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The map label for this gene is ppnK [H]

Identifier: 145295547

GI number: 145295547

Start: 1632438

End: 1633400

Strand: Direct

Name: ppnK [H]

Synonym: cgR_1474

Alternate gene names: 145295547

Gene position: 1632438-1633400 (Clockwise)

Preceding gene: 145295546

Following gene: 145295548

Centisome position: 49.26

GC content: 55.14

Gene sequence:

>963_bases
ATGACTGCACCCACGAACGCTGGGGAACTCAGGCGAGTTTTGCTGGTTCCACACACCGGGCGTTCTTCCAATATTGAATC
CGCCATCTTGGCAGCCAAGCTGCTCGATGATGCTGGAATCGATGTGAGGGTGCTGATCAACGATGCAGATGATCCAATTG
CAGAGCACCCCGTTTTAGGCCGTTTCACCCATGTCAGGCACGCTGCCGACGCCGCTGAAGGCGCAGAGCTGGTCCTGGTT
CTGGGTGGTGATGGCACCTTCCTTCGCGCAGCAGATATGGCCCACGCTGTTGATTTGCCTGTTTTGGGCATCAACCTAGG
CCATGTGGGATTCTTGGCTGAATGGGAGTCTGATTCGCTTGAAGAGGCACTCAAACGTGTGATCGACCGCGATTACCGTA
TTGAAGATCGCATGACCTTAACTGTCGTTGTCCTCGACGGCGGCGGAGAAGAAATCGGCCGAGGCTGGGCTCTCAATGAG
GTCAGTATTGAAAACTTAAACCGCAGGGGAGTGCTCGATGCAACCCTCGAGGTAGATGCACGACCAGTTGCTTCCTTTGG
TTGCGATGGCGTGCTGATTTCCACCCCAACCGGCTCCACCGCTTATGCATTTTCCGCCGGTGGTCCTGTACTGTGGCCAG
AACTCGATGCCATCTTGGTGGTTCCTAATAACGCCCACGCGCTGTTTACCAAACCGCTGGTTGTGAGCCCAAAATCCACC
GTAGCTGTGGAATCCAATTCAGATACTTCAGCAGCGATGGCCGTCATGGATGGTTTCCGTCCCATTCCTATGCCTCCTGG
ATCCCGTGTTGAGGTCACCAGGGGTGAGCGTCCCGTGCGTTGGGTAAGGCTTGATTCTTCACCGTTTACCGACCGACTTG
TGAGCAAATTAAGGCTCCCCGTTACCGGTTGGCGGGGTCCGCAAAAACAGGCGGAAAATAAAGATCCCAGGTCAGCGGGG
TAA

Upstream 100 bases:

>100_bases
AATACTTCCTGTGGCTGGTTAAAGATGGTGGCGCTTCAATGCCGGATGACCAGCAATTGTCGGCAATGATTGACACGGCT
GTAAAGGAAGGTCCGCAATA

Downstream 100 bases:

>100_bases
TTCGAAAACCATTCGAACAATTTTCGAGGATTTAGAAAAAACGTTCGCATAAATTGTTAGAACTGATGTACACTTTGAGG
CATGCTCGTAGACATTGCTA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTAPTNAGELRRVLLVPHTGRSSNIESAILAAKLLDDAGIDVRVLINDADDPIAEHPVLGRFTHVRHAADAAEGAELVLV
LGGDGTFLRAADMAHAVDLPVLGINLGHVGFLAEWESDSLEEALKRVIDRDYRIEDRMTLTVVVLDGGGEEIGRGWALNE
VSIENLNRRGVLDATLEVDARPVASFGCDGVLISTPTGSTAYAFSAGGPVLWPELDAILVVPNNAHALFTKPLVVSPKST
VAVESNSDTSAAMAVMDGFRPIPMPPGSRVEVTRGERPVRWVRLDSSPFTDRLVSKLRLPVTGWRGPQKQAENKDPRSAG

Sequences:

>Translated_320_residues
MTAPTNAGELRRVLLVPHTGRSSNIESAILAAKLLDDAGIDVRVLINDADDPIAEHPVLGRFTHVRHAADAAEGAELVLV
LGGDGTFLRAADMAHAVDLPVLGINLGHVGFLAEWESDSLEEALKRVIDRDYRIEDRMTLTVVVLDGGGEEIGRGWALNE
VSIENLNRRGVLDATLEVDARPVASFGCDGVLISTPTGSTAYAFSAGGPVLWPELDAILVVPNNAHALFTKPLVVSPKST
VAVESNSDTSAAMAVMDGFRPIPMPPGSRVEVTRGERPVRWVRLDSSPFTDRLVSKLRLPVTGWRGPQKQAENKDPRSAG
>Mature_319_residues
TAPTNAGELRRVLLVPHTGRSSNIESAILAAKLLDDAGIDVRVLINDADDPIAEHPVLGRFTHVRHAADAAEGAELVLVL
GGDGTFLRAADMAHAVDLPVLGINLGHVGFLAEWESDSLEEALKRVIDRDYRIEDRMTLTVVVLDGGGEEIGRGWALNEV
SIENLNRRGVLDATLEVDARPVASFGCDGVLISTPTGSTAYAFSAGGPVLWPELDAILVVPNNAHALFTKPLVVSPKSTV
AVESNSDTSAAMAVMDGFRPIPMPPGSRVEVTRGERPVRWVRLDSSPFTDRLVSKLRLPVTGWRGPQKQAENKDPRSAG

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=236, Percent_Identity=32.2033898305085, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320794, Length=251, Percent_Identity=28.2868525896414, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322509, Length=229, Percent_Identity=26.6375545851528, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325068, Length=198, Percent_Identity=26.7676767676768, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28573832, Length=183, Percent_Identity=32.2404371584699, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161077047, Length=183, Percent_Identity=32.2404371584699, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573830, Length=183, Percent_Identity=32.2404371584699, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28573828, Length=183, Percent_Identity=32.2404371584699, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28573826, Length=183, Percent_Identity=32.2404371584699, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 34235; Mature: 34104

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPTNAGELRRVLLVPHTGRSSNIESAILAAKLLDDAGIDVRVLINDADDPIAEHPVLG
CCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHCCCCCC
RFTHVRHAADAAEGAELVLVLGGDGTFLRAADMAHAVDLPVLGINLGHVGFLAEWESDSL
HHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCHHHCCCCEEEEECCCEEEEEECCCHHH
EEALKRVIDRDYRIEDRMTLTVVVLDGGGEEIGRGWALNEVSIENLNRRGVLDATLEVDA
HHHHHHHHCCCCEECCCEEEEEEEECCCCHHHCCCEEECEEECCCCCCCCEEEEEEEECC
RPVASFGCDGVLISTPTGSTAYAFSAGGPVLWPELDAILVVPNNAHALFTKPLVVSPKST
CCHHHCCCCEEEEECCCCCEEEEEECCCCEECCCCCEEEEECCCCCEEEECCEEECCCCE
VAVESNSDTSAAMAVMDGFRPIPMPPGSRVEVTRGERPVRWVRLDSSPFTDRLVSKLRLP
EEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHCCC
VTGWRGPQKQAENKDPRSAG
CCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
TAPTNAGELRRVLLVPHTGRSSNIESAILAAKLLDDAGIDVRVLINDADDPIAEHPVLG
CCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHCCCCCC
RFTHVRHAADAAEGAELVLVLGGDGTFLRAADMAHAVDLPVLGINLGHVGFLAEWESDSL
HHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCHHHCCCCEEEEECCCEEEEEECCCHHH
EEALKRVIDRDYRIEDRMTLTVVVLDGGGEEIGRGWALNEVSIENLNRRGVLDATLEVDA
HHHHHHHHCCCCEECCCEEEEEEEECCCCHHHCCCEEECEEECCCCCCCCEEEEEEEECC
RPVASFGCDGVLISTPTGSTAYAFSAGGPVLWPELDAILVVPNNAHALFTKPLVVSPKST
CCHHHCCCCEEEEECCCCCEEEEEECCCCEECCCCCEEEEECCCCCEEEECCEEECCCCE
VAVESNSDTSAAMAVMDGFRPIPMPPGSRVEVTRGERPVRWVRLDSSPFTDRLVSKLRLP
EEEECCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHCCC
VTGWRGPQKQAENKDPRSAG
CCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]