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The map label for this gene is dnaG [H]

Identifier: 145296223

GI number: 145296223

Start: 2361502

End: 2363403

Strand: Reverse

Name: dnaG [H]

Synonym: cgR_2140

Alternate gene names: 145296223

Gene position: 2363403-2361502 (Counterclockwise)

Preceding gene: 145296226

Following gene: 145296218

Centisome position: 71.31

GC content: 54.21

Gene sequence:

>1902_bases
ATGGTCATGGCAAAGGGACGTATTCCGGAAAGTGACATTCAGGCAATCCGCGAGCGCACCCCGATCGAGGAGATCGTGGG
TGATTATGTGCAGCTGAAATCGGCAGGTGCTGATTCGCTCAAGGGGCTTTCTCCCTTTAAAGATGAAAAAACACCATCGT
TCCATGTGCGCCCCAACCGTGGTTACTACCACTGTTTCTCCACCGGCAAAGGTGGAGATGTGTTCTCCTTCCTCATGGAG
ATGGAACACATCTCTTTCCCTGAGGCTGTGGAAGTGTGTGCGGAGAAAATTGGTTATCAGATCAATTACCAAGGTGGTGG
CCCGGGGCGCCGTGAGGAACCTGGAACTCGCCAGCGCCTTATTTTGGCTAATAAGGCTGCGCACCAGTTTTATCGCGAGC
AATTAGAAACCCCCGAAGCACAACCTGGCAGGGAGTTTTTGCTGCAGCGTGGATTCGGACAGCAGCACATTTATCATTTC
GAATGTGGCTATGCGCCTGCCGGCTGGGATACCTTGACCAAGCATTTGCTGAAGAAGGGCTTTGAGTTCAAAGAATTAGA
AGCTGCCGGTCTAAGCAAGATGGGTAAGCGCGGTCCGATTGATCAGTTCCAGCGCAGGTTGCTGTGGCCGATCAAGAACC
TGTCTGGTGATGTCATTGGTTTTGGCGCCCGCAAGCTTTTTGATGACGACAAAATGGGCAAGTACATGAATACGCCTGAG
ACGTTGTTGTACAAAAAGTCCAAGGTGCTCTTTGGTCTAGATTCTGCAAAGAAGGCCATTGCAGCTGGCCACCAAGCAGT
AGTGGTGGAAGGCTACACCGATGTGATGGCCATGCATGCCGCGGGCATTGAAACTGCTGTGGCATCGTGTGGCACTGCGT
TTGGTGAAGAACACTTGCAGATGCTTCGTCGACTCATGCTGGATGATAACTACTTCCGCGGTGAACTGATTTACACCTTC
GATGGTGATGAGGCCGGCCAGAAGGCCGCCATGCGTGCCTTTGAGGGCGATCAGAAGTTCACAGGACAATCATTTGTGTC
TGTGGCACCCAACGGCATGGATCCGTGTGATCTGCGCCTTGAGCGCGGCGATGCGGCAGTGCGTGATCTTGTAGCACGAC
GTATTCCGATGTTTGAGTTCGTCATCCAATCGATCATCAGCGAATACACCCTCGACACCGTGGAAGGCCGTCTGGCTGCG
CTTCGTCGGGCAGTCCCCATCGTGGCGGATATTCGCGATAAGACACTGCAATCTGAATACGCCCGCCTGCTGTCAGGTTG
GGTCGGCTGGTCTGATCCTTCAGAGGTGCTGCGTCAGGTCCACGAGGAAGCGCGTCGACCCAAGCGCGATAAGAAGCCGG
TGCGTGCAAAGCGTTTCGATCAACCACTCGAGGATCAAAGCTTACGACCCACCATGGCGCTGCCAAATCCGCGGAACCCT
GTGCTGTGGCAGGAGCGGGAATCACTCAAGATCGCCCTGCAATATCCGGAGCTCGCGGGATCGTACTTTGATGGACTGCC
AACCGATAGCTTCACCAACCCTGCCTACCGCATGGTACGCGATGCCATTTCGGCTGCTGGGGGATGTGAACGTGCCCTCG
ATGGCACTGATTGGTTGCCTGCCGTATCGGAAAATATGACTGATATTTTGGGCACGTCGTTGGTGTCAGAGCTAGCGATG
GAACCCATCGAGGTGGAAGCGCAAGACCTGGAATCATATACCGATGGTGTGTTGTCCAGGCTGCAGGAAACACGAGTGGG
CAACCAGATCGCCATCTTGAAAGGACAGCTGCAAAGAATGCGTCCGTCTGAAGATGAGCAAGCCTACAACTCGCTGTTTT
CCGATCTGGTTGCCCTGGAACAGGCGCGCCGGGAGCTATTAGCCCGGGCGTTTAGAGGGTAA

Upstream 100 bases:

>100_bases
CAGCAGCGAGGACAACGATGACTCCGAGCACTCCGCTGAGGGTCTTCTTGCTGTTTTGCATACAACCAATATAGGCACAC
TAAAGGGACGGGCTAAAGTG

Downstream 100 bases:

>100_bases
TTTAGTCCTGGTCTTGCTCGAGGACTTCATGCCCGTCCGTAGTCCGCAGGTTTTTTACTTCCTTCATGCGGGGCTGCGGA
TCCAGCTTGTTGGCAATGGC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MVMAKGRIPESDIQAIRERTPIEEIVGDYVQLKSAGADSLKGLSPFKDEKTPSFHVRPNRGYYHCFSTGKGGDVFSFLME
MEHISFPEAVEVCAEKIGYQINYQGGGPGRREEPGTRQRLILANKAAHQFYREQLETPEAQPGREFLLQRGFGQQHIYHF
ECGYAPAGWDTLTKHLLKKGFEFKELEAAGLSKMGKRGPIDQFQRRLLWPIKNLSGDVIGFGARKLFDDDKMGKYMNTPE
TLLYKKSKVLFGLDSAKKAIAAGHQAVVVEGYTDVMAMHAAGIETAVASCGTAFGEEHLQMLRRLMLDDNYFRGELIYTF
DGDEAGQKAAMRAFEGDQKFTGQSFVSVAPNGMDPCDLRLERGDAAVRDLVARRIPMFEFVIQSIISEYTLDTVEGRLAA
LRRAVPIVADIRDKTLQSEYARLLSGWVGWSDPSEVLRQVHEEARRPKRDKKPVRAKRFDQPLEDQSLRPTMALPNPRNP
VLWQERESLKIALQYPELAGSYFDGLPTDSFTNPAYRMVRDAISAAGGCERALDGTDWLPAVSENMTDILGTSLVSELAM
EPIEVEAQDLESYTDGVLSRLQETRVGNQIAILKGQLQRMRPSEDEQAYNSLFSDLVALEQARRELLARAFRG

Sequences:

>Translated_633_residues
MVMAKGRIPESDIQAIRERTPIEEIVGDYVQLKSAGADSLKGLSPFKDEKTPSFHVRPNRGYYHCFSTGKGGDVFSFLME
MEHISFPEAVEVCAEKIGYQINYQGGGPGRREEPGTRQRLILANKAAHQFYREQLETPEAQPGREFLLQRGFGQQHIYHF
ECGYAPAGWDTLTKHLLKKGFEFKELEAAGLSKMGKRGPIDQFQRRLLWPIKNLSGDVIGFGARKLFDDDKMGKYMNTPE
TLLYKKSKVLFGLDSAKKAIAAGHQAVVVEGYTDVMAMHAAGIETAVASCGTAFGEEHLQMLRRLMLDDNYFRGELIYTF
DGDEAGQKAAMRAFEGDQKFTGQSFVSVAPNGMDPCDLRLERGDAAVRDLVARRIPMFEFVIQSIISEYTLDTVEGRLAA
LRRAVPIVADIRDKTLQSEYARLLSGWVGWSDPSEVLRQVHEEARRPKRDKKPVRAKRFDQPLEDQSLRPTMALPNPRNP
VLWQERESLKIALQYPELAGSYFDGLPTDSFTNPAYRMVRDAISAAGGCERALDGTDWLPAVSENMTDILGTSLVSELAM
EPIEVEAQDLESYTDGVLSRLQETRVGNQIAILKGQLQRMRPSEDEQAYNSLFSDLVALEQARRELLARAFRG
>Mature_633_residues
MVMAKGRIPESDIQAIRERTPIEEIVGDYVQLKSAGADSLKGLSPFKDEKTPSFHVRPNRGYYHCFSTGKGGDVFSFLME
MEHISFPEAVEVCAEKIGYQINYQGGGPGRREEPGTRQRLILANKAAHQFYREQLETPEAQPGREFLLQRGFGQQHIYHF
ECGYAPAGWDTLTKHLLKKGFEFKELEAAGLSKMGKRGPIDQFQRRLLWPIKNLSGDVIGFGARKLFDDDKMGKYMNTPE
TLLYKKSKVLFGLDSAKKAIAAGHQAVVVEGYTDVMAMHAAGIETAVASCGTAFGEEHLQMLRRLMLDDNYFRGELIYTF
DGDEAGQKAAMRAFEGDQKFTGQSFVSVAPNGMDPCDLRLERGDAAVRDLVARRIPMFEFVIQSIISEYTLDTVEGRLAA
LRRAVPIVADIRDKTLQSEYARLLSGWVGWSDPSEVLRQVHEEARRPKRDKKPVRAKRFDQPLEDQSLRPTMALPNPRNP
VLWQERESLKIALQYPELAGSYFDGLPTDSFTNPAYRMVRDAISAAGGCERALDGTDWLPAVSENMTDILGTSLVSELAM
EPIEVEAQDLESYTDGVLSRLQETRVGNQIAILKGQLQRMRPSEDEQAYNSLFSDLVALEQARRELLARAFRG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=437, Percent_Identity=32.7231121281465, Blast_Score=228, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 71056; Mature: 71056

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMAKGRIPESDIQAIRERTPIEEIVGDYVQLKSAGADSLKGLSPFKDEKTPSFHVRPNR
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCEEECCCC
GYYHCFSTGKGGDVFSFLMEMEHISFPEAVEVCAEKIGYQINYQGGGPGRREEPGTRQRL
CEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHH
ILANKAAHQFYREQLETPEAQPGREFLLQRGFGQQHIYHFECGYAPAGWDTLTKHLLKKG
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
FEFKELEAAGLSKMGKRGPIDQFQRRLLWPIKNLSGDVIGFGARKLFDDDKMGKYMNTPE
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHCCCHHCCCCCCCCH
TLLYKKSKVLFGLDSAKKAIAAGHQAVVVEGYTDVMAMHAAGIETAVASCGTAFGEEHLQ
HHHHHHCHHEEECCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MLRRLMLDDNYFRGELIYTFDGDEAGQKAAMRAFEGDQKFTGQSFVSVAPNGMDPCDLRL
HHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHEEE
ERGDAAVRDLVARRIPMFEFVIQSIISEYTLDTVEGRLAALRRAVPIVADIRDKTLQSEY
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ARLLSGWVGWSDPSEVLRQVHEEARRPKRDKKPVRAKRFDQPLEDQSLRPTMALPNPRNP
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCC
VLWQERESLKIALQYPELAGSYFDGLPTDSFTNPAYRMVRDAISAAGGCERALDGTDWLP
CCCCCCCCEEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCH
AVSENMTDILGTSLVSELAMEPIEVEAQDLESYTDGVLSRLQETRVGNQIAILKGQLQRM
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHC
RPSEDEQAYNSLFSDLVALEQARRELLARAFRG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVMAKGRIPESDIQAIRERTPIEEIVGDYVQLKSAGADSLKGLSPFKDEKTPSFHVRPNR
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCEEECCCC
GYYHCFSTGKGGDVFSFLMEMEHISFPEAVEVCAEKIGYQINYQGGGPGRREEPGTRQRL
CEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHH
ILANKAAHQFYREQLETPEAQPGREFLLQRGFGQQHIYHFECGYAPAGWDTLTKHLLKKG
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
FEFKELEAAGLSKMGKRGPIDQFQRRLLWPIKNLSGDVIGFGARKLFDDDKMGKYMNTPE
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHCCCHHCCCCCCCCH
TLLYKKSKVLFGLDSAKKAIAAGHQAVVVEGYTDVMAMHAAGIETAVASCGTAFGEEHLQ
HHHHHHCHHEEECCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MLRRLMLDDNYFRGELIYTFDGDEAGQKAAMRAFEGDQKFTGQSFVSVAPNGMDPCDLRL
HHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHEEE
ERGDAAVRDLVARRIPMFEFVIQSIISEYTLDTVEGRLAALRRAVPIVADIRDKTLQSEY
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ARLLSGWVGWSDPSEVLRQVHEEARRPKRDKKPVRAKRFDQPLEDQSLRPTMALPNPRNP
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCC
VLWQERESLKIALQYPELAGSYFDGLPTDSFTNPAYRMVRDAISAAGGCERALDGTDWLP
CCCCCCCCEEEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCH
AVSENMTDILGTSLVSELAMEPIEVEAQDLESYTDGVLSRLQETRVGNQIAILKGQLQRM
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHC
RPSEDEQAYNSLFSDLVALEQARRELLARAFRG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9422594 [H]