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The map label for this gene is purR [H]

Identifier: 138896844

GI number: 138896844

Start: 3314629

End: 3315642

Strand: Direct

Name: purR [H]

Synonym: GTNG_3207

Alternate gene names: 138896844

Gene position: 3314629-3315642 (Clockwise)

Preceding gene: 138896833

Following gene: 138896846

Centisome position: 93.36

GC content: 49.7

Gene sequence:

>1014_bases
ATGAAAAAACGGGTCACCATGCAAGACATCGCTGATCGGCTCAACATTTCTAAAAACTCCGTCTCTCAAGCGCTGCGAGG
CAAAGAAGGGGTGAGCGAAGAAACGAGGCAGCTTGTAAAGCGTGTCGCGAAAGAAATGGGGTATGAATATCCCTCCTCTC
GCTCGAAACATCGCGAGGAGCCGCCGAAGCAAATCGCCCTCATCGCTTCAGACCGCGCATTTTCGCTGAAATTTTTCGGC
GAAATTTACTTGAGCATCGAACAGGAAGCGCTATCACATGGCATGAAGCTACACATTCAGTCGGTCAACGAGCAACAAAA
ACAACAACGTCTGTTGCCTTCGTTCATCGAGGAAAAGCTCGTTGACGGCGTCATCATTCTTTCCCATATCAGCACCGAAT
ACATCCAAAAAGTGGTCTCAACCGGCATCCCGACCGTCCTTGTCGATCACCATCATCCGAACATTTCCGCTGATGCGGTG
CTGACAAACAATCGATTCGGCGCCTACATCGCTGTCCAGCATTTGTTGGAGCTCAACCATACCGATATTGCGTTTGTCGG
CGACATTCACTATTCACCGAGCTATCAAGAACGGTATGAAGGGTATTTATTGGCGTTGAAAGATTACGGGGTGAAACCGA
ACGAGGAATGGATATTTTGCCACGCACAAGAGGACGAAACAGCGATCGCCAACTACATACGAGAGCTAAAGCAGCAACCA
ACCGCCTGGTTTTGCGTCAATGACGGATTAGGCTTTTTCGTCAGCACCAGCCTCCAACAATACGGCCTGAACGTACCTGG
CGATGTCTCCGTCTGTAGCTTTGATAACGGACAACTATCGCAAATCGCCACGCCGAAAATTACAACCGTCGACATCGATC
TGAAGCGATACGGCAAACGAGCCGTGGAATTGTTACGGTGGCGGTGGGACAATCCGGACGAACCATTTCAGGAAGTGTTA
TTATCGACGAAACTCATCAAACGGGAATCGACAGCGGCGAAACCAAAGCGTTAG

Upstream 100 bases:

>100_bases
CCGACCTATAATCAGGGATAGGAAAGCGATGACAATCGTCATCCTTATCGCACTTCAAATAGCCGAAAAGGCAAAAAGAG
TGAACAGGTGGAGATAGCGA

Downstream 100 bases:

>100_bases
GGCGGGGCTCTCTTTGCCCTGCCTGTTTGCCGAACGTTAGCGATTCACTCCTCTTCATTTGCAGGCGTCGGATTTCATCC
ATTCACAGGCAATCCCGTCA

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKKRVTMQDIADRLNISKNSVSQALRGKEGVSEETRQLVKRVAKEMGYEYPSSRSKHREEPPKQIALIASDRAFSLKFFG
EIYLSIEQEALSHGMKLHIQSVNEQQKQQRLLPSFIEEKLVDGVIILSHISTEYIQKVVSTGIPTVLVDHHHPNISADAV
LTNNRFGAYIAVQHLLELNHTDIAFVGDIHYSPSYQERYEGYLLALKDYGVKPNEEWIFCHAQEDETAIANYIRELKQQP
TAWFCVNDGLGFFVSTSLQQYGLNVPGDVSVCSFDNGQLSQIATPKITTVDIDLKRYGKRAVELLRWRWDNPDEPFQEVL
LSTKLIKRESTAAKPKR

Sequences:

>Translated_337_residues
MKKRVTMQDIADRLNISKNSVSQALRGKEGVSEETRQLVKRVAKEMGYEYPSSRSKHREEPPKQIALIASDRAFSLKFFG
EIYLSIEQEALSHGMKLHIQSVNEQQKQQRLLPSFIEEKLVDGVIILSHISTEYIQKVVSTGIPTVLVDHHHPNISADAV
LTNNRFGAYIAVQHLLELNHTDIAFVGDIHYSPSYQERYEGYLLALKDYGVKPNEEWIFCHAQEDETAIANYIRELKQQP
TAWFCVNDGLGFFVSTSLQQYGLNVPGDVSVCSFDNGQLSQIATPKITTVDIDLKRYGKRAVELLRWRWDNPDEPFQEVL
LSTKLIKRESTAAKPKR
>Mature_337_residues
MKKRVTMQDIADRLNISKNSVSQALRGKEGVSEETRQLVKRVAKEMGYEYPSSRSKHREEPPKQIALIASDRAFSLKFFG
EIYLSIEQEALSHGMKLHIQSVNEQQKQQRLLPSFIEEKLVDGVIILSHISTEYIQKVVSTGIPTVLVDHHHPNISADAV
LTNNRFGAYIAVQHLLELNHTDIAFVGDIHYSPSYQERYEGYLLALKDYGVKPNEEWIFCHAQEDETAIANYIRELKQQP
TAWFCVNDGLGFFVSTSLQQYGLNVPGDVSVCSFDNGQLSQIATPKITTVDIDLKRYGKRAVELLRWRWDNPDEPFQEVL
LSTKLIKRESTAAKPKR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=337, Percent_Identity=29.9703264094956, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=27.9411764705882, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789202, Length=331, Percent_Identity=24.1691842900302, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1790194, Length=334, Percent_Identity=26.3473053892216, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=24.251497005988, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789068, Length=339, Percent_Identity=24.4837758112094, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI48994940, Length=324, Percent_Identity=25.9259259259259, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787580, Length=311, Percent_Identity=24.7588424437299, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787906, Length=251, Percent_Identity=24.7011952191235, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786540, Length=342, Percent_Identity=23.3918128654971, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790689, Length=347, Percent_Identity=22.478386167147, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38446; Mature: 38446

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRVTMQDIADRLNISKNSVSQALRGKEGVSEETRQLVKRVAKEMGYEYPSSRSKHREE
CCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCC
PPKQIALIASDRAFSLKFFGEIYLSIEQEALSHGMKLHIQSVNEQQKQQRLLPSFIEEKL
CCHHEEEEECCCEEEEEEEEHHHEEEEHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHH
VDGVIILSHISTEYIQKVVSTGIPTVLVDHHHPNISADAVLTNNRFGAYIAVQHLLELNH
HCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
TDIAFVGDIHYSPSYQERYEGYLLALKDYGVKPNEEWIFCHAQEDETAIANYIRELKQQP
CCEEEEEEECCCCCHHHHCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCC
TAWFCVNDGLGFFVSTSLQQYGLNVPGDVSVCSFDNGQLSQIATPKITTVDIDLKRYGKR
CEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHCCCCCEEEEEEHHHHHHHH
AVELLRWRWDNPDEPFQEVLLSTKLIKRESTAAKPKR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKKRVTMQDIADRLNISKNSVSQALRGKEGVSEETRQLVKRVAKEMGYEYPSSRSKHREE
CCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCC
PPKQIALIASDRAFSLKFFGEIYLSIEQEALSHGMKLHIQSVNEQQKQQRLLPSFIEEKL
CCHHEEEEECCCEEEEEEEEHHHEEEEHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHH
VDGVIILSHISTEYIQKVVSTGIPTVLVDHHHPNISADAVLTNNRFGAYIAVQHLLELNH
HCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHCCC
TDIAFVGDIHYSPSYQERYEGYLLALKDYGVKPNEEWIFCHAQEDETAIANYIRELKQQP
CCEEEEEEECCCCCHHHHCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCC
TAWFCVNDGLGFFVSTSLQQYGLNVPGDVSVCSFDNGQLSQIATPKITTVDIDLKRYGKR
CEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHCCCCCEEEEEEHHHHHHHH
AVELLRWRWDNPDEPFQEVLLSTKLIKRESTAAKPKR
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA