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The map label for this gene is ytwF [H]

Identifier: 145294115

GI number: 145294115

Start: 84991

End: 85284

Strand: Reverse

Name: ytwF [H]

Synonym: cgR_0073

Alternate gene names: 145294115

Gene position: 85284-84991 (Counterclockwise)

Preceding gene: 145294116

Following gene: 145294114

Centisome position: 2.57

GC content: 48.64

Gene sequence:

>294_bases
ATGCCTATCACCATTACTGAAACTAACAAGCGCCGAAACTTGGATCAGATCCTGGATGTGCGCGAAAATTTTGAAATCAC
CGAAGGTATGATCCCCGAAGCGATACATATTCCCATGGGGGACCTCAGCTTTCGCTTCAAAGAACTAGATCAGAATCGGC
CGGTCATTGTGGTGTGCCGTAGCGGTAACCGCAGTACACAGGTGGCAAATGCACTTAAACAGGCGGGATATACCGCGTAC
TCTATGACTGGCGGAATGAGTGCGTGGTACCAAAACAATCTGCCGATTAAGTAG

Upstream 100 bases:

>100_bases
CATGGGCAAAGCTCTGTCCGCCTTGCCGTGGAATAAAACTAAGCCAGTTCCTACAAAAGCTGAGACCTTGAGCAAGCTTC
CAAGCCCTAAGGAGAACTAA

Downstream 100 bases:

>100_bases
GAAGTCCCCATGACCGCCACTTTGATAATCACGTTGTTTCTGTCACTTTTTATCGGCATTTCGCTAGGACTGTTGGGCGG
TGGTGGTTCGATCCTCACTG

Product: hypothetical protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 97; Mature: 96

Protein sequence:

>97_residues
MPITITETNKRRNLDQILDVRENFEITEGMIPEAIHIPMGDLSFRFKELDQNRPVIVVCRSGNRSTQVANALKQAGYTAY
SMTGGMSAWYQNNLPIK

Sequences:

>Translated_97_residues
MPITITETNKRRNLDQILDVRENFEITEGMIPEAIHIPMGDLSFRFKELDQNRPVIVVCRSGNRSTQVANALKQAGYTAY
SMTGGMSAWYQNNLPIK
>Mature_96_residues
PITITETNKRRNLDQILDVRENFEITEGMIPEAIHIPMGDLSFRFKELDQNRPVIVVCRSGNRSTQVANALKQAGYTAYS
MTGGMSAWYQNNLPIK

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11017; Mature: 10886

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITITETNKRRNLDQILDVRENFEITEGMIPEAIHIPMGDLSFRFKELDQNRPVIVVCR
CCEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCEEEEEC
SGNRSTQVANALKQAGYTAYSMTGGMSAWYQNNLPIK
CCCCCHHHHHHHHHCCCEEEECCCCCCEEECCCCCCC
>Mature Secondary Structure 
PITITETNKRRNLDQILDVRENFEITEGMIPEAIHIPMGDLSFRFKELDQNRPVIVVCR
CEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCCCEEEEEC
SGNRSTQVANALKQAGYTAYSMTGGMSAWYQNNLPIK
CCCCCHHHHHHHHHCCCEEEECCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]