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The map label for this gene is glgX [H]

Identifier: 145296069

GI number: 145296069

Start: 2188213

End: 2190723

Strand: Direct

Name: glgX [H]

Synonym: cgR_1991

Alternate gene names: 145296069

Gene position: 2188213-2190723 (Clockwise)

Preceding gene: 145296068

Following gene: 145296070

Centisome position: 66.03

GC content: 54.28

Gene sequence:

>2511_bases
ATGACATCAACGACTGAGCACTCGTACCAAGTTTGGCCTGGACATGCTTATCCTTTGGGTTCAACCTATGACGGTGCTGG
AACGAACTTCGCAATCTTCTCCGACGTTGCAGAGCGTGTCGAGCTGTGTCTATTAGATGCAGATAATAACGAGACTCGAA
TTCCGCTCGAAGAGCGTGATGCCCACATTTGGCATTGCTACCTTCCTGGGGTTCAACCTGGACAGCGCTACGGATTCCGA
GTTCATGGTCCGTGGAACCCAGATGAGGGTAAGCGGTGCGACGCGAACAAACTTCTAGTTGATCCATATGCTCGTGCTTT
CGATGGAGATTTTGATGGACATCCGTCACTATTTTCTTACGACATCACCAATCCAAATGACCCCAACGGTCGCAATACCG
AAGACAGCATTGACCACACCATGAAGTCTGTCGTGGTGAACCCATTCTTCGACTGGGGAAACGATCGTGCACCGCGCATT
CCTTACAATGAAACAGTCATTTACGAAGCCCACGTCAAGGGCATGACGATGACACACCCGGAGGTTCCTGAAGAACTGCG
TGGCACCTACGCGGGCCTTGCCCACCCAGCCATCATTCAATACTTGAGTGATCTGGGAGTGACTGCCATTGAATTGATGC
CGGTCCACCAGTTCCTGCAGGATGATCGTCTCCGCGACCTAGGAATGCGCAACTACTGGGGCTACAACTCTTTCGGCTTC
TTTGCGCCCTACAACGATTACGCTGCCAACAAGAACCCCGGTGGCGCCGTGGCCGAGTTCAAAGGCTTGGTGCGCAGCTA
CCACGAAGCGGGCCTCGAAGTAATTTTGGATGTGGTCTACAACCACACCGCCGAGGGCAACCACATGGGCCCCACCATCG
CGTTCCGAGGCATTGACAACGAGGCGTACTACCGACTGGTTGAGGGCGATCGCCGTCATTACATGGACTACACCGGTACT
GGTAACTCCCTCAATGTCCGCGACCCGCACTCACTGCAGCTGATTATGGATTCGCTGCGCTACTGGGTCACCGAGATGCA
TGTCGACGGCTTCCGCTTCGACCTTGCCTCTACCCTTGCTCGTGAATTTGATGATGTTGACCGCCTGGCAACCTTCTTCG
ACCTGGTCCAACAAGACCCGGTGGTCTCCCAGGTCAAGCTCATTGCTGAGCCGTGGGATGTCGGCGAAGGCGGATACCAA
GTGGGTAACTTCCCACCACTGTGGACTGAGTGGAATGGTAAATACCGCGACACTGTCCGTGATTTCTGGCGTGGTGAGCC
AGCAACCTTGGGTGAATTCGCTTCCCGACTAACTGGTTCCTCTGATTTGTATGCAAACAACGGCCGTCGCCCCACTGCAT
CGATCAACTTTGTGACTGCTCACGACGGCTTCACCCTCAATGACTTGGTCAGTTACAACGAGAAGCACAACATGGCCAAC
GGTGAAGACGGTCGGGACGGTGAATCACACAACCGTTCCTGGAACTGTGGCGTCGAAGGACCAACTGACGATCCTGAGAT
TATGCAGCTGCGTGCTCAGCAACGACGCAACTTCCTCACCACCTTGTTGCTGTCCCAGGGCACCCCTATGTTGTCCCACG
GTGATGAAATGGCCCGTACCCAAAACGGCAACAACAACGTCTACTGCCAAGACAATGAATTGGCGTGGGTGAATTGGGAT
CAGGCTGAAGAAAACGCTGACTTGGTGAGCTTCACCAGGCGTTTGCTGCGTATCCGAGCAAACCACCCAGTATTTAGGCG
CAGGCAGTTCCTTGCCGGTGGCCCTTTGGGCGCCGATGTTCGTGACCGCGATATCGCATGGCTGGTACCAAATGGAACCT
TGATGACTCAAGATGACTGGGACTTCGCTTTCGGTAAATCACTGCAGGTGTTCTTCAACGGCGATGCCATCGAAGAGCCT
GATTATCGAGGACAGAAAATCCACGATGACTCCTTCATCTTGATGTTCAACGCTCACTTCGAACCTATCGATTTCAATCT
CCCTCCTGAGCATTTCGGTATGAAGTGGAAGCTTTTGGTCGATACCACCGAAGCGGTGGGCCACCCGCTGGAGGATCTCA
CCATCGAAGCTGGCGGAACCATCACTGTTCCTGCCCGTTCCACGATGCTGCTGCGCCAGGTGGAGGCTCCGGACTACACC
AAGCTTGAGGAAAAGATCGCTGCTGAAAAGCGTGAGCAAGAACTTGCGGCAGAGAAGGAAGCTGCTGAGAAGCGCGAATT
GGAACTGGCGGCAGCAAAGGAAGCTGAAGATGCTGCTGAGGCTCTCCGCCTTGCGGCAGAACGTGCTTCGACTCAGGAAG
CTGAATTGGCCCATCAACACGGTGCTGATGCGATTGCCGATGAGGTAGCGGAAGAACCACAAGAGCTGCCACAAGATGAA
GTAGCGGCAGAGGTCGAGACTGAGCCTGACACCGAGCCTGACACTGAATCTGACTCCGAGCAGGCTGAGGTAGCTTCAGA
GGAGCCTGAAGCGGACGAAGAAGAGAAGTAG

Upstream 100 bases:

>100_bases
AGAAAACCCTGCCGATGCGAACTTTGAGGAGAGATTCAATCAAGGATTAGAAATCATTCTGGTGGGTCTAGACGCGCTTG
GGCATATAAGATAGCGTTCT

Downstream 100 bases:

>100_bases
TACACCGAAAGTGGCGTCGCAGGGATGTTCCTGCGGCACCATTTTTGTTGAGGTGGAACTCACGGATTAAACACGGATTT
TTCTAAGGTTAATCAAGTAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 836; Mature: 835

Protein sequence:

>836_residues
MTSTTEHSYQVWPGHAYPLGSTYDGAGTNFAIFSDVAERVELCLLDADNNETRIPLEERDAHIWHCYLPGVQPGQRYGFR
VHGPWNPDEGKRCDANKLLVDPYARAFDGDFDGHPSLFSYDITNPNDPNGRNTEDSIDHTMKSVVVNPFFDWGNDRAPRI
PYNETVIYEAHVKGMTMTHPEVPEELRGTYAGLAHPAIIQYLSDLGVTAIELMPVHQFLQDDRLRDLGMRNYWGYNSFGF
FAPYNDYAANKNPGGAVAEFKGLVRSYHEAGLEVILDVVYNHTAEGNHMGPTIAFRGIDNEAYYRLVEGDRRHYMDYTGT
GNSLNVRDPHSLQLIMDSLRYWVTEMHVDGFRFDLASTLAREFDDVDRLATFFDLVQQDPVVSQVKLIAEPWDVGEGGYQ
VGNFPPLWTEWNGKYRDTVRDFWRGEPATLGEFASRLTGSSDLYANNGRRPTASINFVTAHDGFTLNDLVSYNEKHNMAN
GEDGRDGESHNRSWNCGVEGPTDDPEIMQLRAQQRRNFLTTLLLSQGTPMLSHGDEMARTQNGNNNVYCQDNELAWVNWD
QAEENADLVSFTRRLLRIRANHPVFRRRQFLAGGPLGADVRDRDIAWLVPNGTLMTQDDWDFAFGKSLQVFFNGDAIEEP
DYRGQKIHDDSFILMFNAHFEPIDFNLPPEHFGMKWKLLVDTTEAVGHPLEDLTIEAGGTITVPARSTMLLRQVEAPDYT
KLEEKIAAEKREQELAAEKEAAEKRELELAAAKEAEDAAEALRLAAERASTQEAELAHQHGADAIADEVAEEPQELPQDE
VAAEVETEPDTEPDTESDSEQAEVASEEPEADEEEK

Sequences:

>Translated_836_residues
MTSTTEHSYQVWPGHAYPLGSTYDGAGTNFAIFSDVAERVELCLLDADNNETRIPLEERDAHIWHCYLPGVQPGQRYGFR
VHGPWNPDEGKRCDANKLLVDPYARAFDGDFDGHPSLFSYDITNPNDPNGRNTEDSIDHTMKSVVVNPFFDWGNDRAPRI
PYNETVIYEAHVKGMTMTHPEVPEELRGTYAGLAHPAIIQYLSDLGVTAIELMPVHQFLQDDRLRDLGMRNYWGYNSFGF
FAPYNDYAANKNPGGAVAEFKGLVRSYHEAGLEVILDVVYNHTAEGNHMGPTIAFRGIDNEAYYRLVEGDRRHYMDYTGT
GNSLNVRDPHSLQLIMDSLRYWVTEMHVDGFRFDLASTLAREFDDVDRLATFFDLVQQDPVVSQVKLIAEPWDVGEGGYQ
VGNFPPLWTEWNGKYRDTVRDFWRGEPATLGEFASRLTGSSDLYANNGRRPTASINFVTAHDGFTLNDLVSYNEKHNMAN
GEDGRDGESHNRSWNCGVEGPTDDPEIMQLRAQQRRNFLTTLLLSQGTPMLSHGDEMARTQNGNNNVYCQDNELAWVNWD
QAEENADLVSFTRRLLRIRANHPVFRRRQFLAGGPLGADVRDRDIAWLVPNGTLMTQDDWDFAFGKSLQVFFNGDAIEEP
DYRGQKIHDDSFILMFNAHFEPIDFNLPPEHFGMKWKLLVDTTEAVGHPLEDLTIEAGGTITVPARSTMLLRQVEAPDYT
KLEEKIAAEKREQELAAEKEAAEKRELELAAAKEAEDAAEALRLAAERASTQEAELAHQHGADAIADEVAEEPQELPQDE
VAAEVETEPDTEPDTESDSEQAEVASEEPEADEEEK
>Mature_835_residues
TSTTEHSYQVWPGHAYPLGSTYDGAGTNFAIFSDVAERVELCLLDADNNETRIPLEERDAHIWHCYLPGVQPGQRYGFRV
HGPWNPDEGKRCDANKLLVDPYARAFDGDFDGHPSLFSYDITNPNDPNGRNTEDSIDHTMKSVVVNPFFDWGNDRAPRIP
YNETVIYEAHVKGMTMTHPEVPEELRGTYAGLAHPAIIQYLSDLGVTAIELMPVHQFLQDDRLRDLGMRNYWGYNSFGFF
APYNDYAANKNPGGAVAEFKGLVRSYHEAGLEVILDVVYNHTAEGNHMGPTIAFRGIDNEAYYRLVEGDRRHYMDYTGTG
NSLNVRDPHSLQLIMDSLRYWVTEMHVDGFRFDLASTLAREFDDVDRLATFFDLVQQDPVVSQVKLIAEPWDVGEGGYQV
GNFPPLWTEWNGKYRDTVRDFWRGEPATLGEFASRLTGSSDLYANNGRRPTASINFVTAHDGFTLNDLVSYNEKHNMANG
EDGRDGESHNRSWNCGVEGPTDDPEIMQLRAQQRRNFLTTLLLSQGTPMLSHGDEMARTQNGNNNVYCQDNELAWVNWDQ
AEENADLVSFTRRLLRIRANHPVFRRRQFLAGGPLGADVRDRDIAWLVPNGTLMTQDDWDFAFGKSLQVFFNGDAIEEPD
YRGQKIHDDSFILMFNAHFEPIDFNLPPEHFGMKWKLLVDTTEAVGHPLEDLTIEAGGTITVPARSTMLLRQVEAPDYTK
LEEKIAAEKREQELAAEKEAAEKRELELAAAKEAEDAAEALRLAAERASTQEAELAHQHGADAIADEVAEEPQELPQDEV
AAEVETEPDTEPDTESDSEQAEVASEEPEADEEEK

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=660, Percent_Identity=46.2121212121212, Blast_Score=571, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 94326; Mature: 94194

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTTEHSYQVWPGHAYPLGSTYDGAGTNFAIFSDVAERVELCLLDADNNETRIPLEERD
CCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCCCHHHCC
AHIWHCYLPGVQPGQRYGFRVHGPWNPDEGKRCDANKLLVDPYARAFDGDFDGHPSLFSY
CEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCCCEEEE
DITNPNDPNGRNTEDSIDHTMKSVVVNPFFDWGNDRAPRIPYNETVIYEAHVKGMTMTHP
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCEECCCC
EVPEELRGTYAGLAHPAIIQYLSDLGVTAIELMPVHQFLQDDRLRDLGMRNYWGYNSFGF
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
FAPYNDYAANKNPGGAVAEFKGLVRSYHEAGLEVILDVVYNHTAEGNHMGPTIAFRGIDN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
EAYYRLVEGDRRHYMDYTGTGNSLNVRDPHSLQLIMDSLRYWVTEMHVDGFRFDLASTLA
HHEEEEECCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
REFDDVDRLATFFDLVQQDPVVSQVKLIAEPWDVGEGGYQVGNFPPLWTEWNGKYRDTVR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHH
DFWRGEPATLGEFASRLTGSSDLYANNGRRPTASINFVTAHDGFTLNDLVSYNEKHNMAN
HHHCCCCCCHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCC
GEDGRDGESHNRSWNCGVEGPTDDPEIMQLRAQQRRNFLTTLLLSQGTPMLSHGDEMART
CCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHH
QNGNNNVYCQDNELAWVNWDQAEENADLVSFTRRLLRIRANHPVFRRRQFLAGGPLGADV
CCCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
RDRDIAWLVPNGTLMTQDDWDFAFGKSLQVFFNGDAIEEPDYRGQKIHDDSFILMFNAHF
CCCCEEEECCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCEEEEEECCC
EPIDFNLPPEHFGMKWKLLVDTTEAVGHPLEDLTIEAGGTITVPARSTMLLRQVEAPDYT
CEECCCCCHHHHCCEEEEEEECHHHHCCCHHHEEEECCCEEEECCCCEEHHHHCCCCCHH
KLEEKIAAEKREQELAAEKEAAEKRELELAAAKEAEDAAEALRLAAERASTQEAELAHQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
GADAIADEVAEEPQELPQDEVAAEVETEPDTEPDTESDSEQAEVASEEPEADEEEK
CCHHHHHHHHHHHHHCCHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TSTTEHSYQVWPGHAYPLGSTYDGAGTNFAIFSDVAERVELCLLDADNNETRIPLEERD
CCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCCCHHHCC
AHIWHCYLPGVQPGQRYGFRVHGPWNPDEGKRCDANKLLVDPYARAFDGDFDGHPSLFSY
CEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCCCEEEE
DITNPNDPNGRNTEDSIDHTMKSVVVNPFFDWGNDRAPRIPYNETVIYEAHVKGMTMTHP
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCEECCCC
EVPEELRGTYAGLAHPAIIQYLSDLGVTAIELMPVHQFLQDDRLRDLGMRNYWGYNSFGF
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
FAPYNDYAANKNPGGAVAEFKGLVRSYHEAGLEVILDVVYNHTAEGNHMGPTIAFRGIDN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
EAYYRLVEGDRRHYMDYTGTGNSLNVRDPHSLQLIMDSLRYWVTEMHVDGFRFDLASTLA
HHEEEEECCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
REFDDVDRLATFFDLVQQDPVVSQVKLIAEPWDVGEGGYQVGNFPPLWTEWNGKYRDTVR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHH
DFWRGEPATLGEFASRLTGSSDLYANNGRRPTASINFVTAHDGFTLNDLVSYNEKHNMAN
HHHCCCCCCHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCC
GEDGRDGESHNRSWNCGVEGPTDDPEIMQLRAQQRRNFLTTLLLSQGTPMLSHGDEMART
CCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHH
QNGNNNVYCQDNELAWVNWDQAEENADLVSFTRRLLRIRANHPVFRRRQFLAGGPLGADV
CCCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
RDRDIAWLVPNGTLMTQDDWDFAFGKSLQVFFNGDAIEEPDYRGQKIHDDSFILMFNAHF
CCCCEEEECCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCEEEEEECCC
EPIDFNLPPEHFGMKWKLLVDTTEAVGHPLEDLTIEAGGTITVPARSTMLLRQVEAPDYT
CEECCCCCHHHHCCEEEEEEECHHHHCCCHHHEEEECCCEEEECCCCEEHHHHCCCCCHH
KLEEKIAAEKREQELAAEKEAAEKRELELAAAKEAEDAAEALRLAAERASTQEAELAHQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
GADAIADEVAEEPQELPQDEVAAEVETEPDTEPDTESDSEQAEVASEEPEADEEEK
CCHHHHHHHHHHHHHCCHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]