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The map label for this gene is fixL [H]

Identifier: 145296378

GI number: 145296378

Start: 2525518

End: 2526816

Strand: Reverse

Name: fixL [H]

Synonym: cgR_2292

Alternate gene names: 145296378

Gene position: 2526816-2525518 (Counterclockwise)

Preceding gene: 145296385

Following gene: 145296370

Centisome position: 76.24

GC content: 48.11

Gene sequence:

>1299_bases
ATGGGCATTTCTTGGCTGCAGATGGCCGTCGTAGAAGGTTCAAGTCGTAAGTTGTGGAAGTACCGGGACCGAAACGGGAT
GGACTTTCTGACTGAAGCTACGGCCTCTATCTATGAGCTTGAACAAGCCGGCGAGGTATTAGTAGTTAAGTTTCGAATTC
TCGGTGGTACCGAAGCTCCACCTCAGCCGTCCACGTGGGTTGAAGAAACTCTGGATAGGTTGATGCGGCACACCTCATCG
GGAATTTTGTTGCTAAATAATGAAAACCAAGTAAAAGATGCTTCTCCGCTTGCGGCGAGGCTTTTTGGCCACTCAGTCGC
AGATATTTTAAATAAAGACCTCAATGAATTGGGGGTGTCTGACGTTGATTTTGATTCCGACGAGGCTAAATATCAGTTGA
TGAAACCTGAGGGGTCGATGGATATTCGTATCAAGGTTTCTGATCGGTATGGCACGTTACGTTGGTTGGCTGGCTTCTTG
GATAACGTCGTAGATAAGGACAGGCGATTTCGTGTTCTCACGGTTCGAGATATTTCGGAGAAGGTTGAGTGGGAGAAGCG
AAATGCTCATCAGATGGCTAATTTGCAGTATTTGAGCAGGTATAACGCCATGGGGGACATGGCGATGATCCTTGCTCATG
AGCTGGGACAGCCTCTTGCTGCCGCGACTAATTACCTTGGCGGGGTACTCAAGCGAATTCCAGACGAAGGAACGGAAGAG
AGACCAGAACAGCGGAGTGTCTCCTATGGCATTGAACAGGCAAAGAAGCAGTTGGAGAGAGCTTCTGAGATCGTGTCCTC
AGTAAAGAGGTATGTGAGGAGAATTGAAAGTGCCACCTCCGTATTCGATCTGAATCGGGAGGTGGAGGAGAGTCTGTACT
TTGCTCGATTAAGAGCTGAAGAAAAGGGAGTGCTTATTCTCGATGACCTACTTCCGGATGAGCTTCTAATTGAGGGGGAG
AGCGTTCTGATTGGTCAGGTGATCATTAATATTTGTATGAATGCTCTTGAAGAAGTCATTTTGCCAACGACTGAGGAAAA
AGTGATGGAGTTGCGCACCGAAAGTGACGGCAACACAGTTTCCATCCTTGTTTGTGACCAAGGCCGAGGCATAGAAGGCG
TGCCTGCAGATCGTTTAGCAGCAGGTGCTTTTAGCTCAAAAGAAGACGGCTCTGGCATTGGGCTGATTATTTCTGAGCAT
ATTGTGCAGCGACACGGTGGGCACATTACTTATGAACCCAATCAGCCACGTGGAACGAAGGTTCGTATCACGCTGCCGTT
GGTTTCCTCAAAAAGTTAG

Upstream 100 bases:

>100_bases
CAACCGCAATGCATGTGAAGCTTTCAAGTATTCCGTTGAAGAATTGCGGGCACTCAAGGCGCATCATATGAGTTCCCAGG
ATCCTAATTATCGGCGAGAG

Downstream 100 bases:

>100_bases
CTAAGCCCGTTTTTTGTTTTCTAGAAGCTTGCTATCCAGCAGCCATCCGACGAGCCATCCGAAGACAATTCCCCAAAACG
GGGCGGTGATTCCGAGGATG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGISWLQMAVVEGSSRKLWKYRDRNGMDFLTEATASIYELEQAGEVLVVKFRILGGTEAPPQPSTWVEETLDRLMRHTSS
GILLLNNENQVKDASPLAARLFGHSVADILNKDLNELGVSDVDFDSDEAKYQLMKPEGSMDIRIKVSDRYGTLRWLAGFL
DNVVDKDRRFRVLTVRDISEKVEWEKRNAHQMANLQYLSRYNAMGDMAMILAHELGQPLAAATNYLGGVLKRIPDEGTEE
RPEQRSVSYGIEQAKKQLERASEIVSSVKRYVRRIESATSVFDLNREVEESLYFARLRAEEKGVLILDDLLPDELLIEGE
SVLIGQVIINICMNALEEVILPTTEEKVMELRTESDGNTVSILVCDQGRGIEGVPADRLAAGAFSSKEDGSGIGLIISEH
IVQRHGGHITYEPNQPRGTKVRITLPLVSSKS

Sequences:

>Translated_432_residues
MGISWLQMAVVEGSSRKLWKYRDRNGMDFLTEATASIYELEQAGEVLVVKFRILGGTEAPPQPSTWVEETLDRLMRHTSS
GILLLNNENQVKDASPLAARLFGHSVADILNKDLNELGVSDVDFDSDEAKYQLMKPEGSMDIRIKVSDRYGTLRWLAGFL
DNVVDKDRRFRVLTVRDISEKVEWEKRNAHQMANLQYLSRYNAMGDMAMILAHELGQPLAAATNYLGGVLKRIPDEGTEE
RPEQRSVSYGIEQAKKQLERASEIVSSVKRYVRRIESATSVFDLNREVEESLYFARLRAEEKGVLILDDLLPDELLIEGE
SVLIGQVIINICMNALEEVILPTTEEKVMELRTESDGNTVSILVCDQGRGIEGVPADRLAAGAFSSKEDGSGIGLIISEH
IVQRHGGHITYEPNQPRGTKVRITLPLVSSKS
>Mature_431_residues
GISWLQMAVVEGSSRKLWKYRDRNGMDFLTEATASIYELEQAGEVLVVKFRILGGTEAPPQPSTWVEETLDRLMRHTSSG
ILLLNNENQVKDASPLAARLFGHSVADILNKDLNELGVSDVDFDSDEAKYQLMKPEGSMDIRIKVSDRYGTLRWLAGFLD
NVVDKDRRFRVLTVRDISEKVEWEKRNAHQMANLQYLSRYNAMGDMAMILAHELGQPLAAATNYLGGVLKRIPDEGTEER
PEQRSVSYGIEQAKKQLERASEIVSSVKRYVRRIESATSVFDLNREVEESLYFARLRAEEKGVLILDDLLPDELLIEGES
VLIGQVIINICMNALEEVILPTTEEKVMELRTESDGNTVSILVCDQGRGIEGVPADRLAAGAFSSKEDGSGIGLIISEHI
VQRHGGHITYEPNQPRGTKVRITLPLVSSKS

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=228, Percent_Identity=25.4385964912281, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48332; Mature: 48200

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISWLQMAVVEGSSRKLWKYRDRNGMDFLTEATASIYELEQAGEVLVVKFRILGGTEAP
CCCHHHHHHHHCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCC
PQPSTWVEETLDRLMRHTSSGILLLNNENQVKDASPLAARLFGHSVADILNKDLNELGVS
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DVDFDSDEAKYQLMKPEGSMDIRIKVSDRYGTLRWLAGFLDNVVDKDRRFRVLTVRDISE
CCCCCCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
KVEWEKRNAHQMANLQYLSRYNAMGDMAMILAHELGQPLAAATNYLGGVLKRIPDEGTEE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCC
RPEQRSVSYGIEQAKKQLERASEIVSSVKRYVRRIESATSVFDLNREVEESLYFARLRAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
EKGVLILDDLLPDELLIEGESVLIGQVIINICMNALEEVILPTTEEKVMELRTESDGNTV
CCCEEEECCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEE
SILVCDQGRGIEGVPADRLAAGAFSSKEDGSGIGLIISEHIVQRHGGHITYEPNQPRGTK
EEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCEEEHHHHHHHHHCCCEEEECCCCCCCCE
VRITLPLVSSKS
EEEEEEECCCCC
>Mature Secondary Structure 
GISWLQMAVVEGSSRKLWKYRDRNGMDFLTEATASIYELEQAGEVLVVKFRILGGTEAP
CCHHHHHHHHCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCC
PQPSTWVEETLDRLMRHTSSGILLLNNENQVKDASPLAARLFGHSVADILNKDLNELGVS
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DVDFDSDEAKYQLMKPEGSMDIRIKVSDRYGTLRWLAGFLDNVVDKDRRFRVLTVRDISE
CCCCCCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHH
KVEWEKRNAHQMANLQYLSRYNAMGDMAMILAHELGQPLAAATNYLGGVLKRIPDEGTEE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCC
RPEQRSVSYGIEQAKKQLERASEIVSSVKRYVRRIESATSVFDLNREVEESLYFARLRAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
EKGVLILDDLLPDELLIEGESVLIGQVIINICMNALEEVILPTTEEKVMELRTESDGNTV
CCCEEEECCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEE
SILVCDQGRGIEGVPADRLAAGAFSSKEDGSGIGLIISEHIVQRHGGHITYEPNQPRGTK
EEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCEEEHHHHHHHHHCCCEEEECCCCCCCCE
VRITLPLVSSKS
EEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]