEREVHGIGYCIG
GTALSLAMGWLAARRQKQRGRSATLFTTLLDFSQPGELGIFIHEPIIAALEAQNEAKGIMDGRQLAVSFSLLRENSLYWN
YYIDSYLKGQSPVAFDLLHWNSDSTNVAGKTHNSLLRRLYLENQLVKGELKIRNTRIDLAKVKTPVLLVSAMDDHIALWQ
GTWQGMKLFGDEQRFILAESGHIAGIINPPAANKYGFWHSDASSNTPDAWLEQATHQSGSWWPEMAAFIKGREEEATPVP
ARIPSEGLEAAPGGYVKVRLNPVFANTPVEEPA

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 66680; Mature: 66549

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSSYGPLFEALAHYNDKLLDMAKAQTERTAQALLQTNLDDLGQVLEQGTQQPWQLINA
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHH
QMNWWQDQLKLMQHTLLKSAGQESEPVITPERSDRRFKDDAWRDQPLYDYLKQSYLLTAR
HHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
HLLASVDALEGVPQKSRERLRFFTRQYLNAMAPSNFLATNPELLRLTLESDGQNLVRGLA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHH
LLAEDLERSADQLNIRLTDESAFELGRDLALTPGRVVQRTELYELIQYSPTTETVARTPV
HHHHHHHCCHHHEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCE
LIVPPFINKYYIMDMRPQNSLVAWLVAQGQTVFMISWRNPGVEQANIDLDDYVVDGVIAA
EEECCCCCCEEEEEECCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
LDGVEAATGEREVHGIGYCIGGTALSLAMGWLAARRQKQRGRSATLFTTLLDFSQPGELG
HCCCHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEE
IFIHEPIIAALEAQNEAKGIMDGRQLAVSFSLLRENSLYWNYYIDSYLKGQSPVAFDLLH
EEECCHHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCEEEEEEE
WNSDSTNVAGKTHNSLLRRLYLENQLVKGELKIRNTRIDLAKVKTPVLLVSAMDDHIALW
ECCCCCCCCCCHHHHHHHHHHHHHHHEECEEEEEECEEEHHHHCCCEEEEEECCCCEEEE
QGTWQGMKLFGDEQRFILAESGHIAGIINPPAANKYGFWHSDASSNTPDAWLEQATHQSG
CCCCCCHHEECCCCEEEEEECCCEEEEECCCCCCCCCEEECCCCCCCCHHHHHHHHCCCC
SWWPEMAAFIKGREEEATPVPARIPSEGLEAAPGGYVKVRLNPVFANTPVEEPA
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
SQSSYGPLFEALAHYNDKLLDMAKAQTERTAQALLQTNLDDLGQVLEQGTQQPWQLINA
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHH
QMNWWQDQLKLMQHTLLKSAGQESEPVITPERSDRRFKDDAWRDQPLYDYLKQSYLLTAR
HHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
HLLASVDALEGVPQKSRERLRFFTRQYLNAMAPSNFLATNPELLRLTLESDGQNLVRGLA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHH
LLAEDLERSADQLNIRLTDESAFELGRDLALTPGRVVQRTELYELIQYSPTTETVARTPV
HHHHHHHCCHHHEEEEECCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCE
LIVPPFINKYYIMDMRPQNSLVAWLVAQGQTVFMISWRNPGVEQANIDLDDYVVDGVIAA
EEECCCCCCEEEEEECCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
LDGVEAATGEREVHGIGYCIGGTALSLAMGWLAARRQKQRGRSATLFTTLLDFSQPGELG
HCCCHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEE
IFIHEPIIAALEAQNEAKGIMDGRQLAVSFSLLRENSLYWNYYIDSYLKGQSPVAFDLLH
EEECCHHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCEEEEEEE
WNSDSTNVAGKTHNSLLRRLYLENQLVKGELKIRNTRIDLAKVKTPVLLVSAMDDHIALW
ECCCCCCCCCCHHHHHHHHHHHHHHHEECEEEEEECEEEHHHHCCCEEEEEECCCCEEEE
QGTWQGMKLFGDEQRFILAESGHIAGIINPPAANKYGFWHSDASSNTPDAWLEQATHQSG
CCCCCCHHEECCCCEEEEEECCCEEEEECCCCCCCCCEEECCCCCCCCHHHHHHHHCCCC
SWWPEMAAFIKGREEEATPVPARIPSEGLEAAPGGYVKVRLNPVFANTPVEEPA
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]