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The map label for this gene is grpE

Identifier: 145296786

GI number: 145296786

Start: 2976385

End: 2977041

Strand: Reverse

Name: grpE

Synonym: cgR_2689

Alternate gene names: 145296786

Gene position: 2977041-2976385 (Counterclockwise)

Preceding gene: 145296787

Following gene: 145296785

Centisome position: 89.83

GC content: 54.95

Gene sequence:

>657_bases
ATGACTACCCCTAACGGAATGCCCGACAATCCTGGGGATCCTGAAAACACCGATCCAGAGGCAACCTCTGCTGATCGTGC
TGAGCAAGCAGCTGAAGAAGCAGCTGCCCGTCAAGCGGAGGAATCTCCATCTGGACAGGCCTTAGAGGAAGAAATTTCTC
CAGAGCTCGAAGCGGAGATCAATGATCTTCTATCAGATGTTGATCCAGATTTGGATGGCGATGGTGAAGTGTCCGCTGTA
GAAACACAGCTTGCCGAACGCACTGAAGATCTCCAGCGAGTCACCGCAGAGTACGCCAACTACCGTCGACGTACCGAGCG
TGAACGCCAGGGCATCATCGACACCGCACGTGCCAGCGTTGTTACTCAGCTGCTGCCGTTGCTCGACGATCTTGACCTGG
CTGAACAGCACGGTGACCTTAACGAAGGTCCGCTGAAGTCACTGTCTGACAAGCTGATCAACATCCTGGGTGGATTGAAA
GTGGAATCCTTCGGCGAGATCGGCGAAGCATTCGATCCAGAGATCCACGAAGCAGTACAGGATCTCTCACAGGGTGATGT
CAAGGTTCTGGGAACGGTACTCCGCAAGGGATACCGTCTCGGCGACCGCGTCATCCGCACCGCAATGGTCCTCATTGGGG
ATCCAGAGGAGAGCTAG

Upstream 100 bases:

>100_bases
CCAGGCTGACGCAGGTGCAGAAGGCGCTGCAGATGACAATGTTGTTGACGCTGAAGTTGTCGAAGACGACGCAGCTGACA
ATGGTGAGGATAAGAAGTAA

Downstream 100 bases:

>100_bases
AGAGACTAAGTCTCTTAGTGGTTTCCGCCGTTGTCCACGGTCCACAGATAGAACTCCTTGAACAACACCGGCGGATATTC
TTGAAGTAATAAATACTCAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTTPNGMPDNPGDPENTDPEATSADRAEQAAEEAAARQAEESPSGQALEEEISPELEAEINDLLSDVDPDLDGDGEVSAV
ETQLAERTEDLQRVTAEYANYRRRTERERQGIIDTARASVVTQLLPLLDDLDLAEQHGDLNEGPLKSLSDKLINILGGLK
VESFGEIGEAFDPEIHEAVQDLSQGDVKVLGTVLRKGYRLGDRVIRTAMVLIGDPEES

Sequences:

>Translated_218_residues
MTTPNGMPDNPGDPENTDPEATSADRAEQAAEEAAARQAEESPSGQALEEEISPELEAEINDLLSDVDPDLDGDGEVSAV
ETQLAERTEDLQRVTAEYANYRRRTERERQGIIDTARASVVTQLLPLLDDLDLAEQHGDLNEGPLKSLSDKLINILGGLK
VESFGEIGEAFDPEIHEAVQDLSQGDVKVLGTVLRKGYRLGDRVIRTAMVLIGDPEES
>Mature_217_residues
TTPNGMPDNPGDPENTDPEATSADRAEQAAEEAAARQAEESPSGQALEEEISPELEAEINDLLSDVDPDLDGDGEVSAVE
TQLAERTEDLQRVTAEYANYRRRTERERQGIIDTARASVVTQLLPLLDDLDLAEQHGDLNEGPLKSLSDKLINILGGLKV
ESFGEIGEAFDPEIHEAVQDLSQGDVKVLGTVLRKGYRLGDRVIRTAMVLIGDPEES

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=198, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_CORGB (A4QHI9)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139607.1
- ProteinModelPortal:   A4QHI9
- SMR:   A4QHI9
- STRING:   A4QHI9
- GeneID:   4993203
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2689
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   FKTANQE
- ProtClustDB:   PRK14164
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 23676; Mature: 23545

Theoretical pI: Translated: 3.87; Mature: 3.87

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPNGMPDNPGDPENTDPEATSADRAEQAAEEAAARQAEESPSGQALEEEISPELEAEI
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHH
NDLLSDVDPDLDGDGEVSAVETQLAERTEDLQRVTAEYANYRRRTERERQGIIDTARASV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTQLLPLLDDLDLAEQHGDLNEGPLKSLSDKLINILGGLKVESFGEIGEAFDPEIHEAVQ
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
DLSQGDVKVLGTVLRKGYRLGDRVIRTAMVLIGDPEES
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTPNGMPDNPGDPENTDPEATSADRAEQAAEEAAARQAEESPSGQALEEEISPELEAEI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHH
NDLLSDVDPDLDGDGEVSAVETQLAERTEDLQRVTAEYANYRRRTERERQGIIDTARASV
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTQLLPLLDDLDLAEQHGDLNEGPLKSLSDKLINILGGLKVESFGEIGEAFDPEIHEAVQ
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
DLSQGDVKVLGTVLRKGYRLGDRVIRTAMVLIGDPEES
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA