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The map label for this gene is prpB1 [H]

Identifier: 145294868

GI number: 145294868

Start: 902798

End: 903715

Strand: Direct

Name: prpB1 [H]

Synonym: cgR_0815

Alternate gene names: 145294868

Gene position: 902798-903715 (Clockwise)

Preceding gene: 145294867

Following gene: 145294869

Centisome position: 27.24

GC content: 59.26

Gene sequence:

>918_bases
ATGAATCTCTTTTCGAATGGTGTTGATGTGGGGAGGCGTCGACAAGCATTTAAAGCGGCACTCGCCGCACCCCACATCGC
CCGGCTGCCCGGCGCATTCTCCCCTCTGGTTGCGCGCTCCATCGAAGAAGCCGGCTTCGAAGGCGTCTACGTTTCCGGCG
CCGTCATAGCCGCTGACCTGGCACTACCCGATATCGGCTTGACGACGCTGACCGAAGTCGCCCACCGCGCGCGGCAAATT
GCGCGCGTCACAGACCTAGGAGTGCTTGTCGACGCCGACACCGGCTTTGGCGAACCCATGTCGGCCGCACGCACCGTCGC
CGAATTGGAGGACGCCGGTGTGGCCGGATGCCACCTTGAAGACCAAGTCAACCCCAAACGCTGCGGGCACTTGGACGGCA
AAGAAGTCGTGCGCACAGACGTGATGGTTCGACGCATCGCAGCCGCCGTCTCGGCCCGGCGCGACCCGAACTTTGTCATC
TGCGCCCGCACCGACGCCGCTGGAGTGGAAGGAATCGACGCCGCCCTTGAGCGCGCGAAAGCCTACTTGGATGCGGGCGC
CGACATGATTTTCACCGAAGCCCTCCACAGCGAAGCCGACTTCCGATACTTCCGGCACGCCATCCCTGATGCCTTGTTGC
TGGCGAATATGACCGAATTTGGCAAAACGACGCTGCTGTCAGCCGACGTGTTGGAAGAGATTGGCTACAACGCCGTGATC
TACCCCGTGACCACGCTGCGTATTGCCATGGGACAAGTAGAACAAGCACTAGCCGAAATCAAAGAACACGGTACCCAAGA
AGGATGGCTGGACCGCATGCAACACCGCAGCAGATTATATGAGCTCCTGCGATACGAAGACTACAACGTCTTTGACCAGC
ACATTTTCACCTACAGAAAAGGAGAAAACAATGAGTGA

Upstream 100 bases:

>100_bases
TCTTGCATGCCGTGCAAAGTCTGCCTGACCTGGATGATCTTGATCAGCTCAACATCGAAGTCGACATAAGCAACCAGGCC
GCGACGAAAGCGGGGCTGTT

Downstream 100 bases:

>100_bases
CAGCCAAGTCCGCAAAGGACTCAACGGCGTCATCTCTGACTACACAAGCATTTCCAAAGTGATGCCAGAGAGCAACTCGC
TGACTTACCGTGGCTACGCC

Product: hypothetical protein

Products: NA

Alternate protein names: 2-methylisocitrate lyase 1 [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNLFSNGVDVGRRRQAFKAALAAPHIARLPGAFSPLVARSIEEAGFEGVYVSGAVIAADLALPDIGLTTLTEVAHRARQI
ARVTDLGVLVDADTGFGEPMSAARTVAELEDAGVAGCHLEDQVNPKRCGHLDGKEVVRTDVMVRRIAAAVSARRDPNFVI
CARTDAAGVEGIDAALERAKAYLDAGADMIFTEALHSEADFRYFRHAIPDALLLANMTEFGKTTLLSADVLEEIGYNAVI
YPVTTLRIAMGQVEQALAEIKEHGTQEGWLDRMQHRSRLYELLRYEDYNVFDQHIFTYRKGENNE

Sequences:

>Translated_305_residues
MNLFSNGVDVGRRRQAFKAALAAPHIARLPGAFSPLVARSIEEAGFEGVYVSGAVIAADLALPDIGLTTLTEVAHRARQI
ARVTDLGVLVDADTGFGEPMSAARTVAELEDAGVAGCHLEDQVNPKRCGHLDGKEVVRTDVMVRRIAAAVSARRDPNFVI
CARTDAAGVEGIDAALERAKAYLDAGADMIFTEALHSEADFRYFRHAIPDALLLANMTEFGKTTLLSADVLEEIGYNAVI
YPVTTLRIAMGQVEQALAEIKEHGTQEGWLDRMQHRSRLYELLRYEDYNVFDQHIFTYRKGENNE
>Mature_305_residues
MNLFSNGVDVGRRRQAFKAALAAPHIARLPGAFSPLVARSIEEAGFEGVYVSGAVIAADLALPDIGLTTLTEVAHRARQI
ARVTDLGVLVDADTGFGEPMSAARTVAELEDAGVAGCHLEDQVNPKRCGHLDGKEVVRTDVMVRRIAAAVSARRDPNFVI
CARTDAAGVEGIDAALERAKAYLDAGADMIFTEALHSEADFRYFRHAIPDALLLANMTEFGKTTLLSADVLEEIGYNAVI
YPVTTLRIAMGQVEQALAEIKEHGTQEGWLDRMQHRSRLYELLRYEDYNVFDQHIFTYRKGENNE

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=290, Percent_Identity=43.1034482758621, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 33401; Mature: 33401

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFSNGVDVGRRRQAFKAALAAPHIARLPGAFSPLVARSIEEAGFEGVYVSGAVIAADL
CCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHH
ALPDIGLTTLTEVAHRARQIARVTDLGVLVDADTGFGEPMSAARTVAELEDAGVAGCHLE
CCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DQVNPKRCGHLDGKEVVRTDVMVRRIAAAVSARRDPNFVICARTDAAGVEGIDAALERAK
CCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AYLDAGADMIFTEALHSEADFRYFRHAIPDALLLANMTEFGKTTLLSADVLEEIGYNAVI
HHHHCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YPVTTLRIAMGQVEQALAEIKEHGTQEGWLDRMQHRSRLYELLRYEDYNVFDQHIFTYRK
EHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
GENNE
CCCCC
>Mature Secondary Structure
MNLFSNGVDVGRRRQAFKAALAAPHIARLPGAFSPLVARSIEEAGFEGVYVSGAVIAADL
CCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHCCCCCEEECCHHHHHHH
ALPDIGLTTLTEVAHRARQIARVTDLGVLVDADTGFGEPMSAARTVAELEDAGVAGCHLE
CCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCC
DQVNPKRCGHLDGKEVVRTDVMVRRIAAAVSARRDPNFVICARTDAAGVEGIDAALERAK
CCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AYLDAGADMIFTEALHSEADFRYFRHAIPDALLLANMTEFGKTTLLSADVLEEIGYNAVI
HHHHCCCHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
YPVTTLRIAMGQVEQALAEIKEHGTQEGWLDRMQHRSRLYELLRYEDYNVFDQHIFTYRK
EHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
GENNE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626 [H]