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The map label for this gene is ispF

Identifier: 145296656

GI number: 145296656

Start: 2830343

End: 2830825

Strand: Reverse

Name: ispF

Synonym: cgR_2563

Alternate gene names: 145296656

Gene position: 2830825-2830343 (Counterclockwise)

Preceding gene: 145296657

Following gene: 145296655

Centisome position: 85.42

GC content: 57.56

Gene sequence:

>483_bases
GTGACTAACCCAATCATCCCCCGCGTAGGAATCGCCACAGACGCCCACCAAATCGAAGCCGGAAAACCCTGTTGGATCGC
CTGCCTCCTCTTTGAAGGCGTCGATGGCTGCGAAGGCCACTCCGACGGGGATGTGATTGCCCACGCGATCGTGGACGCAC
TCCTTTCCGCCTCTGGCCTGGGTGATCTTGGTTCCTTCGTGGGTGTTGGTCGCCCAGAATACGACGGAGTCTCAGGTACG
CAGTTGTTGAAGGAAGTCCGGGAACTTCTTGAGTCTCGTGGATTTGTGATTGGAAACGTTGCTGCCCAGCTAGTCGGCCA
AACCCCCAAATTTGGTCCCCGGCGCGAAGAAGCACAGCAAGTGATCTCTGACATCATCGGCGCACCATGCTCACTGTCTG
CCACCACCACTGATCACATGGGATTCACTGGCCGCAGCGAAGGCCGTGCTTCGGTAGCGACAGCTGTGGTGTGGAAGGTT
TAA

Upstream 100 bases:

>100_bases
CAAGGCGACCCAATGGCGTTTAAAGTAACAACCCCCATCGACATGATGCTGGCGCAACGCATCACTGATGAAGCCGAACC
CACAATATTTGAGGTACCAG

Downstream 100 bases:

>100_bases
GTTTTCTGTAGGGATTGGGCTGACTTGGTCTGTTTGGAGCTGGCGGGGGCTGGCGGGGGCTGGCTTCAATGTACGGTCGG
GGCATGCCCAAGCTGAGTTC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTNPIIPRVGIATDAHQIEAGKPCWIACLLFEGVDGCEGHSDGDVIAHAIVDALLSASGLGDLGSFVGVGRPEYDGVSGT
QLLKEVRELLESRGFVIGNVAAQLVGQTPKFGPRREEAQQVISDIIGAPCSLSATTTDHMGFTGRSEGRASVATAVVWKV

Sequences:

>Translated_160_residues
MTNPIIPRVGIATDAHQIEAGKPCWIACLLFEGVDGCEGHSDGDVIAHAIVDALLSASGLGDLGSFVGVGRPEYDGVSGT
QLLKEVRELLESRGFVIGNVAAQLVGQTPKFGPRREEAQQVISDIIGAPCSLSATTTDHMGFTGRSEGRASVATAVVWKV
>Mature_159_residues
TNPIIPRVGIATDAHQIEAGKPCWIACLLFEGVDGCEGHSDGDVIAHAIVDALLSASGLGDLGSFVGVGRPEYDGVSGTQ
LLKEVRELLESRGFVIGNVAAQLVGQTPKFGPRREEAQQVISDIIGAPCSLSATTTDHMGFTGRSEGRASVATAVVWKV

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=34.8387096774194, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CORGB (A4QH59)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139477.1
- ProteinModelPortal:   A4QH59
- SMR:   A4QH59
- STRING:   A4QH59
- GeneID:   4993937
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2563
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16671; Mature: 16540

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPIIPRVGIATDAHQIEAGKPCWIACLLFEGVDGCEGHSDGDVIAHAIVDALLSASGL
CCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GDLGSFVGVGRPEYDGVSGTQLLKEVRELLESRGFVIGNVAAQLVGQTPKFGPRREEAQQ
CHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCCCHHHHHH
VISDIIGAPCSLSATTTDHMGFTGRSEGRASVATAVVWKV
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEECC
>Mature Secondary Structure 
TNPIIPRVGIATDAHQIEAGKPCWIACLLFEGVDGCEGHSDGDVIAHAIVDALLSASGL
CCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GDLGSFVGVGRPEYDGVSGTQLLKEVRELLESRGFVIGNVAAQLVGQTPKFGPRREEAQQ
CHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCCCHHHHHH
VISDIIGAPCSLSATTTDHMGFTGRSEGRASVATAVVWKV
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA