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The map label for this gene is hscA [H]

Identifier: 145296345

GI number: 145296345

Start: 2490455

End: 2491909

Strand: Direct

Name: hscA [H]

Synonym: cgR_2260

Alternate gene names: 145296345

Gene position: 2490455-2491909 (Clockwise)

Preceding gene: 145296339

Following gene: 145296348

Centisome position: 75.15

GC content: 55.88

Gene sequence:

>1455_bases
ATGCGTTTTGGACTTGACTTGGGAACTACCCGCACAATCGCGGCCGCCGTGGACCGTGGAAACTATCCCATCGTCACTGT
GGAAGATTCTTTAGGCGACACCCACGATTTCATCCCATCTGTGGTGGCCCTCAAGGCAGATAGGATTGTCGCGGGTTGGG
ATGCTATTGAGGTTGGGCAGGACCACCCTTCCTTCGTGCGTTCTTTCAAACGCCTACTCTCTGAACCCAACGTCACGGAA
GCCACCCCGGTCTACTTGGGCGATCATGTACACCCTTTGGGCGCCGTCCTGGAGGCTTTTGCGGAAAACGTGGTCACTGC
GCTGCGTGCATTTCAGACGCAATTGGGAGATACCTCCCCGATCGAAGTAGTCATTGGTGTGCCCGCCAACTCCCACAGCG
CCCAACGTCTGCTCACCATGTCCGCCTTCAGCGCCACAGGCATCACCGTTGTCGGTTTGGTCAATGAGCCCAGCGCCGCA
GCTTTCGAGTACACCCACCGCCACGCCCGCACCTTAAACTCCAAGCGCCAAGCCATCGTGGTTTACGACTTGGGAGGCGG
AACATTTGACTCCTCGCTCATCCGCATCGACGGCACCCACCACGAGGTTGTGTCCTCCATTGGCATTTCACGCCTTGGTG
GCGATGATTTCGATGAAATCCTCCTCCAATGCGCGCTCAAGGTCGCAGGCAGGCAGCACGATGCGTTTGGCAAGCGTGCT
AAAAACACGCTTCTCGACGAATCCCGCAACGCGAAGGAAGCTCTCGTTCCGCAATCCCGTCGCTTGGTTCTAGAAATTGG
CGACGACGACATCACCGTTCCAGTGAACAAGTTCTACGAGGCTGCCACTCCCCTGGTGGAAAAATCCTTGTCCATCATGG
AACCCCTCATCGGTGTCGATGATCTTAAAGATTCCGACATCGCAGGCATCTACCTTGTTGGTGGAGGATCCTCACTTCCA
CTTGTTTCCCGACTGCTCCGCGAACGTTTCGGCCGACGTGTTCACCGCTCTCCCTTCCCTTCAGGTTCCACAGCAGTGGG
TCTGGCCATCGCTGCTGATCCTTCTTCTGGTTTCCATCTAAGGGACCGGGTCGCACGTGGCATCGGTGTATTCCGTGAGC
ACGATTCCGGCCGCGCCGTCAGCTTCGACCCGTTGATCGCCCCGGACACTGATTCCGCGACCGTAGCGAAACGCCACTAC
AGGGCAGTGCACAACATTGGTTGGTTCAGGTTCGTGGAATATTCCACCGTGTCTGAGGATGGCAGCCCCGGAGATATCTC
ACTGCTCAGTGAAATCAAGATCCCTTTTGATAACTCCATCACCGATGTGGATGCTACTGAGATTTCACGTTTCGATGGCC
CAGAAGTAGAAGAAACCATCACCGTCAATGACAACGGTGTGGCTTCCATTTCCATCAAAGTGCTCGGTGGCGTTACCGTC
GAGCACACAATCTAA

Upstream 100 bases:

>100_bases
GCGCCCTCATTGAACATGCAAAACTAATGCTTTTGGGGGGGTATGCATAAATTCGTTTCGTTCCACTGCACAGGCCAAAA
ATGCTGCTAGGGTCAAGTTC

Downstream 100 bases:

>100_bases
TCACCATTTTGGTGCTGGTGGAGTCCAGGTCTGCATTGCCTCTAGCAGGGCCTTCGGGTCGGATTCCACGATGAGGCATT
CAAAGAAGTCTCGTTTGATA

Product: hypothetical protein

Products: NA

Alternate protein names: Hsc66 [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MRFGLDLGTTRTIAAAVDRGNYPIVTVEDSLGDTHDFIPSVVALKADRIVAGWDAIEVGQDHPSFVRSFKRLLSEPNVTE
ATPVYLGDHVHPLGAVLEAFAENVVTALRAFQTQLGDTSPIEVVIGVPANSHSAQRLLTMSAFSATGITVVGLVNEPSAA
AFEYTHRHARTLNSKRQAIVVYDLGGGTFDSSLIRIDGTHHEVVSSIGISRLGGDDFDEILLQCALKVAGRQHDAFGKRA
KNTLLDESRNAKEALVPQSRRLVLEIGDDDITVPVNKFYEAATPLVEKSLSIMEPLIGVDDLKDSDIAGIYLVGGGSSLP
LVSRLLRERFGRRVHRSPFPSGSTAVGLAIAADPSSGFHLRDRVARGIGVFREHDSGRAVSFDPLIAPDTDSATVAKRHY
RAVHNIGWFRFVEYSTVSEDGSPGDISLLSEIKIPFDNSITDVDATEISRFDGPEVEETITVNDNGVASISIKVLGGVTV
EHTI

Sequences:

>Translated_484_residues
MRFGLDLGTTRTIAAAVDRGNYPIVTVEDSLGDTHDFIPSVVALKADRIVAGWDAIEVGQDHPSFVRSFKRLLSEPNVTE
ATPVYLGDHVHPLGAVLEAFAENVVTALRAFQTQLGDTSPIEVVIGVPANSHSAQRLLTMSAFSATGITVVGLVNEPSAA
AFEYTHRHARTLNSKRQAIVVYDLGGGTFDSSLIRIDGTHHEVVSSIGISRLGGDDFDEILLQCALKVAGRQHDAFGKRA
KNTLLDESRNAKEALVPQSRRLVLEIGDDDITVPVNKFYEAATPLVEKSLSIMEPLIGVDDLKDSDIAGIYLVGGGSSLP
LVSRLLRERFGRRVHRSPFPSGSTAVGLAIAADPSSGFHLRDRVARGIGVFREHDSGRAVSFDPLIAPDTDSATVAKRHY
RAVHNIGWFRFVEYSTVSEDGSPGDISLLSEIKIPFDNSITDVDATEISRFDGPEVEETITVNDNGVASISIKVLGGVTV
EHTI
>Mature_484_residues
MRFGLDLGTTRTIAAAVDRGNYPIVTVEDSLGDTHDFIPSVVALKADRIVAGWDAIEVGQDHPSFVRSFKRLLSEPNVTE
ATPVYLGDHVHPLGAVLEAFAENVVTALRAFQTQLGDTSPIEVVIGVPANSHSAQRLLTMSAFSATGITVVGLVNEPSAA
AFEYTHRHARTLNSKRQAIVVYDLGGGTFDSSLIRIDGTHHEVVSSIGISRLGGDDFDEILLQCALKVAGRQHDAFGKRA
KNTLLDESRNAKEALVPQSRRLVLEIGDDDITVPVNKFYEAATPLVEKSLSIMEPLIGVDDLKDSDIAGIYLVGGGSSLP
LVSRLLRERFGRRVHRSPFPSGSTAVGLAIAADPSSGFHLRDRVARGIGVFREHDSGRAVSFDPLIAPDTDSATVAKRHY
RAVHNIGWFRFVEYSTVSEDGSPGDISLLSEIKIPFDNSITDVDATEISRFDGPEVEETITVNDNGVASISIKVLGGVTV
EHTI

Specific function: Chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by hscB. Involved in the maturation of iscU [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI16507237, Length=391, Percent_Identity=29.4117647058824, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI124256496, Length=382, Percent_Identity=28.5340314136126, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI167466173, Length=381, Percent_Identity=28.0839895013123, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI194248072, Length=381, Percent_Identity=28.0839895013123, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI34419635, Length=241, Percent_Identity=33.6099585062241, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI24234686, Length=384, Percent_Identity=27.34375, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI5729877, Length=384, Percent_Identity=27.34375, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI13676857, Length=239, Percent_Identity=31.3807531380753, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI24234688, Length=383, Percent_Identity=28.1984334203655, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI42544159, Length=272, Percent_Identity=26.8382352941176, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI38327039, Length=254, Percent_Identity=24.8031496062992, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788875, Length=388, Percent_Identity=30.4123711340206, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786870, Length=360, Percent_Identity=29.1666666666667, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786196, Length=389, Percent_Identity=29.305912596401, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17534771, Length=386, Percent_Identity=29.0155440414508, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17568549, Length=245, Percent_Identity=35.1020408163265, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71984444, Length=435, Percent_Identity=28.5057471264368, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17534015, Length=259, Percent_Identity=32.046332046332, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17534013, Length=259, Percent_Identity=32.046332046332, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17541098, Length=252, Percent_Identity=30.5555555555556, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17562024, Length=388, Percent_Identity=27.5773195876289, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17507981, Length=246, Percent_Identity=31.7073170731707, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322426, Length=383, Percent_Identity=29.242819843342, Blast_Score=116, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6319396, Length=267, Percent_Identity=29.9625468164794, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6320950, Length=265, Percent_Identity=29.811320754717, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320805, Length=248, Percent_Identity=32.258064516129, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323004, Length=240, Percent_Identity=30.4166666666667, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6319314, Length=240, Percent_Identity=30.4166666666667, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319972, Length=239, Percent_Identity=31.3807531380753, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324120, Length=239, Percent_Identity=31.3807531380753, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322505, Length=249, Percent_Identity=30.9236947791165, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323401, Length=249, Percent_Identity=28.1124497991968, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24641406, Length=388, Percent_Identity=29.1237113402062, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24641408, Length=388, Percent_Identity=29.1237113402062, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24641402, Length=388, Percent_Identity=29.1237113402062, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24641404, Length=388, Percent_Identity=29.1237113402062, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24647034, Length=241, Percent_Identity=32.3651452282158, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24647038, Length=241, Percent_Identity=32.3651452282158, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24647036, Length=241, Percent_Identity=32.3651452282158, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17737967, Length=241, Percent_Identity=32.3651452282158, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571721, Length=241, Percent_Identity=32.3651452282158, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571719, Length=241, Percent_Identity=32.3651452282158, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571678, Length=385, Percent_Identity=28.8311688311688, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24646324, Length=385, Percent_Identity=28.8311688311688, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24646322, Length=385, Percent_Identity=28.8311688311688, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24646318, Length=385, Percent_Identity=28.8311688311688, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17738165, Length=384, Percent_Identity=28.90625, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24646137, Length=385, Percent_Identity=28.8311688311688, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646135, Length=385, Percent_Identity=28.8311688311688, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17647515, Length=390, Percent_Identity=29.4871794871795, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24663992, Length=271, Percent_Identity=32.8413284132841, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24663988, Length=271, Percent_Identity=32.8413284132841, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17737941, Length=381, Percent_Identity=26.7716535433071, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24653595, Length=251, Percent_Identity=27.8884462151394, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24664047, Length=515, Percent_Identity=23.8834951456311, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24664044, Length=515, Percent_Identity=23.8834951456311, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21357475, Length=515, Percent_Identity=23.8834951456311, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126
- InterPro:   IPR010236 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 52120; Mature: 52120

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00329 HSP70_2 ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFGLDLGTTRTIAAAVDRGNYPIVTVEDSLGDTHDFIPSVVALKADRIVAGWDAIEVGQ
CCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCEECCC
DHPSFVRSFKRLLSEPNVTEATPVYLGDHVHPLGAVLEAFAENVVTALRAFQTQLGDTSP
CCHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IEVVIGVPANSHSAQRLLTMSAFSATGITVVGLVNEPSAAAFEYTHRHARTLNSKRQAIV
EEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEE
VYDLGGGTFDSSLIRIDGTHHEVVSSIGISRLGGDDFDEILLQCALKVAGRQHDAFGKRA
EEECCCCCCCCCEEEECCCHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
KNTLLDESRNAKEALVPQSRRLVLEIGDDDITVPVNKFYEAATPLVEKSLSIMEPLIGVD
HHHHHCCCCCCHHHHCCCCCEEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCC
DLKDSDIAGIYLVGGGSSLPLVSRLLRERFGRRVHRSPFPSGSTAVGLAIAADPSSGFHL
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHH
RDRVARGIGVFREHDSGRAVSFDPLIAPDTDSATVAKRHYRAVHNIGWFRFVEYSTVSED
HHHHHHHHHHEEECCCCCEEEECCEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCC
GSPGDISLLSEIKIPFDNSITDVDATEISRFDGPEVEETITVNDNGVASISIKVLGGVTV
CCCCCEEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCEEEEEEEEEECEEE
EHTI
EECC
>Mature Secondary Structure
MRFGLDLGTTRTIAAAVDRGNYPIVTVEDSLGDTHDFIPSVVALKADRIVAGWDAIEVGQ
CCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCEECCC
DHPSFVRSFKRLLSEPNVTEATPVYLGDHVHPLGAVLEAFAENVVTALRAFQTQLGDTSP
CCHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IEVVIGVPANSHSAQRLLTMSAFSATGITVVGLVNEPSAAAFEYTHRHARTLNSKRQAIV
EEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEE
VYDLGGGTFDSSLIRIDGTHHEVVSSIGISRLGGDDFDEILLQCALKVAGRQHDAFGKRA
EEECCCCCCCCCEEEECCCHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
KNTLLDESRNAKEALVPQSRRLVLEIGDDDITVPVNKFYEAATPLVEKSLSIMEPLIGVD
HHHHHCCCCCCHHHHCCCCCEEEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCC
DLKDSDIAGIYLVGGGSSLPLVSRLLRERFGRRVHRSPFPSGSTAVGLAIAADPSSGFHL
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHH
RDRVARGIGVFREHDSGRAVSFDPLIAPDTDSATVAKRHYRAVHNIGWFRFVEYSTVSED
HHHHHHHHHHEEECCCCCEEEECCEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCC
GSPGDISLLSEIKIPFDNSITDVDATEISRFDGPEVEETITVNDNGVASISIKVLGGVTV
CCCCCEEEEEEECCCCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCEEEEEEEEEECEEE
EHTI
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA