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The map label for this gene is fixJ [H]

Identifier: 145296385

GI number: 145296385

Start: 2532785

End: 2533402

Strand: Reverse

Name: fixJ [H]

Synonym: cgR_2299

Alternate gene names: 145296385

Gene position: 2533402-2532785 (Counterclockwise)

Preceding gene: 145296386

Following gene: 145296378

Centisome position: 76.44

GC content: 46.93

Gene sequence:

>618_bases
ATGGATAAGATTGATCCCGTCGTCTACATTGTGGATGATGACGTGGAAGTGTGCGAATCGGTTGCTTGGCTATTAGAAAG
CGCGAGCATCGAAAGCACAATTTGTCATAGCGCTCAGGAACTCTTGGACTCGTTTGATGACACTAAGCCAGCTTGTTTGA
TCCTTGATGTTCGTATGCCATCTATGAGCGGGATTCGTCTCCAGGAAAAACTAAATGAGCTTTCTCCACATGTTGTAGTG
ATCTTTGTATCTGCCCATGGTGATATTCGGATGTCGGTCGACACCATTAAGGCCGGAGCTCAGGATTTTCTAGAGAAACC
CTATGATTCTCAACGACTCCTTGACGCGGTACAACTTGGAATTGAATCGGCAGAACGTCGATTCATTGAATACAGCTTGG
CGGCAGAAATTCGCCGAAAAATCGCACTTCTAACAATTCGAGAAAAGGAAGTGCTTCGTTTGGTCGTTGAAGGGCTACCG
AGTCAGAACATTGGCAGGCGACTGGGCATGAGCGTGAAGACCGTCGATGTACATCGCACCCGTATTAAGTCCAAGACCGA
TGCAGACAGTGTTGGGACTTTCGTCCGAGATGTGTTGAGATTCGGAGTAGAAATATAG

Upstream 100 bases:

>100_bases
AGTGATTTTGGCATGAACTACTGGGTCGCCGAAGCTTCTTGGGATTGAGATTTAAGTGAATATCGCGATGAAACAGCAGG
TGGGGAAAGATTTTCAACCC

Downstream 100 bases:

>100_bases
AGCTCCAATCCTATTCATAAGGGCCACATGAACCTTTGGTGTTCATGTGGCCCTTATTTGTCCCGCGACTTTGTGATGGC
TCTATTAATACTGACTTCTT

Product: hypothetical protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MDKIDPVVYIVDDDVEVCESVAWLLESASIESTICHSAQELLDSFDDTKPACLILDVRMPSMSGIRLQEKLNELSPHVVV
IFVSAHGDIRMSVDTIKAGAQDFLEKPYDSQRLLDAVQLGIESAERRFIEYSLAAEIRRKIALLTIREKEVLRLVVEGLP
SQNIGRRLGMSVKTVDVHRTRIKSKTDADSVGTFVRDVLRFGVEI

Sequences:

>Translated_205_residues
MDKIDPVVYIVDDDVEVCESVAWLLESASIESTICHSAQELLDSFDDTKPACLILDVRMPSMSGIRLQEKLNELSPHVVV
IFVSAHGDIRMSVDTIKAGAQDFLEKPYDSQRLLDAVQLGIESAERRFIEYSLAAEIRRKIALLTIREKEVLRLVVEGLP
SQNIGRRLGMSVKTVDVHRTRIKSKTDADSVGTFVRDVLRFGVEI
>Mature_205_residues
MDKIDPVVYIVDDDVEVCESVAWLLESASIESTICHSAQELLDSFDDTKPACLILDVRMPSMSGIRLQEKLNELSPHVVV
IFVSAHGDIRMSVDTIKAGAQDFLEKPYDSQRLLDAVQLGIESAERRFIEYSLAAEIRRKIALLTIREKEVLRLVVEGLP
SQNIGRRLGMSVKTVDVHRTRIKSKTDADSVGTFVRDVLRFGVEI

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=107, Percent_Identity=33.6448598130841, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786747, Length=151, Percent_Identity=26.4900662251656, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790437, Length=123, Percent_Identity=29.2682926829268, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22983; Mature: 22983

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIDPVVYIVDDDVEVCESVAWLLESASIESTICHSAQELLDSFDDTKPACLILDVRMP
CCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
SMSGIRLQEKLNELSPHVVVIFVSAHGDIRMSVDTIKAGAQDFLEKPYDSQRLLDAVQLG
CCCCCHHHHHHHHCCCEEEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IESAERRFIEYSLAAEIRRKIALLTIREKEVLRLVVEGLPSQNIGRRLGMSVKTVDVHRT
HHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHCCCCCCHHHHHCCCEEEEHHHHH
RIKSKTDADSVGTFVRDVLRFGVEI
HHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKIDPVVYIVDDDVEVCESVAWLLESASIESTICHSAQELLDSFDDTKPACLILDVRMP
CCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
SMSGIRLQEKLNELSPHVVVIFVSAHGDIRMSVDTIKAGAQDFLEKPYDSQRLLDAVQLG
CCCCCHHHHHHHHCCCEEEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IESAERRFIEYSLAAEIRRKIALLTIREKEVLRLVVEGLPSQNIGRRLGMSVKTVDVHRT
HHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHCCCCCCHHHHHCCCEEEEHHHHH
RIKSKTDADSVGTFVRDVLRFGVEI
HHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]