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The map label for this gene is ilvE [H]

Identifier: 145296165

GI number: 145296165

Start: 2299922

End: 2301061

Strand: Reverse

Name: ilvE [H]

Synonym: cgR_2084

Alternate gene names: 145296165

Gene position: 2301061-2299922 (Counterclockwise)

Preceding gene: 145296167

Following gene: 145296164

Centisome position: 69.43

GC content: 52.81

Gene sequence:

>1140_bases
GTGTATCTGTCAGGTAGCAGGTGTACCTTGAAATCCATGACGTCATTAGAGTTCACAGTAACCCGTACCGAAAATCCGAC
GTCACCCGATCGTCTGAAGGAAATTCTTGCCGCACCGAAGTTCGGTAAGTTCTTCACCGACCACATGGTGACCATTGACT
GGAACGAGTTGGAAGGCTGGCACAACGCCCAATTAGTGCCATACGCGCCGATTCCTATGGATCCTGCCACCACCGTATTC
CACTACGGACAGGCAATTTTTGAGGGAATTAAGGCCTACCGCCATTCGGACGAAACCATCAAGACTTTCCGTCCTGATGA
AAACGCCGAACGTATGCAGCGTTCAGCAGCTCGAATGGCAATGCCACAGTTGCCAACCGAGGACTTTATTAAAGCACTTG
AACTGCTGGTAGACGCAGATCAGGATTGGGTTCCTGAGTACGGCGGGGAAGCTTCCCTCTACCTGCGCCCATTCATGATC
TCCACCGAAATTGGCTTGGGTGTCAGCCCAGCTGATGCCTATAAGTTCCTGGTCATCGCATCCCCAGTCGGCGCTTACTT
CACCGGTGGAATCAAGCCTGTTTCCGTCTGGCTGAGCGAAGATTACGTCCGTGCTGCACCCGGCGGAACTGGTGACGCCA
AATTTGCTGGCAACTACGCGGCTTCTTTGCTTGCCCAGTCCCAGGCTGCGGAAAAGGGCTGTGACCAGGTCGTATGGTTG
GATGCCATCGAGCATAAGTACATCGAAGAAATGGGTGGCATGAACCTTGGGTTCATCTACCGCAACGGCGACCACGTCAA
GCTAGTCACCCCTGAACTTTCCGGCTCACTACTTCCAGGCATCACCCGCAAGTCACTTCTACAAGTAGCACGCGACTTGG
GCTACGAAGTAGAAGAGCGAAAGATCACCACCACCGAGTGGGAAGAAGACGCAAAGTCTGGCGCCATGACCGAGGCATTT
GCTTGCGGTACTGCAGCTGTTATCACCCCTGTTGGCACCGTAAAATCAGCTCACGGCACCTTCGAAGTGAACAACAATGA
AGTCGGAGAAATCACGATGAAGCTTCGTGAAACCCTCACCGGAATTCAGCAAGGAAACGTTGAAGACCAAAACGGATGGC
TTTACCCACTGGTTGGCTAA

Upstream 100 bases:

>100_bases
GGGGGAGGCATCAAATTGAAACTAACTTTTAACAAGCCTAGCCATTCCTCAAAACCGTGAGACGAAATTGGCTATTCATC
CCATAAAATGGGGCTGACTA

Downstream 100 bases:

>100_bases
ATCAACCGGTTTTAAGACCCCGCTGCATTAAACCCTGATTTATTGCAGCGGGGTTTTTGCGTTGACCAGCTCTTATGAGA
CGTAGGGGGTGGAGTCAGGG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MYLSGSRCTLKSMTSLEFTVTRTENPTSPDRLKEILAAPKFGKFFTDHMVTIDWNELEGWHNAQLVPYAPIPMDPATTVF
HYGQAIFEGIKAYRHSDETIKTFRPDENAERMQRSAARMAMPQLPTEDFIKALELLVDADQDWVPEYGGEASLYLRPFMI
STEIGLGVSPADAYKFLVIASPVGAYFTGGIKPVSVWLSEDYVRAAPGGTGDAKFAGNYAASLLAQSQAAEKGCDQVVWL
DAIEHKYIEEMGGMNLGFIYRNGDHVKLVTPELSGSLLPGITRKSLLQVARDLGYEVEERKITTTEWEEDAKSGAMTEAF
ACGTAAVITPVGTVKSAHGTFEVNNNEVGEITMKLRETLTGIQQGNVEDQNGWLYPLVG

Sequences:

>Translated_379_residues
MYLSGSRCTLKSMTSLEFTVTRTENPTSPDRLKEILAAPKFGKFFTDHMVTIDWNELEGWHNAQLVPYAPIPMDPATTVF
HYGQAIFEGIKAYRHSDETIKTFRPDENAERMQRSAARMAMPQLPTEDFIKALELLVDADQDWVPEYGGEASLYLRPFMI
STEIGLGVSPADAYKFLVIASPVGAYFTGGIKPVSVWLSEDYVRAAPGGTGDAKFAGNYAASLLAQSQAAEKGCDQVVWL
DAIEHKYIEEMGGMNLGFIYRNGDHVKLVTPELSGSLLPGITRKSLLQVARDLGYEVEERKITTTEWEEDAKSGAMTEAF
ACGTAAVITPVGTVKSAHGTFEVNNNEVGEITMKLRETLTGIQQGNVEDQNGWLYPLVG
>Mature_379_residues
MYLSGSRCTLKSMTSLEFTVTRTENPTSPDRLKEILAAPKFGKFFTDHMVTIDWNELEGWHNAQLVPYAPIPMDPATTVF
HYGQAIFEGIKAYRHSDETIKTFRPDENAERMQRSAARMAMPQLPTEDFIKALELLVDADQDWVPEYGGEASLYLRPFMI
STEIGLGVSPADAYKFLVIASPVGAYFTGGIKPVSVWLSEDYVRAAPGGTGDAKFAGNYAASLLAQSQAAEKGCDQVVWL
DAIEHKYIEEMGGMNLGFIYRNGDHVKLVTPELSGSLLPGITRKSLLQVARDLGYEVEERKITTTEWEEDAKSGAMTEAF
ACGTAAVITPVGTVKSAHGTFEVNNNEVGEITMKLRETLTGIQQGNVEDQNGWLYPLVG

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=332, Percent_Identity=40.0602409638554, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI296010906, Length=332, Percent_Identity=40.0602409638554, Blast_Score=251, Evalue=8e-67,
Organism=Homo sapiens, GI296010904, Length=332, Percent_Identity=40.0602409638554, Blast_Score=251, Evalue=9e-67,
Organism=Homo sapiens, GI50658084, Length=341, Percent_Identity=37.2434017595308, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI258614015, Length=296, Percent_Identity=37.1621621621622, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI296010902, Length=332, Percent_Identity=34.9397590361446, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI296010900, Length=332, Percent_Identity=34.9397590361446, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI48994963, Length=324, Percent_Identity=32.0987654320988, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17568601, Length=362, Percent_Identity=38.121546961326, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI17565728, Length=377, Percent_Identity=33.9522546419098, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6322002, Length=365, Percent_Identity=40.8219178082192, Blast_Score=255, Evalue=7e-69,
Organism=Saccharomyces cerevisiae, GI6322608, Length=372, Percent_Identity=36.8279569892473, Blast_Score=240, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24641779, Length=333, Percent_Identity=40.5405405405405, Blast_Score=234, Evalue=7e-62,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 41727; Mature: 41727

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLSGSRCTLKSMTSLEFTVTRTENPTSPDRLKEILAAPKFGKFFTDHMVTIDWNELEGW
CCCCCCCCCHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCHHHCCEEEEEHHHCCCC
HNAQLVPYAPIPMDPATTVFHYGQAIFEGIKAYRHSDETIKTFRPDENAERMQRSAARMA
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHH
MPQLPTEDFIKALELLVDADQDWVPEYGGEASLYLRPFMISTEIGLGVSPADAYKFLVIA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCHHHEEEEEEE
SPVGAYFTGGIKPVSVWLSEDYVRAAPGGTGDAKFAGNYAASLLAQSQAAEKGCDQVVWL
CCCCHHHCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEH
DAIEHKYIEEMGGMNLGFIYRNGDHVKLVTPELSGSLLPGITRKSLLQVARDLGYEVEER
HHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEECCC
KITTTEWEEDAKSGAMTEAFACGTAAVITPVGTVKSAHGTFEVNNNEVGEITMKLRETLT
EEECCCCHHHHHCCCCHHHHHCCCHHEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
GIQQGNVEDQNGWLYPLVG
HHHCCCCCCCCCEEEEECC
>Mature Secondary Structure
MYLSGSRCTLKSMTSLEFTVTRTENPTSPDRLKEILAAPKFGKFFTDHMVTIDWNELEGW
CCCCCCCCCHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCHHHCCEEEEEHHHCCCC
HNAQLVPYAPIPMDPATTVFHYGQAIFEGIKAYRHSDETIKTFRPDENAERMQRSAARMA
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHH
MPQLPTEDFIKALELLVDADQDWVPEYGGEASLYLRPFMISTEIGLGVSPADAYKFLVIA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCHHHEEEEEEE
SPVGAYFTGGIKPVSVWLSEDYVRAAPGGTGDAKFAGNYAASLLAQSQAAEKGCDQVVWL
CCCCHHHCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEH
DAIEHKYIEEMGGMNLGFIYRNGDHVKLVTPELSGSLLPGITRKSLLQVARDLGYEVEER
HHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCEECCC
KITTTEWEEDAKSGAMTEAFACGTAAVITPVGTVKSAHGTFEVNNNEVGEITMKLRETLT
EEECCCCHHHHHCCCCHHHHHCCCHHEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
GIQQGNVEDQNGWLYPLVG
HHHCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA