Click here to switch to the map view.

The map label for this gene is pfkA [H]

Identifier: 145295389

GI number: 145295389

Start: 1457832

End: 1458872

Strand: Direct

Name: pfkA [H]

Synonym: cgR_1327

Alternate gene names: 145295389

Gene position: 1457832-1458872 (Clockwise)

Preceding gene: 145295387

Following gene: 145295390

Centisome position: 43.99

GC content: 54.76

Gene sequence:

>1041_bases
ATGGAAGACATGCGAATTGCTACTCTCACGTCAGGCGGCGACTGCCCCGGACTAAACGCCGTCATCCGAGGAATCGTCCG
CACAGCCAGCAATGAATTTGGCTCCACCGTCGTTGGTTATCAAGACGGTTGGGAAGGACTGTTAGCCGATCGTCGCGTAC
AGCTGTATGACGATGAAGATATTGACCGAATCCTCCTTCGAGGCGGCACCATTTTGGGCACTGGTCGCCTCCATCCGGAC
AAGTTTAAGGCCGGAATTGATCAGATTAAGGCCAACTTAGAAGACGCCGGCATCGATGCCCTTATTCCAATCGGTGGCGA
AGGAACCCTGAAGGGTGCCAAGTGGCTGTCTGATAACGGTATCCCTGTTGTCGGTGTCCCAAAGACCATTGACAATGACG
TGAATGGCACTGACTTCACCTTCGGTTTCGATACTGCTGTTGCAGTGGCTACCGACGCTGTTGACCGCCTGCACACCACC
GCTGAATCTCACAACCGTGTGATGATTGTGGAGGTCATGGGCCGCCACGTGGGTTGGATTGCTCTGCACGCAGGTATGGC
CGGCGGTGCTCACTACACCGTTATCCCAGAAGTTCCTTTCGATATTGCAGAGATCTGCAAGGCGATGGAACGTCGCTTCC
AGATGGGCGAGAAGTACGGCATTATCGTCGTTGCGGAAGGTGCATTGCCACGCGAAGGCACCATGGAACTTCGTGAAGGC
CACATTGACCAGTTCGGCCACAAGACCTTCACCGGAATCGGCCAGCAGATTGCTGATGAAATCCATGCGCGCCTCGGTCA
CGATGTTCGTACCACCGTTCTTGGCCACATTCAGCGTGGTGGAACCCCGACTGCTTTCGACCGTGTTCTGGCCACTCGTT
ATGGTGTTCGGGCAGCTCGTGCATGCCATGAGGGAAGCTTTGACAAGGTTGTTGCCTTGAAGGGTGAGCGCATCGAGATG
ATCACCTTTGAAGATGCCGTCGGAACCTTGAAGGAAGTTCCATTCGAGCGCTGGGTTACTGCCCAGGCAATGTTTGGATA
G

Upstream 100 bases:

>100_bases
TTAAATAAGGTGGTCAGAGACAATTTTTCGGCCGGTCAACCCCTGTGATTTTCTTATTTTTGGGTGATTGTTCCGGCGCG
GGTGTTGTGATGGGTTTAAT

Downstream 100 bases:

>100_bases
TTTTTCGGGCTTTTATCAACAGCCAATAACAGCTCTTTCGCCCATTGAGGTGGAGGGGCTGTTTTTTCATGCCGTAAGGA
AGGTGCAAGTAAGTGAAATC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MEDMRIATLTSGGDCPGLNAVIRGIVRTASNEFGSTVVGYQDGWEGLLADRRVQLYDDEDIDRILLRGGTILGTGRLHPD
KFKAGIDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPVVGVPKTIDNDVNGTDFTFGFDTAVAVATDAVDRLHTT
AESHNRVMIVEVMGRHVGWIALHAGMAGGAHYTVIPEVPFDIAEICKAMERRFQMGEKYGIIVVAEGALPREGTMELREG
HIDQFGHKTFTGIGQQIADEIHARLGHDVRTTVLGHIQRGGTPTAFDRVLATRYGVRAARACHEGSFDKVVALKGERIEM
ITFEDAVGTLKEVPFERWVTAQAMFG

Sequences:

>Translated_346_residues
MEDMRIATLTSGGDCPGLNAVIRGIVRTASNEFGSTVVGYQDGWEGLLADRRVQLYDDEDIDRILLRGGTILGTGRLHPD
KFKAGIDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPVVGVPKTIDNDVNGTDFTFGFDTAVAVATDAVDRLHTT
AESHNRVMIVEVMGRHVGWIALHAGMAGGAHYTVIPEVPFDIAEICKAMERRFQMGEKYGIIVVAEGALPREGTMELREG
HIDQFGHKTFTGIGQQIADEIHARLGHDVRTTVLGHIQRGGTPTAFDRVLATRYGVRAARACHEGSFDKVVALKGERIEM
ITFEDAVGTLKEVPFERWVTAQAMFG
>Mature_346_residues
MEDMRIATLTSGGDCPGLNAVIRGIVRTASNEFGSTVVGYQDGWEGLLADRRVQLYDDEDIDRILLRGGTILGTGRLHPD
KFKAGIDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPVVGVPKTIDNDVNGTDFTFGFDTAVAVATDAVDRLHTT
AESHNRVMIVEVMGRHVGWIALHAGMAGGAHYTVIPEVPFDIAEICKAMERRFQMGEKYGIIVVAEGALPREGTMELREG
HIDQFGHKTFTGIGQQIADEIHARLGHDVRTTVLGHIQRGGTPTAFDRVLATRYGVRAARACHEGSFDKVVALKGERIEM
ITFEDAVGTLKEVPFERWVTAQAMFG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=358, Percent_Identity=36.0335195530726, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI266453748, Length=358, Percent_Identity=36.0335195530726, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI4505749, Length=358, Percent_Identity=36.0335195530726, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI266453619, Length=358, Percent_Identity=36.0335195530726, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI11321601, Length=358, Percent_Identity=34.9162011173184, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI48762920, Length=358, Percent_Identity=33.7988826815642, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI1790350, Length=333, Percent_Identity=39.3393393393393, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI25147584, Length=328, Percent_Identity=34.1463414634146, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17558788, Length=334, Percent_Identity=31.437125748503, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI72003601, Length=304, Percent_Identity=30.2631578947368, Blast_Score=127, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6321679, Length=331, Percent_Identity=38.368580060423, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6323861, Length=360, Percent_Identity=35, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI28573326, Length=379, Percent_Identity=34.8284960422164, Blast_Score=194, Evalue=8e-50,
Organism=Drosophila melanogaster, GI17647809, Length=380, Percent_Identity=34.4736842105263, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24652337, Length=379, Percent_Identity=34.5646437994723, Blast_Score=192, Evalue=4e-49,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 37505; Mature: 37505

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDMRIATLTSGGDCPGLNAVIRGIVRTASNEFGSTVVGYQDGWEGLLADRRVQLYDDED
CCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHCCEEECCCCCCHHHHHCCEEEEECCCC
IDRILLRGGTILGTGRLHPDKFKAGIDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNG
HHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCCC
IPVVGVPKTIDNDVNGTDFTFGFDTAVAVATDAVDRLHTTAESHNRVMIVEVMGRHVGWI
CEEEECCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEE
ALHAGMAGGAHYTVIPEVPFDIAEICKAMERRFQMGEKYGIIVVAEGALPREGTMELREG
EEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHC
HIDQFGHKTFTGIGQQIADEIHARLGHDVRTTVLGHIQRGGTPTAFDRVLATRYGVRAAR
CHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ACHEGSFDKVVALKGERIEMITFEDAVGTLKEVPFERWVTAQAMFG
HHCCCCCCEEEEECCCEEEEEEEHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MEDMRIATLTSGGDCPGLNAVIRGIVRTASNEFGSTVVGYQDGWEGLLADRRVQLYDDED
CCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHCCEEECCCCCCHHHHHCCEEEEECCCC
IDRILLRGGTILGTGRLHPDKFKAGIDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNG
HHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCCC
IPVVGVPKTIDNDVNGTDFTFGFDTAVAVATDAVDRLHTTAESHNRVMIVEVMGRHVGWI
CEEEECCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEE
ALHAGMAGGAHYTVIPEVPFDIAEICKAMERRFQMGEKYGIIVVAEGALPREGTMELREG
EEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHC
HIDQFGHKTFTGIGQQIADEIHARLGHDVRTTVLGHIQRGGTPTAFDRVLATRYGVRAAR
CHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ACHEGSFDKVVALKGERIEMITFEDAVGTLKEVPFERWVTAQAMFG
HHCCCCCCEEEEECCCEEEEEEEHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]