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The map label for this gene is prpD1 [H]

Identifier: 145294867

GI number: 145294867

Start: 901305

End: 902801

Strand: Direct

Name: prpD1 [H]

Synonym: cgR_0814

Alternate gene names: 145294867

Gene position: 901305-902801 (Clockwise)

Preceding gene: 145294866

Following gene: 145294868

Centisome position: 27.2

GC content: 55.64

Gene sequence:

>1497_bases
ATGCGCATCCACGATGTTTATACCCACCTTTCGGCCGATAACTTTCCCAAAGCAGAGCACCTTGCGTGGAAAATCTCCGA
GCTTGCCACCGACCCCGTGGAGGTGACACCGGATGTTTCGGAGATGATCATCAACCGGATCATCGACAACGCGGCGGTGT
CTGCTGCGTCGGTGTTGCGCCGGCCTGTGACTGTGGCCAGGCAACAGGCGCAGTCCCATCCGCGGGAAAAGGGCGGAAAA
GTTTTTGGAATTTCAGGCAGCTACTCACCAGAGTGGGCTGCCTTTGCTAATGGTGTGGCCGTACGTGAATTGGACTTCCA
CGATACATTTTTAGCAGCTGAATACTCCCATCCCGGCGACAATATTCCACCACTTCTTGCAGTAGCGCAGGCTCAGAGAA
GCAGCGGCAGGGATCTCATTCGGGGTATCGCCACGGCCTATGAGGTGCAGGTGGAATTGGTGAAGGGGATCTGCTTGCAT
GAGCACAAAATTGATCATGTAGCCCACCTTGGTCCCAGCGCTGCAGCGGGTTTGGGCACGTTGTTGCATGTAGATGAGGA
GACCATCTACCAGGCGATCGGCCAGGCATTGCACACCACGACGGCTACGAGGCAGTCGCGAAAAGGTGAGATTTCCAGCT
GGAAGGCGTTCGCGCCAGCGTTTGCGGGAAAGATGGCCATTGAGGCGATGGATCGTGCGATGCGTGGGGAGGGTTCGCCC
GCACCGATTTGGGAGGGCGAAGACGGGGTCATCGCGTGGCTGTTATCGGGCAAAGATCATGTTTATCATGTGCCATTGCC
GGAACACGGCGAGCCCAAGCTGGGGATTCTAGAGACTTACACAAAGGAACATTCAGCGGAATATCAATCGCAGGCACCGA
TTGATCTGGCGCGCAGGATGAAGCCACTGGTTGACGCGGCTGGCGGAACGGAACACATTGCAGAGATTGTGCTGCGCACC
AGTCACCATACGCATTATGTGATTGGCACTGGGGCGAACGATCCGCAGAAGATGGATCCGCAGGCCTCGCGTGAAACCCT
AGATCATTCCATCATGTACATTTTCGCCGTCGCGCTTCAAGATGGCGTGTGGCACCACGAGTTTTCCTACACCCGCAAGC
GTTCTACCCGCCCGGAAACTGTGGAGCTGTGGCACAAGATTCGCACCGTGGAGGATCCTGAATGGACGCGTCGATACCAT
TCTGATGATCCTGCAAAAAAGGCCTTTGGTGCGAAAGCAGTGATCACCATGGCTGATGGCACCCTGATTGAGGATGAATT
GGCTGTCGCGGATGCCCACCCGCTGGGTGCTCGGCCGTTTGCGCGGGAGAATTACATTGAAAAATTCCGCACACTCGCGC
AGGGGATTGTTATTGATTCAGAACAGGAACGCTTCTTGCATGCCGTGCAAAGTCTGCCTGACCTGGATGATCTTGATCAG
CTCAACATCGAAGTCGACATAAGCAACCAGGCCGCGACGAAAGCGGGGCTGTTATGA

Upstream 100 bases:

>100_bases
CGCGCAGAGCTAAACTGCGTGAGGTTGTGGCCTGTCACACATAATCAGCCTAGGGTGGGACTTTAAGGAAACAGTGCACA
AATAAATCTCAAGGAGCCCC

Downstream 100 bases:

>100_bases
ATCTCTTTTCGAATGGTGTTGATGTGGGGAGGCGTCGACAAGCATTTAAAGCGGCACTCGCCGCACCCCACATCGCCCGG
CTGCCCGGCGCATTCTCCCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MRIHDVYTHLSADNFPKAEHLAWKISELATDPVEVTPDVSEMIINRIIDNAAVSAASVLRRPVTVARQQAQSHPREKGGK
VFGISGSYSPEWAAFANGVAVRELDFHDTFLAAEYSHPGDNIPPLLAVAQAQRSSGRDLIRGIATAYEVQVELVKGICLH
EHKIDHVAHLGPSAAAGLGTLLHVDEETIYQAIGQALHTTTATRQSRKGEISSWKAFAPAFAGKMAIEAMDRAMRGEGSP
APIWEGEDGVIAWLLSGKDHVYHVPLPEHGEPKLGILETYTKEHSAEYQSQAPIDLARRMKPLVDAAGGTEHIAEIVLRT
SHHTHYVIGTGANDPQKMDPQASRETLDHSIMYIFAVALQDGVWHHEFSYTRKRSTRPETVELWHKIRTVEDPEWTRRYH
SDDPAKKAFGAKAVITMADGTLIEDELAVADAHPLGARPFARENYIEKFRTLAQGIVIDSEQERFLHAVQSLPDLDDLDQ
LNIEVDISNQAATKAGLL

Sequences:

>Translated_498_residues
MRIHDVYTHLSADNFPKAEHLAWKISELATDPVEVTPDVSEMIINRIIDNAAVSAASVLRRPVTVARQQAQSHPREKGGK
VFGISGSYSPEWAAFANGVAVRELDFHDTFLAAEYSHPGDNIPPLLAVAQAQRSSGRDLIRGIATAYEVQVELVKGICLH
EHKIDHVAHLGPSAAAGLGTLLHVDEETIYQAIGQALHTTTATRQSRKGEISSWKAFAPAFAGKMAIEAMDRAMRGEGSP
APIWEGEDGVIAWLLSGKDHVYHVPLPEHGEPKLGILETYTKEHSAEYQSQAPIDLARRMKPLVDAAGGTEHIAEIVLRT
SHHTHYVIGTGANDPQKMDPQASRETLDHSIMYIFAVALQDGVWHHEFSYTRKRSTRPETVELWHKIRTVEDPEWTRRYH
SDDPAKKAFGAKAVITMADGTLIEDELAVADAHPLGARPFARENYIEKFRTLAQGIVIDSEQERFLHAVQSLPDLDDLDQ
LNIEVDISNQAATKAGLL
>Mature_498_residues
MRIHDVYTHLSADNFPKAEHLAWKISELATDPVEVTPDVSEMIINRIIDNAAVSAASVLRRPVTVARQQAQSHPREKGGK
VFGISGSYSPEWAAFANGVAVRELDFHDTFLAAEYSHPGDNIPPLLAVAQAQRSSGRDLIRGIATAYEVQVELVKGICLH
EHKIDHVAHLGPSAAAGLGTLLHVDEETIYQAIGQALHTTTATRQSRKGEISSWKAFAPAFAGKMAIEAMDRAMRGEGSP
APIWEGEDGVIAWLLSGKDHVYHVPLPEHGEPKLGILETYTKEHSAEYQSQAPIDLARRMKPLVDAAGGTEHIAEIVLRT
SHHTHYVIGTGANDPQKMDPQASRETLDHSIMYIFAVALQDGVWHHEFSYTRKRSTRPETVELWHKIRTVEDPEWTRRYH
SDDPAKKAFGAKAVITMADGTLIEDELAVADAHPLGARPFARENYIEKFRTLAQGIVIDSEQERFLHAVQSLPDLDDLDQ
LNIEVDISNQAATKAGLL

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=377, Percent_Identity=26.790450928382, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325258, Length=439, Percent_Identity=25.2847380410023, Blast_Score=98, Evalue=3e-21,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 54965; Mature: 54965

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIHDVYTHLSADNFPKAEHLAWKISELATDPVEVTPDVSEMIINRIIDNAAVSAASVLR
CCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RPVTVARQQAQSHPREKGGKVFGISGSYSPEWAAFANGVAVRELDFHDTFLAAEYSHPGD
HHHHHHHHHHHCCHHHHCCEEEEECCCCCCCHHHHHCCEEEEEECCCCCEEEEECCCCCC
NIPPLLAVAQAQRSSGRDLIRGIATAYEVQVELVKGICLHEHKIDHVAHLGPSAAAGLGT
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHH
LLHVDEETIYQAIGQALHTTTATRQSRKGEISSWKAFAPAFAGKMAIEAMDRAMRGEGSP
EEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
APIWEGEDGVIAWLLSGKDHVYHVPLPEHGEPKLGILETYTKEHSAEYQSQAPIDLARRM
CCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
KPLVDAAGGTEHIAEIVLRTSHHTHYVIGTGANDPQKMDPQASRETLDHSIMYIFAVALQ
HHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DGVWHHEFSYTRKRSTRPETVELWHKIRTVEDPEWTRRYHSDDPAKKAFGAKAVITMADG
CCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHCCCEEEEEECCC
TLIEDELAVADAHPLGARPFARENYIEKFRTLAQGIVIDSEQERFLHAVQSLPDLDDLDQ
CEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCHHHHHHHHHHCCCCCCCCCC
LNIEVDISNQAATKAGLL
EEEEEEECCCHHHHHCCC
>Mature Secondary Structure
MRIHDVYTHLSADNFPKAEHLAWKISELATDPVEVTPDVSEMIINRIIDNAAVSAASVLR
CCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RPVTVARQQAQSHPREKGGKVFGISGSYSPEWAAFANGVAVRELDFHDTFLAAEYSHPGD
HHHHHHHHHHHCCHHHHCCEEEEECCCCCCCHHHHHCCEEEEEECCCCCEEEEECCCCCC
NIPPLLAVAQAQRSSGRDLIRGIATAYEVQVELVKGICLHEHKIDHVAHLGPSAAAGLGT
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHH
LLHVDEETIYQAIGQALHTTTATRQSRKGEISSWKAFAPAFAGKMAIEAMDRAMRGEGSP
EEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
APIWEGEDGVIAWLLSGKDHVYHVPLPEHGEPKLGILETYTKEHSAEYQSQAPIDLARRM
CCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
KPLVDAAGGTEHIAEIVLRTSHHTHYVIGTGANDPQKMDPQASRETLDHSIMYIFAVALQ
HHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DGVWHHEFSYTRKRSTRPETVELWHKIRTVEDPEWTRRYHSDDPAKKAFGAKAVITMADG
CCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHCCCEEEEEECCC
TLIEDELAVADAHPLGARPFARENYIEKFRTLAQGIVIDSEQERFLHAVQSLPDLDDLDQ
CEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCHHHHHHHHHHCCCCCCCCCC
LNIEVDISNQAATKAGLL
EEEEEEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626 [H]