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The map label for this gene is ykfC [H]

Identifier: 138896819

GI number: 138896819

Start: 3283985

End: 3284992

Strand: Direct

Name: ykfC [H]

Synonym: GTNG_3182

Alternate gene names: 138896819

Gene position: 3283985-3284992 (Clockwise)

Preceding gene: 138896818

Following gene: 138896829

Centisome position: 92.5

GC content: 47.42

Gene sequence:

>1008_bases
ATGAATCTATGGAGAAGGATGATCACCGGTGTACTTTGCTTAATCTTCCTCTTTTCCCTTTTTACCCCTGTACACGCTAA
AGCGGATGATAGCGAAACCGCCTATGTAGATGTAGCGGTTGCCACTCTTTGGGTTGAACCGGGAATAACCCGAAGCATTG
ATTCTCCATCCCTGTCCAACCCGGTTGATCTATGGACATGGACGAAAAGTATGTCCTATGAAGAAAAACTGTGGCTAGTC
GGGAACCTTGAAACCCAAGCGCTATATGGCACCAAAGTAACGATATTGGAAAAACAAGGAGATTGGGTAAAAGTAGCCGT
TCACGGCCAACCGACCCCTCGCCATTCGCTTGGCTACCCAGGTTGGATGCCGATTCACCAACTGACAAAAGGAACAGCGT
TTTCCCAATTTCAGTCTAAGCCGTTTGCCCAAATCATCAAACCAACCGCTTGGCTTTACCATGATCCAAACAGCCGCAAA
CGGTTCATGGAAATCAGTTTTGCAACTCGTCTTCCAGTCATATCTACTACTAAGCACGCTGTAAAAGTGATGACACCTAG
CGACGGAGCAAAATGGTTGAAACGGGAAGACGTCCAAATTTTCCGGACTGAAGCAGACATCCCGACCCCTACTGGAGAAG
ATGTCGTTCGTACCGCCAAACAATTTTTAGGATTGCCATACTTATGGGCGGGGACGTCCGGGTTCGGGTTTGACTGTTCG
GGATTTACCCATACCATTTATAAAGCCCACGGTATTACCATTCCACGCGACTCATCAGTGCAGGCGCAGTTTGGCCTCCC
AGTTTCAGAAAAGGATTTACAACCGGGGGATCTCCTCTTCTTCGCCTATGATCAAGGCAAAGGAAGAGTACATCATGTAG
GGATGTATATTGGTAATGGAAAAATGATTCACTCTCCAAACTCTAGCAGTACGGTTCGTATCGATGACTACCGTGCTCCG
GGATATGGCGAGGAATTCGCAGGCGCTAGACGATACATTCAACGTTAA

Upstream 100 bases:

>100_bases
CAGCAACCAGTTCCCGTTCTTTCAAGGAAGCGAAATTCATCTTCCGAACTCTCCTGGGATTGGTATCTCCTATCCATCTT
AATTTAAAGGAGGAAATGCT

Downstream 100 bases:

>100_bases
TCTTTTCAGAGAATAGGCTGCTACAAATTCCTTTTCAGACTGTTGGATGACAAAAACAAAGAAAACCGTTCTTTTTCGGT
TGCGACAACGTAACCAAAAA

Product: polysugar degrading alpha-amylase

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNLWRRMITGVLCLIFLFSLFTPVHAKADDSETAYVDVAVATLWVEPGITRSIDSPSLSNPVDLWTWTKSMSYEEKLWLV
GNLETQALYGTKVTILEKQGDWVKVAVHGQPTPRHSLGYPGWMPIHQLTKGTAFSQFQSKPFAQIIKPTAWLYHDPNSRK
RFMEISFATRLPVISTTKHAVKVMTPSDGAKWLKREDVQIFRTEADIPTPTGEDVVRTAKQFLGLPYLWAGTSGFGFDCS
GFTHTIYKAHGITIPRDSSVQAQFGLPVSEKDLQPGDLLFFAYDQGKGRVHHVGMYIGNGKMIHSPNSSSTVRIDDYRAP
GYGEEFAGARRYIQR

Sequences:

>Translated_335_residues
MNLWRRMITGVLCLIFLFSLFTPVHAKADDSETAYVDVAVATLWVEPGITRSIDSPSLSNPVDLWTWTKSMSYEEKLWLV
GNLETQALYGTKVTILEKQGDWVKVAVHGQPTPRHSLGYPGWMPIHQLTKGTAFSQFQSKPFAQIIKPTAWLYHDPNSRK
RFMEISFATRLPVISTTKHAVKVMTPSDGAKWLKREDVQIFRTEADIPTPTGEDVVRTAKQFLGLPYLWAGTSGFGFDCS
GFTHTIYKAHGITIPRDSSVQAQFGLPVSEKDLQPGDLLFFAYDQGKGRVHHVGMYIGNGKMIHSPNSSSTVRIDDYRAP
GYGEEFAGARRYIQR
>Mature_335_residues
MNLWRRMITGVLCLIFLFSLFTPVHAKADDSETAYVDVAVATLWVEPGITRSIDSPSLSNPVDLWTWTKSMSYEEKLWLV
GNLETQALYGTKVTILEKQGDWVKVAVHGQPTPRHSLGYPGWMPIHQLTKGTAFSQFQSKPFAQIIKPTAWLYHDPNSRK
RFMEISFATRLPVISTTKHAVKVMTPSDGAKWLKREDVQIFRTEADIPTPTGEDVVRTAKQFLGLPYLWAGTSGFGFDCS
GFTHTIYKAHGITIPRDSSVQAQFGLPVSEKDLQPGDLLFFAYDQGKGRVHHVGMYIGNGKMIHSPNSSSTVRIDDYRAP
GYGEEFAGARRYIQR

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=117, Percent_Identity=38.4615384615385, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786421, Length=125, Percent_Identity=36, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788501, Length=114, Percent_Identity=35.0877192982456, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788001, Length=91, Percent_Identity=36.2637362637363, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 37646; Mature: 37646

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLWRRMITGVLCLIFLFSLFTPVHAKADDSETAYVDVAVATLWVEPGITRSIDSPSLSN
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCC
PVDLWTWTKSMSYEEKLWLVGNLETQALYGTKVTILEKQGDWVKVAVHGQPTPRHSLGYP
CCEEEEECCCCCCCCEEEEEECCCEEEEECCEEEEEECCCCEEEEEEECCCCCCCCCCCC
GWMPIHQLTKGTAFSQFQSKPFAQIIKPTAWLYHDPNSRKRFMEISFATRLPVISTTKHA
CCCHHHHHHCCCHHHHHHCCCHHHHHCCCEEEEECCCCCCEEEEEEHHHCCCEEECCCCE
VKVMTPSDGAKWLKREDVQIFRTEADIPTPTGEDVVRTAKQFLGLPYLWAGTSGFGFDCS
EEEECCCCCHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCC
GFTHTIYKAHGITIPRDSSVQAQFGLPVSEKDLQPGDLLFFAYDQGKGRVHHVGMYIGNG
CCEEEEEEECCEECCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEEECC
KMIHSPNSSSTVRIDDYRAPGYGEEFAGARRYIQR
EEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNLWRRMITGVLCLIFLFSLFTPVHAKADDSETAYVDVAVATLWVEPGITRSIDSPSLSN
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCC
PVDLWTWTKSMSYEEKLWLVGNLETQALYGTKVTILEKQGDWVKVAVHGQPTPRHSLGYP
CCEEEEECCCCCCCCEEEEEECCCEEEEECCEEEEEECCCCEEEEEEECCCCCCCCCCCC
GWMPIHQLTKGTAFSQFQSKPFAQIIKPTAWLYHDPNSRKRFMEISFATRLPVISTTKHA
CCCHHHHHHCCCHHHHHHCCCHHHHHCCCEEEEECCCCCCEEEEEEHHHCCCEEECCCCE
VKVMTPSDGAKWLKREDVQIFRTEADIPTPTGEDVVRTAKQFLGLPYLWAGTSGFGFDCS
EEEECCCCCHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCC
GFTHTIYKAHGITIPRDSSVQAQFGLPVSEKDLQPGDLLFFAYDQGKGRVHHVGMYIGNG
CCEEEEEEECCEECCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEEECC
KMIHSPNSSSTVRIDDYRAPGYGEEFAGARRYIQR
EEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]