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The map label for this gene is pyrE

Identifier: 145296766

GI number: 145296766

Start: 2955149

End: 2955703

Strand: Reverse

Name: pyrE

Synonym: cgR_2670

Alternate gene names: 145296766

Gene position: 2955703-2955149 (Counterclockwise)

Preceding gene: 145296767

Following gene: 145296765

Centisome position: 89.18

GC content: 58.38

Gene sequence:

>555_bases
ATGTCATCTAATTCCATTAACGTAGAAGCGCGCGCTGAGCTTGCTGAACTGATCAAAGAGCTAGCTGTCGTCCACGGTGA
AGTCACCTTGTCTTCGGGCAAGAAGGCTGATTACTACATCGATGTCCGTCGTGCTACCTTGCACGCGCGCGCATCTCGCC
TGATCGGTCAGCTGCTGCGTGAAGCCACCGCTGACTGGGACTATGACGCAGTTGGCGGCCTGACCTTGGGTGCTGACCCG
GTTGCCACCGCCATCATGCACGCCGACGGCCGCGATATCAACGCGTTTGTGGTGCGCAAGGAAGCCAAGAAGCACGGCAT
GCAGCGTCGCATTGAGGGCCCTGACCTGACGGGCAAGAAGGTGCTCGTGGTGGAAGATACCACCACTACCGGAAATTCCC
CTCTGACAGCTGTCGCCGCGTTGCGTGAAGCTGGCATTGAGGTTGTGGGCGTTGCCACCGTGGTCGATCGCGCAACCGGC
GCTGATGAGGTTATCGCAGCGGAAGGTCTTCCTTACCGCAGCTTGCTGGGACTTTCTGACCTTGGACTCAACTAA

Upstream 100 bases:

>100_bases
GAAAACTGGTGTTTTTCGGCCGTGTCCACCCCAGGTTCTATGCTGTAACAAACGCGGGTTTAAACCTCAATCATCAAATT
AGGGAAGGGCTGGGAAATCC

Downstream 100 bases:

>100_bases
CACCCCCGGCCCCACGGAGTGGGGCGAGTCCCGCGTGGGCAAAGGTCCATGGGAAGAGGAAAACCCCGGCGTACCTCGGC
CTACAAACCCGCTTTTCGAC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MSSNSINVEARAELAELIKELAVVHGEVTLSSGKKADYYIDVRRATLHARASRLIGQLLREATADWDYDAVGGLTLGADP
VATAIMHADGRDINAFVVRKEAKKHGMQRRIEGPDLTGKKVLVVEDTTTTGNSPLTAVAALREAGIEVVGVATVVDRATG
ADEVIAAEGLPYRSLLGLSDLGLN

Sequences:

>Translated_184_residues
MSSNSINVEARAELAELIKELAVVHGEVTLSSGKKADYYIDVRRATLHARASRLIGQLLREATADWDYDAVGGLTLGADP
VATAIMHADGRDINAFVVRKEAKKHGMQRRIEGPDLTGKKVLVVEDTTTTGNSPLTAVAALREAGIEVVGVATVVDRATG
ADEVIAAEGLPYRSLLGLSDLGLN
>Mature_183_residues
SSNSINVEARAELAELIKELAVVHGEVTLSSGKKADYYIDVRRATLHARASRLIGQLLREATADWDYDAVGGLTLGADPV
ATAIMHADGRDINAFVVRKEAKKHGMQRRIEGPDLTGKKVLVVEDTTTTGNSPLTAVAALREAGIEVVGVATVVDRATGA
DEVIAAEGLPYRSLLGLSDLGLN

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=178, Percent_Identity=30.8988764044944, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508631, Length=158, Percent_Identity=32.9113924050633, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17554964, Length=173, Percent_Identity=28.9017341040462, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_CORGB (A4QHG9)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139587.1
- ProteinModelPortal:   A4QHG9
- SMR:   A4QHG9
- STRING:   A4QHG9
- GeneID:   4993852
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2670
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   GKEADYY
- ProtClustDB:   PRK00455
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 19473; Mature: 19342

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER; PS00589 PTS_HPR_SER

Important sites: BINDING 99-99 BINDING 100-100 BINDING 103-103 BINDING 105-105 BINDING 129-129 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNSINVEARAELAELIKELAVVHGEVTLSSGKKADYYIDVRRATLHARASRLIGQLLR
CCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
EATADWDYDAVGGLTLGADPVATAIMHADGRDINAFVVRKEAKKHGMQRRIEGPDLTGKK
HHCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCE
VLVVEDTTTTGNSPLTAVAALREAGIEVVGVATVVDRATGADEVIAAEGLPYRSLLGLSD
EEEEECCCCCCCCCHHHHHHHHHCCCEEEEHHHHHHCCCCCCCEEECCCCCHHHHHCCCC
LGLN
CCCC
>Mature Secondary Structure 
SSNSINVEARAELAELIKELAVVHGEVTLSSGKKADYYIDVRRATLHARASRLIGQLLR
CCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
EATADWDYDAVGGLTLGADPVATAIMHADGRDINAFVVRKEAKKHGMQRRIEGPDLTGKK
HHCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCE
VLVVEDTTTTGNSPLTAVAALREAGIEVVGVATVVDRATGADEVIAAEGLPYRSLLGLSD
EEEEECCCCCCCCCHHHHHHHHHCCCEEEEHHHHHHCCCCCCCEEECCCCCHHHHHCCCC
LGLN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA