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The map label for this gene is purR [H]

Identifier: 145294157

GI number: 145294157

Start: 134356

End: 135357

Strand: Reverse

Name: purR [H]

Synonym: cgR_0115

Alternate gene names: 145294157

Gene position: 135357-134356 (Counterclockwise)

Preceding gene: 145294160

Following gene: 145294156

Centisome position: 4.08

GC content: 50.7

Gene sequence:

>1002_bases
GTGCCGACCAGCAGAGATGTGGCACGGGCTGCCGGAGTGTCGCAGGCCACAGTGTCTCGGGCGTTAAATCGCCCAGAAAC
TGTGTCGCAGAAAACCCGTGAGCGGGTTCAAGCTGCAGTCCAAACTCTCGGATACCACCCTCATTCCGGTGCTATCGCAA
TGAAAACTCAGCGCACCAAAACAATCGGAGTCGTTGTCTCTGAGTTATCCAACCCCTTTTTCCAAGAGATCTTCGAAACC
CTCAGTCAGGAATTTGCCAAGGCGGGATCTCGGGTCATCGTGTGGGATTCAGCTCTACATGAACGCGATGCTATCCGGGC
ACTCCAGGAAAGAAGCGTTGACGGGGTGATTATGTCTGCTTGGGATGAAGACGCCCAAGAAATGCGAGATATCCTTGAGA
CAGGACTTCCCGTCGTTTTGCTCAACCGCACTGCCCCTAAGGGTTCTTTCGATTCGGTTACTAGCGATAATCACAACGGT
GGCCAGTTGGTGGCAGATTATCTTCATCAGCATGGGAAACACCATCTAGTGTTCATTCAGGGCAACCCGCACACCAGCAC
TTCTATGGAACGATTAAACGGTTTTCGAGCAGGCCTACACGCACTAAATCAACCATCACCTACTGTGCTCAACGGTGAAT
TCTCCTTTGAAAAATCATTTGCTGTTACCCGTGATTTCCTCGATCAACATGGTGCCCCCGATGCCTTTTTCTGCGCCAAT
GATTATATGGCTTTCGGTGTTCTCAATGCCGTCAAAGCTGCCGGTATGTCCGTTCCGGAAGATGTCTGGGTTATCGGTTA
CGACAACGTAAGCCAATCCGATTGGCCCATCTTGGATCTCACCACAGTCCGTCAAGGCAGCCGTCAGATGGCGCAATTAG
GTGCCCAGAAACTTCTAAACCGAATAGAAAATCCCACCACCCCAGCCGTCAAAACAATTCTTCCGGTCCAACTAGAAATC
CGTGGCTCCAGCGAAAATGCTCCATTTAATCATGAAAACTAA

Upstream 100 bases:

>100_bases
AAGGGGTTTTGTTACCCCCGGTGCGATGACCCCAGAAGAGGGCAGCTGTTCATTAGTTTCGATAAGCTCAGTAGTCCATA
CGTTGAGAGAGGAAAAGATA

Downstream 100 bases:

>100_bases
CTGTTTTAGGTCATCCTGGGGCGATTTATTACCCCACAATGCGGCCCAAATGAAGAAGAGGCCTATAGGCGAATATATGT
TCTGTAGACCTCTTCAACTT

Product: hypothetical protein

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MPTSRDVARAAGVSQATVSRALNRPETVSQKTRERVQAAVQTLGYHPHSGAIAMKTQRTKTIGVVVSELSNPFFQEIFET
LSQEFAKAGSRVIVWDSALHERDAIRALQERSVDGVIMSAWDEDAQEMRDILETGLPVVLLNRTAPKGSFDSVTSDNHNG
GQLVADYLHQHGKHHLVFIQGNPHTSTSMERLNGFRAGLHALNQPSPTVLNGEFSFEKSFAVTRDFLDQHGAPDAFFCAN
DYMAFGVLNAVKAAGMSVPEDVWVIGYDNVSQSDWPILDLTTVRQGSRQMAQLGAQKLLNRIENPTTPAVKTILPVQLEI
RGSSENAPFNHEN

Sequences:

>Translated_333_residues
MPTSRDVARAAGVSQATVSRALNRPETVSQKTRERVQAAVQTLGYHPHSGAIAMKTQRTKTIGVVVSELSNPFFQEIFET
LSQEFAKAGSRVIVWDSALHERDAIRALQERSVDGVIMSAWDEDAQEMRDILETGLPVVLLNRTAPKGSFDSVTSDNHNG
GQLVADYLHQHGKHHLVFIQGNPHTSTSMERLNGFRAGLHALNQPSPTVLNGEFSFEKSFAVTRDFLDQHGAPDAFFCAN
DYMAFGVLNAVKAAGMSVPEDVWVIGYDNVSQSDWPILDLTTVRQGSRQMAQLGAQKLLNRIENPTTPAVKTILPVQLEI
RGSSENAPFNHEN
>Mature_332_residues
PTSRDVARAAGVSQATVSRALNRPETVSQKTRERVQAAVQTLGYHPHSGAIAMKTQRTKTIGVVVSELSNPFFQEIFETL
SQEFAKAGSRVIVWDSALHERDAIRALQERSVDGVIMSAWDEDAQEMRDILETGLPVVLLNRTAPKGSFDSVTSDNHNGG
QLVADYLHQHGKHHLVFIQGNPHTSTSMERLNGFRAGLHALNQPSPTVLNGEFSFEKSFAVTRDFLDQHGAPDAFFCAND
YMAFGVLNAVKAAGMSVPEDVWVIGYDNVSQSDWPILDLTTVRQGSRQMAQLGAQKLLNRIENPTTPAVKTILPVQLEIR
GSSENAPFNHEN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=31.3253012048193, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=30.7692307692308, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=30.1775147928994, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788474, Length=304, Percent_Identity=30.9210526315789, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789068, Length=300, Percent_Identity=30, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789202, Length=340, Percent_Identity=28.5294117647059, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786540, Length=334, Percent_Identity=29.940119760479, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787580, Length=281, Percent_Identity=25.6227758007117, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787906, Length=333, Percent_Identity=26.1261261261261, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994940, Length=308, Percent_Identity=25, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786268, Length=320, Percent_Identity=23.75, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790192, Length=245, Percent_Identity=29.7959183673469, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790715, Length=307, Percent_Identity=23.1270358306189, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790689, Length=249, Percent_Identity=28.5140562248996, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789456, Length=342, Percent_Identity=23.6842105263158, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36613; Mature: 36482

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSRDVARAAGVSQATVSRALNRPETVSQKTRERVQAAVQTLGYHPHSGAIAMKTQRTK
CCCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHH
TIGVVVSELSNPFFQEIFETLSQEFAKAGSRVIVWDSALHERDAIRALQERSVDGVIMSA
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEC
WDEDAQEMRDILETGLPVVLLNRTAPKGSFDSVTSDNHNGGQLVADYLHQHGKHHLVFIQ
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEE
GNPHTSTSMERLNGFRAGLHALNQPSPTVLNGEFSFEKSFAVTRDFLDQHGAPDAFFCAN
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCEEEECC
DYMAFGVLNAVKAAGMSVPEDVWVIGYDNVSQSDWPILDLTTVRQGSRQMAQLGAQKLLN
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
RIENPTTPAVKTILPVQLEIRGSSENAPFNHEN
HHCCCCCCHHHHEEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
PTSRDVARAAGVSQATVSRALNRPETVSQKTRERVQAAVQTLGYHPHSGAIAMKTQRTK
CCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHH
TIGVVVSELSNPFFQEIFETLSQEFAKAGSRVIVWDSALHERDAIRALQERSVDGVIMSA
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEC
WDEDAQEMRDILETGLPVVLLNRTAPKGSFDSVTSDNHNGGQLVADYLHQHGKHHLVFIQ
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEE
GNPHTSTSMERLNGFRAGLHALNQPSPTVLNGEFSFEKSFAVTRDFLDQHGAPDAFFCAN
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCEEEECC
DYMAFGVLNAVKAAGMSVPEDVWVIGYDNVSQSDWPILDLTTVRQGSRQMAQLGAQKLLN
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
RIENPTTPAVKTILPVQLEIRGSSENAPFNHEN
HHCCCCCCHHHHEEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA