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The map label for this gene is gdhA-2 [H]

Identifier: 145296927

GI number: 145296927

Start: 3140201

End: 3141325

Strand: Direct

Name: gdhA-2 [H]

Synonym: cgR_2827

Alternate gene names: 145296927

Gene position: 3140201-3141325 (Clockwise)

Preceding gene: 145296926

Following gene: 145296931

Centisome position: 94.75

GC content: 54.58

Gene sequence:

>1125_bases
ATGTTCGAGCTAATCGACGACTGGGGTCCCGAAAAGATCGTCATCGTCAGCGATCAAAAAACCGGGATGCGTGGCGTACT
TGTCATCGACAACACCGCCCGCGGCATGGGCAAGGGTGGCACGCGCATGCAGCCCACCGTTTCAGTCGCAGAAATAGCCA
GGTTGGCTCGCGTCATGACCTGGAAATGGGCTGGTGTAGACCTCTTTTATGGTGGTGCAAAAGCCGGAATCCAAGCAGAC
CCCACCTCCCCAGATAAAGAAGCAATCCTTCGGTCATTCGTCAGAAAACTCTCCAACGAAGTACCTAAAGAATATGTCTT
CGGCCTGGACATGGGGCTGACTGAAAATGACGCCGCCATCATCGTCGACGAGCTCGGCTGGGGCACCAGTATGGGAACAC
CCTACGAGCTCGGTGGAGTGCCCTATGACAAGCTTGGTATCACCGGCTTTGGTGTTGCAGAAGTGGTGGATCAAGTAGCA
CAAATGCAAAAACTCAAAGGTGCATCGGTAGCAGTCCAAGGCTTCGGTGCCGTTGGACATGCCACAGCTTCCCGCCTGGC
AGAACTTGGCTATCCTGTTGTGGCTATCTCCACAGCAAAGGGAGCAATCGCAGACCCCAACGGGCTCAACATCCCCGAGC
TCATGGAACTACGCGATCAGGTGGGTGACTCACTTGTGGACCACTACCCAGCACTTCGCATCAACCCAGGTGACGAACTT
TTCACCGAAGCCGAAATCCTCGTACCGGCAGCGCTCCAGGACGTCATCGATGAAGACGCAGCCAATCGACTACAAGCACA
ACTCGTCGTCGAAGGAGCAAACCTTCCCACTAATGAAGCCGCACAAAAAGTCCTAAGTAACCGTGGCATCACTGTGGTTC
CAGACTTTGTCGCCAACGCCGGAGGAGTAGTCGCCGCAGCATTCGCCATGGATAACCGCATGTCTGCATTCCGTGCAGAG
ACTGCCAACATCTTCACGAGCGTTTCCGACAAACTCCGATCCAATGCCGAAACTGTCCTGAATTTCACCTCTGAATCTGA
CCTGACCAGCCACGAAGCGGCAAGGCAACTTTCACAGGAACGAGTTCTTGCAGCTATGCGCGCCAGAGGTATGGTTCGCC
GATAA

Upstream 100 bases:

>100_bases
CGCCACCAACACCGTCAACTACTCCGGCGAACTGCCACTCGCCCAAGGCGTTAAATTCCTCTAACCCCCTCTTTAAGGAC
AGAAAGGTTTCAACCAAGAC

Downstream 100 bases:

>100_bases
CACACACCGGCACATTCCAGGTTGCAGTTTGTGGCGCAATCCGCTCTGCAGCTGCGGAGATCTAAAGACTCTCACTACTG
ATGAAAGGTGCCCATCCATT

Product: hypothetical protein

Products: NA

Alternate protein names: GDH-2 [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MFELIDDWGPEKIVIVSDQKTGMRGVLVIDNTARGMGKGGTRMQPTVSVAEIARLARVMTWKWAGVDLFYGGAKAGIQAD
PTSPDKEAILRSFVRKLSNEVPKEYVFGLDMGLTENDAAIIVDELGWGTSMGTPYELGGVPYDKLGITGFGVAEVVDQVA
QMQKLKGASVAVQGFGAVGHATASRLAELGYPVVAISTAKGAIADPNGLNIPELMELRDQVGDSLVDHYPALRINPGDEL
FTEAEILVPAALQDVIDEDAANRLQAQLVVEGANLPTNEAAQKVLSNRGITVVPDFVANAGGVVAAAFAMDNRMSAFRAE
TANIFTSVSDKLRSNAETVLNFTSESDLTSHEAARQLSQERVLAAMRARGMVRR

Sequences:

>Translated_374_residues
MFELIDDWGPEKIVIVSDQKTGMRGVLVIDNTARGMGKGGTRMQPTVSVAEIARLARVMTWKWAGVDLFYGGAKAGIQAD
PTSPDKEAILRSFVRKLSNEVPKEYVFGLDMGLTENDAAIIVDELGWGTSMGTPYELGGVPYDKLGITGFGVAEVVDQVA
QMQKLKGASVAVQGFGAVGHATASRLAELGYPVVAISTAKGAIADPNGLNIPELMELRDQVGDSLVDHYPALRINPGDEL
FTEAEILVPAALQDVIDEDAANRLQAQLVVEGANLPTNEAAQKVLSNRGITVVPDFVANAGGVVAAAFAMDNRMSAFRAE
TANIFTSVSDKLRSNAETVLNFTSESDLTSHEAARQLSQERVLAAMRARGMVRR
>Mature_374_residues
MFELIDDWGPEKIVIVSDQKTGMRGVLVIDNTARGMGKGGTRMQPTVSVAEIARLARVMTWKWAGVDLFYGGAKAGIQAD
PTSPDKEAILRSFVRKLSNEVPKEYVFGLDMGLTENDAAIIVDELGWGTSMGTPYELGGVPYDKLGITGFGVAEVVDQVA
QMQKLKGASVAVQGFGAVGHATASRLAELGYPVVAISTAKGAIADPNGLNIPELMELRDQVGDSLVDHYPALRINPGDEL
FTEAEILVPAALQDVIDEDAANRLQAQLVVEGANLPTNEAAQKVLSNRGITVVPDFVANAGGVVAAAFAMDNRMSAFRAE
TANIFTSVSDKLRSNAETVLNFTSESDLTSHEAARQLSQERVLAAMRARGMVRR

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=301, Percent_Identity=31.5614617940199, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI4885281, Length=297, Percent_Identity=31.6498316498317, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1788059, Length=304, Percent_Identity=25.6578947368421, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17544676, Length=302, Percent_Identity=30.794701986755, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319256, Length=332, Percent_Identity=24.0963855421687, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45549226, Length=305, Percent_Identity=31.1475409836066, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI45553475, Length=305, Percent_Identity=31.1475409836066, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24649283, Length=298, Percent_Identity=30.5369127516779, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24649460, Length=277, Percent_Identity=31.0469314079422, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 39920; Mature: 39920

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFELIDDWGPEKIVIVSDQKTGMRGVLVIDNTARGMGKGGTRMQPTVSVAEIARLARVMT
CCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
WKWAGVDLFYGGAKAGIQADPTSPDKEAILRSFVRKLSNEVPKEYVFGLDMGLTENDAAI
HEECCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEE
IVDELGWGTSMGTPYELGGVPYDKLGITGFGVAEVVDQVAQMQKLKGASVAVQGFGAVGH
EEECCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHH
ATASRLAELGYPVVAISTAKGAIADPNGLNIPELMELRDQVGDSLVDHYPALRINPGDEL
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHH
FTEAEILVPAALQDVIDEDAANRLQAQLVVEGANLPTNEAAQKVLSNRGITVVPDFVANA
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCEEECHHHCCC
GGVVAAAFAMDNRMSAFRAETANIFTSVSDKLRSNAETVLNFTSESDLTSHEAARQLSQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCCHHHHHHHHHHHH
RVLAAMRARGMVRR
HHHHHHHHCCCCCC
>Mature Secondary Structure
MFELIDDWGPEKIVIVSDQKTGMRGVLVIDNTARGMGKGGTRMQPTVSVAEIARLARVMT
CCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
WKWAGVDLFYGGAKAGIQADPTSPDKEAILRSFVRKLSNEVPKEYVFGLDMGLTENDAAI
HEECCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEE
IVDELGWGTSMGTPYELGGVPYDKLGITGFGVAEVVDQVAQMQKLKGASVAVQGFGAVGH
EEECCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHH
ATASRLAELGYPVVAISTAKGAIADPNGLNIPELMELRDQVGDSLVDHYPALRINPGDEL
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHH
FTEAEILVPAALQDVIDEDAANRLQAQLVVEGANLPTNEAAQKVLSNRGITVVPDFVANA
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCEEECHHHCCC
GGVVAAAFAMDNRMSAFRAETANIFTSVSDKLRSNAETVLNFTSESDLTSHEAARQLSQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCCHHHHHHHHHHHH
RVLAAMRARGMVRR
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726; 1730244 [H]