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The map label for this gene is mshC

Identifier: 145295647

GI number: 145295647

Start: 1740745

End: 1742007

Strand: Reverse

Name: mshC

Synonym: cgR_1574

Alternate gene names: 145295647

Gene position: 1742007-1740745 (Counterclockwise)

Preceding gene: 145295648

Following gene: 145295646

Centisome position: 52.56

GC content: 58.04

Gene sequence:

>1263_bases
ATGCAATCTTGGCCTACACCTGAAGTACCTGCTCTCGCTGGCACGCCTGTGCCTTTGGAATTGTTCGACACTGCAGATCA
GGAAGTACGCCTGGTTGAAACCCCACCTGCCGGATCCGACACACCGGTTGGCATGTACGTCTGTGGCATCACTCCCTACG
ACTCCACACACTTGGGGCACGCGGCGACATACCTCGCTTTCGATCTGATCTACCGCATCCTGCTGGACAATGATCACGAT
GTCCACTACGTCCAAAACATCACCGATGTCGATGACCCTCTGTTTGAACGCGCAGCCCGCGACGGCGTCGACTGGCGCGA
CCTCGGCACCAGCCAAATCAATCTCTTCCGCAGCGATATGGAAGCCCTGAGCATCATTCCGCCGAAGGACTACATCGGTG
CGATTGAGTCCATCGACGAGGTCATTGAGATGGTCAAGACGCTTCTCGACGAAGGCGCCGCATACATCGTCGAGGACGCC
GAATATCCAGATGTCTACGCATCAATCAACGCCACAGACAAATTTGGCTACGAGTCCAATTACGATGCAGCGACCATGGC
TGAGTTCTTCGCAGAACGCGGCGGCGACCCAGACCGTCCCGGCAAGAAAAACCCTATGGACGCCCTCCTGTGGCGCGCAG
CCCGCGAAGGTGAACCAAGCTGGGAATCCCCATTCGGCGCAGGTCGTCCTGGCTGGCACATCGAGTGTTCAGCAATCGCT
ACCAACCGACTAGGCCACAGCTTTGATATCCAAGGTGGCGGCTCTGACCTGATCTTCCCTCACCACGAGTTCTCCGCAGC
GCACGCCGAAGCAGCTCACGGTGTCGAGCGCATGGCTAAGCACTACGTCCACGCTGGCATGATATCCCAAGACGGCGTGA
AAATGTCCAAGTCTTTGGGCAACCTGGAATTTGTTTCCCGACTCACCGCTGCAGGCCATGAGCCCGGCGCGATCCGCCTT
GGTGTTTTTGCAAACCACTACCGTGGCAACCGCGATTGGAACGCAGAAAGCCTCGCTACTGCAGAACAGCGCCTGGCAAC
CTGGCGTGAGGCAGCACGAGCTGCAACCAATAGGGAAGACGCCATTGCAGTTGTGGAGCAGCTCCGTGCGCACCTATCTG
CTGACCTTGATACCCCGGGCGCGCTCGCAGCGGTAGATAATTGGGCAGCGGGTATCGACACCACGGCAGGTTCAAAAGAG
TTCACCGAGGTCGGAAACATCGTGGTCGCAGCCATTGATGCCCTCCTGGGCGTGCAGCTCTAG

Upstream 100 bases:

>100_bases
CGAACATAATTTCATAAGTTTCTAGGACGTGTCTCCGCGCTGAACGCATGCAAATAGCTTGTCGCGGAAATAATTACGGA
AAGAAGATAGAGTAAAACCC

Downstream 100 bases:

>100_bases
GAAATTTCAGGAACAATACATCTCATGACCATTCGCGCCACTTTCCAGCCTTCCGTAGATGAATTCATCTCCACTCTCGA
AGAGTTTGCAACAGGTTCAT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: L-Cys:GlcN-Ins ligase; Mycothiol ligase; MSH ligase

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MQSWPTPEVPALAGTPVPLELFDTADQEVRLVETPPAGSDTPVGMYVCGITPYDSTHLGHAATYLAFDLIYRILLDNDHD
VHYVQNITDVDDPLFERAARDGVDWRDLGTSQINLFRSDMEALSIIPPKDYIGAIESIDEVIEMVKTLLDEGAAYIVEDA
EYPDVYASINATDKFGYESNYDAATMAEFFAERGGDPDRPGKKNPMDALLWRAAREGEPSWESPFGAGRPGWHIECSAIA
TNRLGHSFDIQGGGSDLIFPHHEFSAAHAEAAHGVERMAKHYVHAGMISQDGVKMSKSLGNLEFVSRLTAAGHEPGAIRL
GVFANHYRGNRDWNAESLATAEQRLATWREAARAATNREDAIAVVEQLRAHLSADLDTPGALAAVDNWAAGIDTTAGSKE
FTEVGNIVVAAIDALLGVQL

Sequences:

>Translated_420_residues
MQSWPTPEVPALAGTPVPLELFDTADQEVRLVETPPAGSDTPVGMYVCGITPYDSTHLGHAATYLAFDLIYRILLDNDHD
VHYVQNITDVDDPLFERAARDGVDWRDLGTSQINLFRSDMEALSIIPPKDYIGAIESIDEVIEMVKTLLDEGAAYIVEDA
EYPDVYASINATDKFGYESNYDAATMAEFFAERGGDPDRPGKKNPMDALLWRAAREGEPSWESPFGAGRPGWHIECSAIA
TNRLGHSFDIQGGGSDLIFPHHEFSAAHAEAAHGVERMAKHYVHAGMISQDGVKMSKSLGNLEFVSRLTAAGHEPGAIRL
GVFANHYRGNRDWNAESLATAEQRLATWREAARAATNREDAIAVVEQLRAHLSADLDTPGALAAVDNWAAGIDTTAGSKE
FTEVGNIVVAAIDALLGVQL
>Mature_420_residues
MQSWPTPEVPALAGTPVPLELFDTADQEVRLVETPPAGSDTPVGMYVCGITPYDSTHLGHAATYLAFDLIYRILLDNDHD
VHYVQNITDVDDPLFERAARDGVDWRDLGTSQINLFRSDMEALSIIPPKDYIGAIESIDEVIEMVKTLLDEGAAYIVEDA
EYPDVYASINATDKFGYESNYDAATMAEFFAERGGDPDRPGKKNPMDALLWRAAREGEPSWESPFGAGRPGWHIECSAIA
TNRLGHSFDIQGGGSDLIFPHHEFSAAHAEAAHGVERMAKHYVHAGMISQDGVKMSKSLGNLEFVSRLTAAGHEPGAIRL
GVFANHYRGNRDWNAESLATAEQRLATWREAARAATNREDAIAVVEQLRAHLSADLDTPGALAAVDNWAAGIDTTAGSKE
FTEVGNIVVAAIDALLGVQL

Specific function: Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily

Homologues:

Organism=Homo sapiens, GI13375695, Length=360, Percent_Identity=32.7777777777778, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI303304987, Length=235, Percent_Identity=35.7446808510638, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI21269877, Length=235, Percent_Identity=35.7446808510638, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI62240992, Length=235, Percent_Identity=35.7446808510638, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI10835051, Length=235, Percent_Identity=35.7446808510638, Blast_Score=148, Evalue=8e-36,
Organism=Escherichia coli, GI1786737, Length=380, Percent_Identity=36.5789473684211, Blast_Score=231, Evalue=8e-62,
Organism=Caenorhabditis elegans, GI17509795, Length=258, Percent_Identity=31.0077519379845, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17509797, Length=258, Percent_Identity=31.0077519379845, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324082, Length=295, Percent_Identity=32.2033898305085, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922218, Length=312, Percent_Identity=35.8974358974359, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI19922364, Length=273, Percent_Identity=33.6996336996337, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSHC_CORGB (A4QEA0)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138468.1
- STRING:   A4QEA0
- GeneID:   4992970
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1574
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   ALFREDM
- ProtClustDB:   PRK12418
- GO:   GO:0005737
- HAMAP:   MF_01697
- InterPro:   IPR015803
- InterPro:   IPR017812
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- TIGRFAMs:   TIGR03447

Pfam domain/function: PF01406 tRNA-synt_1e

EC number: =6.3.1.13

Molecular weight: Translated: 45645; Mature: 45645

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: BINDING 63-63 BINDING 232-232 BINDING 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSWPTPEVPALAGTPVPLELFDTADQEVRLVETPPAGSDTPVGMYVCGITPYDSTHLGH
CCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCHH
AATYLAFDLIYRILLDNDHDVHYVQNITDVDDPLFERAARDGVDWRDLGTSQINLFRSDM
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHH
EALSIIPPKDYIGAIESIDEVIEMVKTLLDEGAAYIVEDAEYPDVYASINATDKFGYESN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCCCCCC
YDAATMAEFFAERGGDPDRPGKKNPMDALLWRAAREGEPSWESPFGAGRPGWHIECSAIA
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEH
TNRLGHSFDIQGGGSDLIFPHHEFSAAHAEAAHGVERMAKHYVHAGMISQDGVKMSKSLG
HCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCC
NLEFVSRLTAAGHEPGAIRLGVFANHYRGNRDWNAESLATAEQRLATWREAARAATNRED
CHHHHHHHHHCCCCCCEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
AIAVVEQLRAHLSADLDTPGALAAVDNWAAGIDTTAGSKEFTEVGNIVVAAIDALLGVQL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSWPTPEVPALAGTPVPLELFDTADQEVRLVETPPAGSDTPVGMYVCGITPYDSTHLGH
CCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCCHH
AATYLAFDLIYRILLDNDHDVHYVQNITDVDDPLFERAARDGVDWRDLGTSQINLFRSDM
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHH
EALSIIPPKDYIGAIESIDEVIEMVKTLLDEGAAYIVEDAEYPDVYASINATDKFGYESN
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCCCCCC
YDAATMAEFFAERGGDPDRPGKKNPMDALLWRAAREGEPSWESPFGAGRPGWHIECSAIA
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEH
TNRLGHSFDIQGGGSDLIFPHHEFSAAHAEAAHGVERMAKHYVHAGMISQDGVKMSKSLG
HCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCC
NLEFVSRLTAAGHEPGAIRLGVFANHYRGNRDWNAESLATAEQRLATWREAARAATNRED
CHHHHHHHHHCCCCCCEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
AIAVVEQLRAHLSADLDTPGALAAVDNWAAGIDTTAGSKEFTEVGNIVVAAIDALLGVQL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA