Click here to switch to the map view.

The map label for this gene is ksdD [H]

Identifier: 145296630

GI number: 145296630

Start: 2803968

End: 2805659

Strand: Reverse

Name: ksdD [H]

Synonym: cgR_2538

Alternate gene names: 145296630

Gene position: 2805659-2803968 (Counterclockwise)

Preceding gene: 145296631

Following gene: 145296626

Centisome position: 84.66

GC content: 55.97

Gene sequence:

>1692_bases
ATGAAACACCAATATGATGTCATCGTCGTCGGTTCCGGTGCTGGCGGATTATCAGCTGCAGTCAGTGCAGCTTACGGCGG
TAAGAAAGTCGCTGTAATTGAAAAGGCCTCAGTACTCGGTGGAGCCACCACCTGGTCCGGCGGTTGGGCGTGGACTCCTG
GAACCAGCTTTGCGCGCAAAGACGGAGTGGTGGAATCCAAAGAAGAATTCCAAACCTACCTGCAAGCGGTAGTGGGGGAG
TACTACCAAGAAGACAACATCTCCGCCTTCTTGGACACGGCCCCTGAAATGGTCGATTTCTTTGAAAACAACACCGACCT
GCAGTGGACCCCCGGCGCGAAAATCAACGACATCTACGGCAACCTCCCCGGTGCTGGCACTGGCCACCGCTCCGTTGGGC
CAAAACCATTGAACGGACGCAAAGTACCCAAGAGTGTCCTTCCAAAACTGCGCCACCAGCTGTATGAAACCTCCTTCCTG
GGAATGGGCATCATGGCTGGACCTGACCTGACGAAATTCCTCTCCGCTTCACAGTTCGATCCACGCGGTTGGGTACATGC
CGCCAGGCGCGTCATCGTGCACATGTGGGACATGGTCGTGCACAAACGCAACATGCAGATGGTTAACGGTGCGGCACTCA
CCGCTCGATTGGCTACCTCGGCAGACAAGCTGGGCGTTGATCTCCTGGTCAATCACTCCGCAGTGTCGTTGAATTACAAA
AACGACCGCGTTACCGGCGTGAAAGTACAAACCCCACAGGGTTTGGTAGATTTCGAAGCCACTGCCGGCGTCGTGCTCGC
CACTGGTGGATTCCCCAACAACGTTGACCTGCGCAAGGAACTCTTCCCACGCACCCCGTCAGGTCAAGAACACTGGACCC
TCGCGCCAGCAGAAACCACCGGCGACGGACTATCCATGGCTCGGGAAATCGGTGCAGGTTTTGTCAACGACCTGAAATCC
CCAGCAGCATGGTGCCCTGTTTCATTGGTCCCATACTTCAACGGAAAAGTCGGCACCTTCCCCCACATCATGGACCGCGC
AAAACCAGGCTCCATCGGTGTTGTCTCCACAGGTAAACGATTTGTCAATGAAGCCAACGGCTACTACGACTACGTCGATG
CCATGCTGAAAGCAGCCCCCGAAGGCGAACCAGTTGCATCCTGGCAGATCGCCGACTCCACCTTCGTGCGCAAATACCCA
CTGGGAATGGCCAAGCCACTACCAGTACCACTCACGCCATACCTGCGATCCGGATACTTGGTAAAGGGCAAAACCATCGA
GGAACTAGCCCAAAAATGCGGCATCGATCCTGCAGGTCTGCGCGCCACCGTCACAGAATTCAATGAAAACGCGCGCCGGG
GCGAAGACCCAGAGTTTGGTCGCGGATCCACCGCATTCAACAAGTATGGCGGCGACCCCAAGATCGGCCCCAACCCATCA
CTCGCACCAATCGAGAAGGGACCGTTCTACGCAGTAAAAGTTGTCCCTGGCTCATTTGGCACCTTCGATGGAATCAAAGC
CGATGGAAAATCACGAGTTCTCCGCGATGACGACACCATCATTGATGGCCTTTACACCGCCGGCAACGACCGTGCCTCCA
TCATGGGTGGTTTCTACCCAGCAGGTGGCATCAACCTTGGCCCAGCGCTGGCATTCGGATACATCGCGGGCAAAGAACTG
GCTAACTCCTAA

Upstream 100 bases:

>100_bases
CAACGATGGGGAAGTAGCCTCTCGTGGCGACGCCGGATGGATCAACCACCTCTACAACACCACCCAAGCTCTGTTAGAAA
AAATTGAGGAAGCAGTCTAA

Downstream 100 bases:

>100_bases
ATGTCGATTTCTTAACAGCGATTTCGGTCGGAAAACCGGGGAAAACCGAGCGAAATCGCCGCTGAGAAATTGAGCTTGAG
GTATTGGAACCGTGAAATCG

Product: hypothetical protein

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MKHQYDVIVVGSGAGGLSAAVSAAYGGKKVAVIEKASVLGGATTWSGGWAWTPGTSFARKDGVVESKEEFQTYLQAVVGE
YYQEDNISAFLDTAPEMVDFFENNTDLQWTPGAKINDIYGNLPGAGTGHRSVGPKPLNGRKVPKSVLPKLRHQLYETSFL
GMGIMAGPDLTKFLSASQFDPRGWVHAARRVIVHMWDMVVHKRNMQMVNGAALTARLATSADKLGVDLLVNHSAVSLNYK
NDRVTGVKVQTPQGLVDFEATAGVVLATGGFPNNVDLRKELFPRTPSGQEHWTLAPAETTGDGLSMAREIGAGFVNDLKS
PAAWCPVSLVPYFNGKVGTFPHIMDRAKPGSIGVVSTGKRFVNEANGYYDYVDAMLKAAPEGEPVASWQIADSTFVRKYP
LGMAKPLPVPLTPYLRSGYLVKGKTIEELAQKCGIDPAGLRATVTEFNENARRGEDPEFGRGSTAFNKYGGDPKIGPNPS
LAPIEKGPFYAVKVVPGSFGTFDGIKADGKSRVLRDDDTIIDGLYTAGNDRASIMGGFYPAGGINLGPALAFGYIAGKEL
ANS

Sequences:

>Translated_563_residues
MKHQYDVIVVGSGAGGLSAAVSAAYGGKKVAVIEKASVLGGATTWSGGWAWTPGTSFARKDGVVESKEEFQTYLQAVVGE
YYQEDNISAFLDTAPEMVDFFENNTDLQWTPGAKINDIYGNLPGAGTGHRSVGPKPLNGRKVPKSVLPKLRHQLYETSFL
GMGIMAGPDLTKFLSASQFDPRGWVHAARRVIVHMWDMVVHKRNMQMVNGAALTARLATSADKLGVDLLVNHSAVSLNYK
NDRVTGVKVQTPQGLVDFEATAGVVLATGGFPNNVDLRKELFPRTPSGQEHWTLAPAETTGDGLSMAREIGAGFVNDLKS
PAAWCPVSLVPYFNGKVGTFPHIMDRAKPGSIGVVSTGKRFVNEANGYYDYVDAMLKAAPEGEPVASWQIADSTFVRKYP
LGMAKPLPVPLTPYLRSGYLVKGKTIEELAQKCGIDPAGLRATVTEFNENARRGEDPEFGRGSTAFNKYGGDPKIGPNPS
LAPIEKGPFYAVKVVPGSFGTFDGIKADGKSRVLRDDDTIIDGLYTAGNDRASIMGGFYPAGGINLGPALAFGYIAGKEL
ANS
>Mature_563_residues
MKHQYDVIVVGSGAGGLSAAVSAAYGGKKVAVIEKASVLGGATTWSGGWAWTPGTSFARKDGVVESKEEFQTYLQAVVGE
YYQEDNISAFLDTAPEMVDFFENNTDLQWTPGAKINDIYGNLPGAGTGHRSVGPKPLNGRKVPKSVLPKLRHQLYETSFL
GMGIMAGPDLTKFLSASQFDPRGWVHAARRVIVHMWDMVVHKRNMQMVNGAALTARLATSADKLGVDLLVNHSAVSLNYK
NDRVTGVKVQTPQGLVDFEATAGVVLATGGFPNNVDLRKELFPRTPSGQEHWTLAPAETTGDGLSMAREIGAGFVNDLKS
PAAWCPVSLVPYFNGKVGTFPHIMDRAKPGSIGVVSTGKRFVNEANGYYDYVDAMLKAAPEGEPVASWQIADSTFVRKYP
LGMAKPLPVPLTPYLRSGYLVKGKTIEELAQKCGIDPAGLRATVTEFNENARRGEDPEFGRGSTAFNKYGGDPKIGPNPS
LAPIEKGPFYAVKVVPGSFGTFDGIKADGKSRVLRDDDTIIDGLYTAGNDRASIMGGFYPAGGINLGPALAFGYIAGKEL
ANS

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 1,4- androstadiene-3,17-dione (ADD) from 4-androstene-3,17-dione (AD) to [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 60176; Mature: 60176

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHQYDVIVVGSGAGGLSAAVSAAYGGKKVAVIEKASVLGGATTWSGGWAWTPGTSFARK
CCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCCCEEECCCCCHHHH
DGVVESKEEFQTYLQAVVGEYYQEDNISAFLDTAPEMVDFFENNTDLQWTPGAKINDIYG
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCCCEEECCCCCEEHHCC
NLPGAGTGHRSVGPKPLNGRKVPKSVLPKLRHQLYETSFLGMGIMAGPDLTKFLSASQFD
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHCCCCC
PRGWVHAARRVIVHMWDMVVHKRNMQMVNGAALTARLATSADKLGVDLLVNHSAVSLNYK
CHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHCHHHCCEEEEEECEEEEEEEC
NDRVTGVKVQTPQGLVDFEATAGVVLATGGFPNNVDLRKELFPRTPSGQEHWTLAPAETT
CCCEEEEEEECCCCCEEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCEEECCCCCC
GDGLSMAREIGAGFVNDLKSPAAWCPVSLVPYFNGKVGTFPHIMDRAKPGSIGVVSTGKR
CCHHHHHHHHCCHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCEEEEECCHH
FVNEANGYYDYVDAMLKAAPEGEPVASWQIADSTFVRKYPLGMAKPLPVPLTPYLRSGYL
HHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCHHHHCCEE
VKGKTIEELAQKCGIDPAGLRATVTEFNENARRGEDPEFGRGSTAFNKYGGDPKIGPNPS
EECCHHHHHHHHHCCCCCCCEEEHHHHCCHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCC
LAPIEKGPFYAVKVVPGSFGTFDGIKADGKSRVLRDDDTIIDGLYTAGNDRASIMGGFYP
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEECCCCHHHHHEEECCCCCHHEECCCCC
AGGINLGPALAFGYIAGKELANS
CCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKHQYDVIVVGSGAGGLSAAVSAAYGGKKVAVIEKASVLGGATTWSGGWAWTPGTSFARK
CCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCCCEEECCCCCHHHH
DGVVESKEEFQTYLQAVVGEYYQEDNISAFLDTAPEMVDFFENNTDLQWTPGAKINDIYG
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHCCCCCEEECCCCCEEHHCC
NLPGAGTGHRSVGPKPLNGRKVPKSVLPKLRHQLYETSFLGMGIMAGPDLTKFLSASQFD
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHCCCCC
PRGWVHAARRVIVHMWDMVVHKRNMQMVNGAALTARLATSADKLGVDLLVNHSAVSLNYK
CHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHCHHHCCEEEEEECEEEEEEEC
NDRVTGVKVQTPQGLVDFEATAGVVLATGGFPNNVDLRKELFPRTPSGQEHWTLAPAETT
CCCEEEEEEECCCCCEEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCEEECCCCCC
GDGLSMAREIGAGFVNDLKSPAAWCPVSLVPYFNGKVGTFPHIMDRAKPGSIGVVSTGKR
CCHHHHHHHHCCHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCEEEEECCHH
FVNEANGYYDYVDAMLKAAPEGEPVASWQIADSTFVRKYPLGMAKPLPVPLTPYLRSGYL
HHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCCCCCCCCCCCCHHHHCCEE
VKGKTIEELAQKCGIDPAGLRATVTEFNENARRGEDPEFGRGSTAFNKYGGDPKIGPNPS
EECCHHHHHHHHHCCCCCCCEEEHHHHCCHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCC
LAPIEKGPFYAVKVVPGSFGTFDGIKADGKSRVLRDDDTIIDGLYTAGNDRASIMGGFYP
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEECCCCHHHHHEEECCCCCHHEECCCCC
AGGINLGPALAFGYIAGKELANS
CCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA