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The map label for this gene is ispD

Identifier: 145296657

GI number: 145296657

Start: 2830818

End: 2831588

Strand: Reverse

Name: ispD

Synonym: cgR_2564

Alternate gene names: 145296657

Gene position: 2831588-2830818 (Counterclockwise)

Preceding gene: 145296658

Following gene: 145296656

Centisome position: 85.44

GC content: 59.4

Gene sequence:

>771_bases
ATGTCGTCTACACGAATCCCCGTCATCGCACTCCTCGCGGCGGCGGGGCGCGGAACCCGCCTCGGCGGACCCATCCCCAA
AGCATTCGTCACGCTGCGTGAACGCACACTTTTAGAGCGCTCGCTCCAAGCCATGCTCACCTCCGAAAGCGTCGACGAAA
TCATCATCCTCGTCAGCCCCGACATGGAAACCTACGCCCGCGATTTGCTGCGCAAACGCGGTCTTTTGAACGACCCCGAA
GGGGTACGCATACGGCTCGTGCACGGCGGCGGGGAGCGCGCGGACTCGGTCTGGGCAGGCCTTCAGGCAATTTTGCTTGA
CGACGCCACCCCCGATGCAATTGTCTTAATCCACGACAGCGCCCGAGCGCTCACACCACCCGGCATGATTGCGCGCGTGG
TGCGCAAAGTCCACGAAGGCGCCACCGCAGTCATCCCAGTACTGCCAGTATCGGACACCATCAAACGCGTTTCCCTTGAT
GCCGGAGTAGTTGTCGACACTCCCAACCGTGCAGAGCTTCGCGCCGTCCAAACCCCGCAAGGCTTCCTGCTGTCCGAACT
TGTTGCAGCGAATAAGAAATTCTTCGCCGACCCCAACCCAGGCTTCATCCCAACCGATGACGCCAGCCTGATGGAATGGT
ACGGCGCAGATGTAGTCTGCGTACAAGGCGACCCAATGGCGTTTAAAGTAACAACCCCCATCGACATGATGCTGGCGCAA
CGCATCACTGATGAAGCCGAACCCACAATATTTGAGGTACCAGGTGACTAA

Upstream 100 bases:

>100_bases
GCTCGGCGACGAGGAAGAGAAGAAGGACGCATTCGACGACTTCGACGATTCCGACGTGGATCTTGACGATCTGAGCTTCG
ACGACGAAGATTAGACGCCC

Downstream 100 bases:

>100_bases
CCCAATCATCCCCCGCGTAGGAATCGCCACAGACGCCCACCAAATCGAAGCCGGAAAACCCTGTTGGATCGCCTGCCTCC
TCTTTGAAGGCGTCGATGGC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSSTRIPVIALLAAAGRGTRLGGPIPKAFVTLRERTLLERSLQAMLTSESVDEIIILVSPDMETYARDLLRKRGLLNDPE
GVRIRLVHGGGERADSVWAGLQAILLDDATPDAIVLIHDSARALTPPGMIARVVRKVHEGATAVIPVLPVSDTIKRVSLD
AGVVVDTPNRAELRAVQTPQGFLLSELVAANKKFFADPNPGFIPTDDASLMEWYGADVVCVQGDPMAFKVTTPIDMMLAQ
RITDEAEPTIFEVPGD

Sequences:

>Translated_256_residues
MSSTRIPVIALLAAAGRGTRLGGPIPKAFVTLRERTLLERSLQAMLTSESVDEIIILVSPDMETYARDLLRKRGLLNDPE
GVRIRLVHGGGERADSVWAGLQAILLDDATPDAIVLIHDSARALTPPGMIARVVRKVHEGATAVIPVLPVSDTIKRVSLD
AGVVVDTPNRAELRAVQTPQGFLLSELVAANKKFFADPNPGFIPTDDASLMEWYGADVVCVQGDPMAFKVTTPIDMMLAQ
RITDEAEPTIFEVPGD
>Mature_255_residues
SSTRIPVIALLAAAGRGTRLGGPIPKAFVTLRERTLLERSLQAMLTSESVDEIIILVSPDMETYARDLLRKRGLLNDPEG
VRIRLVHGGGERADSVWAGLQAILLDDATPDAIVLIHDSARALTPPGMIARVVRKVHEGATAVIPVLPVSDTIKRVSLDA
GVVVDTPNRAELRAVQTPQGFLLSELVAANKKFFADPNPGFIPTDDASLMEWYGADVVCVQGDPMAFKVTTPIDMMLAQR
ITDEAEPTIFEVPGD

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=244, Percent_Identity=28.6885245901639, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1789104, Length=240, Percent_Identity=34.1666666666667, Blast_Score=104, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_CORGB (A4QH60)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139478.1
- ProteinModelPortal:   A4QH60
- SMR:   A4QH60
- STRING:   A4QH60
- GeneID:   4993666
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2564
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- ProtClustDB:   PRK00155
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 27594; Mature: 27463

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTRIPVIALLAAAGRGTRLGGPIPKAFVTLRERTLLERSLQAMLTSESVDEIIILVSP
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
DMETYARDLLRKRGLLNDPEGVRIRLVHGGGERADSVWAGLQAILLDDATPDAIVLIHDS
CHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHEEEECCCCCCEEEEEECC
ARALTPPGMIARVVRKVHEGATAVIPVLPVSDTIKRVSLDAGVVVDTPNRAELRAVQTPQ
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHEECCCCEEEECCCCCCEEEECCCC
GFLLSELVAANKKFFADPNPGFIPTDDASLMEWYGADVVCVQGDPMAFKVTTPIDMMLAQ
CHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHH
RITDEAEPTIFEVPGD
HHCCCCCCEEEECCCC
>Mature Secondary Structure 
SSTRIPVIALLAAAGRGTRLGGPIPKAFVTLRERTLLERSLQAMLTSESVDEIIILVSP
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
DMETYARDLLRKRGLLNDPEGVRIRLVHGGGERADSVWAGLQAILLDDATPDAIVLIHDS
CHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHEEEECCCCCCEEEEEECC
ARALTPPGMIARVVRKVHEGATAVIPVLPVSDTIKRVSLDAGVVVDTPNRAELRAVQTPQ
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHEECCCCEEEECCCCCCEEEECCCC
GFLLSELVAANKKFFADPNPGFIPTDDASLMEWYGADVVCVQGDPMAFKVTTPIDMMLAQ
CHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHH
RITDEAEPTIFEVPGD
HHCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA