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The map label for this gene is liaR [H]

Identifier: 145296944

GI number: 145296944

Start: 3156209

End: 3156847

Strand: Reverse

Name: liaR [H]

Synonym: cgR_2844

Alternate gene names: 145296944

Gene position: 3156847-3156209 (Counterclockwise)

Preceding gene: 145296945

Following gene: 145296942

Centisome position: 95.25

GC content: 60.88

Gene sequence:

>639_bases
ATGATTCGCGTGCTGCTTGCTGATGACCACGAAATCGTGAGGCTCGGACTCCGGGCTGTGCTGGAAAGCGCCGAGGACAT
TGAAGTGGTGGGCGAAGTCTCCACCGCCGAAGGTGCGGTGCAGGCAGCCCAAGAAGGCGGAATCGACGTCATCTTGATGG
ACCTCCGGTTCGGCCCCGGCGTCCAAGGAACCCAGGTATCCACCGGCGCAGACGCCACCGCAGCCATCAAGCGAAACATC
GATAACCCGCCAAAAGTCCTGGTCGTGACCAACTACGACACCGACGCAGACATCCTCGGCGCAATCGAAGCCGGCGCACT
GGGCTACCTGCTCAAAGACGCCCCACCGAGCGAACTCCTGGCAGCAGTACGATCCGCAGCAGAAGGCGATTCCACACTGT
CACCCATGGTTGCTAACCGCCTGATGACTCGCGTGCGTACCCCCAAAACCTCACTCACCCCACGTGAACTGGAAGTTCTC
AAGCTGGTTGCCGGCGGATCCTCCAACCGCGACATTGGCCGTATCCTCTTCCTCTCAGAAGCCACGGTGAAATCCCACCT
CGTGCACATCTACGACAAACTCGGCGTGCGGTCACGTACCTCCGCTGTCGCAGCCGCACGTGAGCAAGGGCTGCTGTAG

Upstream 100 bases:

>100_bases
AAGGCTAGACTAAAGTACGATTCATCTGCTCATCGATACTCTTGAAGGCGCATTTTCATTCGAAACGAAGTGCGCCATTG
GGAAGGACCTAGTTCAAACA

Downstream 100 bases:

>100_bases
CGGGAGGCTGCTGTAGCGGGGGTTGCTGCAAGGCTTTAGGTATCCGCGCCGGGGTTGGCCTACGGGAGCATCTCGGAGCT
TTAGCAGAAGCACGGGCTCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIRVLLADDHEIVRLGLRAVLESAEDIEVVGEVSTAEGAVQAAQEGGIDVILMDLRFGPGVQGTQVSTGADATAAIKRNI
DNPPKVLVVTNYDTDADILGAIEAGALGYLLKDAPPSELLAAVRSAAEGDSTLSPMVANRLMTRVRTPKTSLTPRELEVL
KLVAGGSSNRDIGRILFLSEATVKSHLVHIYDKLGVRSRTSAVAAAREQGLL

Sequences:

>Translated_212_residues
MIRVLLADDHEIVRLGLRAVLESAEDIEVVGEVSTAEGAVQAAQEGGIDVILMDLRFGPGVQGTQVSTGADATAAIKRNI
DNPPKVLVVTNYDTDADILGAIEAGALGYLLKDAPPSELLAAVRSAAEGDSTLSPMVANRLMTRVRTPKTSLTPRELEVL
KLVAGGSSNRDIGRILFLSEATVKSHLVHIYDKLGVRSRTSAVAAAREQGLL
>Mature_212_residues
MIRVLLADDHEIVRLGLRAVLESAEDIEVVGEVSTAEGAVQAAQEGGIDVILMDLRFGPGVQGTQVSTGADATAAIKRNI
DNPPKVLVVTNYDTDADILGAIEAGALGYLLKDAPPSELLAAVRSAAEGDSTLSPMVANRLMTRVRTPKTSLTPRELEVL
KLVAGGSSNRDIGRILFLSEATVKSHLVHIYDKLGVRSRTSAVAAAREQGLL

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=213, Percent_Identity=37.0892018779343, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1788222, Length=218, Percent_Identity=33.0275229357798, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1787473, Length=216, Percent_Identity=35.6481481481481, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=32.1608040201005, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786747, Length=217, Percent_Identity=26.7281105990783, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788546, Length=201, Percent_Identity=28.3582089552239, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788712, Length=199, Percent_Identity=26.1306532663317, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22346; Mature: 22346

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVLLADDHEIVRLGLRAVLESAEDIEVVGEVSTAEGAVQAAQEGGIDVILMDLRFGPG
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCC
VQGTQVSTGADATAAIKRNIDNPPKVLVVTNYDTDADILGAIEAGALGYLLKDAPPSELL
CCCCEECCCCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
AAVRSAAEGDSTLSPMVANRLMTRVRTPKTSLTPRELEVLKLVAGGSSNRDIGRILFLSE
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCCCEEEEEEEC
ATVKSHLVHIYDKLGVRSRTSAVAAAREQGLL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRVLLADDHEIVRLGLRAVLESAEDIEVVGEVSTAEGAVQAAQEGGIDVILMDLRFGPG
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCC
VQGTQVSTGADATAAIKRNIDNPPKVLVVTNYDTDADILGAIEAGALGYLLKDAPPSELL
CCCCEECCCCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
AAVRSAAEGDSTLSPMVANRLMTRVRTPKTSLTPRELEVLKLVAGGSSNRDIGRILFLSE
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEECCCCCCCCEEEEEEEC
ATVKSHLVHIYDKLGVRSRTSAVAAAREQGLL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]