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The map label for this gene is smpB [H]

Identifier: 145294964

GI number: 145294964

Start: 1003002

End: 1003496

Strand: Direct

Name: smpB [H]

Synonym: cgR_0909

Alternate gene names: 145294964

Gene position: 1003002-1003496 (Clockwise)

Preceding gene: 145294963

Following gene: 145294965

Centisome position: 30.26

GC content: 53.33

Gene sequence:

>495_bases
ATGGCCAAGAAGAAAAAGAAAGTCGACGAAAATAACTCAGTTCTCGCGACCAATCGCAAGGCCCGCCATGACTACCACAT
CATTGATACGTGGGAGGCGGGCGTGGTGCTCTTAGGCACCGAAATCAAATCACTGCGCGAAGGTAAGGTATCCCTCGTGG
ATTCCTTTGCCACCATTGATAACGGAGAAATCTGGCTTCAGCATCTCCACATCCCGCAGTATTCCATGGGCTCCTGGACA
AACCACACGCCCAAGCGCACCCGCAAACTTTTGCTGCACCGCAACGAGATTGATTCCCTGATGGGTAAAGTCCGCGACGG
CAACCGCACGTTGGTTCCGCTCAAGCTTTACCTCAAAAACGGTCGCGTCAAACTCGAACTCGGACTCGCACAAGGTAAGC
AGGATTACGACAAGCGCCAAGATATCAAGCGTCGCACCGAAGAACGCGAAGTCACCCGTGAGCTCGGCCGTCGCATTAAG
GGAATCAACGCGTAA

Upstream 100 bases:

>100_bases
CATTGGCGTGGTGATCGCCGGCATTATCGCACAACTCACCCTGCGCTTCTACGTGAGGAAATAAGACTATTGAACTCCTT
GCCGTAGACTTAGAAAGACT

Downstream 100 bases:

>100_bases
ATGAGTATTCACATCGCAAAAGTCCACGACGTCCTCAAAGGTGAAAAAACCTACGGAACCACCATTTTGGTGGATCGGCT
CTGGCCACGCGGTGTGAAAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MAKKKKKVDENNSVLATNRKARHDYHIIDTWEAGVVLLGTEIKSLREGKVSLVDSFATIDNGEIWLQHLHIPQYSMGSWT
NHTPKRTRKLLLHRNEIDSLMGKVRDGNRTLVPLKLYLKNGRVKLELGLAQGKQDYDKRQDIKRRTEEREVTRELGRRIK
GINA

Sequences:

>Translated_164_residues
MAKKKKKVDENNSVLATNRKARHDYHIIDTWEAGVVLLGTEIKSLREGKVSLVDSFATIDNGEIWLQHLHIPQYSMGSWT
NHTPKRTRKLLLHRNEIDSLMGKVRDGNRTLVPLKLYLKNGRVKLELGLAQGKQDYDKRQDIKRRTEEREVTRELGRRIK
GINA
>Mature_163_residues
AKKKKKVDENNSVLATNRKARHDYHIIDTWEAGVVLLGTEIKSLREGKVSLVDSFATIDNGEIWLQHLHIPQYSMGSWTN
HTPKRTRKLLLHRNEIDSLMGKVRDGNRTLVPLKLYLKNGRVKLELGLAQGKQDYDKRQDIKRRTEEREVTRELGRRIKG
INA

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=145, Percent_Identity=43.448275862069, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 19005; Mature: 18874

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKKKVDENNSVLATNRKARHDYHIIDTWEAGVVLLGTEIKSLREGKVSLVDSFATID
CCCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCEEEECCHHHHHHCCCHHHHHHHHCCC
NGEIWLQHLHIPQYSMGSWTNHTPKRTRKLLLHRNEIDSLMGKVRDGNRTLVPLKLYLKN
CCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEC
GRVKLELGLAQGKQDYDKRQDIKRRTEEREVTRELGRRIKGINA
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKKKKKVDENNSVLATNRKARHDYHIIDTWEAGVVLLGTEIKSLREGKVSLVDSFATID
CCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCEEEECCHHHHHHCCCHHHHHHHHCCC
NGEIWLQHLHIPQYSMGSWTNHTPKRTRKLLLHRNEIDSLMGKVRDGNRTLVPLKLYLKN
CCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEC
GRVKLELGLAQGKQDYDKRQDIKRRTEEREVTRELGRRIKGINA
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]