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The map label for this gene is rihB [H]

Identifier: 145295501

GI number: 145295501

Start: 1572087

End: 1573025

Strand: Reverse

Name: rihB [H]

Synonym: cgR_1429

Alternate gene names: 145295501

Gene position: 1573025-1572087 (Counterclockwise)

Preceding gene: 145295502

Following gene: 145295500

Centisome position: 47.46

GC content: 56.55

Gene sequence:

>939_bases
ATGACCACCAAGATCATCCTCGACTGCGATCCAGGACACGATGACGCTGTAGCCATGCTGCTTTCAGCCGGCAGCCCCGA
AATTGAACTGCTGGGTATCACCACTGTCGGTGGCAACCAGACTTTGGACAAGGTCACCCATAACGCGCAGGTCGTGGCCA
CCATCGCTGATATCAATGCGCCCATCTACCGCGGTGTCACCCGACCACTGGTACGCCCCGTCGAGGTAGCCGAAGACATC
CACGGCGATACCGGCATGGAGATCCACAAATATGAGCTGCCTGAACCAACCAAACTGGTTGAAGGCACCCATGCGGTGGA
TTTCATCATCGACACCATCATGAACAACGAGCCCGGCACCGTAGCGCTGGTTCCCACCGGACCACTGACCAACATCGCGC
TGGCAGTCCGAAAAGAGCCACGCATCGCCGAGCGAGTCAAGGAAGTTGTCCTCATGGGCGGTGGCTACCACGTAGGAAAC
TGGACCGCCGTAGCCGAATTCAACATCAAGATCGACCCCGAAGCAGCCCACATCGTATTCAACGAAAAGTGGCCACTGAC
TATGGTCGGCCTCGACCTTACCCACCAGGCGCTCGCAACACCTGAGATCGAAGCCAAGTTCAACGAGCTGGGCACCGACG
TCGCCGACTTCGTCGTCGCGCTTTTCGACGCTTTCCGCAAGAATTACCAGGACGCACAGGGCTTTAATAACCCACCAGTA
CACGACCCTTGTGCTGTTGCATACCTTGTTGACCCAACCGTATTCACCACCCGCAAGGCACCACTCGACGTGGAGCTGTA
CGGCGCACTCACTACAGGTATGACCGTTGCTGATTTCCGCGCACCGGCTCCAGCAGATTGCACCACCCAAGTAGCTGTTG
ACCTGGACTTTGATAAATTCTGGAACATGGTGATCGATGCAGTAAAGCGCATCGGATAG

Upstream 100 bases:

>100_bases
CAGAATCAATCACCGATACAGTGGCAGCCAAAGTCCAGGCTGAAGAAGCCGCTCAAGCCGCCAGCTAAATCCTCATACTT
GAACTAAGGAGTTTTCCCCT

Downstream 100 bases:

>100_bases
ACCTGTTCACAAGGTTGTTAGAGGGCGTATCCGCACCTGATTGGTGAGGGTACGCCCTCCTTTTGTGGATATCGCTGCTA
GTGGACGTCGAAAAGCAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTTKIILDCDPGHDDAVAMLLSAGSPEIELLGITTVGGNQTLDKVTHNAQVVATIADINAPIYRGVTRPLVRPVEVAEDI
HGDTGMEIHKYELPEPTKLVEGTHAVDFIIDTIMNNEPGTVALVPTGPLTNIALAVRKEPRIAERVKEVVLMGGGYHVGN
WTAVAEFNIKIDPEAAHIVFNEKWPLTMVGLDLTHQALATPEIEAKFNELGTDVADFVVALFDAFRKNYQDAQGFNNPPV
HDPCAVAYLVDPTVFTTRKAPLDVELYGALTTGMTVADFRAPAPADCTTQVAVDLDFDKFWNMVIDAVKRIG

Sequences:

>Translated_312_residues
MTTKIILDCDPGHDDAVAMLLSAGSPEIELLGITTVGGNQTLDKVTHNAQVVATIADINAPIYRGVTRPLVRPVEVAEDI
HGDTGMEIHKYELPEPTKLVEGTHAVDFIIDTIMNNEPGTVALVPTGPLTNIALAVRKEPRIAERVKEVVLMGGGYHVGN
WTAVAEFNIKIDPEAAHIVFNEKWPLTMVGLDLTHQALATPEIEAKFNELGTDVADFVVALFDAFRKNYQDAQGFNNPPV
HDPCAVAYLVDPTVFTTRKAPLDVELYGALTTGMTVADFRAPAPADCTTQVAVDLDFDKFWNMVIDAVKRIG
>Mature_311_residues
TTKIILDCDPGHDDAVAMLLSAGSPEIELLGITTVGGNQTLDKVTHNAQVVATIADINAPIYRGVTRPLVRPVEVAEDIH
GDTGMEIHKYELPEPTKLVEGTHAVDFIIDTIMNNEPGTVALVPTGPLTNIALAVRKEPRIAERVKEVVLMGGGYHVGNW
TAVAEFNIKIDPEAAHIVFNEKWPLTMVGLDLTHQALATPEIEAKFNELGTDVADFVVALFDAFRKNYQDAQGFNNPPVH
DPCAVAYLVDPTVFTTRKAPLDVELYGALTTGMTVADFRAPAPADCTTQVAVDLDFDKFWNMVIDAVKRIG

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=306, Percent_Identity=42.483660130719, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1786871, Length=312, Percent_Identity=38.7820512820513, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1786213, Length=308, Percent_Identity=37.3376623376623, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI32565474, Length=191, Percent_Identity=37.696335078534, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17565698, Length=291, Percent_Identity=30.2405498281787, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320608, Length=328, Percent_Identity=35.0609756097561, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24641839, Length=290, Percent_Identity=30.3448275862069, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24641837, Length=198, Percent_Identity=33.8383838383838, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583915, Length=187, Percent_Identity=30.4812834224599, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 33849; Mature: 33718

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKIILDCDPGHDDAVAMLLSAGSPEIELLGITTVGGNQTLDKVTHNAQVVATIADINA
CCEEEEEECCCCCCHHHHHEECCCCCCEEEEEEEEECCCCHHHHHCCCCEEEEEEECCCC
PIYRGVTRPLVRPVEVAEDIHGDTGMEIHKYELPEPTKLVEGTHAVDFIIDTIMNNEPGT
HHHHCCHHHHCCHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCE
VALVPTGPLTNIALAVRKEPRIAERVKEVVLMGGGYHVGNWTAVAEFNIKIDPEAAHIVF
EEEEECCCCCCEEEEEECCCHHHHHHHHHEEECCCEEECCEEEEEEEEEEECCCEEEEEE
NEKWPLTMVGLDLTHQALATPEIEAKFNELGTDVADFVVALFDAFRKNYQDAQGFNNPPV
CCCCCEEEEECCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCCCCCCC
HDPCAVAYLVDPTVFTTRKAPLDVELYGALTTGMTVADFRAPAPADCTTQVAVDLDFDKF
CCCEEEEEEECCCEEECCCCCCCEEEEEEHCCCCEEECCCCCCCCCCCEEEEEEECHHHH
WNMVIDAVKRIG
HHHHHHHHHHCC
>Mature Secondary Structure 
TTKIILDCDPGHDDAVAMLLSAGSPEIELLGITTVGGNQTLDKVTHNAQVVATIADINA
CEEEEEECCCCCCHHHHHEECCCCCCEEEEEEEEECCCCHHHHHCCCCEEEEEEECCCC
PIYRGVTRPLVRPVEVAEDIHGDTGMEIHKYELPEPTKLVEGTHAVDFIIDTIMNNEPGT
HHHHCCHHHHCCHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHCCCCCE
VALVPTGPLTNIALAVRKEPRIAERVKEVVLMGGGYHVGNWTAVAEFNIKIDPEAAHIVF
EEEEECCCCCCEEEEEECCCHHHHHHHHHEEECCCEEECCEEEEEEEEEEECCCEEEEEE
NEKWPLTMVGLDLTHQALATPEIEAKFNELGTDVADFVVALFDAFRKNYQDAQGFNNPPV
CCCCCEEEEECCHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCCCCCCC
HDPCAVAYLVDPTVFTTRKAPLDVELYGALTTGMTVADFRAPAPADCTTQVAVDLDFDKF
CCCEEEEEEECCCEEECCCCCCCEEEEEEHCCCCEEECCCCCCCCCCCEEEEEEECHHHH
WNMVIDAVKRIG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA