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The map label for this gene is purR [H]

Identifier: 145296073

GI number: 145296073

Start: 2193591

End: 2194589

Strand: Direct

Name: purR [H]

Synonym: cgR_1995

Alternate gene names: 145296073

Gene position: 2193591-2194589 (Clockwise)

Preceding gene: 145296070

Following gene: 145296078

Centisome position: 66.19

GC content: 55.96

Gene sequence:

>999_bases
ATGGCGTCAAAGCGACCGACAATGGCTGATGTGGCAAAAGCTGCTGGAGTATCCACTGCGCTGGTCTCCATCGTGTTTCG
CGATGCCCCCGGAGCAAGTGAATCCACCCGCAACCATGTGAAAGAAAAAGCCGCCGAACTCGGATACATTCCTGATCGAC
GAGCCCAAAAACTTCGCCAAAACCGCTCCGGACTCATCGGTGTGGCATTCGAAATGCACCAAGCATTCCACGGCGATATC
GTCGAACACCTCTATCCCACCGCCCGAAAACATGGCTTCGACCTGCACCTTAGCGCGATCACACCGACTCGCACTGAAAA
AGATGCCGTCAACGCCCTGATCAGGGAACGATGCGAAGCAGTAATTCTTCTAGGATCTCGCATGTCACCTAGTGATTTGG
AAACAATCGCACAGCAACTTCCCGTCCAAGTAATTGCCCGCGGTTCCGGAACCCCCAAAGTCAGTTCCGTCCATGTCGAC
GACGCAGTTGGCGCCCAATTAGCCCTCAACCACCTCATCGAATTAGGCCACGAACACATCATCTACATCGATGGCGGCGA
CGCCCCTGGCACCCAGGAACGATCAGAAGTGTTCAAAGCGCACATTGATTCCTTCGCCGGCACCACCCGCATAGTCCCTG
GTGGCAGCAGCGAAGCAGCAGGAGCCCAAGCAATCTCAGAGATTCTGGATTCCGGAACTGACGCCACCGCCATCCTCGCA
TTCAATGACCGAGTAGCCCTCGGCGTGCTCGACATCTTATGGCAGCGCGGCATCCACGTCCCCGAAGAAATCTCCGTGGT
GGGATACGACAACTCGAGCCTTGCCAGCCTCAACCACATCGGCTTGACCACCATCGCGCAGGACTCCGAAGCAATCGCGG
TGAAAGCCATCGATGTCACGATCAGTCAGATCAACAATCACACCGAAACTTCTGCGGTGCTGACCCCAACATTGATCACA
CGGCGCACCACGGGTCCTCGGCCACAGAAGGCGATTTAA

Upstream 100 bases:

>100_bases
ACGTTCTACTATAACGTTCTAGTCGATACTAGATACCACCCATTGATGCCGTCAAGGGGTTTCCTGTAAAGATGTAAGAG
ATTAAGAAAAAGAGGTAGAT

Downstream 100 bases:

>100_bases
GAGGTCGAAAATACCGAAGGTGCACACCGTCTGGAGTTGCCAGTTCCAATCCTTTTGACGCGCATGGAGGCCTATGTATT
TTTAGCGCGATTGGAGCTGG

Product: hypothetical protein

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MASKRPTMADVAKAAGVSTALVSIVFRDAPGASESTRNHVKEKAAELGYIPDRRAQKLRQNRSGLIGVAFEMHQAFHGDI
VEHLYPTARKHGFDLHLSAITPTRTEKDAVNALIRERCEAVILLGSRMSPSDLETIAQQLPVQVIARGSGTPKVSSVHVD
DAVGAQLALNHLIELGHEHIIYIDGGDAPGTQERSEVFKAHIDSFAGTTRIVPGGSSEAAGAQAISEILDSGTDATAILA
FNDRVALGVLDILWQRGIHVPEEISVVGYDNSSLASLNHIGLTTIAQDSEAIAVKAIDVTISQINNHTETSAVLTPTLIT
RRTTGPRPQKAI

Sequences:

>Translated_332_residues
MASKRPTMADVAKAAGVSTALVSIVFRDAPGASESTRNHVKEKAAELGYIPDRRAQKLRQNRSGLIGVAFEMHQAFHGDI
VEHLYPTARKHGFDLHLSAITPTRTEKDAVNALIRERCEAVILLGSRMSPSDLETIAQQLPVQVIARGSGTPKVSSVHVD
DAVGAQLALNHLIELGHEHIIYIDGGDAPGTQERSEVFKAHIDSFAGTTRIVPGGSSEAAGAQAISEILDSGTDATAILA
FNDRVALGVLDILWQRGIHVPEEISVVGYDNSSLASLNHIGLTTIAQDSEAIAVKAIDVTISQINNHTETSAVLTPTLIT
RRTTGPRPQKAI
>Mature_331_residues
ASKRPTMADVAKAAGVSTALVSIVFRDAPGASESTRNHVKEKAAELGYIPDRRAQKLRQNRSGLIGVAFEMHQAFHGDIV
EHLYPTARKHGFDLHLSAITPTRTEKDAVNALIRERCEAVILLGSRMSPSDLETIAQQLPVQVIARGSGTPKVSSVHVDD
AVGAQLALNHLIELGHEHIIYIDGGDAPGTQERSEVFKAHIDSFAGTTRIVPGGSSEAAGAQAISEILDSGTDATAILAF
NDRVALGVLDILWQRGIHVPEEISVVGYDNSSLASLNHIGLTTIAQDSEAIAVKAIDVTISQINNHTETSAVLTPTLITR
RTTGPRPQKAI

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=328, Percent_Identity=31.0975609756098, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=30.1204819277108, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1788474, Length=337, Percent_Identity=29.3768545994065, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786540, Length=346, Percent_Identity=31.2138728323699, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1789068, Length=292, Percent_Identity=28.4246575342466, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=28.1065088757396, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=29.5180722891566, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787906, Length=327, Percent_Identity=25.3822629969419, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787580, Length=287, Percent_Identity=25.7839721254355, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=22.6708074534161, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789456, Length=309, Percent_Identity=24.2718446601942, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790715, Length=278, Percent_Identity=22.3021582733813, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35394; Mature: 35263

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKRPTMADVAKAAGVSTALVSIVFRDAPGASESTRNHVKEKAAELGYIPDRRAQKLRQ
CCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
NRSGLIGVAFEMHQAFHGDIVEHLYPTARKHGFDLHLSAITPTRTEKDAVNALIRERCEA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHE
VILLGSRMSPSDLETIAQQLPVQVIARGSGTPKVSSVHVDDAVGAQLALNHLIELGHEHI
EEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEE
IYIDGGDAPGTQERSEVFKAHIDSFAGTTRIVPGGSSEAAGAQAISEILDSGTDATAILA
EEEECCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEE
FNDRVALGVLDILWQRGIHVPEEISVVGYDNSSLASLNHIGLTTIAQDSEAIAVKAIDVT
ECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCCEEEECCCCEEEEEEEEEE
ISQINNHTETSAVLTPTLITRRTTGPRPQKAI
HHHHCCCCCCHHEECHHHHEECCCCCCCCCCC
>Mature Secondary Structure 
ASKRPTMADVAKAAGVSTALVSIVFRDAPGASESTRNHVKEKAAELGYIPDRRAQKLRQ
CCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
NRSGLIGVAFEMHQAFHGDIVEHLYPTARKHGFDLHLSAITPTRTEKDAVNALIRERCEA
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHE
VILLGSRMSPSDLETIAQQLPVQVIARGSGTPKVSSVHVDDAVGAQLALNHLIELGHEHI
EEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEE
IYIDGGDAPGTQERSEVFKAHIDSFAGTTRIVPGGSSEAAGAQAISEILDSGTDATAILA
EEEECCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEE
FNDRVALGVLDILWQRGIHVPEEISVVGYDNSSLASLNHIGLTTIAQDSEAIAVKAIDVT
ECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCCCEEEECCCCEEEEEEEEEE
ISQINNHTETSAVLTPTLITRRTTGPRPQKAI
HHHHCCCCCCHHEECHHHHEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA