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The map label for this gene is cls [H]

Identifier: 145296741

GI number: 145296741

Start: 2925837

End: 2927339

Strand: Direct

Name: cls [H]

Synonym: cgR_2645

Alternate gene names: 145296741

Gene position: 2925837-2927339 (Clockwise)

Preceding gene: 145296740

Following gene: 145296743

Centisome position: 88.28

GC content: 56.95

Gene sequence:

>1503_bases
ATGATCTTTCAGATCAACCTCGAATCTTGGCAAACCGTTGGTTTGATCATCGACTACACCATCAAAATCATCGCCATTGG
CTACGTACCCGAAGGACGCAGACCCAGCTCCTCCACCGCGTGGCTCCTGGCAATTTTGCTGCTCCCCTACGTCGGACTCC
CACTGTTCCTGCTTATGGGATCGCCATACATCAACCGGCGACGCCACCGCATCCAACAAGAAATCAACGACCTCATCGAA
GACGTCCACGACGACGTCCCCGACATCCCCACCGGAATGGATGTCTCCGCGGAAGTTGAATCTGTCATCAAACTCAACCG
CCGCCTCACCCGCATGCCAGCAGTGACCGGCGGAAACAACGGCTTCTACTCCGACTACCGTGAATCCCTCAAACGGATGA
CCGCCGCAATCGACGAAGCCGAAGAATACATCTACGTCGAGATCTACATCATGGCCTGGGATTCCTACACCCAACCATTC
TTCGCAGCACTCGAACGAGCCCATAACCGCGGCGTCAAAGTCCGACTCCTTTTCGACCACGTCGGCAGCTGGAAATACCC
CGGCTACCACCGTCTCAAAAAAGAACTCAACCGCATGGGCTTCGCCTGGTACCTCATGCTCCCCCTCCAACCCTGGCGAC
GCCGCTTCCGCCGACCCGACCTGCGCAACCACCGCAAAATGCTCATCATCGACGGCCACACCGCCTTCATGGGCTCCCAA
AATCTCATCGCCCCGAGTTACCTACAAAAGAAAAACATCAAACTCGGCCGCGAATGGAAAGACCTCATGGTCGAACTCAC
CGGCCCCATCGTCTCCTCCATGGAAATGATCTTCGCCGGCGACTGGTACGTCGAATCCAACGAAGCCCTCGACATCCGCG
ACCACGCAGAAGCCCACGGCTACATCGGCAACACTCAAAAAGACTCCGCCACCAACCTCGTGCAGCTCATCCCCTCCGGC
CCTGGTTACACCACAGAACCCAACCTGCGCATGTTCAACTCCATCGTTCACCACGCCAAAGAACGACTCATCTTGTGCAG
CCCCTACTTCATCCCCGACGAATCCCTCCTCGAAGCCGTCACCTCAGCCTGCTACCGCGGAGTAACCGTCGAACTATTCG
TCTCTGAACAAGCCGACCAATTCGCCATCGACCACGCCCAATCCTCCTACTACCAGGCACTCCTTGAAGCCGGCGTGAAA
ATCTACCAATTCCCCAAACCCGACGTCCTCCACACCAAGTACATGATCGCCGACCCCGACGACACCACCGGCAACGAAGC
CCTCGGAGTCCTCGGATCCTCCAACCTCGACATCCGCAGCTTTGGCCTCAACTACGAAATCTCCCTGATGATCGCCAAAG
GCAACCTCATCCACGAACTCAACGCCCTCACGGACCGTTACCGCACAGTAAGTCTCAAGCTCACCTTGGATAAGTGGAAC
CAGCGCAGTTGGCGGCGCCGCTACGTGGACAATGTCATGCGTTTGACCTCGGCGCTGCAGTAG

Upstream 100 bases:

>100_bases
TGCAACCGCGGGTGAAACCTTTGTGGACGTTGAAGAACGCAGCGGAACCGGCGCCTCTGACCACGCACCAGTCATCGTTG
ATTACAAGGTGTAACTGCGT

Downstream 100 bases:

>100_bases
TTTGGCGCGTTTGGAGTGCGTTTGAGGTGGCTTTTAGAGGGACCGGCTACACCCCACCGCCATTATGCAAATATTCGTTC
GAAACTTTGGTCGGGCCACG

Product: hypothetical protein

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MIFQINLESWQTVGLIIDYTIKIIAIGYVPEGRRPSSSTAWLLAILLLPYVGLPLFLLMGSPYINRRRHRIQQEINDLIE
DVHDDVPDIPTGMDVSAEVESVIKLNRRLTRMPAVTGGNNGFYSDYRESLKRMTAAIDEAEEYIYVEIYIMAWDSYTQPF
FAALERAHNRGVKVRLLFDHVGSWKYPGYHRLKKELNRMGFAWYLMLPLQPWRRRFRRPDLRNHRKMLIIDGHTAFMGSQ
NLIAPSYLQKKNIKLGREWKDLMVELTGPIVSSMEMIFAGDWYVESNEALDIRDHAEAHGYIGNTQKDSATNLVQLIPSG
PGYTTEPNLRMFNSIVHHAKERLILCSPYFIPDESLLEAVTSACYRGVTVELFVSEQADQFAIDHAQSSYYQALLEAGVK
IYQFPKPDVLHTKYMIADPDDTTGNEALGVLGSSNLDIRSFGLNYEISLMIAKGNLIHELNALTDRYRTVSLKLTLDKWN
QRSWRRRYVDNVMRLTSALQ

Sequences:

>Translated_500_residues
MIFQINLESWQTVGLIIDYTIKIIAIGYVPEGRRPSSSTAWLLAILLLPYVGLPLFLLMGSPYINRRRHRIQQEINDLIE
DVHDDVPDIPTGMDVSAEVESVIKLNRRLTRMPAVTGGNNGFYSDYRESLKRMTAAIDEAEEYIYVEIYIMAWDSYTQPF
FAALERAHNRGVKVRLLFDHVGSWKYPGYHRLKKELNRMGFAWYLMLPLQPWRRRFRRPDLRNHRKMLIIDGHTAFMGSQ
NLIAPSYLQKKNIKLGREWKDLMVELTGPIVSSMEMIFAGDWYVESNEALDIRDHAEAHGYIGNTQKDSATNLVQLIPSG
PGYTTEPNLRMFNSIVHHAKERLILCSPYFIPDESLLEAVTSACYRGVTVELFVSEQADQFAIDHAQSSYYQALLEAGVK
IYQFPKPDVLHTKYMIADPDDTTGNEALGVLGSSNLDIRSFGLNYEISLMIAKGNLIHELNALTDRYRTVSLKLTLDKWN
QRSWRRRYVDNVMRLTSALQ
>Mature_500_residues
MIFQINLESWQTVGLIIDYTIKIIAIGYVPEGRRPSSSTAWLLAILLLPYVGLPLFLLMGSPYINRRRHRIQQEINDLIE
DVHDDVPDIPTGMDVSAEVESVIKLNRRLTRMPAVTGGNNGFYSDYRESLKRMTAAIDEAEEYIYVEIYIMAWDSYTQPF
FAALERAHNRGVKVRLLFDHVGSWKYPGYHRLKKELNRMGFAWYLMLPLQPWRRRFRRPDLRNHRKMLIIDGHTAFMGSQ
NLIAPSYLQKKNIKLGREWKDLMVELTGPIVSSMEMIFAGDWYVESNEALDIRDHAEAHGYIGNTQKDSATNLVQLIPSG
PGYTTEPNLRMFNSIVHHAKERLILCSPYFIPDESLLEAVTSACYRGVTVELFVSEQADQFAIDHAQSSYYQALLEAGVK
IYQFPKPDVLHTKYMIADPDDTTGNEALGVLGSSNLDIRSFGLNYEISLMIAKGNLIHELNALTDRYRTVSLKLTLDKWN
QRSWRRRYVDNVMRLTSALQ

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=498, Percent_Identity=29.1164658634538, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1787007, Length=335, Percent_Identity=26.2686567164179, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 57551; Mature: 57551

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFQINLESWQTVGLIIDYTIKIIAIGYVPEGRRPSSSTAWLLAILLLPYVGLPLFLLMG
CEEEEECCCCCEEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
SPYINRRRHRIQQEINDLIEDVHDDVPDIPTGMDVSAEVESVIKLNRRLTRMPAVTGGNN
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCC
GFYSDYRESLKRMTAAIDEAEEYIYVEIYIMAWDSYTQPFFAALERAHNRGVKVRLLFDH
CCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEC
VGSWKYPGYHRLKKELNRMGFAWYLMLPLQPWRRRFRRPDLRNHRKMLIIDGHTAFMGSQ
CCCCCCCCHHHHHHHHHHCCEEEEEEECCCHHHHHCCCCCCCCCCEEEEECCCEEEECCC
NLIAPSYLQKKNIKLGREWKDLMVELTGPIVSSMEMIFAGDWYVESNEALDIRDHAEAHG
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCEEEECCCEECCHHHHHHCC
YIGNTQKDSATNLVQLIPSGPGYTTEPNLRMFNSIVHHAKERLILCSPYFIPDESLLEAV
CCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHH
TSACYRGVTVELFVSEQADQFAIDHAQSSYYQALLEAGVKIYQFPKPDVLHTKYMIADPD
HHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCC
DTTGNEALGVLGSSNLDIRSFGLNYEISLMIAKGNLIHELNALTDRYRTVSLKLTLDKWN
CCCCCCEEEEECCCCCEEEEECCCEEEEEEEECCCHHHHHHHHHHCEEEEEEEEEECCCC
QRSWRRRYVDNVMRLTSALQ
CHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIFQINLESWQTVGLIIDYTIKIIAIGYVPEGRRPSSSTAWLLAILLLPYVGLPLFLLMG
CEEEEECCCCCEEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
SPYINRRRHRIQQEINDLIEDVHDDVPDIPTGMDVSAEVESVIKLNRRLTRMPAVTGGNN
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCC
GFYSDYRESLKRMTAAIDEAEEYIYVEIYIMAWDSYTQPFFAALERAHNRGVKVRLLFDH
CCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEC
VGSWKYPGYHRLKKELNRMGFAWYLMLPLQPWRRRFRRPDLRNHRKMLIIDGHTAFMGSQ
CCCCCCCCHHHHHHHHHHCCEEEEEEECCCHHHHHCCCCCCCCCCEEEEECCCEEEECCC
NLIAPSYLQKKNIKLGREWKDLMVELTGPIVSSMEMIFAGDWYVESNEALDIRDHAEAHG
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCEEEECCCEECCHHHHHHCC
YIGNTQKDSATNLVQLIPSGPGYTTEPNLRMFNSIVHHAKERLILCSPYFIPDESLLEAV
CCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHH
TSACYRGVTVELFVSEQADQFAIDHAQSSYYQALLEAGVKIYQFPKPDVLHTKYMIADPD
HHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCC
DTTGNEALGVLGSSNLDIRSFGLNYEISLMIAKGNLIHELNALTDRYRTVSLKLTLDKWN
CCCCCCEEEEECCCCCEEEEECCCEEEEEEEECCCHHHHHHHHHHCEEEEEEEEEECCCC
QRSWRRRYVDNVMRLTSALQ
CHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA