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The map label for this gene is pth1 [H]

Identifier: 145295096

GI number: 145295096

Start: 1148055

End: 1148597

Strand: Reverse

Name: pth1 [H]

Synonym: cgR_1039

Alternate gene names: 145295096

Gene position: 1148597-1148055 (Counterclockwise)

Preceding gene: 145295097

Following gene: 145295093

Centisome position: 34.66

GC content: 51.2

Gene sequence:

>543_bases
ATGGGGGATGTGAATAACTCTCCCCTTTTAGTTGTTGGCCTGGGAAATCCGGGGCCGAAATACGTTGGTACCCGACACAA
TATTGGCTTTGAGGTTGCAGAAGAACTAGTATCGCGCAGCTTTGGATCATTTAGCGCGCATAAGCGCTCCAACACCGATA
TCGCGCAGCTTCCTGGGCTAATTGTTGCTAAGCCACGCAGCTTTATGAACCTGTCGGGAACTCCGATTCGGGCACTGTGT
GATTTCTTTAAGATTTCCCCAGCCAATGTCATCGTGGTGCATGATGAATTGGAGCTTGATTTCGGCTCAGTGAAGCTACG
TCAGGGTGGCGGGGATCATGGGCACAATGGTCTGAAATCCACGTCCAAATCTTTGGGAACTAAGGACTATTGGAAGCTCA
GCATGGGTATCGGTAGGCCACCGGGTCGGATGGATCCGGCAAGTTTTGTGTTGAAGCCTTTTGGCAAGCAAGAACTGGCG
GATATTCCCATCATGGCGGCTGACGCTGCAGATCTCGTCGAAAAGCATTTGCAGCAGGGCTAG

Upstream 100 bases:

>100_bases
CGTGCGGGGAAATTTAAAGTGATGACAGTGCACGATCATCATGGAAGTTTCCTCACATGGTGGCGGGGTTCGTTTTTGCT
TGTGGTGGACTCATGGTTGG

Downstream 100 bases:

>100_bases
CTACTTGCGCCGCGCCTCTTTGAGGCGGCGCGGCAGGTCGGCGTCGTCGTTTTCTAAGACCCCGGATTTGAGTAAGCGTG
TGTACACCACTGGGCAGTTG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH 1 [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MGDVNNSPLLVVGLGNPGPKYVGTRHNIGFEVAEELVSRSFGSFSAHKRSNTDIAQLPGLIVAKPRSFMNLSGTPIRALC
DFFKISPANVIVVHDELELDFGSVKLRQGGGDHGHNGLKSTSKSLGTKDYWKLSMGIGRPPGRMDPASFVLKPFGKQELA
DIPIMAADAADLVEKHLQQG

Sequences:

>Translated_180_residues
MGDVNNSPLLVVGLGNPGPKYVGTRHNIGFEVAEELVSRSFGSFSAHKRSNTDIAQLPGLIVAKPRSFMNLSGTPIRALC
DFFKISPANVIVVHDELELDFGSVKLRQGGGDHGHNGLKSTSKSLGTKDYWKLSMGIGRPPGRMDPASFVLKPFGKQELA
DIPIMAADAADLVEKHLQQG
>Mature_179_residues
GDVNNSPLLVVGLGNPGPKYVGTRHNIGFEVAEELVSRSFGSFSAHKRSNTDIAQLPGLIVAKPRSFMNLSGTPIRALCD
FFKISPANVIVVHDELELDFGSVKLRQGGGDHGHNGLKSTSKSLGTKDYWKLSMGIGRPPGRMDPASFVLKPFGKQELAD
IPIMAADAADLVEKHLQQG

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=177, Percent_Identity=32.7683615819209, Blast_Score=95, Evalue=4e-20,
Organism=Escherichia coli, GI1787455, Length=184, Percent_Identity=36.9565217391304, Blast_Score=81, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321983, Length=179, Percent_Identity=34.0782122905028, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 19320; Mature: 19189

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1 ; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDVNNSPLLVVGLGNPGPKYVGTRHNIGFEVAEELVSRSFGSFSAHKRSNTDIAQLPGL
CCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCE
IVAKPRSFMNLSGTPIRALCDFFKISPANVIVVHDELELDFGSVKLRQGGGDHGHNGLKS
EEECCCHHCCCCCCCHHHHHHHHHCCCCCEEEEECCEECCCCCEEEECCCCCCCCCCHHH
TSKSLGTKDYWKLSMGIGRPPGRMDPASFVLKPFGKQELADIPIMAADAADLVEKHLQQG
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCHHHCCCEEECCHHHHHHHHHHCC
>Mature Secondary Structure 
GDVNNSPLLVVGLGNPGPKYVGTRHNIGFEVAEELVSRSFGSFSAHKRSNTDIAQLPGL
CCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCE
IVAKPRSFMNLSGTPIRALCDFFKISPANVIVVHDELELDFGSVKLRQGGGDHGHNGLKS
EEECCCHHCCCCCCCHHHHHHHHHCCCCCEEEEECCEECCCCCEEEECCCCCCCCCCHHH
TSKSLGTKDYWKLSMGIGRPPGRMDPASFVLKPFGKQELADIPIMAADAADLVEKHLQQG
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCHHHCCCEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]