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The map label for this gene is murD [H]

Identifier: 145296122

GI number: 145296122

Start: 2249426

End: 2250844

Strand: Reverse

Name: murD [H]

Synonym: cgR_2042

Alternate gene names: 145296122

Gene position: 2250844-2249426 (Counterclockwise)

Preceding gene: 145296123

Following gene: 145296121

Centisome position: 67.92

GC content: 56.1

Gene sequence:

>1419_bases
ATGGTTTCTCTGTCCCATTTACCTCAGGCGCTGCAGGGCCGTATTCTTGTGGCCGGCGCTGGTGTTTCCGGCCTGTCCAT
TGCAAAGATGCTCAGTGAGTTGCATTGCGATGTTGTGGTCGCCGACGATAACGAAACTGCACGTCACATGCTCATTGAAG
TAGTAGACGTTGCAGATATCAGCACCGCGCAGGCTCAGGAACAGCTGGATTCTTTCTCCATTGTGGTCACCTCCCCGGGC
TGGCGCCCAACAAGCACTTTGCTTGTCGACGCCCACCGCCAGGGCCTTGAGGTTATCGGTGACGTCGAGCTTGCTTGGCG
CCTGGACCAGGCAGGTGTTTTCGGCGAGCCACATACGTGGCTCGCAGTTACCGGCACCAACGGTAAAACCACCACCACAT
CAATGCTCGCCGCGATGATGAATGAGGGCGGTTTTGCTGCCAAGGCAGTGGGCAATATCGGCATCCCGGTGTCTGAGGCT
TTGGTAGCGAAAAACCGCATTGATGTGTTAGTTGCAGAGCTGTCTAGTTTCCAATTGCACTGGTCTCCAACCTTCACCCC
TGATGCTGGCGTGGTGCTCAACTTGGCTGAGGATCACATCGATTGGCACGGTTCCATGCGTGATTATGCGTTGGCCAAGA
TGGAAGTGCTCAAGGGCAAGGTCGCCATCATTGGGGCAGACGATCCTTATTTGGTGCAGCTGACTTCTGAAGCAGACTTG
AGTGGTCTCATTGGATTTACCGTCAATGAGCCTGCAACCGGCCAGTTGGGTGTGAAAGCGGGGGAGCTCGTCGATAATGC
CTACGGCAATAATGTGGTTCTTGCATCCGCTGACGGCATTAATCCCGCCGGCCCTGCCGGTGTTTTGGACGCTTTGGCTG
CAGCTGCGGTGGCGCGCTCGCAGGGCGTGGCACCTGAGGCGATCGCGCGTGCGTTGGATTCTTTTGAGGTGGCAGGCCAC
CGTGGCCAGGTCGTCGCCGAGCATGACGGTGTTCATTTCATTGACAACTCCAAGGCGACCAACCCCCACGCTGCTGATTC
TGCGCTAGCTGGGCATGATTCAGTCATTTGGGTTGTCGGCGGACAGCTCAAAGGCGCGGACATTGCGCCACTGGTGAAAA
AGCACGAACAGCGCATCAAGGCAGCATTGGTGTTGGGCGCAGATCGTGCTGAAATCGTGGCAGCGTTGAAGGAACACGCG
CCGCAGGCCTCTGTATTTGTCACTGACAAGACGGAGCCATTTGAGGCAATGGAAGAAATCGTCACTGAGGCATTTAGCAT
CAGCGAACCCGGCGATACCGTGTTGCTTGCCCCTGCCGCTGCGTCTTTGGACATGTTCAAAGGCATGGGCCAGCGTGGCG
ACCTCTTTGCACACAACATCATTGGCACAATCAAAGGATTAACGGAAGAGAAAGGCTGA

Upstream 100 bases:

>100_bases
CGTGGCCATCCGTTTCTGGCTGATCGCGATCATGGCTGTGTTGGCGGGTGTCGGTGTGTTTTACAGCGACTGGCTCCACT
TAGCGGAGGTATAAATAATT

Downstream 100 bases:

>100_bases
TCATGACCACCGGAGCCTCAAAAAAACCCGCACGTCCGAACACTGGCGCTAAAACCAGAACGGGGCTGGGAATTAGGGAG
CGTATTTCCGGTGCATGGAA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MVSLSHLPQALQGRILVAGAGVSGLSIAKMLSELHCDVVVADDNETARHMLIEVVDVADISTAQAQEQLDSFSIVVTSPG
WRPTSTLLVDAHRQGLEVIGDVELAWRLDQAGVFGEPHTWLAVTGTNGKTTTTSMLAAMMNEGGFAAKAVGNIGIPVSEA
LVAKNRIDVLVAELSSFQLHWSPTFTPDAGVVLNLAEDHIDWHGSMRDYALAKMEVLKGKVAIIGADDPYLVQLTSEADL
SGLIGFTVNEPATGQLGVKAGELVDNAYGNNVVLASADGINPAGPAGVLDALAAAAVARSQGVAPEAIARALDSFEVAGH
RGQVVAEHDGVHFIDNSKATNPHAADSALAGHDSVIWVVGGQLKGADIAPLVKKHEQRIKAALVLGADRAEIVAALKEHA
PQASVFVTDKTEPFEAMEEIVTEAFSISEPGDTVLLAPAAASLDMFKGMGQRGDLFAHNIIGTIKGLTEEKG

Sequences:

>Translated_472_residues
MVSLSHLPQALQGRILVAGAGVSGLSIAKMLSELHCDVVVADDNETARHMLIEVVDVADISTAQAQEQLDSFSIVVTSPG
WRPTSTLLVDAHRQGLEVIGDVELAWRLDQAGVFGEPHTWLAVTGTNGKTTTTSMLAAMMNEGGFAAKAVGNIGIPVSEA
LVAKNRIDVLVAELSSFQLHWSPTFTPDAGVVLNLAEDHIDWHGSMRDYALAKMEVLKGKVAIIGADDPYLVQLTSEADL
SGLIGFTVNEPATGQLGVKAGELVDNAYGNNVVLASADGINPAGPAGVLDALAAAAVARSQGVAPEAIARALDSFEVAGH
RGQVVAEHDGVHFIDNSKATNPHAADSALAGHDSVIWVVGGQLKGADIAPLVKKHEQRIKAALVLGADRAEIVAALKEHA
PQASVFVTDKTEPFEAMEEIVTEAFSISEPGDTVLLAPAAASLDMFKGMGQRGDLFAHNIIGTIKGLTEEKG
>Mature_472_residues
MVSLSHLPQALQGRILVAGAGVSGLSIAKMLSELHCDVVVADDNETARHMLIEVVDVADISTAQAQEQLDSFSIVVTSPG
WRPTSTLLVDAHRQGLEVIGDVELAWRLDQAGVFGEPHTWLAVTGTNGKTTTTSMLAAMMNEGGFAAKAVGNIGIPVSEA
LVAKNRIDVLVAELSSFQLHWSPTFTPDAGVVLNLAEDHIDWHGSMRDYALAKMEVLKGKVAIIGADDPYLVQLTSEADL
SGLIGFTVNEPATGQLGVKAGELVDNAYGNNVVLASADGINPAGPAGVLDALAAAAVARSQGVAPEAIARALDSFEVAGH
RGQVVAEHDGVHFIDNSKATNPHAADSALAGHDSVIWVVGGQLKGADIAPLVKKHEQRIKAALVLGADRAEIVAALKEHA
PQASVFVTDKTEPFEAMEEIVTEAFSISEPGDTVLLAPAAASLDMFKGMGQRGDLFAHNIIGTIKGLTEEKG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=455, Percent_Identity=30.7692307692308, Blast_Score=149, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49283; Mature: 49283

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLSHLPQALQGRILVAGAGVSGLSIAKMLSELHCDVVVADDNETARHMLIEVVDVADI
CCCHHHCHHHHCCEEEEEECCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCC
STAQAQEQLDSFSIVVTSPGWRPTSTLLVDAHRQGLEVIGDVELAWRLDQAGVFGEPHTW
CHHHHHHHHCCEEEEEECCCCCCCCEEEEECCCCCCCEEECEEEEEEECCCCCCCCCCEE
LAVTGTNGKTTTTSMLAAMMNEGGFAAKAVGNIGIPVSEALVAKNRIDVLVAELSSFQLH
EEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCHHHHEEEEHHCEEEE
WSPTFTPDAGVVLNLAEDHIDWHGSMRDYALAKMEVLKGKVAIIGADDPYLVQLTSEADL
ECCCCCCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCC
SGLIGFTVNEPATGQLGVKAGELVDNAYGNNVVLASADGINPAGPAGVLDALAAAAVARS
CEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
QGVAPEAIARALDSFEVAGHRGQVVAEHDGVHFIDNSKATNPHAADSALAGHDSVIWVVG
CCCCHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEC
GQLKGADIAPLVKKHEQRIKAALVLGADRAEIVAALKEHAPQASVFVTDKTEPFEAMEEI
CCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHH
VTEAFSISEPGDTVLLAPAAASLDMFKGMGQRGDLFAHNIIGTIKGLTEEKG
HHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVSLSHLPQALQGRILVAGAGVSGLSIAKMLSELHCDVVVADDNETARHMLIEVVDVADI
CCCHHHCHHHHCCEEEEEECCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCC
STAQAQEQLDSFSIVVTSPGWRPTSTLLVDAHRQGLEVIGDVELAWRLDQAGVFGEPHTW
CHHHHHHHHCCEEEEEECCCCCCCCEEEEECCCCCCCEEECEEEEEEECCCCCCCCCCEE
LAVTGTNGKTTTTSMLAAMMNEGGFAAKAVGNIGIPVSEALVAKNRIDVLVAELSSFQLH
EEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCHHHHEEEEHHCEEEE
WSPTFTPDAGVVLNLAEDHIDWHGSMRDYALAKMEVLKGKVAIIGADDPYLVQLTSEADL
ECCCCCCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCC
SGLIGFTVNEPATGQLGVKAGELVDNAYGNNVVLASADGINPAGPAGVLDALAAAAVARS
CEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
QGVAPEAIARALDSFEVAGHRGQVVAEHDGVHFIDNSKATNPHAADSALAGHDSVIWVVG
CCCCHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEC
GQLKGADIAPLVKKHEQRIKAALVLGADRAEIVAALKEHAPQASVFVTDKTEPFEAMEEI
CCCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHH
VTEAFSISEPGDTVLLAPAAASLDMFKGMGQRGDLFAHNIIGTIKGLTEEKG
HHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]