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The map label for this gene is liaS [H]

Identifier: 145296945

GI number: 145296945

Start: 3156946

End: 3158280

Strand: Reverse

Name: liaS [H]

Synonym: cgR_2845

Alternate gene names: 145296945

Gene position: 3158280-3156946 (Counterclockwise)

Preceding gene: 145296947

Following gene: 145296944

Centisome position: 95.3

GC content: 53.41

Gene sequence:

>1335_bases
ATGCAGTCAAGCCTAGATCGTGTGTCGGAAACCGGACGCAATGAGCTCGATGTTGAAACCCTTGTGAAGAAGGGGAATCA
ACCGGGCGCGATGAGCTATCGCAACAGTATCCACATTTTGACAGCCTCGCTGCTGGTCGTGGGGTTGGGTGCTTCCGCCC
GCCTGACGCTGCCGATGTTTGCGCTGTCGTGCGTGCTGTTGTTTGTGTGGGGTTTTCTGTACTTCTATGGATCAACCAAA
CGCGTAGATTTGAGCCACGGCATGCAGCTGGGCTGGCTGTTTGTGCTGACGTTGGTGTGGATTTTTATGGTGCCCATCGT
GCCGGTGTCCATTTATCTGCTGTTCCCGCTGTTTTTCCTCTATCTACAGGTGATGCCTGACGTGAGAGGCATTATTGCGA
TTTTGGGTGCGACAGCGATTGCGATTGCCAGCCAGTATTCCGTGGGGTTGACCTTTGGTGGTGTGATGGGTCCGGTGGTC
TCTGCGATCGTGACCGTGGCTATTGATTACGCGTTCCGCACGTTGTGGCGGGTGAATAATGAAAAGCAGGAATTGATTGA
TCAGTTGATTGAAACTCGCTCCCAGCTGGCGGTGACGGAACGAAATGCGGGTATCGCTGCGGAACGTCAACGTATTGCGC
ATGAAATTCACGACACGGTCGCCCAGGGACTCTCCTCCATTCAAATGCTGCTGCATGTCTCTGAACAGGAGATTCTCGTT
GCTGAGATGGAAGAGAAGCCAAAGGAGGCGATCGTGAAGAAGATGCGCCTTGCCCGACAAACAGCCTCCGACAATCTCAG
TGAGGCTCGCGCGATGATTGCGGCGTTGCAACCGGCAGCGCTGTCTAAAACCTCCTTGGAAGCAGCACTTCACCGCGTCA
CAGAACCGTTGCTGGGGATTAATTTTGTGATTTCCATCGACGGTGATGTTCGCCAACTGCCCATGAAAACTGAAGCCACC
CTTCTGCGAATTGCTCAAGGTGCGATCGGAAATGTGGCGAAACATTCAGAGGCGAAAAACTGCCACGTGACATTGACTTA
CGAAGACACAGAAGTGCGCCTTGATGTGGTTGATGACGGTGTGGGTTTTGAGCCTTCGGAAGTGTCCAGTACCCCCGCTG
GCCTTGGCCATATCGGCTTAACTGCATTGCAGCAGCGTGCGATGGAATTACACGGCGAAGTTATAGTGGAATCTGCATAT
GGGCAGGGTACTGCGGTATCTGCAGCATTGCCGGTGGAGCCACCAGAGGGGTTTGTCGGGGCGCCGGTTTTGGCAGATTC
GGACTCAAGTGCTACAGGCGAGGTTGAACTAAGTTCTCCAACTGATGATGAGTAA

Upstream 100 bases:

>100_bases
GGCTGCCTCGGTGGTGGTCTCTGGGGTTGCTTCAGGTTCCGCCGGGGTACAAGCGGTGAGCATGATGGAAGCAGCGAGGA
TAGTAGGTAATGTACGACGC

Downstream 100 bases:

>100_bases
GGCTAGACTAAAGTACGATTCATCTGCTCATCGATACTCTTGAAGGCGCATTTTCATTCGAAACGAAGTGCGCCATTGGG
AAGGACCTAGTTCAAACAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MQSSLDRVSETGRNELDVETLVKKGNQPGAMSYRNSIHILTASLLVVGLGASARLTLPMFALSCVLLFVWGFLYFYGSTK
RVDLSHGMQLGWLFVLTLVWIFMVPIVPVSIYLLFPLFFLYLQVMPDVRGIIAILGATAIAIASQYSVGLTFGGVMGPVV
SAIVTVAIDYAFRTLWRVNNEKQELIDQLIETRSQLAVTERNAGIAAERQRIAHEIHDTVAQGLSSIQMLLHVSEQEILV
AEMEEKPKEAIVKKMRLARQTASDNLSEARAMIAALQPAALSKTSLEAALHRVTEPLLGINFVISIDGDVRQLPMKTEAT
LLRIAQGAIGNVAKHSEAKNCHVTLTYEDTEVRLDVVDDGVGFEPSEVSSTPAGLGHIGLTALQQRAMELHGEVIVESAY
GQGTAVSAALPVEPPEGFVGAPVLADSDSSATGEVELSSPTDDE

Sequences:

>Translated_444_residues
MQSSLDRVSETGRNELDVETLVKKGNQPGAMSYRNSIHILTASLLVVGLGASARLTLPMFALSCVLLFVWGFLYFYGSTK
RVDLSHGMQLGWLFVLTLVWIFMVPIVPVSIYLLFPLFFLYLQVMPDVRGIIAILGATAIAIASQYSVGLTFGGVMGPVV
SAIVTVAIDYAFRTLWRVNNEKQELIDQLIETRSQLAVTERNAGIAAERQRIAHEIHDTVAQGLSSIQMLLHVSEQEILV
AEMEEKPKEAIVKKMRLARQTASDNLSEARAMIAALQPAALSKTSLEAALHRVTEPLLGINFVISIDGDVRQLPMKTEAT
LLRIAQGAIGNVAKHSEAKNCHVTLTYEDTEVRLDVVDDGVGFEPSEVSSTPAGLGHIGLTALQQRAMELHGEVIVESAY
GQGTAVSAALPVEPPEGFVGAPVLADSDSSATGEVELSSPTDDE
>Mature_444_residues
MQSSLDRVSETGRNELDVETLVKKGNQPGAMSYRNSIHILTASLLVVGLGASARLTLPMFALSCVLLFVWGFLYFYGSTK
RVDLSHGMQLGWLFVLTLVWIFMVPIVPVSIYLLFPLFFLYLQVMPDVRGIIAILGATAIAIASQYSVGLTFGGVMGPVV
SAIVTVAIDYAFRTLWRVNNEKQELIDQLIETRSQLAVTERNAGIAAERQRIAHEIHDTVAQGLSSIQMLLHVSEQEILV
AEMEEKPKEAIVKKMRLARQTASDNLSEARAMIAALQPAALSKTSLEAALHRVTEPLLGINFVISIDGDVRQLPMKTEAT
LLRIAQGAIGNVAKHSEAKNCHVTLTYEDTEVRLDVVDDGVGFEPSEVSSTPAGLGHIGLTALQQRAMELHGEVIVESAY
GQGTAVSAALPVEPPEGFVGAPVLADSDSSATGEVELSSPTDDE

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=238, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47977; Mature: 47977

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSSLDRVSETGRNELDVETLVKKGNQPGAMSYRNSIHILTASLLVVGLGASARLTLPMF
CCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCHHHHHH
ALSCVLLFVWGFLYFYGSTKRVDLSHGMQLGWLFVLTLVWIFMVPIVPVSIYLLFPLFFL
HHHHHHHHHHHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLQVMPDVRGIIAILGATAIAIASQYSVGLTFGGVMGPVVSAIVTVAIDYAFRTLWRVNN
HHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCC
EKQELIDQLIETRSQLAVTERNAGIAAERQRIAHEIHDTVAQGLSSIQMLLHVSEQEILV
HHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AEMEEKPKEAIVKKMRLARQTASDNLSEARAMIAALQPAALSKTSLEAALHRVTEPLLGI
HHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCE
NFVISIDGDVRQLPMKTEATLLRIAQGAIGNVAKHSEAKNCHVTLTYEDTEVRLDVVDDG
EEEEEECCCHHHCCCHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECCEEEEEEEECC
VGFEPSEVSSTPAGLGHIGLTALQQRAMELHGEVIVESAYGQGTAVSAALPVEPPEGFVG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCCCCC
APVLADSDSSATGEVELSSPTDDE
CCEEECCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MQSSLDRVSETGRNELDVETLVKKGNQPGAMSYRNSIHILTASLLVVGLGASARLTLPMF
CCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCHHHHHH
ALSCVLLFVWGFLYFYGSTKRVDLSHGMQLGWLFVLTLVWIFMVPIVPVSIYLLFPLFFL
HHHHHHHHHHHHHHHHCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLQVMPDVRGIIAILGATAIAIASQYSVGLTFGGVMGPVVSAIVTVAIDYAFRTLWRVNN
HHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCC
EKQELIDQLIETRSQLAVTERNAGIAAERQRIAHEIHDTVAQGLSSIQMLLHVSEQEILV
HHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AEMEEKPKEAIVKKMRLARQTASDNLSEARAMIAALQPAALSKTSLEAALHRVTEPLLGI
HHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCE
NFVISIDGDVRQLPMKTEATLLRIAQGAIGNVAKHSEAKNCHVTLTYEDTEVRLDVVDDG
EEEEEECCCHHHCCCHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEECCEEEEEEEECC
VGFEPSEVSSTPAGLGHIGLTALQQRAMELHGEVIVESAYGQGTAVSAALPVEPPEGFVG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCCCCC
APVLADSDSSATGEVELSSPTDDE
CCEEECCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]