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The map label for this gene is amt [H]

Identifier: 145295704

GI number: 145295704

Start: 1813477

End: 1814835

Strand: Reverse

Name: amt [H]

Synonym: cgR_1631

Alternate gene names: 145295704

Gene position: 1814835-1813477 (Counterclockwise)

Preceding gene: 145295705

Following gene: 145295703

Centisome position: 54.76

GC content: 55.56

Gene sequence:

>1359_bases
ATGGACCCCTCAGATCTAGCCTGGATTCTCGCAGCCTTTGCATTGGTAAGCCTGATGTTTCCCGGATTGTCCCTGCTTTA
CGGCGGCATGTTGGGTGGGCAACACGTTCTTAATACGTTCATGATGGTTATGAGCTCACTTGGAATCATCAGCCTGGTGT
ACATCTTTTACGGACACGGCCTGGTTTTAGGAAACTCCATCGGTGGGTGGGGGATTATCGGAAATCCACTTGAATACTTC
GGCTTCCGCAACATCATGGAAGATGACGGCACCGGAGACCTCATGTGGGCCGGCTTCTACATTCTGTTCGCTGCAATCTC
ACTCGCACTTGTTTCATCTGGTGCAGCAGGGCGCATGCGCTTTGGAGCGTGGCTGGTCTTCGGTGTCCTGTGGTTCACCT
TTGTGTACGCGCCACTGGCACACTGGGTTTTCGCTATCGATGATCCTGAGTCCGGCTACGTGGGTGGCTGGATGAAAAAT
GTGCTTGAGTTCCACGACTTTGCTGGTGGAACGGCAGTGCACATGAATGCGGGTGCTTCTGGACTTGCACTGGCAATGGT
GCTGGGTCGTCGCCACTCCATGGCGGTGCGTCCACACAATCTGCCCCTGATTTTGATTGGTGCAGGACTGATCATTGCGG
GCTGGTTCGGATTCAATGGTGGTACCGCAGGTGGTGCCAACTTCCTCGCAAGCTACGTAGTTGTTACCTCTCTCATTGCT
GCAGCCGGCGGAATGATGGGCTTCATGCTCGTTGAACGAGTGTTCAGCGGAAAGCCCACCTTCTTTGGCTCGGCAACCGG
CACAATCGCAGGCCTTGTGGCTATCACCCCGGCCGCGGATGCAGTGAGCCCGCTCGGAGCATTCGCCGTCGGAGCGCTCG
GCGCAGTTGTCTCCTTCTGGGCAATTAGCTGGAAGAAGGGACACCGAGTCGATGATTCCTTCGATGTGTTCGCAGTCCAC
GGAATGGCCGGCATTGCAGGTGCACTGTTCGTCATGCTCTTTGGCGATCCACTAGCACCAGCGGGAGTTTCCGGAGTCTT
CTTCGGTGGCGAACTCTCCCTGCTGTGGAGGGAACCACTGGCCATCATCGTGACCCTTGCATACGCATTCGGCGTCACCT
GGTTGATTGCCACGATCTTGAACAAGTTCATGACTCTGCGCATCACCTCCGAAGCCGAATATGAAGGCATCGACCGCGCA
GAACACGCGGAATCTGCCTACCACCTCAATTCCAACGGAATTGGGATGGCAACCCGCACCAATTTCGGACCTGAAATCCC
CGAGGAAACCGTGCCCGACGCCGTGCAGGTGGGCGTCGATAAGCAAAAAATCGCTGATACTCGAAAGGCCTCAAAATGA

Upstream 100 bases:

>100_bases
GAAATCTATAGAACGATAGGTAGAAACTGGACTAGATTTATCTATAGCGGAATAGAAAATACTCCGCTCGATAGCATCAC
TTAGCTGAAAGGCCTTTAAA

Downstream 100 bases:

>100_bases
CCGCAACCTACACCACTGAAACCGCTATCAATTTCTTGTTCTTGAGCGAACCGGACATGATCGCGGCCGGAGTCAAAGAC
GTCGCGCAATGCGTCGATGT

Product: hypothetical protein

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MDPSDLAWILAAFALVSLMFPGLSLLYGGMLGGQHVLNTFMMVMSSLGIISLVYIFYGHGLVLGNSIGGWGIIGNPLEYF
GFRNIMEDDGTGDLMWAGFYILFAAISLALVSSGAAGRMRFGAWLVFGVLWFTFVYAPLAHWVFAIDDPESGYVGGWMKN
VLEFHDFAGGTAVHMNAGASGLALAMVLGRRHSMAVRPHNLPLILIGAGLIIAGWFGFNGGTAGGANFLASYVVVTSLIA
AAGGMMGFMLVERVFSGKPTFFGSATGTIAGLVAITPAADAVSPLGAFAVGALGAVVSFWAISWKKGHRVDDSFDVFAVH
GMAGIAGALFVMLFGDPLAPAGVSGVFFGGELSLLWREPLAIIVTLAYAFGVTWLIATILNKFMTLRITSEAEYEGIDRA
EHAESAYHLNSNGIGMATRTNFGPEIPEETVPDAVQVGVDKQKIADTRKASK

Sequences:

>Translated_452_residues
MDPSDLAWILAAFALVSLMFPGLSLLYGGMLGGQHVLNTFMMVMSSLGIISLVYIFYGHGLVLGNSIGGWGIIGNPLEYF
GFRNIMEDDGTGDLMWAGFYILFAAISLALVSSGAAGRMRFGAWLVFGVLWFTFVYAPLAHWVFAIDDPESGYVGGWMKN
VLEFHDFAGGTAVHMNAGASGLALAMVLGRRHSMAVRPHNLPLILIGAGLIIAGWFGFNGGTAGGANFLASYVVVTSLIA
AAGGMMGFMLVERVFSGKPTFFGSATGTIAGLVAITPAADAVSPLGAFAVGALGAVVSFWAISWKKGHRVDDSFDVFAVH
GMAGIAGALFVMLFGDPLAPAGVSGVFFGGELSLLWREPLAIIVTLAYAFGVTWLIATILNKFMTLRITSEAEYEGIDRA
EHAESAYHLNSNGIGMATRTNFGPEIPEETVPDAVQVGVDKQKIADTRKASK
>Mature_452_residues
MDPSDLAWILAAFALVSLMFPGLSLLYGGMLGGQHVLNTFMMVMSSLGIISLVYIFYGHGLVLGNSIGGWGIIGNPLEYF
GFRNIMEDDGTGDLMWAGFYILFAAISLALVSSGAAGRMRFGAWLVFGVLWFTFVYAPLAHWVFAIDDPESGYVGGWMKN
VLEFHDFAGGTAVHMNAGASGLALAMVLGRRHSMAVRPHNLPLILIGAGLIIAGWFGFNGGTAGGANFLASYVVVTSLIA
AAGGMMGFMLVERVFSGKPTFFGSATGTIAGLVAITPAADAVSPLGAFAVGALGAVVSFWAISWKKGHRVDDSFDVFAVH
GMAGIAGALFVMLFGDPLAPAGVSGVFFGGELSLLWREPLAIIVTLAYAFGVTWLIATILNKFMTLRITSEAEYEGIDRA
EHAESAYHLNSNGIGMATRTNFGPEIPEETVPDAVQVGVDKQKIADTRKASK

Specific function: Involved in the uptake of ammonia and methylammonia. Utilizes uniport of ammonium and uses the membrane potential as driving force [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=420, Percent_Identity=34.2857142857143, Blast_Score=212, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17550182, Length=346, Percent_Identity=24.8554913294798, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17550180, Length=349, Percent_Identity=24.9283667621776, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI133931100, Length=454, Percent_Identity=25.5506607929515, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32564042, Length=429, Percent_Identity=26.1072261072261, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324187, Length=476, Percent_Identity=29.8319327731092, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6325396, Length=477, Percent_Identity=28.7211740041929, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321559, Length=422, Percent_Identity=28.9099526066351, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI161078314, Length=422, Percent_Identity=27.4881516587678, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47736; Mature: 47736

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPSDLAWILAAFALVSLMFPGLSLLYGGMLGGQHVLNTFMMVMSSLGIISLVYIFYGHG
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LVLGNSIGGWGIIGNPLEYFGFRNIMEDDGTGDLMWAGFYILFAAISLALVSSGAAGRMR
EEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
FGAWLVFGVLWFTFVYAPLAHWVFAIDDPESGYVGGWMKNVLEFHDFAGGTAVHMNAGAS
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCH
GLALAMVLGRRHSMAVRPHNLPLILIGAGLIIAGWFGFNGGTAGGANFLASYVVVTSLIA
HHHHHHHHCCCCCCEECCCCCCEEEEECHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHH
AAGGMMGFMLVERVFSGKPTFFGSATGTIAGLVAITPAADAVSPLGAFAVGALGAVVSFW
HHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHH
AISWKKGHRVDDSFDVFAVHGMAGIAGALFVMLFGDPLAPAGVSGVFFGGELSLLWREPL
HHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEHHHHHH
AIIVTLAYAFGVTWLIATILNKFMTLRITSEAEYEGIDRAEHAESAYHLNSNGIGMATRT
HHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHCHHEEECCCCCCEEEEC
NFGPEIPEETVPDAVQVGVDKQKIADTRKASK
CCCCCCCHHHCCCHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure
MDPSDLAWILAAFALVSLMFPGLSLLYGGMLGGQHVLNTFMMVMSSLGIISLVYIFYGHG
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LVLGNSIGGWGIIGNPLEYFGFRNIMEDDGTGDLMWAGFYILFAAISLALVSSGAAGRMR
EEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
FGAWLVFGVLWFTFVYAPLAHWVFAIDDPESGYVGGWMKNVLEFHDFAGGTAVHMNAGAS
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCH
GLALAMVLGRRHSMAVRPHNLPLILIGAGLIIAGWFGFNGGTAGGANFLASYVVVTSLIA
HHHHHHHHCCCCCCEECCCCCCEEEEECHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHH
AAGGMMGFMLVERVFSGKPTFFGSATGTIAGLVAITPAADAVSPLGAFAVGALGAVVSFW
HHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHH
AISWKKGHRVDDSFDVFAVHGMAGIAGALFVMLFGDPLAPAGVSGVFFGGELSLLWREPL
HHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEHHHHHH
AIIVTLAYAFGVTWLIATILNKFMTLRITSEAEYEGIDRAEHAESAYHLNSNGIGMATRT
HHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHCHHEEECCCCCCEEEEC
NFGPEIPEETVPDAVQVGVDKQKIADTRKASK
CCCCCCCHHHCCCHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8621394; 12948626; 2666264 [H]