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The map label for this gene is ykfB [H]

Identifier: 138896818

GI number: 138896818

Start: 3282899

End: 3283966

Strand: Direct

Name: ykfB [H]

Synonym: GTNG_3181

Alternate gene names: 138896818

Gene position: 3282899-3283966 (Clockwise)

Preceding gene: 138896816

Following gene: 138896819

Centisome position: 92.47

GC content: 45.04

Gene sequence:

>1068_bases
ATGAAAATAAAGCAGATCAACGTCACTACCCAATCCATTCCACTCGTGAAGCCATTTAAAACAGCCTTGCGAACAGTTAC
GCAAATTGAAAGCATCCTAGTCAAAGTCACCCTTGACAATGGGATTGAAGGATATGGAGCTACAGTTCCGACAGAAGCCA
TCACAGGGGAAACAAAACAAAGCATCATAGGAGTCTTGCAATATAGACTCATTCCGGTCGTCATCGGCCGCGATATTGAA
ATGATCGCGAAAAACACAAAAGATGTGCAAACATGTTGTGTCGGCAATACAAGTGCCAAAGCAGCACTAGACATGGCCAT
GCACGATGCACTTGGCAAACACTTAGGAATCCCCCTTTATCAATGGTTCGGTGGAAAAACTCATGATCATGTAAACGATA
TGACCATCAGTGTCAATCCAACAGAGCAGATGATTCGCGATGCAGAAATAGCAATCAACCACGGATTCTCCGTTCTAAAA
ATCAAAGTGGGGAAAGAGGCTAAAAAAGATATAGAACGAATCAAGCGGATTTACGAAGCGATTGGACCGAACGTAGTGCT
GCGCATTGATGCCAATCAAGGATGGACGGCAAAAGAAGCTGTTCGTATTATTCGTCAATTGGAACAAAGCAACCTCCCAA
TCGAGTTTATCGAACAACCTGTTCCGAAATATGATATTGAAGGATTGCGCTTTATTCGCGAACGAGTCAACATCCCCATT
ATGGCAGACGAGAGCGTGTTTTCTGCTCACGATGCTCTCACACTCATTCGCTGCCAAGCAGTGGATTTGATCAATATTAA
GCTGATGAAAACCGGAGGATTACGAGAAGCGTATACTATCGCCAGCCTTGCGGAATCAGCGGGCATACAATGCATGATTG
GAAGCATGATGGAACCGACACTTTCCGTATTAGCGGCAGCCCATTTAGCAGCGGCTCACCCCAACATTACAAGAGTCGAC
TTAGATGCGCCACTATGGCTCGATGACAGCAACCAGTTCCCGTTCTTTCAAGGAAGCGAAATTCATCTTCCGAACTCTCC
TGGGATTGGTATCTCCTATCCATCTTAA

Upstream 100 bases:

>100_bases
GGAAAATTCGCATTTTTTGTACCCTCTATTTTCTTTATTTTCCTACTGCGTTCTATGTCAATATATCAAGTACAACTTGT
GGTGAAGAGCCGTTTGATTC

Downstream 100 bases:

>100_bases
TTTAAAGGAGGAAATGCTATGAATCTATGGAGAAGGATGATCACCGGTGTACTTTGCTTAATCTTCCTCTTTTCCCTTTT
TACCCCTGTACACGCTAAAG

Product: muconate cycloisomerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKIKQINVTTQSIPLVKPFKTALRTVTQIESILVKVTLDNGIEGYGATVPTEAITGETKQSIIGVLQYRLIPVVIGRDIE
MIAKNTKDVQTCCVGNTSAKAALDMAMHDALGKHLGIPLYQWFGGKTHDHVNDMTISVNPTEQMIRDAEIAINHGFSVLK
IKVGKEAKKDIERIKRIYEAIGPNVVLRIDANQGWTAKEAVRIIRQLEQSNLPIEFIEQPVPKYDIEGLRFIRERVNIPI
MADESVFSAHDALTLIRCQAVDLINIKLMKTGGLREAYTIASLAESAGIQCMIGSMMEPTLSVLAAAHLAAAHPNITRVD
LDAPLWLDDSNQFPFFQGSEIHLPNSPGIGISYPS

Sequences:

>Translated_355_residues
MKIKQINVTTQSIPLVKPFKTALRTVTQIESILVKVTLDNGIEGYGATVPTEAITGETKQSIIGVLQYRLIPVVIGRDIE
MIAKNTKDVQTCCVGNTSAKAALDMAMHDALGKHLGIPLYQWFGGKTHDHVNDMTISVNPTEQMIRDAEIAINHGFSVLK
IKVGKEAKKDIERIKRIYEAIGPNVVLRIDANQGWTAKEAVRIIRQLEQSNLPIEFIEQPVPKYDIEGLRFIRERVNIPI
MADESVFSAHDALTLIRCQAVDLINIKLMKTGGLREAYTIASLAESAGIQCMIGSMMEPTLSVLAAAHLAAAHPNITRVD
LDAPLWLDDSNQFPFFQGSEIHLPNSPGIGISYPS
>Mature_355_residues
MKIKQINVTTQSIPLVKPFKTALRTVTQIESILVKVTLDNGIEGYGATVPTEAITGETKQSIIGVLQYRLIPVVIGRDIE
MIAKNTKDVQTCCVGNTSAKAALDMAMHDALGKHLGIPLYQWFGGKTHDHVNDMTISVNPTEQMIRDAEIAINHGFSVLK
IKVGKEAKKDIERIKRIYEAIGPNVVLRIDANQGWTAKEAVRIIRQLEQSNLPIEFIEQPVPKYDIEGLRFIRERVNIPI
MADESVFSAHDALTLIRCQAVDLINIKLMKTGGLREAYTIASLAESAGIQCMIGSMMEPTLSVLAAAHLAAAHPNITRVD
LDAPLWLDDSNQFPFFQGSEIHLPNSPGIGISYPS

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=344, Percent_Identity=27.6162790697674, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI48994953, Length=282, Percent_Identity=24.113475177305, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39069; Mature: 39069

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKQINVTTQSIPLVKPFKTALRTVTQIESILVKVTLDNGIEGYGATVPTEAITGETKQ
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCHHHCCCHHH
SIIGVLQYRLIPVVIGRDIEMIAKNTKDVQTCCVGNTSAKAALDMAMHDALGKHLGIPLY
HHHHHHHHHHHHHEECCCHHHHHCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHCCCCHH
QWFGGKTHDHVNDMTISVNPTEQMIRDAEIAINHGFSVLKIKVGKEAKKDIERIKRIYEA
HHCCCCCCCCCCCEEEEECCHHHHHHHHHEEEECCCEEEEEEECCHHHHHHHHHHHHHHH
IGPNVVLRIDANQGWTAKEAVRIIRQLEQSNLPIEFIEQPVPKYDIEGLRFIRERVNIPI
HCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCE
MADESVFSAHDALTLIRCQAVDLINIKLMKTGGLREAYTIASLAESAGIQCMIGSMMEPT
EECCHHHHHHHHHEEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCCEEEEHHHHHHH
LSVLAAAHLAAAHPNITRVDLDAPLWLDDSNQFPFFQGSEIHLPNSPGIGISYPS
HHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKIKQINVTTQSIPLVKPFKTALRTVTQIESILVKVTLDNGIEGYGATVPTEAITGETKQ
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCHHHCCCHHH
SIIGVLQYRLIPVVIGRDIEMIAKNTKDVQTCCVGNTSAKAALDMAMHDALGKHLGIPLY
HHHHHHHHHHHHHEECCCHHHHHCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHCCCCHH
QWFGGKTHDHVNDMTISVNPTEQMIRDAEIAINHGFSVLKIKVGKEAKKDIERIKRIYEA
HHCCCCCCCCCCCEEEEECCHHHHHHHHHEEEECCCEEEEEEECCHHHHHHHHHHHHHHH
IGPNVVLRIDANQGWTAKEAVRIIRQLEQSNLPIEFIEQPVPKYDIEGLRFIRERVNIPI
HCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCE
MADESVFSAHDALTLIRCQAVDLINIKLMKTGGLREAYTIASLAESAGIQCMIGSMMEPT
EECCHHHHHHHHHEEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCCEEEEHHHHHHH
LSVLAAAHLAAAHPNITRVDLDAPLWLDDSNQFPFFQGSEIHLPNSPGIGISYPS
HHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]