Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 145297090

GI number: 145297090

Start: 3310641

End: 3311312

Strand: Reverse

Name: gidB

Synonym: cgR_2986

Alternate gene names: 145297090

Gene position: 3311312-3310641 (Counterclockwise)

Preceding gene: 145297091

Following gene: 145297089

Centisome position: 99.91

GC content: 53.42

Gene sequence:

>672_bases
GTGTTGTACCGCTCGATATCTTGCCCGAAGGGGACTTTTTTCATGACCACGCCTCCAGCAGCCGCTGAAATTTTCGGGGA
CAACCTCGAAAAAGCCATTGCATATCATGAATCTTTGGCAACAGATGGATCCGTCCGCGGATTCATCGGCCCCCGTGAAG
TTCCCCGCCTGTGGGATCGCCACATTCTAAACTGTGGTGTCATCGGCGAAGCTATGGATGAGGGCATCTCTGTTGCAGAC
ATTGGTTCTGGTGCGGGACTGCCAGGAATTCCTCTTGCTATCGCACGCCCAGATCTCAACATCACTCTCATTGAGCCATT
GCTCAAGCGTTCGGTGTACCTCAACGAGGTAAAAGAAGCCCTGAACTTGGACAATGTCACCGTCATTCGTGGTCGTGCTG
AGGAAAAAGTGGTGCGCAAGCAGGTCGGATTGGTCGATATTGTCACCTCCCGAGCTGTTGCTCCACTGGGCAAGCTAGCG
ACCTGGTCGTTGCCGCTGGTGAAAATCGGTGGACGCATGGTGGCCATGAAAGGCTCCAGCGTTGAGGAAGAAATTGAGCG
TGACGCCAAGGAAATCCGCAAGGCTGGCGGCACTGACATTAAGGTTTATACCGTGGGTGAGGCGCTGTTGAGCGAGCCCA
CAACACTTATTTCAATCCGCAGGGAAAAGTAA

Upstream 100 bases:

>100_bases
TTTGCTGCCTGCAGCGCGCCATTTTAAGCGCTTGTCGACGCTAAAAAGCACCATTCGGCAGCCAACACTCAAGCTTCTTA
AGTTGAGTCGTTATAGTTGA

Downstream 100 bases:

>100_bases
CCATCCACTTTGGTGGAATGGGTACAGCGTTAAGCACTAATGTGGAATCGATGACGTGTGCATGAAGAATTCGCATGCAC
AGGAAGATAGGGATGGTTGA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MLYRSISCPKGTFFMTTPPAAAEIFGDNLEKAIAYHESLATDGSVRGFIGPREVPRLWDRHILNCGVIGEAMDEGISVAD
IGSGAGLPGIPLAIARPDLNITLIEPLLKRSVYLNEVKEALNLDNVTVIRGRAEEKVVRKQVGLVDIVTSRAVAPLGKLA
TWSLPLVKIGGRMVAMKGSSVEEEIERDAKEIRKAGGTDIKVYTVGEALLSEPTTLISIRREK

Sequences:

>Translated_223_residues
MLYRSISCPKGTFFMTTPPAAAEIFGDNLEKAIAYHESLATDGSVRGFIGPREVPRLWDRHILNCGVIGEAMDEGISVAD
IGSGAGLPGIPLAIARPDLNITLIEPLLKRSVYLNEVKEALNLDNVTVIRGRAEEKVVRKQVGLVDIVTSRAVAPLGKLA
TWSLPLVKIGGRMVAMKGSSVEEEIERDAKEIRKAGGTDIKVYTVGEALLSEPTTLISIRREK
>Mature_223_residues
MLYRSISCPKGTFFMTTPPAAAEIFGDNLEKAIAYHESLATDGSVRGFIGPREVPRLWDRHILNCGVIGEAMDEGISVAD
IGSGAGLPGIPLAIARPDLNITLIEPLLKRSVYLNEVKEALNLDNVTVIRGRAEEKVVRKQVGLVDIVTSRAVAPLGKLA
TWSLPLVKIGGRMVAMKGSSVEEEIERDAKEIRKAGGTDIKVYTVGEALLSEPTTLISIRREK

Specific function: Specifically methylates the N7 position of guanosine in position 518 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=155, Percent_Identity=34.8387096774194, Blast_Score=85, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_CORGB (A4QIE3)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139911.1
- ProteinModelPortal:   A4QIE3
- SMR:   A4QIE3
- STRING:   A4QIE3
- GeneID:   4992040
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2986
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   VPRLWER
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 24117; Mature: 24117

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYRSISCPKGTFFMTTPPAAAEIFGDNLEKAIAYHESLATDGSVRGFIGPREVPRLWDR
CCCCCCCCCCCEEEEECCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
HILNCGVIGEAMDEGISVADIGSGAGLPGIPLAIARPDLNITLIEPLLKRSVYLNEVKEA
HHHCCCCHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHH
LNLDNVTVIRGRAEEKVVRKQVGLVDIVTSRAVAPLGKLATWSLPLVKIGGRMVAMKGSS
HCCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHCCCCHHHHCCEEEEEECCC
VEEEIERDAKEIRKAGGTDIKVYTVGEALLSEPTTLISIRREK
HHHHHHHHHHHHHHCCCCEEEEEEECHHHHHCCCEEEEEECCC
>Mature Secondary Structure
MLYRSISCPKGTFFMTTPPAAAEIFGDNLEKAIAYHESLATDGSVRGFIGPREVPRLWDR
CCCCCCCCCCCEEEEECCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
HILNCGVIGEAMDEGISVADIGSGAGLPGIPLAIARPDLNITLIEPLLKRSVYLNEVKEA
HHHCCCCHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHH
LNLDNVTVIRGRAEEKVVRKQVGLVDIVTSRAVAPLGKLATWSLPLVKIGGRMVAMKGSS
HCCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHCCCCHHHHCCEEEEEECCC
VEEEIERDAKEIRKAGGTDIKVYTVGEALLSEPTTLISIRREK
HHHHHHHHHHHHHHCCCCEEEEEEECHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA