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The map label for this gene is folE

Identifier: 145296691

GI number: 145296691

Start: 2866636

End: 2867226

Strand: Reverse

Name: folE

Synonym: cgR_2596

Alternate gene names: 145296691

Gene position: 2867226-2866636 (Counterclockwise)

Preceding gene: 145296692

Following gene: 145296690

Centisome position: 86.51

GC content: 58.21

Gene sequence:

>591_bases
GTGGATAACCACGCTGCAGTTCGCGAGTTCGATGAGGAGCGCGCAACAGCTGCGATTCGTGAGTTGCTCATCGCTGTGGG
TGAGGATCCAGATCGCGAAGGCCTGTTGGAAACCCCAGCTCGAGTGGCTAGGGCGTACAAGGAAACCTTCGCGGGTCTGC
ATGAGGATCCCACCACTGTGCTGGAGAAGACGTTCTCTGAGGGCCATGAAGAGTTGGTTCTGGTTCGTGAGATCCCGATT
TACTCCATGTGTGAGCACCACTTGGTGCCGTTCTTTGGCGTGGCGCACATTGGTTACATTCCGGGTAAGTCCGGCAAGGT
GACTGGCCTGTCCAAGCTGGCGCGTTTAGCGGATATGCTTGCTAAGCGACCTCAGGTTCAGGAGCGCTTGACCTCCCAAA
TTGCGGATGCTCTCGTCGAGAAGCTTGATGCCCAGGCCGTGGCCGTGGTGATTGAAGCTGAGCACCTGTGCATGGCCATG
CGCGGAATCCGTAAGCCTGGCGCTGTGACCACGACGTCTGCGGTGCGCGGCGGCTTTAAGAACAATGCTGCCTCCCGTGC
TGAGGTGTTCTCCCTGATTCGGGGGCACTAA

Upstream 100 bases:

>100_bases
ACCACAGAAATGCCTGTCGTTCCAGATCAGCCCATCGATGGTGATTCCGGGAAGTCCGCTGAGGGCACACAGGAGAATCC
GGAAAATGAAGGAGACAACC

Downstream 100 bases:

>100_bases
ATGAACGTATCCTCTTTGACCATCCCGGGACGCTGTTTGGTCATGGGAATTGTCAATGTCACTGAGGATTCCTTTTCGGA
CGGTGGCAAGTACATTGACG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MDNHAAVREFDEERATAAIRELLIAVGEDPDREGLLETPARVARAYKETFAGLHEDPTTVLEKTFSEGHEELVLVREIPI
YSMCEHHLVPFFGVAHIGYIPGKSGKVTGLSKLARLADMLAKRPQVQERLTSQIADALVEKLDAQAVAVVIEAEHLCMAM
RGIRKPGAVTTTSAVRGGFKNNAASRAEVFSLIRGH

Sequences:

>Translated_196_residues
MDNHAAVREFDEERATAAIRELLIAVGEDPDREGLLETPARVARAYKETFAGLHEDPTTVLEKTFSEGHEELVLVREIPI
YSMCEHHLVPFFGVAHIGYIPGKSGKVTGLSKLARLADMLAKRPQVQERLTSQIADALVEKLDAQAVAVVIEAEHLCMAM
RGIRKPGAVTTTSAVRGGFKNNAASRAEVFSLIRGH
>Mature_196_residues
MDNHAAVREFDEERATAAIRELLIAVGEDPDREGLLETPARVARAYKETFAGLHEDPTTVLEKTFSEGHEELVLVREIPI
YSMCEHHLVPFFGVAHIGYIPGKSGKVTGLSKLARLADMLAKRPQVQERLTSQIADALVEKLDAQAVAVVIEAEHLCMAM
RGIRKPGAVTTTSAVRGGFKNNAASRAEVFSLIRGH

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=187, Percent_Identity=47.0588235294118, Blast_Score=171, Evalue=4e-43,
Organism=Homo sapiens, GI4503949, Length=187, Percent_Identity=47.0588235294118, Blast_Score=171, Evalue=4e-43,
Organism=Homo sapiens, GI66932970, Length=156, Percent_Identity=46.1538461538462, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI66932972, Length=146, Percent_Identity=48.6301369863014, Blast_Score=133, Evalue=9e-32,
Organism=Escherichia coli, GI1788476, Length=198, Percent_Identity=40.4040404040404, Blast_Score=117, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=50.2824858757062, Blast_Score=179, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6321706, Length=183, Percent_Identity=52.4590163934426, Blast_Score=181, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24656782, Length=178, Percent_Identity=44.9438202247191, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24656796, Length=196, Percent_Identity=42.3469387755102, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24656791, Length=196, Percent_Identity=42.3469387755102, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_CORGB (A4QH94)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139512.1
- ProteinModelPortal:   A4QH94
- SMR:   A4QH94
- STRING:   A4QH94
- GeneID:   4993831
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2596
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   MAPIIGR
- ProtClustDB:   PRK09347
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 21438; Mature: 21438

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNHAAVREFDEERATAAIRELLIAVGEDPDREGLLETPARVARAYKETFAGLHEDPTTV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCHHHH
LEKTFSEGHEELVLVREIPIYSMCEHHLVPFFGVAHIGYIPGKSGKVTGLSKLARLADML
HHHHHHCCHHHEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AKRPQVQERLTSQIADALVEKLDAQAVAVVIEAEHLCMAMRGIRKPGAVTTTSAVRGGFK
HCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
NNAASRAEVFSLIRGH
CCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDNHAAVREFDEERATAAIRELLIAVGEDPDREGLLETPARVARAYKETFAGLHEDPTTV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCHHHH
LEKTFSEGHEELVLVREIPIYSMCEHHLVPFFGVAHIGYIPGKSGKVTGLSKLARLADML
HHHHHHCCHHHEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AKRPQVQERLTSQIADALVEKLDAQAVAVVIEAEHLCMAMRGIRKPGAVTTTSAVRGGFK
HCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
NNAASRAEVFSLIRGH
CCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA