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The map label for this gene is thiM

Identifier: 145295598

GI number: 145295598

Start: 1686447

End: 1687253

Strand: Reverse

Name: thiM

Synonym: cgR_1525

Alternate gene names: 145295598

Gene position: 1687253-1686447 (Counterclockwise)

Preceding gene: 145295599

Following gene: 145295597

Centisome position: 50.91

GC content: 58.49

Gene sequence:

>807_bases
GTGGCTAACTCGTTTTTGGATTCTTTAACTCTTGTTCGACAAAACACTCCCCTTGTTCAGTGTTTGACCAACTCTGTGGT
CATGCAATTCACAGCCAATGTGTTGCTTGCCGCGGGTGCGACCCCTGCGATGGTGGATACTCCAGCTGAATCGGCAGAAT
TCGCCGCTGTGGCCAATGGAGTGCTCATCAATGCGGGAACTCCTTCTGCGGAGCAATACCAAGGCATGACCAAGGCCATT
GAGGGTGCACGAAAAGCTGGCACACCATGGGTGTTAGACCCAGTTGCTGTGGGTGGGTTGTCGGAGAGGACCAAGTATGC
GGAGGGAATCGTCGATAAGCAGCCTGCCGCAATTCGTGGAAACGCCTCAGAGGTCGTGGCGCTTGCGGGGCTCGGCGCCG
GTGGGCGCGGCGTAGACGCGACCGATTCCGTGGAAGCGGCGTTGGAGGCGGCGCAATTGTTGGCCAAGCGCACTGGTGGC
GTCGTGGCTGTCTCTGGTGCGGAGGACTTGATTGTGTCTGCGGATCGGGTGACGTGGTTGCGTTCGGGGGATCCGATGTT
GCAGCTGGTGATTGGCACTGGATGCTCTTTGGGCGCGCTGACAGCTGCATATCTAGGCGCCACGGTTGACTCAGATATTT
CCGCGCACGATGCTGTGTTGGCTGCGCATGCCCATGTGGGTGCTGCTGGCCAGATTGCAGCACAGAAGGCATCGGCGCCA
GGCAGCTTTGCGGTGGCGTTTATTGATGCGCTTTATGACGTGGATGCCCAGGCTGTGGCCTCGTTGGTTGATGTGCGAGA
GGCCTGA

Upstream 100 bases:

>100_bases
CACTAGGCACGGGGTGCCAACCGGATGGAAAAATTCCGGGGGCTGAGAAAACACCCGTTGAACCTGCTCTAGCTCGTACT
AGCGAAGGGATGGCCTTAAC

Downstream 100 bases:

>100_bases
AAAGTACGTGACTGATTTTTCTTTGTATCTGGTCACCGATCCCGTTTTGGGTGGCGGGCCAGAAAAAGTAGCTGGAATTG
TTGACAGCGCAATTTCCGGC

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MANSFLDSLTLVRQNTPLVQCLTNSVVMQFTANVLLAAGATPAMVDTPAESAEFAAVANGVLINAGTPSAEQYQGMTKAI
EGARKAGTPWVLDPVAVGGLSERTKYAEGIVDKQPAAIRGNASEVVALAGLGAGGRGVDATDSVEAALEAAQLLAKRTGG
VVAVSGAEDLIVSADRVTWLRSGDPMLQLVIGTGCSLGALTAAYLGATVDSDISAHDAVLAAHAHVGAAGQIAAQKASAP
GSFAVAFIDALYDVDAQAVASLVDVREA

Sequences:

>Translated_268_residues
MANSFLDSLTLVRQNTPLVQCLTNSVVMQFTANVLLAAGATPAMVDTPAESAEFAAVANGVLINAGTPSAEQYQGMTKAI
EGARKAGTPWVLDPVAVGGLSERTKYAEGIVDKQPAAIRGNASEVVALAGLGAGGRGVDATDSVEAALEAAQLLAKRTGG
VVAVSGAEDLIVSADRVTWLRSGDPMLQLVIGTGCSLGALTAAYLGATVDSDISAHDAVLAAHAHVGAAGQIAAQKASAP
GSFAVAFIDALYDVDAQAVASLVDVREA
>Mature_267_residues
ANSFLDSLTLVRQNTPLVQCLTNSVVMQFTANVLLAAGATPAMVDTPAESAEFAAVANGVLINAGTPSAEQYQGMTKAIE
GARKAGTPWVLDPVAVGGLSERTKYAEGIVDKQPAAIRGNASEVVALAGLGAGGRGVDATDSVEAALEAAQLLAKRTGGV
VAVSGAEDLIVSADRVTWLRSGDPMLQLVIGTGCSLGALTAAYLGATVDSDISAHDAVLAAHAHVGAAGQIAAQKASAPG
SFAVAFIDALYDVDAQAVASLVDVREA

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=248, Percent_Identity=45.1612903225806, Blast_Score=210, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6325042, Length=277, Percent_Identity=29.2418772563177, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_CORGB (A4QE51)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138419.1
- ProteinModelPortal:   A4QE51
- SMR:   A4QE51
- STRING:   A4QE51
- GeneID:   4993933
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1525
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 26884; Mature: 26752

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 119-119 BINDING 165-165 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSFLDSLTLVRQNTPLVQCLTNSVVMQFTANVLLAAGATPAMVDTPAESAEFAAVANG
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEEECCCCHHHHHHHHCC
VLINAGTPSAEQYQGMTKAIEGARKAGTPWVLDPVAVGGLSERTKYAEGIVDKQPAAIRG
EEEECCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCHHHHHHHHCCCCCCCCEECC
NASEVVALAGLGAGGRGVDATDSVEAALEAAQLLAKRTGGVVAVSGAEDLIVSADRVTWL
CHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCEEEE
RSGDPMLQLVIGTGCSLGALTAAYLGATVDSDISAHDAVLAAHAHVGAAGQIAAQKASAP
ECCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCC
GSFAVAFIDALYDVDAQAVASLVDVREA
CHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
ANSFLDSLTLVRQNTPLVQCLTNSVVMQFTANVLLAAGATPAMVDTPAESAEFAAVANG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCCEEECCCCHHHHHHHHCC
VLINAGTPSAEQYQGMTKAIEGARKAGTPWVLDPVAVGGLSERTKYAEGIVDKQPAAIRG
EEEECCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCHHHHHHHHCCCCCCCCEECC
NASEVVALAGLGAGGRGVDATDSVEAALEAAQLLAKRTGGVVAVSGAEDLIVSADRVTWL
CHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCEEEE
RSGDPMLQLVIGTGCSLGALTAAYLGATVDSDISAHDAVLAAHAHVGAAGQIAAQKASAP
ECCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCC
GSFAVAFIDALYDVDAQAVASLVDVREA
CHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA