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The map label for this gene is yhcR [H]

Identifier: 145294068

GI number: 145294068

Start: 27029

End: 28984

Strand: Direct

Name: yhcR [H]

Synonym: cgR_0026

Alternate gene names: 145294068

Gene position: 27029-28984 (Clockwise)

Preceding gene: 145294067

Following gene: 145294069

Centisome position: 0.82

GC content: 54.24

Gene sequence:

>1956_bases
GTGTTTCTATCCGCATACCGCGCACCGATTGCCGCAGGCACCGCTGAAGCCGCTGCTCTAGCTGGAGTCTCTGTTGCCCA
GGCGCAGGAAAATATCTCACAGTTCAACATCGCAAATATCACCGACTTCCACGGCTACATCTCTGCAACCGCAGCTCACC
CAGGTGCAGCAATGCTGAAATGTGCAGTTGATGAAGCAGCTGGTGGACGCGCCCAGGCATTCGTGTCTTCCGGCGACAAC
ATTGGTGGCAGTCCATTCCAATCCTTCATCCTTGGTGATGAACCCACCTTGGAAGCACTGAACCAAATGGGCCTTGATTA
CTCAGCAGTGGGCAACCACGAATTTGATAAGGGCTACGCAGATTTAAGTGGTCGAGTCGCTGATATTGCAGATTTTGATT
ACCTCGGCGCAAACGTTGATGGCGAAAATCCAGATCTTGCACCTTATGGAATTTCTGAACTCGACGGCGTGAAGGTGGCT
TTTGTGGGCACCGTATCCCAAGAAACTCCGATGCTGGTCAATTCCGAAGGCATTGAGGGAATCACCTTCACCGACCCACT
GGAAGCAACCAACCGTTTAGCTGATCAGCTCGTGGAAAGCGGCGAAGCAGATGTTGTCGTTGCTCTTTACCACGAAGGTA
TCACCGGTACCGAAGCATGGTCAGAAAATATCGACGCTGTTTTTGCAGGTCACACCCACCAAGTCCGTGACCTTGGAACC
GACAACGGACCGTTGATCATGCAGTGTGGCAACTACGGACATGCACTCGCCGATGTAGATTTCAGCTTCAACCACGACAC
CGGTGAGCTCACCGTGGATGATGCTCGCATGCTCGGAGTTGCAGACATCAACGCATGTGGAAACCCAGATGGAACCCTCG
CGGGAATCGTTGCCCAAGCGGAACTTGATGCTGGTGAAGCCGGCAAGGAAGTGGTGGCAACCATCGACGGTGACTTTCTC
CGCGCCACCGATGAGGGAGCAGAATCTGGTTCTAACTACGGAGCGGAATCTCAGCTCGTCAACATGATTGCCAGTGCAGT
GCGTTGGTCCATGTCTACCAACACCGCCACCACCGCAGATATCGGTCTCATGAACGCCGGTGGACTTCGCGCAGACCTAT
TTGGCGGCGAAGTTACCTATGCCGAAGCGTTTGAAATCCAGCCATTCTCCGGTGAAGATTCCTTTGTCACCCTCAAGGGA
TCAGACTTCAAAGATGCCCTCGAGCAGCAGTGGAAAGAAGATTCAGCACGTCCAGTGGCAGCACTTGGTGTCTCCGACAA
TGTTTCCTACACCTATGACATCAAACGCCCAATCGGTGACCGCGTCACTTCCGTGACCATTGATGATGCCCCACTTGGTC
CTGAATGCGACTACGTTGTGGCAGCCTCCCTGTACCTTCTGTCCGGCAATGAAGGCATGACCGCACTGACCCGCGGAACC
GCACCTGCGCAAACCGGCATCGTGGATGTGCAGTCCACCATCGGCTACTTGGCCAACAACGTTGTCACCCCACGTACTGG
TCAGGCCCAGATCTCCATCACCCCATCCGGTGACTTCAATGCTGGCGAGACCGTTACCCTTGACCTGGCTGGACTCCGCT
ACACCCAAGGCGACACCGCAAAGGAAGTAACCGTCAGCCTTGGTGATGCAATTGATTCAGCACCAATTGATCCTCAGCTC
GGCGCTGCTGGCTTTGGTGAAGCTGGAATCGCAACTGTGAGCCTGGAGATTCCTGCAGCTCTTTCAGGTACTCAAAACCT
CGTTGTCACCACCGATGCCGGCACCCGTATTTCCATGCCAGTTGAGGTTGTTGGCGCAGAACAGCCAGCACCGCAGCCAG
CAGGTTCTTCAGTTCTCGGAACTGGAATTCTTAGTGGAATCCTCGGAATGGTCGGTCTGGGGAACTGGATTGACCCAAGC
TTCATTCAACACATCCAGCAGCAGCTCTTCGCATAG

Upstream 100 bases:

>100_bases
CACCCTGCGCATGAACATTTGGTGACCACCCAAGCGGTACTTTTCCTGCACCGGTAGACACTTAAGTGTCTGAAAATAAT
ACCTTTAAACAGGAGAAGTA

Downstream 100 bases:

>100_bases
ATTTAGCCAAAAACTATGCCTTTAGCTGCGGATTTCTTTAGAAACATGGTCTAGGTGTAAAATCTAAAGAGCAAGTTTTA
AAGCTTGTTCTTTAGTTCGG

Product: hypothetical protein

Products: Phosphate; Uridine [C]

Alternate protein names: NA

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MFLSAYRAPIAAGTAEAAALAGVSVAQAQENISQFNIANITDFHGYISATAAHPGAAMLKCAVDEAAGGRAQAFVSSGDN
IGGSPFQSFILGDEPTLEALNQMGLDYSAVGNHEFDKGYADLSGRVADIADFDYLGANVDGENPDLAPYGISELDGVKVA
FVGTVSQETPMLVNSEGIEGITFTDPLEATNRLADQLVESGEADVVVALYHEGITGTEAWSENIDAVFAGHTHQVRDLGT
DNGPLIMQCGNYGHALADVDFSFNHDTGELTVDDARMLGVADINACGNPDGTLAGIVAQAELDAGEAGKEVVATIDGDFL
RATDEGAESGSNYGAESQLVNMIASAVRWSMSTNTATTADIGLMNAGGLRADLFGGEVTYAEAFEIQPFSGEDSFVTLKG
SDFKDALEQQWKEDSARPVAALGVSDNVSYTYDIKRPIGDRVTSVTIDDAPLGPECDYVVAASLYLLSGNEGMTALTRGT
APAQTGIVDVQSTIGYLANNVVTPRTGQAQISITPSGDFNAGETVTLDLAGLRYTQGDTAKEVTVSLGDAIDSAPIDPQL
GAAGFGEAGIATVSLEIPAALSGTQNLVVTTDAGTRISMPVEVVGAEQPAPQPAGSSVLGTGILSGILGMVGLGNWIDPS
FIQHIQQQLFA

Sequences:

>Translated_651_residues
MFLSAYRAPIAAGTAEAAALAGVSVAQAQENISQFNIANITDFHGYISATAAHPGAAMLKCAVDEAAGGRAQAFVSSGDN
IGGSPFQSFILGDEPTLEALNQMGLDYSAVGNHEFDKGYADLSGRVADIADFDYLGANVDGENPDLAPYGISELDGVKVA
FVGTVSQETPMLVNSEGIEGITFTDPLEATNRLADQLVESGEADVVVALYHEGITGTEAWSENIDAVFAGHTHQVRDLGT
DNGPLIMQCGNYGHALADVDFSFNHDTGELTVDDARMLGVADINACGNPDGTLAGIVAQAELDAGEAGKEVVATIDGDFL
RATDEGAESGSNYGAESQLVNMIASAVRWSMSTNTATTADIGLMNAGGLRADLFGGEVTYAEAFEIQPFSGEDSFVTLKG
SDFKDALEQQWKEDSARPVAALGVSDNVSYTYDIKRPIGDRVTSVTIDDAPLGPECDYVVAASLYLLSGNEGMTALTRGT
APAQTGIVDVQSTIGYLANNVVTPRTGQAQISITPSGDFNAGETVTLDLAGLRYTQGDTAKEVTVSLGDAIDSAPIDPQL
GAAGFGEAGIATVSLEIPAALSGTQNLVVTTDAGTRISMPVEVVGAEQPAPQPAGSSVLGTGILSGILGMVGLGNWIDPS
FIQHIQQQLFA
>Mature_651_residues
MFLSAYRAPIAAGTAEAAALAGVSVAQAQENISQFNIANITDFHGYISATAAHPGAAMLKCAVDEAAGGRAQAFVSSGDN
IGGSPFQSFILGDEPTLEALNQMGLDYSAVGNHEFDKGYADLSGRVADIADFDYLGANVDGENPDLAPYGISELDGVKVA
FVGTVSQETPMLVNSEGIEGITFTDPLEATNRLADQLVESGEADVVVALYHEGITGTEAWSENIDAVFAGHTHQVRDLGT
DNGPLIMQCGNYGHALADVDFSFNHDTGELTVDDARMLGVADINACGNPDGTLAGIVAQAELDAGEAGKEVVATIDGDFL
RATDEGAESGSNYGAESQLVNMIASAVRWSMSTNTATTADIGLMNAGGLRADLFGGEVTYAEAFEIQPFSGEDSFVTLKG
SDFKDALEQQWKEDSARPVAALGVSDNVSYTYDIKRPIGDRVTSVTIDDAPLGPECDYVVAASLYLLSGNEGMTALTRGT
APAQTGIVDVQSTIGYLANNVVTPRTGQAQISITPSGDFNAGETVTLDLAGLRYTQGDTAKEVTVSLGDAIDSAPIDPQL
GAAGFGEAGIATVSLEIPAALSGTQNLVVTTDAGTRISMPVEVVGAEQPAPQPAGSSVLGTGILSGILGMVGLGNWIDPS
FIQHIQQQLFA

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786687, Length=523, Percent_Identity=21.6061185468451, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI1790658, Length=529, Percent_Identity=23.062381852552, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24641187, Length=478, Percent_Identity=25.7322175732218, Blast_Score=86, Evalue=1e-16,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.6.1.45; 3.1.3.5 [C]

Molecular weight: Translated: 67262; Mature: 67262

Theoretical pI: Translated: 3.78; Mature: 3.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLSAYRAPIAAGTAEAAALAGVSVAQAQENISQFNIANITDFHGYISATAAHPGAAMLK
CCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCHHEEE
CAVDEAAGGRAQAFVSSGDNIGGSPFQSFILGDEPTLEALNQMGLDYSAVGNHEFDKGYA
EEHHHCCCCCEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCCCCHH
DLSGRVADIADFDYLGANVDGENPDLAPYGISELDGVKVAFVGTVSQETPMLVNSEGIEG
HCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCC
ITFTDPLEATNRLADQLVESGEADVVVALYHEGITGTEAWSENIDAVFAGHTHQVRDLGT
EEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCCCEEEECCCEEHHHCCC
DNGPLIMQCGNYGHALADVDFSFNHDTGELTVDDARMLGVADINACGNPDGTLAGIVAQA
CCCCEEEEECCCCCEEEEEEEEECCCCCCEEECCCEEEEEEECCCCCCCCCCEEEEEEEE
ELDAGEAGKEVVATIDGDFLRATDEGAESGSNYGAESQLVNMIASAVRWSMSTNTATTAD
ECCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEE
IGLMNAGGLRADLFGGEVTYAEAFEIQPFSGEDSFVTLKGSDFKDALEQQWKEDSARPVA
EEEECCCCCEEEECCCEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEE
ALGVSDNVSYTYDIKRPIGDRVTSVTIDDAPLGPECDYVVAASLYLLSGNEGMTALTRGT
EEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEECCCCCEEEECCC
APAQTGIVDVQSTIGYLANNVVTPRTGQAQISITPSGDFNAGETVTLDLAGLRYTQGDTA
CCCCCCEEEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCEEECCCCCC
KEVTVSLGDAIDSAPIDPQLGAAGFGEAGIATVSLEIPAALSGTQNLVVTTDAGTRISMP
CEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCEEECC
VEVVGAEQPAPQPAGSSVLGTGILSGILGMVGLGNWIDPSFIQHIQQQLFA
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFLSAYRAPIAAGTAEAAALAGVSVAQAQENISQFNIANITDFHGYISATAAHPGAAMLK
CCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCHHEEE
CAVDEAAGGRAQAFVSSGDNIGGSPFQSFILGDEPTLEALNQMGLDYSAVGNHEFDKGYA
EEHHHCCCCCEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCCCCHH
DLSGRVADIADFDYLGANVDGENPDLAPYGISELDGVKVAFVGTVSQETPMLVNSEGIEG
HCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCC
ITFTDPLEATNRLADQLVESGEADVVVALYHEGITGTEAWSENIDAVFAGHTHQVRDLGT
EEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCCCEEEECCCEEHHHCCC
DNGPLIMQCGNYGHALADVDFSFNHDTGELTVDDARMLGVADINACGNPDGTLAGIVAQA
CCCCEEEEECCCCCEEEEEEEEECCCCCCEEECCCEEEEEEECCCCCCCCCCEEEEEEEE
ELDAGEAGKEVVATIDGDFLRATDEGAESGSNYGAESQLVNMIASAVRWSMSTNTATTAD
ECCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEE
IGLMNAGGLRADLFGGEVTYAEAFEIQPFSGEDSFVTLKGSDFKDALEQQWKEDSARPVA
EEEECCCCCEEEECCCEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEE
ALGVSDNVSYTYDIKRPIGDRVTSVTIDDAPLGPECDYVVAASLYLLSGNEGMTALTRGT
EEECCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEECCCCCEEEECCC
APAQTGIVDVQSTIGYLANNVVTPRTGQAQISITPSGDFNAGETVTLDLAGLRYTQGDTA
CCCCCCEEEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCEEECCCCCC
KEVTVSLGDAIDSAPIDPQLGAAGFGEAGIATVSLEIPAALSGTQNLVVTTDAGTRISMP
CEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCEEECC
VEVVGAEQPAPQPAGSSVLGTGILSGILGMVGLGNWIDPSFIQHIQQQLFA
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UMP [C]

Specific reaction: H2O + UMP --> Phosphate + Uridine [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Inhibitor from Salmonella typhimurium cytoplasm [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]